Use of a small molecule cell cycle inhibitor to control cell growth and improve specific productivity and product quality of recombinant proteins in CHO cell cultures

Biotechnology and Bioengineering

Volumn 112, Issue 1, 2015, Pages 141-155

  Du, Zhimei ^a   Treiber, David ^a   Mccarter, John D ^b   Fomina Yadlin, Dina ^a   Saleem, Ramsey A ^a   Mccoy, Rebecca E ^a   Zhang, Yuling ^a   Tharmalingam, Tharmala ^b   Leith, Matthew ^a   Follstad, Brian D ^a   Dell, Brad ^a   Grisim, Brent ^a   Zupke, Craig ^a   Heath, Carole ^a   Morris, Arvia E ^a   Reddy, Pranhitha ^a  

^a AMGEN INC   (United States)

^b AMGEN INC   (United States)

Author keywords

Glycosylation; Product quality; Recombinant antibody production; Specific productivity

Indexed keywords

ANTIBODIES; CELL CULTURE; CELL ENGINEERING; CELL GROWTH; CELL PROLIFERATION; CELLS; CLONE CELLS; CLONING; CYTOLOGY; ESTERIFICATION; GENE EXPRESSION; GLYCOSYLATION; GROWTH KINETICS; MOLECULES; PRODUCTIVITY; PROTEINS; QUALITY CONTROL; RECOMBINANT PROTEINS;

BIOPHARMACEUTICAL INDUSTRY; CHINESE HAMSTER OVARY CELLS; CULTURE TEMPERATURE; CYCLIN-DEPENDENT KINASE; HIGH MOLECULAR WEIGHT; RECOMBINANT ANTIBODY PRODUCTION; SELECTIVE INHIBITORS; SPECIFIC PRODUCTIVITY;

CELL SIGNALING;

CYCLIC AMP; CYCLIN DEPENDENT KINASE 2; CYCLIN DEPENDENT KINASE 4; CYCLIN DEPENDENT KINASE 6; GLYCAN; JANUS KINASE 1; JANUS KINASE 2; PROTEIN KINASE PIM 1; PROTEIN SERINE THREONINE KINASE; PROTEIN TYROSINE KINASE; RECOMBINANT PROTEIN; SOMATOMEDIN C RECEPTOR; CYCLIN DEPENDENT KINASE; ENZYME INHIBITOR;

ANIMAL CELL; ARTICLE; CELL CYCLE CHECKPOINT; CELL GROWTH; CELL PROLIFERATION; CHO CELL LINE; DNA MICROARRAY; FEMALE; G1 PHASE CELL CYCLE CHECKPOINT; GLYCOSYLATION; M PHASE CELL CYCLE CHECKPOINT; MICROARRAY ANALYSIS; MOLECULAR WEIGHT; NONHUMAN; PROCESS DEVELOPMENT; WESTERN BLOTTING; ANIMAL; ANTAGONISTS AND INHIBITORS; BIOREACTOR; CRICETULUS; CULTURE TECHNIQUE; DRUG EFFECTS; HAMSTER; METABOLISM; PROCEDURES;

ANIMALS; BIOREACTORS; CELL CULTURE TECHNIQUES; CELL CYCLE CHECKPOINTS; CHO CELLS; CRICETINAE; CRICETULUS; CYCLIN-DEPENDENT KINASES; ENZYME INHIBITORS; RECOMBINANT PROTEINS;

EID: 84911436420     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.25332     Document Type: Article

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