Higher productivity of growth-arrested Chinese hamster ovary cells expressing the cyclin-dependent kinase inhibitor p27

Biotechnology Progress

Volumn 14, Issue 5, 1998, Pages 705-713

  Mazur, Xenia ^a   Fussenegger, Martin ^a   Renner, Wolfgang A ^a,b   Bailey, James E ^a  

^a ETH ZURICH   (Switzerland)

^b CYTOS BIOTECHNOLOGY AG   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

CELL CULTURE; GENE EXPRESSION;

ANIMAL CELL CULTURE; ENZYME INHIBITION;

ALKALINE PHOSPHATASE; DICISTRONIC EXPRESSION TECHNOLOGY; KINASE;

GENETIC ENGINEERING;

ALKALINE PHOSPHATASE; ANIMALS; BIOTECHNOLOGY; CELL CYCLE PROTEINS; CELL DIVISION; CHO CELLS; CRICETINAE; CYCLIN-DEPENDENT KINASE INHIBITOR P27; CYCLIN-DEPENDENT KINASES; ENZYME INHIBITORS; GENE EXPRESSION; GENES; GENETIC ENGINEERING; GENETIC VECTORS; MICROTUBULE-ASSOCIATED PROTEINS; PROMOTER REGIONS (GENETICS); PROTEINS; RECOMBINANT PROTEINS; TETRACYCLINE; TRANSFECTION; TUMOR SUPPRESSOR PROTEINS;

ANIMALIA; CRICETINAE; CRICETULUS GRISEUS;

EID: 0032170308     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp980062h     Document Type: Article

References (72)

1. Al-Rubeai, M.; Emery, A. N.; Chalder, S.; Jan, D. C. Specific monoclonal antibody productivity and the cell-cycle comparisons of batch, continuous and perfusion cultures. Cytotechnology 1992, 9, 85-97.
2. Barak, Y.; Juven, T.; Haffner, R.; Oren, M. mdm2 expression is induced by wild-type p53 activity. EMBO J. 1993, 12, 461-468.
3. Berger, J.; Hauber, J.; Hauber, R.; Geiger, R.; Cullen B. R. Secreted placental alkaline phosphatase: a powerful new quantitative indicator of gene expression in eukaryotic cells. Gene 1988, 66, 1-10.
4. Bertelsen, A. H.; Beaudry, G. A.; Stoller, T. J.; Trotta, P. P.; Sherman, M. I. Tumor suppressor genes: prospects for cancel therapies. Bio/Technology 1995, 13, 127-131.
5. Buell, D. N.; Fahey, J. L. Limited periods of gene expression in immunoglobulin-synthesizing cells. Science 1969, 164, 1524-1525.
6. Chabanas, A.; Lawrence, J. J.; Humbert, J.; Eisen, H. Cell cycle regulation of histone H1 in CHO cells: A flow cytofluorimetric study after double staining of cells. EMBO J. 1983, 2, 833-837.
7. Chafouleas, J. G.; Lagace, L.; Bolton, W. E.; Boyd, A. E., III; Means, A. R. Changes in calmodulin and its mRNA accompany reentry of quiescent (Go) cells into the cell-cycle. Cell 1984, 36, 73-81.
8. Chen, J.; Willingham, T.; Shuford, M.; Nisen, P. D. Tumor suppression and inhibition of aneuploid cell accumulation in human brain tumor cells by extopic overexpression of the cyclin-dependent kinase inhibitor p27-KIP1. J. Clin. Invest. 1996, 97, 1983-1988.
9. Clayman, G. L.; Liu, T. J.; Overholt, S. M.; Mobley, S. R.; Wang, M.; Janot, F.; Goepfert, H. Gene therapy for head and neck cancer: Comparing the tumor suppressor gene p53 and a cell-cycle regulator WAF1/CIP1. Arch. Otolaryngol., Head Neck Surg. 1996, 122, 489-493.
10. Cocket M. I.; Bebbington, C. R.; Yarranton, G. T. High level expression of tissue inhibitor of metalloproteinases in Chinese hamster ovary cells using glutamine synthetase gene amplification. Bio/Technology 1990, 8, 662-667.
11. Kip1 induces cell cycle arrest and loss of cyclin-Cdk activity in human breast cancer cells. Oncogene 1997, 14, 2283-2289.
12. Datto, M. B.; Li, Y.; Panus, J. F.; Howe, D. J.; Xiong, Y.; Wang, X.-F. Transforming growth factor b induces the cyclin-dependent kinase inhibitor p21 trough a p53-independent mechanisim. Proc. Natl. Acad. Sci. 1995, 92, 5545-5549.
13. El-Diery, W. W.; Tokino, T.; Velculescu, V. E.; Levy, D. B. Parsons, R.; Trent, J. M.; Lin, D.; Mercer, W. E.; Kinzler, K. W.; Vogelstein, B. WAF1, a potential mediator of p53 tumor suppression. Cell 1993, 75, 817-825.
14. El-Diery, W. S.; Harper, J. W.; O'Connor, P. M.; Velculescu, V. E.; Canman, C. E.; Jackman, J.; Pietenpol, J. A.; Burrell, M.; Hill, D. E.; Wang, Y.; Wiman, K. G.; Mercer, W. E.; Kastan, M. B.; Kohn, K. W.; Elledge, S. J.; Kinzler, K. W.; Vogelstein, B. WAF1/CIP1 is induced in p53-mediated G1 arrest and apoptosis. Cancer Res. 1994, 54, 1169-1174.
15. Flores-Rozas, H.; Kleman, Z.; Dean, F. B.; Pan, Z.-Q.; Harper, J. W.; Elledge, S. H.; O'Donnel, M.; Hurwitz. Cdk-interacting protein 1 directly binds with proliferating cell nuclear antigen and inhibits DNA replication catalyzed by the DNA polymerase d holoenzyme. Proc. Natl. Acad. Sci. 1994, 91, 8655-8659.
16. Fussenegger, M.; Mazur, X.; Bailey, J. E. A novel cytostatic process enhances the productivity of Chinese hamster ovariy cell. Biotechnol. Bioeng. 1997, 55, 927-939.
17. Fussenegger, M.; Mazur, X.; Bailey, J. E. pTRIDENT, a novel vector family for tricistronic gene expression in mammalian cells. Biotechnol. Bioeng, 1998, 57, 1-10.
18. Garatun-Tjeldsto, O.; Pryme, I. F.; Weltman, J. K.; Dowben, R. M. Synthesis and secretion of light-chain immunoglobulin in two successive cycles of synchronized plasmacytoma cells. J. Cell. Biol. 1976, 68, 232-239.
19. Gartel, A. L.; Serfas, M. S.; Tyner, A. L. p21-negative regulator of the cell cycle. Proc. Exp. Biol. Med. 1996, 213, 138-149.
20. Gartenhaus, R. B.; Wang, P.; Hoffmann, P. Induction of the WAF1/CIP1 protein and apoptosis in human T-cell leukemia virus I-transformed lymphocytes after treatment with Adriamycin by using a p53-independent pathway. Proc. Natl. Acad. Sci. 1996, 93, 265-268.
21. Givol, I.; Givol, D.; Rulong, S.; Resau, J.; Tsarfaty, I.; Hughes, S. H. Overexpression of human p21-waf/cip1 arrests the growth of chicken embryo fibroblasts transformed by individual oncogenes. Oncogene 1995, 11, 2609-2618.
22. Gossen, M.; Bujard, H. Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc. Natl. Acad. Sci. 1992, 89, 5547-5551.
23. Graña, X.; Reddy, E. P. Cell cycle control in mammalian cells: role of cyclins, cyclin-dependent kinases (CDKs), growth suppressor genes and cyclin-dependent kinase inhibitors (CKIs). Oncogene 1995, 11, 211-219.
24. Greenblatt, M. S.; Bennett, W. P.; Hollstein, M.; Harris, C. C. Mutations in the p53 tumor suppressor gene: Clues to cancer etiology and molecular pathogenesis. Cancer Res. 1994, 54, 4855-4878.
25. Gu, M. B.; Todd, P.; Kompala, D. S. Foreign gene expression (β-galactosidase) during the cell-cycle phases in recombinant CHO cells. Biotechnol. Bioeng. 1993, 42, 1113-1123.
26. Harper, J. W.; Adami, N.; Wei, K.; Keyomarsi, K.; Elledge, S. J. The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases. Cell 1993, 75, 805-816.
27. Hartwell, L. H.; Kastan, M. B. Cell cycle control and cancer. Science 1994, 266, 1821-1828.
28. cdc2 mitotic B cyclin complex. Cell 1994, 78, 813-822.
29. Hayter, P. M.; Curling, E. M. A.; Baines, A. J.; Jenkins, N.; Salmon, I.; Strange, P. G.; Bull, A. T. Chinese hamster ovary growth and interferon production kinetics in stirred batch culture. Appl. Microbiol. Biotechnol. 1991, 34, 559-564.
30. Herz, F.; Czerniak, B.; Deitch, D.; Wersto, R. P.; Simmons, D. A.; Koss, L. G. Protein expression in relation to the cell-cycle of exponentially growing human prostatic epithelial cells. Cell Prolif. 1991, 24, 321-330.
31. Jenkins, N.; Hovey, A. Temperature control of growth and productivity in mutant Chinese hamster ovary cell synthesising a recombinant protein. Biotechnol. Bioeng. 1993, 42, 1029-1036.
32. Kastan, M. B.; Onyekwere, O.; Sidransky, D.; Vogelstein, B.; Craig, R. W. Participation of p53 protein in the cellular response to DNA damage. Cancer Res. 1991, 51, 6304-6311.
33. Kirchhoff, S.; Koromilas, A. E.; Schaper, F.; Grashoff, M.; Sonenberg, N.; Hauser, H. IRF-1 induced cell growth inhibi- tion and interferon induction requires the activity of the protein kinase PKR. Oncogene 1995, 11, 439-445.
34. Ko, L. J.; Prives, C. p53: puzzle and paradigm. Genes Dev. 1996, 10, 1054-1072.
35. Köster, M.; Kirchhoff, S.; Schaper, F.; Hauser, H. Proliferation control of mammalian cells by the tumor suppressor IRF-1. Cytotechnology I 1995, 18, 67-75.
36. Kubbies, M.; Stockinger, H. Cell cycle-dependent DHFR and t-PA production in cotransfected, MTX-amplified CHO cells revealed by dual-laser flow cytometry. Exp. Cell Res. 1990, 188, 267-271.
37. Lane, D. P. Cancer. p53, guardian of the genome. Nature 1992, 358, 15-16.
38. Lee, K. H.; Sburlati, A.; Renner, W. A.; Bailey, J. E. Deregulated expression of cloned transcription factor E2F-1 in Chinese hamster ovary cells shifts protein patterns and activates growth in protein-free medium. Biotechnol. Bioeng. 1996, 50, 273-279.
39. Lewis, P. F.; Emermann, M. Passage through mitosis is required for oncoretroviruses but not for the human immunodeficiency virus. J. Virol. 1994, 68, 510-516.
40. 1 cell-cycle arrest induced by ribonucleotide depletion in the absence of detectable DNA damage. Genes Dev. 1996, 10, 934-947.
41. Liu, T. J.; Zhang, W. W.; Taylor, D. L.; Roth, J. A.; Goepfert, H.; Clayman, G. L. Growth suppression of human head and neck cancer cells by the introduction of a wild-type p53 gene via a recombinant adenovirus. Cancer Res. 1994, 54, 3662-3667.
42. Liu, T. J.; el-Naggar, A. K.; McDonnell, T. J.; Steck, K. D.; Wang, M.; Taylor, D. L.; Clayman, G. L. Apoptosis induction mediated by wild-type p53 adenoviral gene transfer is squamous cell carcinoma of the head and neck. Cancer Res. 1995, 55, 3117-3122.
43. Miller, A. D.; Miller, D. G.; Garcia, J. V.; Lynch, C. M. Use of retroviral vectors for gene transfer and expression. Methods Enzymol. 1993, 217, 581-599.
44. Miyashita, T.; Reed, J. C. Tumor suppressor p53 is a direct transcriptional activator of the human bax gene. Cell 1995, 80, 293-299.
45. Miyashita, T.; Harigai, M.; Kanaka, M.; Reed, J. C. Identification of a p53-dependent negative response element in the bcl-2 gene. Cancer Res. 1994, 54, 3131-3135.
46. Mulligan, R. C. The basic science of gene therapy. Science 1993, 260, 926-932.
47. Nurse, P. Ordering S phase and M phase in the cell cycle. Cell 1994, 79, 547-550.
48. Owen-Schaub, L. B.; Zhang, W.; Cusack, J. C.; Angelo, L. S.; Santee, S. M.; Fujiwara, T.; Roth, J. A.; Deisseroth, A. B.; Zhang, W.-W.; Kruzel, E.; Radinsky, R. Wild-type human p53 and a temperature-sensitive mutant induce fas/APO-1 expression. Mol. Cell. Biol. 1995, 15, 3032-3040.
49. Pendse, G. J.; Karkare, S.; Bailey, J. E. Effect of cloned gene dosage on cell growth and hepatitits B surface antigen in recombinant CHO cells. Biotechnol. Bioeng. 1992, 40, 119-129.
50. Pennisi, E. Will a twist of viral fate lead to a new cancer treatment? Science 1996, 274, 342-343.
51. Pine, A. Constitutive expression of an ISGF/IRF1 transgene leads to interferon-independent activation of interferon-inducible genes and resistance to virus infection. J. Virol. 1992, 66, 4470-4478.
52. Polyak, K.; Lee, M.-L.; Erdjument-Bromage, H.; Koff, A.; Roberts, J. M.; Tempst, P.; Massagué, J. Cloning of p27Kip1, a cyclin-dependent kinase inhibitor and a potential mediator of extracellular antimitogenic signals. Cell 1994a, 78, 59-66.
53. Polyak, K.; Kato, J.-Y.; Solomon, M. J.; Sherr, C. J.; Massagué, J.; Roberts, J. M.; Koff, A. p27Kip1, a cyclin-Cdk inhibitor, links transforming growth factor-b and contact inhibition to cell-cycle arrest. Genes Dev. 1994b, 8, 9-22.
54. Polyak, K.; Waldman, T.; He, T.-C.; Kinzler, K. W.; Vogelstein, B. Genetic determinants of p53-induced apoptosis and growth arrest. Genes Dev. 1996, 10, 1945-1952.
55. Renner, W. A.; Lee, K. H.; Hatzimanikatis, V.; Bailey, J. E.; Eppenberger, H. H. Recombinant cyclin E expression activates proliferation and obviates surface attachment of Chinese hamster ovary (CHO) cells in protein-free medium. Biotechnol. Bioeng. 1995, 47, 476-482.
56. Reynisdottir, I.; Polyak, K.; Iavarone, A.; Massagué, J. Kip/Cip and Ink4 inhibitors cooperate to induce cell-cycle arrest in response to TGF-b. Genes Dev. 1995, 9, 1831-1845.
57. Robinson, D. K.; Memmert, K. W. Kinetics of recombinant immunoglobulin production by mammalian cells in continuous culture. Biotechnol. Bioeng. 1991, 38, 972-976.
58. Roe, T.; Reynolds, T. C.; Yu, G.; Brown, P. O. Integration of murine leukemia virus DNA depends on mitosis. EMBO J. 1993, 72, 2099-2108.
59. Rowan, S.; Ludwig, R. L.; Haupt, Y.; Bates, S.; Lu, X.; Oren, M.; Vousden, K. H. Specific loss of apoptotic but not cell-cycle arrest function in a human tumor derived p53 mutant. EMBO J. 1996, 15, 827-838.
60. Sanders Williams, R. Human gene therapy - of tortoises and hares. Nature Med. 1995, 1, 1137-1138.
61. Sekiguchi, T.; Hunter, T. Induction of growth arrest and cell death by overexpression of the cyclin-Cdk inhibitor p21 in hamster BHK21 cells. Oncogene 1998, 369-380.
62. Shaw, P.; Bovey, R.; Tardy, S.; Sahli, R.; Sordat, B.; Costa, J. Induction of apoptosis by wild-type p53 in a human colon tumor-derived cell line. Proc. Natl. Acad. Sci. 1992, 89, 4495-4499.
63. Smiley, A. L.; Hu W.-S.; Wang, D. I. C. Production of human immune interferon genes and by recombinant mammalian cells cultivated on microcarriers. Biotechnol. Bioeng. 1989, 33, 1182-1190.
64. Suzuki, E.; Ollis, D. F. Enhanced antibody production at slowed growth rates: experimental demonstration and a simple structured model. Biotechnol. Prog. 1990, 6, 231-236.
65. Symonds, H.; Krall, L.; Remington, L.; Saenz-Robles, M.; Lowe, S.; Jacks, T.; Van Dyke, T. p53-independent apoptosis suppresses tumor growth and progression in vivo. Cell 1994, 78, 703-711.
66. Tanaka, N.; Ishihara, M.; Lamphier, M. S.; Nozawa, H.; Matsuyama, T.; Mak, T. W.; Aizawa, S.; Tokino, T.; Oren, M.; Taniguchi, T. Cooperation of the tumour suppressors IRF-1 and p53 in response to DNA damage. Nature 1996, 382, 816-818.
67. Xiong, Y.; Hannon, G. J.; Zhang, D.; Casso, R.; Kobayashi, R.; Beach, D. p21 is a universal inhibitor of cyclin kinases. Nature 1993a, 366, 701-704.
68. Xiong, Y.; Zhang, H.; Beach, D. Subunit rearrangement of the cyclin-dependent kinases is associated with cellular transformation. Genes Dev. 1993b, 7, 1572-1583.
69. Yang, C.; Cirielli, C.; Capogrosi, M. C.; Passaniti, A. Adenovirus-mediated wild-type p53 expression induces apoptosis and suppresses tumorigenesis of prostatic tumor cells. Cancer Res. 1995, 55, 4210-4213.
70. Yang, Z.; Perkins, N. D.; Ohno, T.; Nabel, E. G.; Nabel, G. J. The p21 cyclin-dependent kinase-inhibitor suppresses tumorigenicity in vivo. Nat. Med. 1995, 1, 1052-1056.
71. Yonish-Rouach, E.; Resnitzky, D.; Lotem, J.; Sachs, L.; Kimchi, A.; Qren, M. Wild-type p53 induces apoptosis of myeloid leukaemic cells that is inhibited by interleukin-6. Nature 1901, 352, 345-347.
72. Zang, M.; Trautmann, H.; Gandor, C.; Messi, F.; Asselbergs, F.; Leist, C.; Fiechter, A.; Reiser, J. Production of recombinant proteins in Chinese hamster ovary cells using a protein-free cell culture medium. Bio/Technology 1995, 13, 389-392.