Stable microRNA expression enhances therapeutic antibody productivity of Chinese hamster ovary cells

Metabolic Engineering

Volumn 20, Issue , 2013, Pages 157-166

  Strotbek, Michaela ^a   Florin, Lore ^b   Koenitzer, Jennifer ^b   Tolstrup, Anne ^b   Kaufmann, Hitto ^b   Hausser, Angelika ^a   Olayioye, Monilola A ^a  

^a UNIVERSITY OF STUTTGART   (Germany)

^b BOEHRINGER INGELHEIM PHARMA GMBH AND CO KG   (Germany)

Author keywords

Cell line engineering; Fed batch culture; Functional genomic screen; MiRNA library; Recombinant therapeutic protein production

Indexed keywords

ANTIBODIES; BATCH CELL CULTURE; CELL ENGINEERING; CELLS; COMPLEX NETWORKS; CYTOLOGY; GENE EXPRESSION; INDUSTRIAL APPLICATIONS; PRODUCTIVITY; RNA;

CELL LINES; CHINESE HAMSTER OVARY CELLS; FED-BATCH CULTURES; FUNCTIONAL GENOMICS; RECOMBINANT HUMAN SERUM ALBUMIN; THERAPEUTIC ANTIBODIES; THERAPEUTIC PROTEIN; TRANSIENT TRANSFECTION;

MOLECULAR BIOLOGY;

GLYCAN; IMMUNOGLOBULIN G1 ANTIBODY; MICRORNA; MICRORNA 1287; MICRORNA 557; RECOMBINANT HUMAN SERUM ALBUMIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ANTIBODY PRODUCTION; ARTICLE; CELL DENSITY; CELL ENGINEERING; CHO CELL; CONTROLLED STUDY; ENGINEERING; ENZYME LINKED IMMUNOSORBENT ASSAY; FED BATCH CULTURE; FLOW CYTOMETRY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION; STABLE EXPRESSION; TRANSIENT TRANSFECTION;

CRICETULUS GRISEUS; MAMMALIA;

6-FAM (6-CARBOXYFLUORESCEIN); CAPILLARY GEL ELECTROPHORESIS; CELL LINE ENGINEERING; CGE; CHINESE HAMSTER OVARY; CHO; FAM; FED-BATCH CULTURE; FUNCTIONAL GENOMIC SCREEN; HOMO SAPIENS-MICRORNA; HSA; HSA-MIR; HUMAN SERUM ALBUMIN; MICRORNA; MIRNA; MIRNA LIBRARY; RECOMBINANT THERAPEUTIC PROTEIN PRODUCTION;

ANIMALS; ANTIBODIES, MONOCLONAL; CHO CELLS; CRICETINAE; CRICETULUS; GENOME-WIDE ASSOCIATION STUDY; HUMANS; IMMUNOGLOBULIN G; MICRORNAS;

EID: 84886827175     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2013.10.005     Document Type: Article

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