Fermentor temperature as a tool for control of high-density perfusion cultures of mammalian cells

Biotechnology and Bioengineering

Volumn 55, Issue 2, 1997, Pages 328-338

  Chuppa, Sandra ^a   Tsai, Yeong Shou ^a   Yoon, Sungjin ^a   Shackleford, Steve ^a   Rozales, Carl ^a   Bhat, Ramadas ^a   Tsay, Grace ^a   Matanguihan, Cary ^a   Konstantinov, Konstantin ^a   Naveh, David ^a  

^a BAYER CORPORATION   (United States)

Author keywords

Animal cell fermentation; Fermentor optimization; Perfusion culture; Temperature control

Indexed keywords

BIOREACTORS; FERMENTATION; FERMENTERS; GLUCOSE; GROWTH KINETICS; METABOLISM; OPTIMIZATION; PH EFFECTS; TEMPERATURE CONTROL; THERMAL EFFECTS;

FERMENTOR TEMPERATURE; PERFUSION CULTURE;

ANIMAL CELL CULTURE;

ANIMAL CELL; ANTIBODY PRODUCTION; ARTICLE; CELL CYCLE G1 PHASE; CELL DENSITY; CELL GROWTH; CELL METABOLISM; CONTROL SYSTEM; FERMENTATION; GLUCOSE METABOLISM; MAMMAL CELL; NONHUMAN; OXYGEN TRANSPORT; PERFUSION; THERMOREGULATION;

EID: 0342378192     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19970720)55:2<328::AID-BIT10>3.0.CO;2-D     Document Type: Article

References (15)

1. Adamson, R. 1994. Design and operation of a recombinant mammalian cell manufacturing process for rFVIII. Ann. Hematol. 68: 9-14.
2. Bloemkolk, J., Gray, M., Merchant, F., Mosmann, T. 1994. Effect of temperature on hybridoma cell cycle and MAb production. Biotechnol. Bioeng. 40: 427-431.
3. Enfors, S. 1992. Control of in vivo proteolysis in the production of recombinant proteins. Trends Biotechnology. 10: 310-315.
4. Hovey, A., Bebbington, C., Jenkins, N. 1994. Control of growth and recombinant protein synthesis by heat shock in a mutant mammalian cell line. Biotechnol. Lett. 16: 215-220.
5. Jenkins, N., Hovey, A. 1993. Temperature control of growth and productivity in mutant Chinese hamster ovary cells synthesizing a recombinant protein. Biotechnol. Bioeng. 42: 1029-1036.
6. Kratje, R., Lind, W., Wagner, R. 1994. Evaluation of the proteolytic potential of in vitro cultivated hybridoma and recombinant mammalian cells. J. Biotechnol. 32: 107-125.
7. Ludwig, A., Tomeczkowski, J., Kretzmer, G. 1992. Influence of the temperature on the shear stress sensitivity of adherent BHK21 cells. Appl. Microbiol. Biotechnol. 38: 323-327.
8. Merten, O., Reiter, S., Katinger, H. 1984. Stabilizing effect of reduced cultivation temperature on human-mouse hybridomas. Devel. Biol. Stand. 60: 509-512.
9. Passini, C., Gouchee, C. 1989. Response of a mouse hybridoma cell line to heat shock, agitation and sparging. Biotechnol. Progress. 5: 175-188.
10. Ramirez, O., Mutharasan, R. 1990. The role of the plasma membrane fluidity on the shear sensitivity of hybridoma growth under hydrodynamic stress. Biotechnol. Bioeng. 36: 911-920.
11. Rasmussen, P. (1991). Method for production of proteins with FVIII activity and/or FVIII derivatives. Patent WO 91/00347.
12. Reuveny, S., Velez, D., Macmillan, J., Miller, L. 1986. Factors affecting cell growth and monoclonal antibody production in stirred reactors. J. Immunol. Methods. 86: 53-59.
13. Sureshkumar, G., Mutharasan, R. 1991. The influence of temperature on a mouse-mouse hybridoma growth and monoclonal antibody production. Biotechnol. Bioeng. 37: 292-295.
14. Teige, M., Weidemann, R., Kretzmer, G. 1994. Problems with serum-free production of antithrombin III regarding proteolytic activity and product quality. J. Biotechnol. 34: 101-105.
15. Weidemann, R., Ludwig, A., Kretzmer, G. 1994. Low temperature cultivation - a step towards process optimization. Cytotechnology. 15: 111-116.