Genetic engineering of mesenchymal stem cells by non-viral gene delivery

Clinical Hemorheology and Microcirculation

Volumn 58, Issue 1, 2014, Pages 19-48

  Wang, Weiwei ^a   Xu, Xun ^a   Li, Zhengdong ^a   Lendlein, Andreas ^a,b   Ma, Nan ^a,b  

^a FREIE UNIVERSITÄT BERLIN   (Germany)

^b Helmholtz Virtual Institute Multifunctional Biomaterials for Medicine   (Germany)

Author keywords

lipoplex; Mesenchymal stem cells; non viral gene delivery; polyplex; regenerative medicine; transfection

Indexed keywords

CATION; CATIONIC LIPID; CATIONIC POLYMER; INORGANIC COMPOUND; NANOPARTICLE; NUCLEIC ACID; POLYMER; UNCLASSIFIED DRUG; DNA; MESSENGER RNA; SMALL INTERFERING RNA;

ADIPOGENESIS; ANGIOGENESIS; ARTICLE; BONE DEVELOPMENT; CELL DIFFERENTIATION; CELL GROWTH; CELL ISOLATION; CELL MIGRATION; CELL SURVIVAL; CHEMICAL PROCEDURES; CHONDROGENESIS; CULTURE MEDIUM; CULTURE TECHNIQUE; DNA MODIFICATION; DRUG DELIVERY SYSTEM; ELECTROPORATION; GENE TARGETING; GENETIC ENGINEERING; GENETIC TRANSFECTION; HUMAN; IMMUNOGENICITY; MESENCHYMAL STEM CELL; MICROINJECTION; NONVIRAL GENE DELIVERY SYSTEM; REGENERATIVE MEDICINE; SURFACE PROPERTY; TISSUE ENGINEERING; VIRAL GENE DELIVERY SYSTEM; ANIMAL; BONE MARROW CELL; CELL LINEAGE; CHEMISTRY; CYTOLOGY; GENE TRANSFER; MESENCHYMAL STROMA CELL; METABOLISM; NANOTECHNOLOGY; PHASE CONTRAST MICROSCOPY; PROCEDURES;

ANIMALS; BONE MARROW CELLS; CELL DIFFERENTIATION; CELL LINEAGE; DNA; ELECTROPORATION; GENE TRANSFER TECHNIQUES; GENETIC ENGINEERING; HUMANS; MESENCHYMAL STROMAL CELLS; MICROSCOPY, PHASE-CONTRAST; NANOPARTICLES; NANOTECHNOLOGY; RNA, MESSENGER; RNA, SMALL INTERFERING; TISSUE ENGINEERING; TRANSFECTION;

EID: 84908229897     PISSN: 13860291     EISSN: 18758622     Source Type: Journal    
DOI: 10.3233/CH-141883     Document Type: Article

References (195)

1. H.H. Ahn, J.H. Lee, K.S. Kim, J.Y. Lee, M.S. Kim, G. Khang, I.W. Lee and H.B. Lee, Polyethyleneimine-mediated gene delivery into human adipose derived stem cells, Biomaterials 29 (2008), 2415-2422
2. A. Akinc, M. Thomas, A.M. Klibanov and R. Langer, Exploring polyethylenimine-mediated DNA transfection and the proton sponge hypothesis, Journal of Gene Medicine 7 (2005), 657-663
3. M. Aluigi, M. Fogli, A. Curti, A. Isidori, E. Gruppioni, C. Chiodoni, M.P. Colombo, P. Versura, A. D'Errico-Grigioni, E. Ferri, M. Baccarani and R.M. Lemoli, Nucleofection is an efficient nonviral transfection technique for human bone marrow-derived mesenchymal stem cells, Stem Cells 24 (2006), 454-461
4. F. Andre and L.M. Mir, DNA electrotransfer its principles and an updated review of its therapeutic applications, Gene Therapy 11 (2004), S33-S42
5. F. Arnold and D.C. West, Angiogenesis in Wound-Healing, Pharmacology & Therapeutics 52 (1991), 407-422
6. H. Aslan, Y. Zilberman, V. Arbeli, D. Sheyn, Y. Matan, M. Liebergall, J.Z. Li, G.A. Helm, D. Gazit and Z. Gazit, Nucleofection-based ex vivo nonviral gene delivery to human stem cells as a platform for tissue regeneration, Tissue Engineering 12 (2006), 877-889
7. L. Attisano and J.L. Wrana, Signal transduction by the TGF-beta superfamily, Science 296 (2002), 1646-1647
8. T. Azzam and A.J. Domb, Current developments in gene transfection agents, Curr Drug Deliv 1 (2004), 165-193
9. J.C. Babister, R.S. Tare, D.W. Green, S. Inglis, S. Mann and R.O.C. Oreffo, Genetic manipulation of human mesenchymal progenitors to promote chondrogenesis using "bead-in-bead" polysaccharide capsules, Biomaterials 29 (2008), 58-65
10. R. Bastos, N. Pante and B. Burke, Nuclear pore complex proteins, Int Rev Cytol 162 (1995), 257-302
11. D.J. Bharali, I. Klejbor, E.K. Stachowiak, P. Dutta, I. Roy, N. Kaur, E.J. Bergey, P.N. Prasad and M.K. Stachowiak, Organically modified silica nanoparticles: A nonviral vector for in vivo gene delivery and expression in the brain, Proceedings of the National Academy of Sciences of the United States of America 102 (2005), 11539-11544
12. J.S. Blum, M.A. Barry, A.G. Mikos and J.A. Jansen, In vivo evaluation of gene therapy vectors in ex vivo-derived marrow stromal cells for bone regeneration in a rat critical-size calvarial defect model, Human Gene Therapy 14 (2003), 1689-1701
13. C. Bolliet, M.C. Bohn and M. Spector, Non-viral delivery of the gene for glial cell line-derived neurotrophic factor to mesenchymal stem cells in vitro via a collagen scaffold, Tissue Engineering Part C-Methods 14 (2008), 207-219
14. A.C. Boquest, A. Shahdadfar, K. Fronsdal, O. Sigurjonsson, S.H. Tunheim, P. Collas and J.E. Brinchmann, Isolation and transcription profiling of purified uncultured human stromal stem cells: Alteration of gene expression after in vitro cell culture, Molecular Biology of the Cell 16 (2005), 1131-1141
15. P. Bosch, D.S. Musgrave, J.Y. Lee, J. Cummins, F. Shuler, S.C. Ghivizzani, C. Evans, P.D. Robbins and J. Huard, Osteoprogenitor cells within skeletal muscle, Journal of Orthopaedic Research 18 (2000), 933-944
16. H. Boulaiz, J.A. Marchal, J. Prados, C. Melguizo and A. Aranega, Non-viral and viral vectors for gene therapy, Cellular and Molecular Biology 51 (2005), 3-22
17. O. Boussif, F. Lezoualch, M.A. Zanta, M.D. Mergny, D. Scherman, B. Demeneix and J.P. Behr, A Versatile Vector for Gene and Oligonucleotide Transfer into Cells in Culture and in-Vivo - Polyethylenimine, Proceedings of the National Academy of Sciences of the United States of America 92 (1995), 7297-7301
18. M. Breitbach, T. Bostani, W. Roell, Y. Xia, O. Dewald, J.M. Nygren, J.W.U. Fries, K. Tiemann, H. Bohlen, J. Hescheler, A. Welz, W. Bloch, S.E.W. Jacobsen and B.K. Fleischmann, Potential risks of bone marrow cell transplantation into infarcted hearts, Blood 110 (2007), 1362-1369
19. C.T. Brighton, D.G. Lorich, R. Kupcha, T.M. Reilly, A.R. Jones and R.A.Woodbury, The pericyte as a possible osteoblast progenitor-cell, Clinical Orthopaedics and Related Research 275 (1992), 287-299
20. S. Brunner, E. Furtbauer, T. Sauer, M. Kursa and E. Wagner, Overcoming the nuclear barrier: Cell cycle independent nonviral gene transfer with linear polyethylenimine or electroporation, Molecular Therapy 5 (2002), 80-86
21. S. Brunner, T. Sauer, S. Carotta, M. Cotten, M. Saltik and E.Wagner, Cell cycle dependence of gene transfer by lipoplex polyplex and recombinant adenovirus, Gene Therapy 7 (2000), 401-407
22. V.R. Burst, M. Gillis, F. Putsch, R. Herzog, J.H. Fischer, P. Heid, J. Muller-Ehmsen, K. Schenk, J.W.U. Fries, C.A. Baldamus and T. Benzing, Poor cell survival limits the beneficial impact of mesenchymal stem cell transplantation on acute kidney injury, Nephron Experimental Nephrology 114 (2010), E107-E116
23. P. Campeau, P. Chapdelaine, S. Seigneurin-Venin, B. Massie and J.P. Tremblay, Transfection of large plasmids in primary human myoblasts, Gene Therapy 8 (2001), 1387-1394
24. P.J. Canatella and M.R. Prausnitz, Prediction and optimization of gene transfection and drug delivery by electroporation, Gene Therapy 8 (2001), 1464-1469
25. S. Chesnoy and L. Huang, Structure and function of lipid-DNA complexes for gene delivery, Annual Review of Biophysics and Biomolecular Structure 29 (2000), 27-47
26. C. Chu and H.Kong, Interplay of cell adhesion matrix stiffness and cell type for non-viral gene delivery, Acta Biomaterialia 8 (2012), 2612-2619
27. B.A. Clements, V. Incani, C. Kucharski, A. Lavasanifar, B. Ritchie and H. Uludag, A comparative evaluation of poly-Llysine- palmitic acid and Lipofectamine (TM) 2000 for plasmid delivery to bone marrow stromal cells, Biomaterials 28 (2007), 4693-4704
28. B.A. Clements, J. Bai, C.Kucharski, L.L. Farrell, A. Lavasanifar, B. Ritchie, A. Ghahary and H. Uludag, RGDconjugation to polyethyleneimine does not improve DNA delivery to bone marrow stromal cells, Biomacromolecules 7 (2006), 1481-1488
29. K. Corsi, F. Chellat, L. Yahia and J.C. Fernandes, Mesenchymal stem cells, MG63 and HEK293 transfection using chitosan-DNA nanoparticles, Biomaterials 24 (2003), 1255-1264
30. C.M. Curtin, G.M. Cunniffe, F.G. Lyons, K. Bessho, G.R. Dickson, G.P. Duffy and F.J. O'Brien, Innovative collagen nanohydroxyapatite scaffolds offer a highly efficient non-viral gene delivery platform for stem cell-mediated bone formation, Advanced Materials, 24 (2012), 749-754
31. H.L. Davis, R.G. Whalen and B.A. Demeneix, Direct gene-transfer into skeletal-muscle in vivo - factors affecting efficiency of transfer and stability of expression, Human Gene Therapy 4 (1993), 151-159
32. C. De Bari, F. Dell'Accio and F.P. Luyten, Human periosteum-derived cells maintain phenotypic stability and chondrogenic potential throughout expansion regardless of donor age, Arthritis and Rheumatism 44 (2001), 85-95
33. C. De Bari, F. Dell'Accio, P. Tylzanowski and F.P. Luyten, Multipotent mesenchymal stem cells from adult human synovial membrane, Arthritis and Rheumatism 44 (2001), 1928-1942
34. L. De Laporte and L.D. Shea, Matrices and scaffolds for DNA delivery in tissue engineering, Advanced Drug Delivery Reviews 59 (2007), 292-307
35. L. De Laporte, J.C. Rea and L.D. Shea, Design of modular non-viral gene therapy vectors, Biomaterials 27 (2006), 947-954
36. D.A. Dean, D.D. Strong and W.E. Zimmer, Nuclear entry of nonviral vectors, Gene Therapy 12 (2005), 881-890
37. A. Dhaliwal, M. Maldonado, Z. Han and T. Segura, Differential uptake of DNA-poly(ethylenimine) polyplexes in cells cultured on collagen and fibronectin surfaces, Acta Biomaterialia 6 (2010), 3436-3447
38. A. Dhaliwal, J. Lam, M. Maldonado, C. Lin and T. Segura, Extracellular matrix modulates non-viral gene transfer to mouse mesenchymal stem cells, Soft Matter 8 (2012), 1451-1459
39. A.C. Durieux, R. Bonnefoy, T. Busso and D. Freyssenet, In vivo gene electrotransfer into skeletal muscle: Effects of plasmid DNA on the occurrence and extent of muscle damage, Journal of Gene Medicine 6 (2004), 809-816
40. M. Endoh, N. Koibuchi, M. Sato, R. Morishita, T. Kanzaki, Y. Murata and Y. Kaneda, Fetal gene transfer by intrauterine injection with microbubble-enhanced ultrasound, Molecular Therapy 5 (2002), 501-508
41. K. Ewert, A. Ahmad, H.M. Evans, H.W. Schmidt and C.R. Safinya, Efficient synthesis and cell-transfection properties of a new multivalent cationic lipid for nonviral gene delivery, Journal of Medicinal Chemistry 45 (2002), 5023-5029
42. B.S. Fan and J.Y. Lou, Enhancement of angiogenic effect of co-transfection human NGF and VEGF genes in rat bone marrow mesenchymal stem cells, Gene 485 (2011), 167-171
43. L.L. Farrell, J. Pepin, C. Kucharski, X.Y. Lin, Z.H. Xu and H. Uludag, A comparison of the effectiveness of cationic polymers poly-L-lysine (PLL) and polyethylenimine (PEI) for non-viral delivery of plasmidDNAto bone marrowstromal cells (BMSC), European Journal of Pharmaceutics and Biopharmaceutics 65 (2007), 388-397
44. J.H. Felgner, R.Kumar, C.N. Sridhar, C.J. Wheeler, Y.J. Tsai, R. Border, P. Ramsey, M. Martin and P.L. Felgner, Enhanced gene delivery, mechanism studies with a novel series of cationic lipid formulations, Journal of Biological Chemistry 269 (1994), 2550-2561
45. P.L. Felgner, T.R. Gadek, M. Holm, R. Roman, H.W. Chan, M. Wenz, J.P. Northrop, G.M. Ringold and M. Danielsen, Lipofection: A highly efficient, lipid-mediated DNA-transfection procedure, Proc Natl Acad Sci U S A 84 (1987), 7413-7417
46. E. Ferreira, E. Potier, D. Logeart-Avramoglou, S. Salomskaite-Davalgiene, L.M. Mir and H. Petite, Optimization of a gene electrotransfer method for mesenchymal stem cell transfection, Gene Therapy 15 (2008), 537-544
47. J. Folkman, How is blood-vessel growth regulated in normal and neoplastic tissue - gha clowes memorial award lecture, Cancer Research 46 (1986), 467-473
48. A.J. Friedenstein, R.K. Chailakhyan, N.V. Latsinik, A.F. Panasyuk and I.V. Keiliss-Borok, Stromal cells responsible for transferring the microenvironment of the hemopoietic tissues. Cloning in vitro and retransplantation in vivo, Transplantation 17 (1974), 331-340
49. H.H. Fu, Y.H. Hu, T. McNelis and J.O. Hollinger, Acalcium phosphate-based gene delivery system, Journal of Biomedical Materials Research Part A 74 (2005), 40-48
50. D. Furlani, W.Z. Li, E. Pittermann, C. Klopsch, L.Wang, A. Knopp, P. Jungebluth, E. Thedinga, C. Havenstein, I.Westien, M. Ugurlucan, R.K. Li, N. Ma and G. Steinhoff, A transformed cell population derived from cultured mesenchymal stem cells has no functional effect after transplantation into the injured heart, Cell Transplantation 18 (2009), 319-331
51. E. Galanis, E.M. Hersh, A.T. Stopeck, R. Gonzalez, P. Burch, C. Spier, E.T. Akporiaye, J.J. Rinehart, J. Edmonson, R.E. Sobol, C. Forscher, V.K. Sondak, B.D. Lewis, E.C. Unger, M. O'Driscoll, L. Selk and J. Rubin, Immunotherapy of advanced malignancy by direct gene transfer of an interleukin-2DNA/DMRIE/DOPElipid complex: Phase I/II experience, Journal of Clinical Oncology 17 (1999), 3313-3323
52. K. Gao, Y. Lu, L. Wei, X. Lu, S. Li, L. Wan, Y. Li and J. Cheng, Immortalization of mesenchymal stem cells from bone marrowof rhesus monkey by transfection with human telomerase reverse transcriptase gene, Transplantation Proceedings 40 (2008), 634-637
53. L.Z. Gao, L. Nie, T.H. Wang, Y.J. Qin, Z.X. Guo, D.L. Yang and X.Y. Yan, Carbon nanotube delivery of the GFP gene into mammalian cells, Chembiochem 7 (2006), 239-242
54. H. Gissel and T. Clausen, Excitation-induced Ca2+ influx and skeletal muscle cell damage, Acta Physiologica Scandinavica 171 (2001), 327-334
55. O. Gresch, F.B. Engel, D. Nesic, T.T. Tran, H.M. England, E.S. Hickman, I. Korner, L. Gan, S. Chen, S. Castro-Obregon, R. Hammermann, J. Wolf, H. Muller-Hartmann, M. Nix, G. Siebenkotten, G. Kraus and K. Lun, New non-viral method for gene transfer into primary cells, Methods 33 (2004), 151-163
56. S.T. Guo, Y.Y. Huang, Q.A. Jiang, Y. Sun, L.D. Deng, Z.C. Liang, Q.A. Du, J.F. Xing, Y.L. Zhao, P.C. Wang, A.J. Dong and X.J. Liang, Enhanced gene delivery and siRNA silencing by gold nanoparticles coated with charge-reversal polyelectrolyte, ACS Nano 4 (2010), 5505-5511
57. S.J. Gwak and B.S. Kim, Poly(lactic-co-glycolic acid) nanosphere as a vehicle for gene delivery to human cord bloodderived mesenchymal stem cells: Comparison with polyethylenimine, Biotechnology Letters 30 (2008), 1177-1182
58. H. Haleem-Smith, A. Derfoul, C. Okafor, R. Tuli, D. Olsen, D.J. Hall and R.S. Tuan, Optimization of high-efficiency transfection of adult human mesenchymal stem cells in vitro, Molecular Biotechnology 30 (2005), 9-19
59. A. Hamm, N. Krott, I. Breibach, R. Blindt and A.K. Bosserhoff, Efficient transfection method for primary cells, Tissue Engineering 8 (2002), 235-245
60. S.W. Han, C. Nakamura, N. Kotobuki, I. Obataya, H. Ohgushi, T. Nagamune and J. Miyake, High-efficiency DNA injection into a single human mesenchymal stem cell using a nanoneedle and atomic force microscopy, Nanomedicine- Nanotechnology Biology and Medicine 4 (2008), 215-225
61. O. Hauger, E.E. Frost, R. Van Heeswijk, C. Deminiere, R. Xue, Y. Delmas, C. Combe, C.T.W. Moonen, N. Grenier and J.W.M. Bulte, MR evaluation of the glomerular homing of magnetically labeled mesenchymal stem cells in a rat model of nephropathy, Radiology 238 (2006), 200-210
62. C.X. He, N. Li, Y.L. Hu, X.M. Zhu, H.J. Li, M. Han, P.H. Miao, Z.J. Hu, G. Wang, W.Q. Liang, Y. Tabata and J.Q. Gao, Effective gene delivery to mesenchymal stem cells based on the reverse transfection and three-dimensional cell culture system, Pharm Res 28 (2011), 1577-1590
63. C.H. Heldin, K. Miyazono and P. tenDijke, TGF-beta signalling from cell membrane to nucleus through SMAD proteins, Nature 390 (1997), 465-471
64. M. Hoare, U. Greiser, S. Schu, K. Mashayekhi, E. Aydogan, M. Murphy, F. Barry, T. Ritter and T. O'Brien, Enhanced lipoplex-mediated gene expression in mesenchymal stem cells using reiterated nuclear localization sequence peptides, Journal of Gene Medicine 12 (2010), 207-218
65. C.P. Hodgkinson, J.A. Gomez, M. Mirotsou and V.J. Dzau, Genetic engineering of mesenchymal stem cells and its application in human disease therapy, Human Gene Therapy 21 (2010), 1513-1526
66. J. Hoelters, M. Ciccarella, M. Drechsel, C. Geissler, H. Gulkan, W. Bocker, M. Schieker, M. Jochum and P. Neth, Nonviral genetic modification mediates effective transgene expression and functional RNA interference in human mesenchymal stem cells, Journal of Gene Medicine 7 (2005), 718-728
67. A.M. Hoffman, J.A. Paxson, M.R. Mazan, A.M. Davis, S. Tyagi, S. Murthy and E.P. Ingenito, Lung-derived mesenchymal stromal cell post-transplantation survival, persistence, paracrine expression, and repair of elastase-injured lung, Stem Cells and Development 20 (2011), 1779-1792
68. J.H. Hong, E.S. Hwang, M.T. McManus, A. Amsterdam, Y. Tian, R. Kalmukova, E. Mueller, T. Benjamin, B.M. Spiegelman, P.A. Sharp, N. Hopkins and M.B. Yaffe, TAZ, a transcriptional modulator of mesenchymal stem cell differentiation, Science 309 (2005), 1074-1078
69. H. Hosseinkhani, Y. Inatsugu, Y. Hiraoka, S. Inoue, H. Shimokawa and Y. Tabata, Impregnation of plasmid DNA into three-dimensional scaffolds and medium perfusion enhance in vitro DNA expression of mesenchymal stem cells, Tissue Engineering 11 (2005), 1459-1475
70. H. Hosseinkhani, M. Hosseinkhani, N.P. Gabrielson, D.W. Pack, A. Khademhosseini and H. Kobayashi, DNA nanoparticles encapsulated in 3D tissue-engineered scaffolds enhance osteogenic differentiation of mesenchymal stem cells, Journal of Biomedical Materials Research Part A 85 (2008), 47-60
71. H. Hosseinkhani, T. Azzam, H. Kobayashi, Y. Hiraoka, H. Shimokawa, A.J. Domb and Y. Tabata, Combination of 3D tissue engineered scaffold and non-viral gene carrier enhance in vitro DNA expression of mesenchymal stem cells, Biomaterials 27 (2006), 4269-4278
72. Y. Hu, K.Y. Cai, Z. Luo, R. Zhang, L. Yang, L.H. Deng and K.D. Jandt, Surface mediated in situ differentiation of mesenchymal stem cells on gene-functionalized titanium films fabricated by layer-by-layer technique, Biomaterials 30 (2009), 3626-3635
73. Y.C. Huang, D. Kaigler, K.G. Rice, P.H. Krebsbach and D.J. Mooney, Combined angiogenic and osteogenic factor delivery enhances bone marrow stromal cell-driven bone regeneration, Journal of Bone and Mineral Research 20 (2005), 848-857
74. S.W. Hui, M. Langner, Y.L. Zhao, P. Ross, E. Hurley and K. Chan, The role of helper lipids in cationic liposome-mediated gene transfer, Biophysical Journal 71 (1996), 590-599
75. P.S. in 'tAnker, S.A. Scherjon, C. Kleijburg-van Der Keur, W.A. Noort, F.H.J. Claas, R.Willemze, W.E. Fibbe and H.H.H. Kanhai, Amniotic fluid as a novel source of mesenchymal stem cells for therapeutic transplantation, Blood 102 (2003), 1548-1549
76. V. Incani, E. Tunis, B.A. Clements, C. Olson, C. Kucharski, A. Lavasanifar and H. Uludag, Palmitic acid substitution on cationic polymers for effective delivery of plasmid DNA to bone marrow stromal cells, Journal of Biomedical Materials Research Part A 81 (2007), 493-504
77. L. Jeng, B.R. Olsen and M. Spector, Engineering, endostatin-producing, cartilaginous constructs for cartilage repair using nonviral transfection of chondrocyte-seeded and mesenchymal-stem-cell-seeded collagen scaffolds, Tissue Engineering Part A 16 (2010), 3011-3021
78. J. Jo, A. Okazaki, K. Nagane, M. Yamamoto and Y. Tabata, Preparation of cationized polysaccharides as gene transfection carrier for bone marrow-derived mesenchymal stem cells, Journal of Biomaterials Science-Polymer Edition 21 (2010), 185-204
79. J.I. Jo, N. Nagaya, Y. Miyahara, M. Kataoka, M. Harada-Shiba, K. Kangawa and Y. Tabata, Transplantation of genetically engineered mesenchymal stem cells improves cardiac function in rats with myocardial infarction: Benefit of a novel nonviral vector, cationized dextran, Tissue Engineering 13 (2007), 313-322
80. M.R. Jung, I.K. Shim, E.S. Kim, Y.J. Park, Y.I. Yang, S.K. Lee and S.J. Lee, Controlled release of cell-permeable gene complex from poly(L-lactide) scaffold for enhanced stem cell tissue engineering, Journal of Controlled Release 152 (2011), 294-302
81. S.W. Kang, H.J. Do, I.B. Han, D.A. Shin, H.O. Kim, J.H. Kim and S.H. Lee, Increase of chondrogenic potentials in adipose-derived stromal cells by co-delivery of type I and type II TGFbeta receptors encoding bicistronic vector system, J Control Release 160 (2012), 577-582
82. T.A. Karlsen, P. Mirtaheri, A. Shandadfar, Y. Floisand and J.E. Brinchmann, Effect of three-dimensional culture and incubator gas concentration on phenotype and differentiation capability of human mesenchymal stem cells, Journal of Cellular Biochemistry 112 (2011), 684-693
83. T.J.Kean, L. Duesler, R.G.Young, A. Dadabayev, A. Olenyik, M. Penn, J.Wagner, D.J. Fink, A.I. Caplan and J.E. Dennis, Development of a peptide-targeted, myocardial ischemia-homing, mesenchymal stem cell, Journal of Drug Targeting 20 (2012), 23-32
84. Y. Kido, J. Jo and Y. Tabata, A gene transfection for rat mesenchymal stromal cells in biodegradable gelatin scaffolds containing cationized polysaccharides, Biomaterials 32 (2011), 919-925
85. H.J. Kim, J.F. Greenleaf, R.R. Kinnick, J.T. Bronk and M.E. Bolander, Ultrasound-mediated transfection of mammalian cells, Human Gene Therapy 7 (1996), 1339-1346
86. J.H. Kim, K.H. Shin, T.Z. Li and H. Suh, Potential of nucleofected human MSCs for insulin secretion, Journal of Tissue Engineering and Regenerative Medicine 5 (2011), 761-769
87. J.H. Kim, J.S. Park, H.N. Yang, D.G.Woo, S.Y. Jeon, H.J. Do, H.Y. Lim, J.M. Kim and K.H. Park, The use of biodegradable PLGA nanoparticles to mediate SOX9 gene delivery in human mesenchymal stem cells (hMSCs) and induce chondrogenesis, Biomaterials 32 (2011), 268-278
88. N.Y. Kim, Y.B. Choi, C.I. Kang, H.H. Kim, J.M. Yang and S. Shin, An hydrophobically modified arginine peptide vector system effectively delivers DNA into human mesenchymal stem cells and maintains transgene expression with differentiation, Journal of Gene Medicine 12 (2010), 779-789
89. W.J. King, N.A. Kouris, S. Choi, B.M. Ogle andW.L. Murphy, Environmental parameters influence non-viral transfection of human mesenchymal stem cells for tissue engineering applications, Cell Tissue Res 347 (2012), 689-699
90. I. Koltover, T. Salditt, J.O. Radler and C.R. Safinya, An inverted hexagonal phase of cationic liposome-DNA complexes related to DNA release and delivery, Science 281 (1998), 78-81
91. K. Kunath, T. Merdan, O. Hegener, H. Haberlein and T. Kissel, Integrin targeting using RGD-PEI conjugates for in vitro gene transfer, Journal of Gene Medicine 5 (2003), 588-599
92. M. Lavertu, S. Methot, N. Tran-Khanh and M.D. Buschmann, High efficiency gene transfer using chitosan/DNA nanoparticles with specific combinations of molecular weight and degree of deacetylation, Biomaterials 27 (2006), 4815-4824
93. D. Lechardeur, A.S. Verkman and G.L. Lukacs, Intracellular routing of plasmid DNA during non-viral gene transfer, Advanced Drug Delivery Reviews 57 (2005), 755-767
94. O.K. Lee, T.K. Kuo, W.M. Chen, K.D. Lee, S.L. Hsieh and T.H. Chen, Isolation of multipotent mesenchymal stem cells from umbilical cord blood, Blood 103 (2004), 1669-1675
95. S.J. Lee, S.W. Kang, H.J. Do, I. Han, D.A. Shin, J.H. Kim and S.H. Lee, Enhancement of bone regeneration by gene delivery of BMP2/Runx2 bicistronic vector into adipose-derived stromal cells, Biomaterials 31 (2010), 5652-5659
96. Y. Lei and T. Segura, DNA delivery from matrix metalloproteinase degradable poly(ethylene glycol) hydrogels to mouse cloned mesenchymal stem cells, Biomaterials 30 (2009), 254-265
97. W. Li, N. Ma, L.L. Ong, C. Nesselmann, C. Klopsch, Y. Ladilov, D. Furlani, C. Piechaczek, J.M. Moebius, K. Lutzow, A. Lendlein, C. Stamm, R.K. Li and G. Steinhoff, Bcl-2 engineered MSCs inhibited apoptosis and improved heart function, Stem Cells 25 (2007), 2118-2127
98. W.J. Li, F. Nicol and F.C. Szoka, GALA: A designed synthetic pH-responsive amphipathic peptide with applications in drug and gene delivery, Advanced Drug Delivery Reviews 56 (2004), 967-985
99. W.Z. Li, N. Ma, L.L. Ong, A. Kaminski, C. Skrabal, M. Ugurlucan, P. Lorenz, H.H. Gatzen, K. Lutzow, A. Lendlein, B.M. Putzer, R.K. Li and G. Steinhoff, Enhanced thoracic gene delivery by magnetic nanobead-mediated vector, Journal of Gene Medicine 10 (2008), 897-909
100. W.Z. Li, C. Nesselmann, Z.H. Zhou, L.L. Ong, F. Ori, G.P. Tang, A. Kaminski, K. Lutzow, A. Lendlein, A. Liebold, C. Stamm, J. Wang, G. Steinhoff and N. Ma, Gene delivery to the heart by magnetic nanobeads, Journal of Magnetism and Magnetic Materials 311 (2007), 336-341
101. J.Y. Lim, S.H. Park, C.H. Jeong, J.H. Oh, S.M. Kim, C.H. Ryu, S.A. Park, J.G. Ahn, W. Oh, S.S. Jeun and J.W. Chang, Microporation is a valuable transfection method for efficient gene delivery into human umbilical cord blood-derived mesenchymal stem cells, BMC Biotechnology 10 (2010), 38
102. F. Liu, H. Qi, L. Huang and D. Liu, Factors controlling the efficiency of cationic lipid-mediated transfection in vivo via intravenous administration, Gene Therapy 4 (1997), 517-523
103. M.R. Loebinger, A. Eddaoudi, D. Davies and S.M. Janes, Mesenchymal stem cell delivery of TRAIL can climinate metastatic cancer, Cancer Research 69 (2009), 4134-4142
104. J.P. Luzio, B.M. Mullock, P.R. Pryor, M.R. Lindsay, D.E. James and R.C. Piper, Relationship between endosomes and lysosomes, Biochemical Society Transactions 29 (2001), 476-480
105. C. Madeira, S.C. Ribeiro, I.S.M. Pinheiro, S.A.M. Martins, P.Z. Andrade, C.L. da Silva and J.M.S. Cabral, Gene delivery to human bone marrow mesenchymal stem cells by microporation, Journal of Biotechnology 151 (2011), 130-136
106. C. Magin-Lachmann, G. Kotzamanis, L. D'Aiuto, H. Cooke, C. Huxley and E. Wagner, In vitro and in vivo delivery of intact BAC DNA - comparison of different methods, Journal of Gene Medicine 6 (2004), 195-209
107. B. Maitra, E. Szekely, K. Gjini, M.J. Laughlin, J. Dennis, S.E. Haynesworth and O. Koc, Human mesenchymal stem cells support unrelated donor hematopoietic stem cells and suppress T-cell activation, Bone Marrow Transplantation 33 (2004), 597-604
108. M. Mannisto, S. Ronkko, M. Matto, P. Honkakoski, M. Hyttinen, J. Pelkonen and A. Urtti, The role of cell cycle on polyplex-mediated gene transfer into a retinal pigment epithelial cell line, Journal of Gene Medicine 7 (2005), 466-476
109. P. Marschall, N. Malik and Z. Larin, Transfer of YACs up to 2.3 Mb intact into human cells with polyethylenimine, Gene Therapy 6 (1999), 1634-1637
110. J. Martin, K. Helm, P. Ruegg, M. Varella-Garcia, E. Burnham and S. Majka, Adult lung side population cells have mesenchymal stem cell potential, Cytotherapy 10 (2008), 140-151
111. J.M. McMahon, S. Conroy, M. Lyons, U. Greiser, C. O'Shea, P. Strappe, L. Howard, M. Murphy, F. Barry and T. O'Brien, Gene transfer into rat mesenchymal stem cells: A comparative study of viral and nonviral vectors, Stem Cells and Development 15 (2006), 87-96
112. L.K. Medina-Kauwe, J. Xie and S. Hamm-Alvarez, Intracellular trafficking of nonviral vectors, Gene Therapy 12 (2005), 1734-1751
113. P.L. Mok, S.K. Cheong, C.F. Leong, K.H. Chua and O. Ainoon, Extended and stable gene expression via nucleofection of MIDGE construct into adult human marrow mesenchymal stromal cells, Cytotechnology 64 (2012), 203-216
114. K.W.C. Monk and P.R. Cullis, Structural and fusogenic properties of cationic liposomes in the presence of plasmid DNA, Biophysical Journal 73 (1997), 2534-2545
115. I. Moret, J.E. Peris, V.M. Guillem, M. Benet, F. Revert, F. Dasi, A. Crespo and S.F. Alino, Stability of PEI-DNA and DOTAP-DNA complexes: Effect of alkaline pH, heparin and serum, Journal of Controlled Release 76 (2001), 169-181
116. I.K. Moutsatsos, G. Turgeman, S.H. Zhou, B.G. Kurkalli, G. Pelled, L. Tzur, P. Kelley, N. Stumm, S. Mi, R. Muller, Y. Zilberman and D. Gazit, Exogenously regulated stem cell-mediated gene therapy for bone regeneration, Molecular Therapy 3 (2001), 449-461
117. K. Nagane, J.I. Jo andY. Tabata, Promoted adipogenesis of rat mesenchymal stem cells by transfection of small interfering RNA complexed with a cationized dextran, Tissue Engineering Part A 16 (2010), 21-31
118. H. Nakahara, V.M. Goldberg and A.I. Caplan, Culture-expanded human periosteal-derived cells exhibit osteochondral potential in vivo, Journal of Orthopaedic Research 9 (1991), 465-476
119. S. Nakashima, Y. Matsuyama, A. Nitta, Y. Sakai and N. Ishiguro, Highly efficient transfection of human marrow stromal cells by nucleofection, Transplantation Proceedings 37 (2005), 2290-2292
120. A.J. Nauta andW.E. Fibbe, Immunomodulatory properties of mesenchymal stromal cells, Blood 110 (2007), 3499-3506
121. H.M. Nie and C.H. Wang, Fabrication and characterization of PLGA/HAp scaffolds for delivery of BMP-2 plasmid composite DNA, Journal of Controlled Release 120 (2007), 111-121
122. U. Noth, A.M. Osyczka, R. Tuli, N.J. Hickok, K.G. Danielson and R.S. Tuan, Multilineage mesenchymal differentiation potential of human trabecular bone-derived cells, Journal of Orthopaedic Research 20 (2002), 1060-1069
123. E.D. O'Cearbhaill, M. Murphy, F. Barry, P.E. McHugh and V. Barron, Behavior of human mesenchymal stem cells in fibrin-based vascular tissue engineering constructs, Annals of Biomedical Engineering 38 (2010), 649-657
124. A. Okazaki, J.I. Jo and Y. Tabata, A reverse transfection technology to genetically engineer adult stem cells, Tissue Engineering 13 (2007), 245-251
125. A.M. Osyczka, U. Noth, K.G. Danielson and R.S. Tuan, Different osteochondral potential of clonal cell lines derived from adult human trabecular bone, Reparative Medicine: Growing Tissues and Organs 961 (2002), 73-77
126. K. Otani, K. Yamahara, S. Ohnishi, H. Obata, S. Kitamura and N. Nagaya, Nonviral delivery of siRNA into mesenchymal stem cells by a combination of ultrasound and microbubbles, Journal of Controlled Release 133 (2009), 146-153
127. H.T. Pan, Q.X. Zheng and X.D. Guo, A novel synthetic peptide vector system for optimal gene delivery to bone marrow stromal cells, Journal of Peptide Science 13 (2007), 154-163
128. D. Pandita, J.L. Santos, J. Rodrigues, A.P. Pego, P.L. Granja and H. Tomas, Gene delivery into mesenchymal stem cells: A biomimetic approach using RGD nanoclusters based on poly(amidoamine) dendrimers, Biomacromolecules 12 (2011), 472-481
129. J.S. Park, K. Na, D.G. Woo, H.N. Yang, J.M. Kim, J.H. Kim, H.M. Chung and K.H. Park, Non-viral gene delivery of DNA polyplexed with nanoparticles transfected into human mesenchymal stem cells, Biomaterials 31 (2010), 124-132
130. J.S. Park, H.N. Yang, D.G. Woo, S.Y. Jeon, H.J. Do, H.Y. Lim, J.H. Kim and K.H. Park, Chondrogenesis of human mesenchymal stem cells mediated by the combination of SOX trio SOX5, 6, and 9 genes complexed with PEI-modified PLGA nanoparticles, Biomaterials 32 (2011), 3679-3688
131. A. Peister, J.A. Mellad, M. Wang, H.A. Tucker and D.J. Prockop, Stable transfection of MSCs by electroporation, Gene Therapy 11 (2004), 224-228
132. L. Peng, M. Liu, Y.N. Xue, S.W. Huang and R.X. Zhuo, Transfection and intracellular trafficking characteristics for poly(amidoamine)s with pendant primary amine in the delivery of plasmid DNA to bone marrow stromal cells, Biomaterials 30 (2009), 5825-5833
133. N. Pimpha, P. Sunintaboon, S. Inphonlek and Y. Tabata, Gene delivery efficacy of polyethyleneimine-introduced chitosan shell/poly(methyl methacrylate) core nanoparticles for rat mesenchymal stem cells, Journal of Biomaterials Science- Polymer Edition 21 (2010), 205-223
134. P. Pires, S. Simoes, S. Nir, R. Gaspar, N. Duzgunes and M.C.P. De Lima, Interaction of cationic liposomes and their DNA complexes with monocytic leukemia cells, Biochimica Et Biophysica Acta-Biomembranes 1418 (1999), 71-84
135. M.F. Pittenger, A.M. Mackay, S.C. Beck, R.K. Jaiswal, R. Douglas, J.D. Mosca, M.A. Moorman, D.W. Simonetti, S. Craig and D.R. Marshak, Multilineage potential of adult human mesenchymal stem cells, Science 284 (1999), 143-147
136. C. Plank, U. Schillinger, F. Scherer, C. Bergemann, J.S. Remy, F. Krotz, M. Anton, J. Lausier and J. Rosenecker, The magnetofection method: Using magnetic force to enhance gene delivery, Biological Chemistry 384 (2003), 737-747
137. N. Polisetty, A. Fatima, S.L. Madhira, V.S. Sangwan and G.K. Vemuganti, Mesenchymal cells from limbal stroma of human eye, Molecular Vision 14 (2008), 431-442
138. L. Prasmickaite, A. Hogset and K. Berg, The role of the cell cycle on the efficiency of photochemical gene transfection, Biochimica Et Biophysica Acta-General Subjects 1570 (2002), 210-218
139. Z. Pu, X. You, Q. Xu, F. Gao, X. Xie, H. Zhang and W. Jian'an, Protein expression of mesenchymal stem cells after transfection of pcDNA3.1(-)-hVEGF(1)(6)(5) by ultrasound-targeted microbubble destruction, J Biomed Biotechnol, 2011 (2011), 839653
140. A. Ramasubramanian, S. Shiigi, G.K. Lee and F. Yang, Non-viral delivery of inductive and suppressive genes to adiposederived stem cells for osteogenic differentiation, Pharmaceutical Research 28 (2011), 1328-1337
141. J. Rejman, G. Tavernier, N. Bavarsad, J. Demeester and S.C. De Smedt, mRNA transfection of cervical carcinoma and mesenchymal stem cells mediated by cationic carriers, J Control Release 147 (2010), 385-391
142. M.F. Ryser, F. Ugarte, S. Thieme, M. Bornhauser, A. Roesen-Wolff and S. Brenner, mRNA transfection of CXCR4-GFP fusion - Simply generated by PCR - Results in efficient migration of primary human mesenchymal stem cells, Tissue Engineering Part C-Methods 14 (2008), 179-184
143. J.L. Santos, E. Oramas, A.P. Pego, P.L. Granja and H. Tomas, Osteogenic differentiation of mesenchymal stem cells using PAMAM dendrimers as gene delivery vectors, Journal of Controlled Release 134 (2009), 141-148
144. J.L. Santos, D. Pandita, J. Rodrigues, A.P. Pego, P.L. Granja and H. Tomas, Non-viral gene delivery to mesenchymal stem cells: Methods, strategies and application in bone tissue engineering and regeneration, Current Gene Therapy 11 (2011), 46-57
145. J.L. Santos, D. Pandita, J. Rodrigues, A.P. Pego, P.L. Granja, G. Balian and H. Tomas, Receptor-mediated gene delivery using PAMAM dendrimers conjugated with peptides recognized by mesenchymal stem cells, Molecular Pharmaceutics 7 (2010), 763-774
146. J.L. Santos, H. Oliveira, D. Pandita, J. Rodrigues, A.P. Pego, P.L. Granja and H. Tomas, Functionalization of poly(amidoamine) dendrimers with hydrophobic chains for improved gene delivery in mesenchymal stem cells, Journal of Controlled Release 144 (2010), 55-64
147. A. Saraf, M.C. Hacker, B. Sitharaman, K.J. Grande-Allen, M.A. Barry and A.G. Mikos, Synthesis and conformational evaluation of a novel gene delivery vector for human mesenchymal stem cells, Biomacromolecules 9 (2008), 818-827
148. N.K. Satija, V.K. Singh, Y.K. Verma, P. Gupta, S. Sharma, F. Afrin, M. Sharma, P. Sharma, R.P. Tripathi and G.U. Gurudutta, Mesenchymal stem cell-based therapy: A new paradigm in regenerative medicine, Journal of Cellular and Molecular Medicine 13 (2009), 4385-4402
149. A. Shakhbazau, D. Shcharbin, N. Petyovka, N. Goncharova, I. Seviaryn, S.Kosmacheva, M. Bryszewska and M. Potapnev, Non-virally modified human mesenchymal stem cells produce ciliary neurotrophic factor in biodegradable fibrin-based 3D scaffolds, Journal of Pharmaceutical Sciences 101 (2012), 1546-1554
150. A. Shakhbazau, D. Shcharbin, I. Seviaryn, N. Goncharova, S. Kosmacheva, M. Potapnev, B. Gabara, M. Ionov and M. Bryszewska, Use of polyamidoamine dendrimers to engineer BDNF-producing human mesenchymal stem cells, Molecular Biology Reports 37 (2010), 2003-2008
151. J.J. Shi, X. Zhang, Y.B. Pi, J.X. Zhu, C.Y. Zhou and Y.F. Ao, Nanopolymers delivery of the bone morphogenetic protein-4 plasmid to mesenchymal stem cells promotes articular cartilage repair in vitro and in vivo, Journal of Nanomaterials, (2012), 236953
152. S. Simoes, V. Slepushkin, R. Gaspar, M.C.P. De Lima and N. Duzgunes, Gene delivery by negatively charged ternary complexes of DNA, cationic liposomes and transferrin or fusigenic peptides, Gene Therapy 5 (1998), 955-964
153. A. Stolzing, E. Jones, D. McGonagle and A. Scutt, Age-related changes in human bone marrow-derived mesenchymal stem cells: Consequences for cell therapies, Mechanisms of Ageing and Development 129 (2008), 163-173
154. J.Y. Sun, V. Anand-Jawa, S. Chatterjee and K.K.Wong, Immune responses to adeno-Associated virus and its recombinant vectors, Gene Ther 10 (2003), 964-976
155. X.D. Sun, L. Jeng, C. Bolliet, B.R. Olsen and M. Spector, Non-viral endostatin plasmid transfection of mesenchymal stem cells via collagen scaffolds, Biomaterials 30 (2009), 1222-1231
156. W.B. Tan, S. Jiang and Y. Zhang, Quantum-dot based nanoparticles for targeted silencing of HER2/neu gene via RNA interference, Biomaterials 28 (2007), 1565-1571
157. F.X. Tang and J.A. Hughes, Introduction of a disulfide bond into a cationic lipid enhances transgene expression of plasmid DNA, Biochemical and Biophysical Research Communications 242 (1998), 141-145
158. A.K.K. Teo and L. Vallier, Emerging use of stem cells in regenerative medicine, Biochemical Journal 428 (2010), 11-23
159. D.K. Thakor, Y.D. Teng, H. Obata, K. Nagane, S. Saito and Y. Tabata, Nontoxic, genetic engineering of mesenchymal stem cells using serum-compatible pullulan-spermine/DNA anioplexes, Tissue Engineering Part C-Methods 17 (2011), 131-144
160. E.G. Tierney, G.P. Duffy, A.J. Hibbitts, S.A. Cryan and F.J. O'Brien, The development of non-viral gene-Activated matrices for bone regeneration using polyethyleneimine (PEI) and collagen-based scaffolds, Journal of Controlled Release 158 (2012), 304-311
161. T.V. Tsulaia, N.L. Prokopishyn, A. Yao, N.D.V. Carsrud, M.C. Carou, D.B. Brown, B.R. Davis and J. Yannariello-Brown, Glass needle-mediated microinjection of macromolecules and transgenes into primary human mesenchymal stem cells, Journal of Biomedical Science 10 (2003), 328-336
162. E. Uchimura, S. Yamada, L. Uebersax, S. Fujita, M. Miyake and J. Miyake, Method for reverse transfection using gold colloid as a nano-scaffold, Journal of Bioscience and Bioengineering 103 (2007), 101-103
163. E. Uchimura, S. Yamada, T. Nomura, K. Matsumoto, S. Fujita, M. Miyake and J. Miyake, Reverse Transfection using antibodies against a cell surface antigen in mammalian adherent cell lines, Journal of Bioscience and Bioengineering 104 (2007), 152-155
164. V. Vacanti, E. Kong, G. Suzuki, K. Sato, J.M. Canty and T.C. Lee, Phenotypic changes of adult porcine mesenchymal stem cells induced by prolonged passaging in culture, Journal of Cellular Physiology 205 (2005), 194-201
165. S. Vanderbyl, G.N. MacDonald, S. Sidhu, L. Gung, A. Telenius, C. Perez and E. Perkins, Transfer and stable transgene expression of a mammalian artificial chromosome into bone marrow-derived human mesenchymal stem cells, Stem Cells 22 (2004), 324-333
166. J. Wallmichrath, G.B. Stark, U. Kneser, C. Andree, M. Voigt, R.E. Horch and D.J. Schaefer, Epidermal growth factor (EGF) transfection of human bone marrow stromal cells in bone tissue engineering, Journal of Cellular and Molecular Medicine 13 (2009), 2593-2601
167. M.Wang, W.W. Deng, M. Fu, X. Cao, Y.Yang, W.Y. Su, J.N.Yu and X.M. Xu, Efficient gene transfer into rat mesenchymal stem cells with cationized Lycium barbarum polysaccharides nanoparticles, Carbohydrate Polymers 86 (2011), 1509-1518
168. W.G.Wang, S.Q. Lou, X.D. Ju, K. Xia and J.H. Xia, In vitro chondrogenesis of human bone marrow-derived mesenchymal progenitor cells in monolayer culture: Activation by transfection with TGF-beta2, Tissue Cell 35 (2003), 69-77
169. W.W.Wang, W.Z. Li, N. Ma and G. Steinhoff, Non-viral gene delivery methods, Current Pharmaceutical Biotechnology 14 (2013), 46-60
170. W.W. Wang, N. Ma, K. Kratz, X. Xu, Z.D. Li, T. Roch, K. Bieback, F. Jung and A. Lendlein, The influence of polymer scaffolds on cellular behaviour of bone marrow derived human mesenchymal stem cells, Clinical Hemorheology and Microcirculation 52 (2012), 357-373
171. W.W.Wang, W.Z. Li, L.L. Ong, K. Lutzow, A. Lendlein, D. Furlani, R. Gabel, D.L. Kong, J.Wang, R.K. Li, G. Steinhoff and N. Ma, Localized and sustained SDF-1 gene release mediated by fibronectin films: A potential method for recruiting stem cells, International Journal of Artificial Organs 32 (2009), 141-149
172. W.W. Wang, W.Z. Li, L.L. Ong, D. Furlani, A. Kaminski, A. Liebold, K. Lutzow, A. Lendlein, J. Wang, R.K. Li, G. Steinhoff and N. Ma, Localized SDF-1alpha gene release mediated by collagen substrate induces CD117+ stem cells homing, Journal of Cellular and Molecular Medicine 14 (2010), 392-402
173. W.W. Wang, W.Z. Li, L.L. Ou, E. Flick, P. Mark, C. Nesselmann, C.A. Lux, H.H. Gatzen, A. Kaminski, A. Liebold, K. Luetzow, A. Lendlein, R.K. Li, G. Steinhoff and N. Ma, Polyethylenimine-mediated gene delivery into human bone marrow mesenchymal stem cells from patients, Journal of Cellular and Molecular Medicine 15 (2011), 1989-1998
174. A.K. Wege, K. Schardt, S. Schaefer, A. Kroemer, G. Brockhoff and E.M. Jung, High resolution ultrasound including elastography and contrast-enhanced ultrasound (CEUS) for early detection and characterization of liver lesions in the humanized tumor mouse model, Clin Hemorheol Microcirc 52 (2012), 93-106
175. L.L. Wei, K. Gao, P.Q. Liu, X.F. Lu, S.F. Li, J.Q. Cheng, Y.P. Li and Y.R. Lu, Mesenchymal stem cells from Chinese Guizhou minipig by hTERT gene transfection, Transplantation Proceedings 40 (2008), 547-550
176. S.R. Wente, Gatekeepers of the nucleus, Science 288 (2000), 1374-1377
177. C.J. Wheeler, P.L. Felgner, Y.J. Tsai, J. Marshall, L. Sukhu, S.G. Doh, J. Hartikka, J. Nietupski, M. Manthorpe, M. Nichols, M. Plewe, X. Liang, J. Norman, A. Smith and S.H. Cheng, A novel cationic lipid greatly enhances plasmid DNA delivery and expression in mouse lung, Proc Natl Acad Sci U S A 93 (1996), 11454-11459
178. J.M. Wiehe, P. Ponsaerts, M.T. Rojewski, J.M. Homann, J. Greiner, D. Kronawitter, H. Schrezenmeier, V. Hombach, M. Wiesneth, O. Zimmermann and J. Torzewski, mRNA-mediated gene delivery into human progenitor cells promotes highly efficient protein expression, Journal of Cellular and Molecular Medicine 11 (2007), 521-530
179. D.P. Wolf, J.A. Thomson, M.B. Zelinskiwooten and R.L. Stouffer, In vitro fertilization-embryo transfer in nonhumanprimates - the technique and its applications, Molecular Reproduction and Development 27 (1990), 261-280
180. H.C. Wu, T.W. Wang, M.C. Bohn, F.H. Lin and M. Spector, Novel magnetic, hydroxyapatite, nanoparticles as non-viral vectors for the glial cell line-derived neurotrophic factor gene, Advanced Functional Materials 20 (2010), 67-77
181. H. Xin, M. Kanehira, H. Mizuguchi, T. Hayakawa, T. Kikuchi, T. Nukiwa and Y. Saijo, Targeted delivery of CX3CL1 to multiple lung tumors by mesenchymal stem cells, Stem Cells 25 (2007), 1618-1626
182. Y.H. Xu and F.C. Szoka, Mechanism of DNA release from cationic liposome/DNA complexes used in cell transfection, Biochemistry 35 (1996), 5616-5623
183. F. Yang, J.J. Green, T. Dinio, L. Keung, S.W. Cho, H. Park, R. Langer and D.G. Anderson, Gene delivery to human adult and embryonic cell-derived stem cells using biodegradable nanoparticulate polymeric vectors, Gene Therapy 16 (2009), 533-546
184. H.N. Yang, J.S. Park, D.G. Woo, S.Y. Jeon, H.J. Do, H.Y. Lim, S.W. Kim, J.H. Kim and K.H. Park, Chondrogenesis of mesenchymal stem cells and dedifferentiated chondrocytes by transfection with SOX Trio genes, Biomaterials 32 (2011), 7695-7704
185. H.E. Young, T.A. Steele, R.A. Bray, J. Hudson, J.A. Floyd, K. Hawkins, K. Thomas, T. Austin, C. Edwards, J. Cuzzourt, M. Duenzl, P.A. Lucas and A.C. Black, Human reserve pluripotent mesenchymal stem cells are present in the connective tissues of skeletal muscle and dermis derived from fetal, adult, and geriatric donors, Anatomical Record 264 (2001), 51-62
186. J. Zabner, A.J. Fasbender, T. Moninger, K.A. Poellinger and M.J.Welsh, Cellular, molecular, barriers to gene-transfer by a cationic lipid, Journal of Biological Chemistry 270 (1995), 18997-19007
187. A.K. Zaiss and D.A. Muruve, Immune responses to adeno-Associated virus vectors, Current Gene Therapy 5 (2005), 323-331
188. J.M. Zeng, X.Wang and S.Wang, Self-Assembled ternary complexes of plasmidDNA, lowmolecular weight polyethylenimine and targeting peptide for nonviral gene delivery into neurons, Biomaterials 28 (2007), 1443-1451
189. C.Y. Zhan, J. Qian, L.L. Feng, G.R. Zhong, J.H. Zhu andW.Y. Lu, Cyclic RGD-poly(ethylene glycol)-polyethyleneimine is more suitable for glioblastoma targeting gene transfer in vivo, Journal of Drug Targeting 19 (2011), 573-581
190. S.G. Zhong, S.Y. Shu, Z.G.Wang, J. Luo, W.Y. Zhong, H.T. Ran, Y.Y. Zheng, Y.H. Yin and Z.Y. Ling, Enhanced homing of mesenchymal stem cells to the ischemic myocardium by ultrasound-targeted microbubble destruction, Ultrasonics 52 (2012), 281-286
191. J. Ziauddin and D.M. Sabatini, Microarrays of cells expressing defined cDNAs, Nature 411 (2001), 107-110
192. R. Ziv, Y. Steinhardt, G. Pelled, D. Gazit and B. Rubinsky, Micro-electroporation of mesenchymal stem cells with alternating electrical current pulses, Biomedical Microdevices 11 (2009), 95-101
193. N.J. Zuidam and Y. Barenholz, Electrostatic and structural properties of complexes involving plasmid DNA and cationic lipids commonly used for gene delivery, Biochimica Et Biophysica Acta-Biomembranes 1368 (1998), 115-128
194. P.A. Zuk, M. Zhu, H. Mizuno, J. Huang, J.W. Futrell, A.J. Katz, P. Benhaim, H.P. Lorenz and M.H. Hedrick, Multilineage cells from human adipose tissue: Implications for cell-based therapies, Tissue Engineering 7 (2001), 211-228
195. N.J. Zvaifler, L. Marinova-Mutafchieva, G. Adams, C.J. Edwards, J. Moss, J.A. Burger and R.N. Maini, Mesenchymal precursor cells in the blood of normal individuals, Arthritis Research 2 (2000), 477-488