Gene targeting is enhanced in human cells overexpressing hRAD51

Gene Therapy

Volumn 6, Issue 7, 1999, Pages 1282-1290

  Yanez R J ^a   Porter, A C G ^a  

^a HAMMERSMITH HOSPITAL   (United Kingdom)

Author keywords

Gene targeting; Gene therapy; Homologous recombination; RAD genes; Radiation resistance

Indexed keywords

DNA; DNA BINDING PROTEIN;

ARTICLE; CONTROLLED STUDY; DNA REPAIR; GENE OVEREXPRESSION; GENE TARGETING; HUMAN; HUMAN CELL; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; RADIOSENSITIVITY;

BLOTTING, WESTERN; CELL LINE; DNA DAMAGE; DNA-BINDING PROTEINS; GENE EXPRESSION; GENE TARGETING; GENE THERAPY; HUMANS; HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE; RAD51 RECOMBINASE; RADIATION TOLERANCE; RECOMBINATION, GENETIC;

MAMMALIA;

EID: 0345711460     PISSN: 09697128     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.gt.3300945     Document Type: Article

References (55)

1. Yáñez RJ, Porter AC. Therapeutic gene targeting. Gene Therapy 1998; 5: 149-159.
2. Porter ACG. Designer genomes. Technique 1989; 1: 53-65.
3. Doi S, Campbell C, Kucherlapati R. Directed modification of genes by homologous recombination in mammalian cells. In: Grosveld F, Kollias G (eds). Transgenic Animals. Academic Press: London, 1992, pp 27-46.
4. Bollag RJ, Waldman AS, Liskay RM. Homologous recombination in mammalian cells. Annu Rev Genet 1989; 23: 199-225.
5. Mansour SL, Thomas KR, Capecchi MR. Disruption of the protooncogene int-2 in mouse embryo-derived stem cells: a general strategy for targeting mutations to non-selectable genes. Nature 1988; 336: 348-352.
6. Jasin M, Berg P. Homologous integration in mammalian cells without target gene selection. Genes Dev 1988; 2: 1353-1363.
7. Jasin M, de Villiers J, Weber F, Schaffner W. High frequency of homologous recombination in mammalian cells between endogenous and introduced SV40 genomes. Cell 1985; 43: 695-703.
8. Smithies O, Koralewski MA, Song KY, Kucherlapati RS. Homologous recombination with DNA introduced into mammalian cells. Cold Spring Harb Symp Quant Biol 1984; 49: 161-170.
9. Deng C, Capecchi MR. Reexamination of gene targeting frequency as a function of the extent of homology between the targeting vector and the target locus. Mol Cell Biol 1992; 12: 3365-3371.
10. Scheerer JB, Adair GM. Homology dependence of targeted recombination at the Chinese hamster APRT locus. Mol Cell Biol 1994; 14: 6663-6673.
11. Rouet P, Smih F, Jasin M. Introduction of double-strand breaks into the genome of mouse cells by expression of a rare-cutting endonuclease. Mol Cell Biol 1994; 14: 8096-8106.
12. Choulika A, Perrin A, Dujon B, Nicolas JF. Induction of homologous recombination in mammalian chromosomes by using the I-Scel system of Saccharomyces cerevisiae. Mol Cell Biol 1995; 15: 1968-1973.
13. Liang F et al. Chromosomal double-strand break repair in Ku80-deficient cells. Proc Natl Acad Sci USA 1996; 93: 8929-8933.
14. Smih F, Rouet P, Romanienko PJ, Jasin M. Double-strand breaks at the target locus stimulate gene targeting in embryonic stem cells. Nucleic Acids Res 1995; 23: 5012-5019.
15. Kunzelmann K et al. Gene targeting of CFTR DNA in CF epithelial cells. Gene Therapy 1996; 3: 859-867.
16. Kmiec EB. Targeted gene repair. Gene Therapy 1999; 6: 1-3.
17. Templeton NS, Roberts DD, Safer B. Efficient gene targeting in mouse embryonic stem cells. Gene Therapy 1997; 4: 700-709.
18. Russell DW, Hirata RK. Human gene targeting by viral vectors. Nat Genet 1998; 18: 325-330.
19. Shinohara A, Ogawa T. Homologous recombination and the roles of double-strand breaks. Trends Biochem Sci 1995; 20: 387-391.
20. Shinohara A et al. Cloning of human, mouse and fission yeast recombination genes homologous to RAD51 and recA. Nat Genet 1993; 4: 239-243.
21. Muris DF et al. Cloning of human and mouse genes homologous to RAD52, a yeast gene involved in DNA repair and recombination. Mutat Res 1994; 315: 295-305.
22. Kanaar R et al. Human and mouse homologs of the Saccharomyces cerevisiae RAD54 DNA repair gene: evidence for functional conservation. Curr Biol 1996; 6: 828-838.
23. Sung P. Catalysis of ATP-dependent homologous DNA pairing and strand exchange by yeast RAD51 protein. Science 1994; 265: 1241-1243.
24. Baumann P, Benson FE, West SC. Human RAD51 protein promotes ATP-dependent homologous pairing and strand transfer reactions in vitro. Cell 1996; 87: 757-766.
25. Essers J et al. Disruption of mouse RAD54 reduces ionizing radiation resistance and homologous recombination. Cell 1997; 89: 195-204.
26. Bezzubova O et al. Reduced X-ray resistance and homologous recombination frequencies in a RAD54-/-mutant of the chicken DT40 cell line. Cell 1997; 89: 185-193.
27. Yamaguchi-Iwai Y et al. Homologous recombination, but not DNA repair, is reduced in vertebrate cells deficient in RAD52. Mol Cell Biol 1998; 18: 6430-6435.
28. Rijkers T et al. Targeted inactivation of mouse RAD52 reduces homologous recombination but not resistance to ionizing radiation. Mol Cell Biol 1998; 18: 6423-6429.
29. Sonoda E et al. RAD51-deficient vertebrate cells accumulate chromosomal breaks prior to cell death. EMBO J 1998; 17: 598-608.
30. Lim DS, Hasty P. A mutation in mouse RAD51 results in an early embryonic lethal that is suppressed by a mutation in p53. Mol Cell Biol 1996; 16: 7133-7143.
31. Johnson BL et al. Elevated levels of recombinational DNA repair in human somatic cells expressing the Saccharomyces cerevisiae RAD52 gene. Mutat Res 1996; 363: 179-189.
32. Park MS. Expression of human RAD52 confers resistance to ionizing radiation in mammalian cells. J Biol Chem 1995; 270: 15467-15470.
33. Vispé S, Cazaux C, Lesca C, Defais M. Overexpression of Rad51 protein stimulates homologous recombination and increases resistance of mammalian cells to ionizing radiation. Nucleic Acids Res 1998; 26: 2859-2864.
34. Pennington SL, Wilson JH. Gene targeting in Chinese hamster ovary cells is conservative. Proc Natl Acad Sci USA 1991; 88: 9498-9502.
35. Taghian DG, Nickoloff JA. Chromosomal double-strand breaks induce gene conversion at high frequency in mammalian cells. Mol Cell Biol 1997; 17: 6386-6393.
36. Waldman AS, Liskay RM. Differential effects of base-pair mismatch on intrachromosomal versus extrachromosomal recombination in mouse cells. Proc Natl Acad Sci USA 1987; 84: 5340-5344.
37. Porter AC, Itzhaki JE. Gene targeting in human somatic cells. Complete inactivation of an interferon-inducible gene. Eur J Biochem 1993; 218: 273-281.
38. Melton DW. Gene targeting in the mouse. BioEssays 1994; 16: 633-638.
39. Itzhaki JE, Gilbert CS, Porter AC. Construction by gene targeting in human cells of a 'conditional' CDC2 mutant that rereplicates its DNA. Nat Genet 1997; 15: 258-265.
40. Xia SJ, Shammas MA, Reis RJ. Elevated recombination in immortal human cells is mediated by HsRAD51 recombinase. Mol Cell Biol 1997; 17: 7151-7158.
41. Thyagarajan B, McCormick Graham M, Romero DP, Campbell C. Characterization of homologous DNA recombination activity in normal and immortal mammalian cells. Nucleic Acids Res 1996; 24: 4084-4091.
42. Rice MC et al. Isolation of human and mouse genes based on homology to REC2, a recombinational repair gene from the fungus Ustilago maydis. Proc Natl Acad Sci USA 1997; 94: 7417-7422.
43. Dosanjh MK et al. Isolation and characterization of RAD51C, a new human member of the RAD51 family of related genes. Nucleic Acids Res 1998; 26: 1179-1184.
44. Pittman DL, Weinberg LR, Schimenti JC. Identification, characterization and genetic mapping of Rad51d, a new mouse and human RAD51/RecA-related gene. Genomics 1998; 49: 103-111.
45. Liu N et al. XRCC2 and XRCC3, new human Rad51-family members, promote chromosome stability and protect against DNA cross-links and other damages. Molec Cell 1998; 1: 783-793.
46. Sato S, Seki N, Hotta Y, Tabata S. Expression profiles of a human gene identified as a structural homologue of meiosis-specific recA-like genes. DNA Res 1995; 2: 183-186.
47. Waldman BC, Waldman AS. Illegitimate and homologous recombination in mammalian cells: differential sensitivity to an inhibitor of poly(ADP-ribosylation). Nucleic Acids Res 1990; 18: 5981-5988.
48. Waldman BC, O'Quinn JR, Waldman AS. Enrichment for gene targeting in mammalian cells by inhibition of poly(ADP-ribosylation). Biochim Biophys Acta 1996; 1308: 241-250.
49. Sambrook J, Fritsch EF, Maniatis T. Molecular Cloning. A Laboratory Manual. Cold Spring Harbor Laboratory Press: New York, 1989.
50. Southern PJ, Berg P. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter. J Mol Appl Genet 1982; 1: 327-341.
51. Andersson S et al. Cloning, structure, and expression of the mitochondrial cytochrome P-450 sterol 26-hydroxylase, a bile acid biosynthetic enzyme. J Biol Chem 1989; 264: 8222-8229.
52. Amaravadi L, King MW. A rapid and efficient, nonradioactive method for screening recombinant DNA libraries. BioTechniques 1994; 16: 98-103.
53. Edwards A et al. Automated DNA sequencing of the human HPRT locus. Genomics 1990; 6: 593-608.
54. Rasheed S et al. Characterization of a newly derived human sarcoma cell line (HT-1080). Cancer 1974; 33: 1027-1033.
55. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227: 680-685.