Effect of low culture temperature on specific productivity, transcription level, and heterogeneity of erythropoietin in Chinese hamster ovary cells

Biotechnology and Bioengineering

Volumn 82, Issue 3, 2003, Pages 289-298

  Yoon, Sung Kwan ^a   Song, Ji Yong ^b   Lee, Gyun Min ^a  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

^b LG Chemical Ltd   (South Korea)

Author keywords

CHO cells; Erythropoietin (EPO); Heterogeneity of EPO glycoforms; Low culture temperature; Specific EPO productivity

Indexed keywords

CELL CULTURE; ENZYMES; RNA;

BIOLOGICAL ACTIVITY;

BIOMEDICAL ENGINEERING;

ERYTHROPOIETIN;

ANIMAL CELL; ARTICLE; BACTERIUM CULTURE; CELL TYPE; CELL VIABILITY; CHINESE HAMSTER; GENETIC HETEROGENEITY; GENETIC TRANSCRIPTION; ISOELECTRIC FOCUSING; LONGEVITY; NONHUMAN; OVARY CELL; PRODUCTIVITY; PROTEIN DEGRADATION; RNA ANALYSIS; SIALYLATION; TRANSCRIPTION REGULATION;

ANIMALS; CELL CULTURE TECHNIQUES; CELL DIVISION; CELL SURVIVAL; CHO CELLS; COLD; CRICETINAE; ERYTHROPOIETIN; GENE EXPRESSION REGULATION; HUMANS; RECOMBINANT PROTEINS; RNA, MESSENGER; SENSITIVITY AND SPECIFICITY; TEMPERATURE; TRANSCRIPTION, GENETIC;

CRICETINAE; CRICETULUS GRISEUS;

EID: 0037420754     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10566     Document Type: Article

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