Effect of Bcl-2 overexpression on cell cycle and antibody productivity in chemostat cultures of myeloma NS0 cells

Journal of Bioscience and Bioengineering

Volumn 100, Issue 3, 2005, Pages 303-310

  Tey, Beng Ti ^a   Al Rubeai, Mohamed ^b  

^a UNIVERSITY PUTRA MALAYSIA   (Malaysia)

^b UNIVERSITY COLLEGE DUBLIN   (Ireland)

Author keywords

Apoptosis; bcl 2; Bioreactor; Chemostat culture; NS0 myeloma cells

Indexed keywords

ANTIBODIES; CELL CULTURE; CORRELATION METHODS;

APOPTOSIS; CHEMOSTAT CULTURE; NS0 MYELOMA CELLS;

CELLS;

PROTEIN BCL 2;

ANIMAL CELL; ANTIBODY PRODUCTION; ARTICLE; BIOREACTOR; CELL CULTURE; CELL CYCLE; CELL CYCLE G1 PHASE; CELL DENSITY; CELL LINE; CELL POPULATION; CHEMOSTAT; CONTROLLED STUDY; CORRELATION ANALYSIS; DILUTION; GROWTH RATE; MOUSE; MYELOMA CELL; NONHUMAN; PROTEIN EXPRESSION;

ANIMALS; ANTIBODIES, MONOCLONAL; BIOREACTORS; BIOTECHNOLOGY; CELL CYCLE; CELL LINE, TUMOR; HYBRIDOMAS; MICE; MULTIPLE MYELOMA; PROTO-ONCOGENE PROTEINS C-BCL-2; TRANS-ACTIVATION (GENETICS); TRANSFECTION;

ANIMALIA;

EID: 21644431817     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1263/jbb.100.303     Document Type: Article

References (40)

1. Tey B.T., Singh R.P., Al-Rubeai M. Programmed cell death - an overview of apoptosis in cell culture Asia Pac. J. Mol. Biol. Biotechnol. 9 2001 1 28
2. Slee E.A., Adrain C., Martin S.J. Serial killers: ordering caspase activation events in apoptosis Cell Death Differ. 6 1999 1067 1074
3. Tey B.T., Al-Rubeai M. Bcl-2 over-expression reduced the serum dependency and improved the nutrient metabolism in NS0 cells culture Biotechnol. Bioprocess Eng. 10 2005 254 261
4. Itoh Y., Ueda H., Suzuki E. Over-expression of Bcl-2, apoptosis suppressing gene-prolonged viable culture period of hybridoma and enhanced antibody-production Biotechnol. Bioeng. 48 1995 118 122
5. Simpson N.H., Milner A.E., Al-Rubeai M. Prevention of hybridoma cell death by Bcl-2 during suboptimal culture conditions Biotechnol. Bioeng. 54 1997 1 16
6. Fujita T., Terada S., Fukuoka K., Kitayama A., Ueda H., Suzuki E. Reinforcing apoptosis-resistance of COS and myeloma cells by transfecting with bcl-2 gene Cytotechnology 25 1997 25 33
7. Tey B.T., Singh R.P., Piredda L., Piacentini M., Al-Rubeai M. Influence of bcl-2 on cell death during the cultivation of a Chinese hamster ovary cell line expressing a chimeric antibody Biotechnol. Bioeng. 68 2000 31 43
8. Goswami J., Sinskey A.J., Steller H., Stephanopoulos G.N., Wang D.I.C. Apoptosis in batch cultures of Chinese hamster ovary cells Biotechnol. Bioeng. 62 1999 632 640
9. Tey B.T., Singh R.P., Piredda L., Piacentini M., Al-Rubeai M. Bcl-2 mediated suppression of apoptosis in myeloma NS0 cultures J. Biotechnol. 79 2000 147 159
10. Tey B.T., Al-Rubeai M. Suppression of apoptosis in perfusion culture of myeloma NS0 cells enhances cell growth but reduces antibody productivity Apoptosis 9 2004 843 852
11. Bierau H., Perani A., Al-Rubeai M., Emery A.N. A comparison of intensive cell culture bioreactors operating with hybridomas modified for inhibited apoptotic response J. Biotechnol. 62 1998 195 207
12. Simpson N.H., Singh R.P., Emery A.N., Al-Rubeai M. Bcl-2 over-expression reduces growth rate and prolongs G(1) phase in continuous chemostat cultures of hybridoma cells Biotechnol. Bioeng. 64 1999 174 186
13. Al-Rubeai M., Emery A.N. Mechanisms and kinetics of monoclonal-antibody synthesis and secretion in synchronous and asynchronous hybridoma cell-cultures J. Biotechnol. 16 1990 67 86
14. Lloyd D.R., Al-Rubeai M. Cell cycle Flickinger M.C. Drew S.W. The encyclopedia of bioprocess technology: fermentation, biocatalysis and bioseparation 1999 John Wiley and Sons New York 465 476
15. Murray A. Cell-cycle checkpoints Curr. Opin. Cell Biol. 6 1994 872 876
16. Hung D.T., Jamison T.F., Schreiber S.L. Understanding and controlling the cell cycle with natural products Chem. Biol. 3 1996 623 639
17. King K.L., Cidlowski J.A. Cell cycle regulation and apoptosis Annu. Rev. Physiol. 60 1998 601 617
18. Brady H.J.M., GilGomez G., Kirberg J., Berns A.J.M. Bax alpha perturbs T cell development and affects cell cycle entry of T cells EMBO J. 15 1996 6991 7001
19. Brady H.J.M., Salomons G.S., Bobeldijk R.C., Berns A.J.M. T cells from Bax alpha transgenic mice show accelerated apoptosis in response to stimuli but do not show restored DNA damage-induced cell death in the absence of p53 EMBO J. 15 1996 1221 1230
20. Mazel S., Burtrum D., Petrie H.T. Regulation of cell division cycle progression by Bcl-2 expression: a potential mechanism for inhibition of programmed cell death J. Exp. Med. 183 1996 2219 2226
21. OReilly L.A., Huang D.C.S., Strasser A. The cell death inhibitor Bcl-2 and its homologues influence control of cell cycle entry EMBO J. 15 1996 6979 6990
22. Vairo G., Innes K.M., Adams J.M. Bcl-2 has a cell cycle inhibitory function separable from its enhancement of cell survival Oncogene 13 1996 1511 1519
23. Linette G.P., Li Y., Roth K., Korsmeyer S.J. Cross talk between cell death and cell cycle progression: Bcl-2 regulates NFAT-mediated activation Proc. Natl. Acad. Sci. USA 93 1996 9545 9552
24. Pietenpol J.A., Papadopoulos N., Markowitz S., Willson J.K.V., Kinzler K.W., Vogelstein B. Paradoxical inhibition of solid tumor-cell growth by Bcl2 Cancer Res. 54 1994 3714 3717
25. Ryan J.J., Prochownik E., Gottlieb C.A., Apel I.J., Merino R., Nunez G., Clarke M.F. c-Myc and Bcl-2 modulate p53 function by altering p53 subcellular trafficking during the cell cycle Proc. Natl. Acad. Sci. USA 91 1994 5878 5882
26. Uhlmann E.J., DSaEipper C., Subramanian T., Wagner A.J., Hay N., Chinnadurai G. Deletion of a nonconserved region of Bcl-2 confers a novel gain of function: suppression of apoptosis with concomitant cell proliferation Cancer Res. 56 1996 2506 2509
27. Huang D.C.S., OReilly L.A., Strasser A., Cory S. The anti-apoptosis function of Bcl-2 can be genetically separated from its inhibitory effect on cell cycle entry EMBO J. 16 1997 4628 4638
28. Follstad B.D., Balcarcel R.R., Stephanopoulos G., Wang D.I.C. Metabolic flux analysis of hybridoma continuous culture steady state multiplicity Biotechnol. Bioeng. 63 1999 675 683
29. Hayter P.M., Curling E.M.A., Gould M.L., Baines A.J., Jenkins N., Salmon I., Strange P.G., Bull A.T. The effect of the dilution rate on CHO cell physiology and recombinant interferon in glucose-limited chemostat culture Biotechnol. Bioeng. 42 1993 1077 1085
30. Lee Y.K., Yap P.K., Teoh A.P. Correlation between steady-state cell concentration and cell-death of hybridoma cultures in chemostat Biotechnol. Bioeng. 45 1995 18 26
31. Linardos T.I., Kalogerakis N., Behie L.A. Cell-cycle model for growth rate and death rate in continuous suspension hybridoma cultures Biotechnol. Bioeng. 40 1992 359 368
32. Martens D.E., Degooijer C.D., Vanderveldendegroot C.A.M., Beuvery E.C., Tramper J. Effect of dilution rate on growth, productivity, cell cycle and size, and shear sensitivity of a hybridoma cell in a continuous culture Biotechnol. Bioeng. 41 1993 429 439
33. Suzuki E., Ollis D.F. Cell-cycle model for antibody-production kinetics Biotechnol. Bioeng. 34 1989 1398 1402
34. Ray N.G., Karkare S.B., Runstadler P.W. Cultivation of hybridoma cells in continuous cultures - kinetics of growth and product formation Biotechnol. Bioeng. 33 1989 724 730
35. 2cell cycle arrest and apoptosis are induced in Burkitt's lymphoma cells by the anticancer agent oracin FEBS Lett. 400 1997 127 130
36. 2checkpoint is maintained by redundant pathways Mol. Cell. Biol. 19 1999 5872 5881
37. 2/M arrest and apoptosis Nat. Med. 2 1996 72 79
38. Hung D.T., Jamison T.F., Schreiber S.L. Understanding and controlling the cell cycle with natural products Chem. Biol. 3 1996 623 639
39. Bebbington C.R., Hentschel C.G. The use of vectors based on gene amplication for the expression of cloned genes in mammalian cells Glover D.M. DNA cloning: a practical approach vol. 3 1987 IRL Oxford 163 187
40. Marvel J., Perkins G.R., Lopez Rivas A., Collins M.K. Growth factor starvation of bcl-2 overexpressing murine bone marrow cells induced refractoriness to IL-3 stimulation of proliferation Oncogene 9 1994 1117 1122