Controlled proliferation by multigene metabolic engineering enhances the productivity of Chinese hamster ovary cells

Nature Biotechnology

Volumn 16, Issue 5, 1998, Pages 468-472

  Fussenegger, Martin ^a   Schlatter, Stefan ^a   Dätwyler, Daniel ^a   Mazur, Xenia ^a   Bailey, James E ^a  

^a ETH ZURICH   (Switzerland)

Author keywords

Cell cycle; Protein synthesis

Indexed keywords

ALKALINE PHOSPHATASE; DNA BINDING PROTEIN; TETRACYCLINE;

ANIMAL CELL; ARTICLE; CELL CYCLE; CELL PROLIFERATION; CELL SURVIVAL; CHO CELL; CONTROLLED STUDY; GENETIC ENGINEERING; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION;

ALKALINE PHOSPHATASE; ANIMALS; APOPTOSIS; BLOTTING, WESTERN; CELL COUNT; CELL CYCLE; CELL DIVISION; CHO CELLS; CRICETINAE; GENES, BCL-2; GENETIC ENGINEERING; ONCOGENE PROTEIN P21(RAS); PROTEIN SYNTHESIS INHIBITORS; TETRACYCLINE;

EID: 0031945008     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/nbt0598-468     Document Type: Article

References (37)

1. Holmberg, N., Lilius, G., Bailey, J.E., and Bülow, L. 1997. Transgenic tabacco expressing Vitreoscilla hemoglobin exhibits enhanced growth and altered metabolic production. Nature Biotechnology 15:244-247.
2. Renner, W.A., Lee, K.H., Hatzimanikatis, V., Bailey, J.E., and Eppenberger, H.M. 1995. Recombinant cyclin E expression activates proliferation and obviates surface attachment of Chinese hamster ovary (CHO) cells in protein-free medium. Biotechnology and Bioengineering 47:476-482.
3. McDaniel, R., Ebert-Khosla, S., Hopwood, D.A., and Khosla, C. 1993. Engineered biosynthesis of novel polyketides. Science 262:1546-1550.
4. McDaniel. R., Ebert-Khosla. S., Hopwood, D.A., and Khosla, C. 1995. Rational design of aromatic polyketide natural products by recombinant assembly of enzymatic submits. Nature 375:549-554.
5. Alvarez, M.A., Fu, H., Khosla, C., Hopwood, D.A., and Bailey, J.E. 1996. Engineered biosynthesis of novel polyketides: properties of the whiE aromatase/cyclase. Nature Biotechnology 14:335-338.
6. Grana, X. and Reddy, E.P. 1995. Cell cycle control in mammalian cells: role of cyclins, cyclin-dependent kinases (CDKs), growth suppressor genes and cyclindependent kinase inhibitors (CKIs). Oncogene 11:211-219.
7. EI-Diery, W.W., Tokino, T., Velculescu, V.E., Levy, D.B., Parsons, R., Trent, Ü.M. et al. 1993. WAF1, a potential mediator of p53 tumor suppression. Cell 75:817-825.
8. Polyak, K., Lee, M.-L., Erdjument-Bromage, H., Koff, A., Roberts, J.M., Tempst, P., and Massagué, J. 1994. Cloning of p27Kip1, a cyclin-dependent kinase I inhibitor and a potential mediator of extracellular antimitogenic signals. Cell 78:59-66.
9. Toyoshima, H. and Hunter, T. 1994. p27, a novel inhibitor of G1 cyclin-Cdk protein kinase activity, is related to p21. Cell 78:67-74.
10. Harper, J.W., Adami, N., Wei, K., Keyomarsi, K., and Elledge, S.J. 1993. The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases. Ce// 75:805-816.
11. Xiong, Y., Hannon, G.J., Zhang, D., Casso, R., Kobayashi, R., and Beach, D. 1993. p21 is a universal inhibitor of cyclin kinases. Nature 366:701 -704.
12. Cartel, A.L., Serfas, M.S., and Tyner, A.L. 1996. p21-negative regulator of the cell cycle. Proceedings of Experimental and Biological Medicine 213:138-149.
13. Timchenko, N.A., Wilde, M., Nakanishi, M., Smith, J.R., and Darlington, G.J. 1996. CCAAT/enhancer-binding protein a (C/EBPa) inhibits cell proliferation through the p21 (WAF-1/CIP-1/SDI-1) protein. Genes Dev. 10:804-815.
14. Hartwell, L.H. and Kastan, M.B. 1994. Cell cycle control and cancer. Science 266:1821-1828.
15. Bertelsen, A.H., Beaudry, G.A., Stoller, T.J., Trotta, P.P., and Sherman, M.I. 1995. Tumor suppressor genes: prospects for cancer therapies. Bio/Technology 13:127-131.
16. Chen, J., Willingham, T., Shuford, M.,and Nisen, P.D. 1996. Tumor suppression and inhibition of aneuploid cell accumulation in human brain tumor cells by extopic overexpression of the cyclin-dependent kinase inhibitor p27-KIP1. J. Clin. Invest. 97:1983-1988.
17. dayman, G.L, Liu, T.J., Overholt, S.M., Mobley, S.R., Wang, M., Janot, F., and Goepfert, H. 1996. Gene therapy for head and neck cancer: Comparing the tumor suppressor gene p53 and a cell-cycle regulator WAF1/CIP1. Arch. Otolaryngol. Head Neck Surg. 122:489-493.
18. Pennisi, E. 1996. Will a twist of viral fate lead to a new cancer treatment? Science 274:342-343.
19. Yang, Z., Perkins, N.D., Ohno, T., Nabel, E.G., and Nabel, G.J. 1995. The p21 cyclin-dependent kinase-inhibitor suppresses tumorigenicity in vivo. Nat. Med. 1:1052-1056.
20. Fussenegger, M., Mazur, X., and Bailey, J.E. 1997. A novel cytostatic process enhances the productivity of Chinese hamster ovary cell. Biotechnology and Bioengineering 55:927-939.
21. Fussenegger, M., Mazur, X., Renner, W.A., and Bailey, J.E. 1998. Higher Productivity of growth-arrested Chinese hamster ovary (CHO) cells expressing cyclin-dependent kinase inhibitor gene p27. Biotechnology and Bioengineering. In press.
22. Rowan, S., Ludwig, R.L, Haupt, Y., Bates, S., Lu, X., Oren, M., and Vousden, K.H. 1996. Specific loss of apoptotic but not cell-cycle arrest function in a human tumor derived p53 mutant. EMBO J. 15:827-838.
23. Itoh, Y, Ueda, H., and Suzuki, E. 1995. Overexpression of bcl-2, apoptosis suppressing gene: prolonged viable culture period of hybridoma and enhanced antibody production. Biotechnology and Bioengineering 48:118-122.
24. Singh, P.P., Emery, A.N., and AI-Rubeai, M. 1996. Enhancement of survivability of mammalian cells by overexpression of the apoptosis-suppressor gene bc/-2. Biotechnology and Bioengineering 52:166-175.
25. Huang, D.C., Gory, S., and Strasser, A. 1997. Bcl-2, Bcl-xt and adenovirus protein E1B19KD are functionally equivalent in their ability to inhibit cell death. Oncogene 14:405-414.
26. Fussenegger, M., Mazur, X., and Bailey, J.E. 1998. pTRIDENT, a novel vector family for tricistronic gene expression in mammalian cells. Biotechnology and Bioengineering 57:1-10.
27. Minn, A.J., Boise, L.H., and Thompson, C.B. 1996. Expression of bcl-xt and loss of p53 can cooperate to overcome a cell cycle checkpoint induced by mitotic spindle damage. Genes Dev. 10:2621 -2631.
28. EI-Diery, W.S., Harper, J.W., O'Connor, P.M., Velculescu, V.E., Canman, C.E., Jackman, J. et al. 1994. WAF1/CIP1 is induced in p53-mediated G1 arrest and apoptosis. Cancer Res. 54:1169-1174.
29. Polyak, K., Kato, J.-Y, Solomon, M.J., Sherr, C.J., Massagué, J., Roberts, J.M., and Koff, A. 1994. p27Kip1, a cyclin-Cdk inhibitor, links transforming growth factor-ß and contact inhibition to cell-cycle arrest. Genes Dev. 8:9-22.
30. Yonish-Rouach, E., Resnitzky, D., Lotem, J., Sachs, L., Kimchi, A., and Qren, M. 1991. Wild-type p53 induces apoptosis of myeloid leukaemic cells that is inhibited by interIeukin-6. Nature 352:345-347.
31. Ko L.J. and Prives, C. 1996. p53: Puzzle and paradigm. Genes Dev. 10:1054-1072.
32. Gossen, M., and Bujard, H. 1992. Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc. Natl. Acad. Sci. USA 89:5547-5551.
33. Zang, M., Trautmann, H., Candor, C., Messi, F., Asselbergs, F., Leist, C. et al. 1995. Production of recombinant proteins in Chinese hamster ovary cells using a protein-free cell culture medium. Bio/Technology 13:389-392.
34. Berger, J., Hauber, J., Hauber, R., Geiger, R., and Cullen B.R. 1988. Secreted placental alkaline phosphatase: a powerful new quantitative indicator of gene expression in eukaryotic cells. Gene 66:1-10.
35. Behrens, T.W., Jagadeesh, J., Scherte, P., Dearns, G.M, Yewdell, J.W., and Staudt, L.M. 1994. Jaw1, a lymphoid-restricted membrane protein localized to the endoplasmic reticulum. J. Immunol. 153:682-690.
36. Tsujimoto, Y. and Grace, C.M. 1986. Analysis of the structure, transcripts, and protein products of bcl-2, the gene involved in human follicular lymphoma. Proc. Natl. Acad. Sci. USA. 83:5214-5218.
37. Boise, L.H., Gonzalez-Garcia, M., Postema, C.E., Ding, L., Lindsten, T., Turka, L.A. et al. 1993. bcl-x, a bc/-2-related gene that functions as a dominant regulator of apoptotic cell death. Cell 74:597-608.