Microporation is a valuable transfection method for efficient gene delivery into human umbilical cord blood-derived mesenchymal stem cells

BMC Biotechnology

Volumn 10, Issue , 2010, Pages

  Lim, Jung Y ^a   Park, Sun H ^a   Jeong, Chang H ^a   Oh, Ji H ^a   Kim, Seong M ^a   Ryu, Chung H ^a   Park, Soon A ^a   Ahn, Jae G ^a   Oh, Wonil ^c   Jeun, Sin Soo ^a,b   Chang, Jong W ^c  

^a The Catholic University of Korea   (South Korea)

^b The Catholic University of Korea   (South Korea)

^c MEDIPOST Co Ltd   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

ADULT STEM CELLS; CANCER CELLS; CELL DAMAGE; CLINICAL STUDY; ELECTROPORATION; ENHANCED GREEN FLUORESCENT PROTEIN; GENE DELIVERY; GENETIC MODIFICATIONS; HIGHER EFFICIENCY; HUMAN UMBILICAL CORD; IMMUNOPHENOTYPES; IN-VITRO; MALIGNANT GLIOMA; MESENCHYMAL STEM CELL; MIGRATION ABILITY; NONVIRAL; PLASMID DNA; PROLIFERATION ACTIVITY; REPORTER GENE; STEM CELL; STEM CELL THERAPY; THERAPEUTIC APPLICATION; THERAPEUTIC USE; TRANSFECTION METHODS; TRANSGENICS;

BLOOD; CELL CULTURE; FLOWCHARTING; GENE EXPRESSION; GENE THERAPY; GENE TRANSFER; NUCLEIC ACIDS;

STEM CELLS;

BRAIN DERIVED NEUROTROPHIC FACTOR; ENHANCED GREEN FLUORESCENT PROTEIN; LIPOSOME; PLASMID DNA; GREEN FLUORESCENT PROTEIN;

ANIMAL EXPERIMENT; ARTICLE; CANCER CELL; CELL ACTIVITY; CELL DAMAGE; CELL LINEAGE; CELL MIGRATION; CELL PROLIFERATION; CONTROLLED STUDY; ECTODERM; ELECTROPORATION; GENE DELIVERY SYSTEM; GENE EXPRESSION; GENETIC ENGINEERING; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IMMUNOPHENOTYPING; MALE; MESENCHYMAL STEM CELL; MESODERM; NERVE CELL DIFFERENTIATION; NONHUMAN; RAT; REPORTER GENE; UMBILICAL CORD BLOOD; ANIMAL; CELL CULTURE; CELL DIFFERENTIATION; CELL MOTION; CYTOLOGY; FETUS BLOOD; GENETICS; METABOLISM; METHODOLOGY; PLASMID; SPRAGUE DAWLEY RAT;

ANIMALS; BRAIN-DERIVED NEUROTROPHIC FACTOR; CELL DIFFERENTIATION; CELL MOVEMENT; CELL PROLIFERATION; CELLS, CULTURED; ELECTROPORATION; FETAL BLOOD; GENES, REPORTER; GREEN FLUORESCENT PROTEINS; HUMANS; MALE; MESENCHYMAL STEM CELLS; PLASMIDS; RATS; RATS, SPRAGUE-DAWLEY; TRANSFECTION;

EID: 77952072550     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-10-38     Document Type: Article

References (36)

1. Goodwin HS, Bicknese AR, Chien SN, Boqucki BD, Quinn CO, Wall DA. Multilineage differentiation activity by cells isolated from umbilical cord blood: expression of bone, fat, and neural markers. Biol Blood Marrow Transplant 2001, 7:581-588. 10.1053/bbmt.2001.v7.pm11760145, 11760145.
2. Pittenger MF, Mackay AM, Beck SC, Jaiswal PK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S, Marshak DR. Multilineage potential of adult human mesenchymal stem cells. Science 1999, 284:143-147. 10.1126/science.284.5411.143, 10102814.
3. Caplan AI. Mesenchymal stem cells. J Orthop Res 1991, 9:641-650. 10.1002/jor.1100090504, 1870029.
4. Parr AM, Tator CH, Keating A. Bone marrow-derived mesenchymal stromal cells for the repair of central nervous system injury. Bone Marrow Transplant 2007, 40:609-619. 10.1038/sj.bmt.1705757, 17603514.
5. Li Y, Chen J, Chen XG, Wang L, Gautam SC, Xu YX, Katakowski M, Zhang LJ, Lu M, Janakiraman N, Chopp M. Human marrow stromal cell therapy for stroke in rat: neurotrophins and functional recovery. Neurology 2002, 59:514-523.
6. Studeny M, Marini FC, Dembinski JL, Zompetta C, Cabreira-Hansen M, Bekele BN, Champin RE, Andreeff M. Mesenchymal stem cells: potential precursors for tumor stroma and targeted delivery vehicles for anticancer agents. J Natl Can Inst 2004, 96:1593-1603.
7. Studeny M, Marini FC, Champlin RE, Zompetta C, Fidler IJ, Andreeff M. Bone marrow derived mesenchymal stem cells as vehicles for interferon-b delivery into tumors. Cancer Res 2002, 62:3603-3608.
8. Le Blanc K, Tammik L, Sundberg B, Haynesworth SE, Ringden O. Mesenchymal stem cells inhibit and stimulate mixed lymphocyte cultures and mitogenic responses independently of the major histocompatibility complex. Scand J Immunol 2003, 57:11-20. 10.1046/j.1365-3083.2003.01176.x, 12542793.
9. Nakamizo A, Marini F, Amano T, Khan A, Studeny M, Gumin J, Chen J, Hentschel S, Vecil G, Dembinski J, Andreeff M, Lang FF. Human bone marrow-derived mesenchymal stem cells in the treatment of gliomas. Cancer Res 2005, 65:3307-3318.
10. Consiglio A, Gritti A, Dolcetta D, Follenzi A, Bordiqnon C, Gage FH, Vesconvi AL, Naldini L. Robust in vivo gene transfer into adult mammalian neural stem cells by lentiviral vectors. Proc Natl Acad Sci USA 2004, 101:14835-14840. 10.1073/pnas.0404180101, 522006, 15466696.
11. Park F, Ohashi K, Chiu W, Naldini L, Kay MA. Efficient lentiviral transduction of liver requires cell cycling in vivo. Nat Genet 2000, 24:49-52. 10.1038/71673, 10615126.
12. Naldini L, Blomer U, Gage FH, Trono D, Verma IM. Efficient transfer, integration, and sustained long-term expression of the transgene in adult rat brains injected with a lentiviral vector. Proc Natl Acad Sci USA 1996, 93:11382-11388. 10.1073/pnas.93.21.11382, 38066, 8876144.
13. Olmsted-Davis EA, Gugala Z, Gannon FH, Yotnda P, McAlhany RE, Lindsey RW, Davis AR. Use of a chimeric adenovirus vector enhances BMP2 production and bone formation. Hum Gene Ther 2002, 13:1337-1347. 10.1089/104303402760128568, 12162816.
14. Kushibiki T, Tabata Y. A new gene delivery system based on controlled release technology. Current Drug Delivery 2004, 1:153-163. 10.2174/1567201043479948, 16305381.
15. Hall KM, Horvath TL, Abonour R, Cornetta K, Srour EF. Decreased homing of retrovirally transduced human bone marrow CD34+ cells in the NOD/SCID mouse model. Exp Hematol 2006, 34:433-442. 10.1016/j.exphem.2005.12.014, 16569590.
16. Nishikawa M, Huang L. Nonviral vectors in the new millennium:delivery barriers in gene transfer. Hum Gene Ther 2001, 12:861-870. 10.1089/104303401750195836, 11387052.
17. Almofti MR, Harashima H, Shinohara Y, Almofti A, Li W, Kiwada H. Lipoplex size determines lipofection efficiency with or without serum. Mol Membr Biol 2003, 20:35-43. 10.1080/09687680210035104, 12745924.
18. Kubiniec RT, Liang H, Hui SW. Effect of pulse length and pulse strength on transfection by electroporation. BioTechniques 1990, 8:16-20.
19. Watanabe SY, Albsoul-Younes AM, Kawano T, Itoh H, Kaziro Y, Nakajima S, Nakajima Y. Calcium phosphate-mediated transfection of primary cultured brain neurons using GFP expression as a marker: application for single neuron electrophysiology. Neurosci Res 1999, 33:71-78. 10.1016/S0168-0102(98)00113-8, 10096474.
20. Teissie J, Golzio M, Rols MP. Mechanisms of cell membrane electropermeabilization: a minireview of our present knowledge. Biochim Biophys Acta 2005, 1724:270-280.
21. Mir LM. Therapeutic perspectives of in vivo cell electropermeabilization. Bioelectrochemistry 2001, 53:1-10. 10.1016/S0302-4598(00)00112-4, 11206915.
22. Mir LM, Bureau MF, Gehl J, Rangara R, Rouy D, Caillaud JM, Delaere P, Branellec D, Schwartz B, Scherman D. High-efficiency gene transfer into skeletal muscle mediated by electric pulses. Proc Natl Acad Sci USA 1999, 96:4262-4267. 10.1073/pnas.96.8.4262, 16320, 10200250.
23. Colleoni S, Donofrio G, Lagutina I, Duchi R, Galli C, Lazzari G. Establishment, differentiation, electroporation, viral transduction, and nuclear transfer of bovine and porcine mesenchymal stem cells. Cloning Stem Cells 2005, 7:154-165. 10.1089/clo.2005.7.154, 16176125.
24. Peister A, Mellad JA, Wang M, Tucker HA, Prockop DJ. Stable transfection of MSCs by electroporation. Gene Therapy 2004, 11:224-228. 10.1038/sj.gt.3302163, 14712307.
25. Huang EJ, Reichardt LF. Trk receptors: roles in neuronal signal transduction. Annu Rev Biochem 2003, 72:609-642. 10.1146/annurev.biochem.72.121801.161629, 12676795.
26. Ahmed S, Reynolds BA, Weiss S. BDNF enhances the differentiation but not the survival of CNS stem cell-derived neuronal precursors. J Neurosci 1995, 15:5765-5778.
27. Lim JY, Park SI, Kim SM, Oh JH, Jeong CH, Jun JA, Lee KS, Oh W, Lee JK, Jeun SS. Brain-derived neurotrophic factor stimulates the neural differentiation of human umbilical cord blood-derived mesenchymal stem cells and survival. J Neurosci Res 2008, 86:2168-2178. 10.1002/jnr.21669, 18438930.
28. Lu P, Jones LL, Tuszynski MH. BDNF-expressing marrow stromal cells support extensive axonal growth at sites of spinal cord injury. Exp Neurol 2005, 191:344-360. 10.1016/j.expneurol.2004.09.018, 15649491.
29. Kurozumi K, Nakamura K, Tamiya T, Kawano Y, Kobune M, Hirai S, Uchida H, Sasaki K, Ito Y, Kato K, Honmou O, Houkin K, Date I, Hamada H. BDNF gene-modified mesenchymal stem cells promote functional recovery and reduce infarct size in the rat middle cerebral artery occlusion model. Mol Ther 2004, 9:189-197. 10.1016/j.ymthe.2003.10.012, 14759803.
30. Tsuda H, Wada T, Ito Y, Uchida H, Dehari H, Nakamura K, Sasaki K, Kobune M, Yamashita T, Hamada H. Efficient BMP2 gene transfer and bone formation of mesenchymal stem cells by a fiber mutant adenoviral vector. Mol Ther 2003, 7:354-365. 10.1016/S1525-0016(02)00062-X, 12668131.
31. Gazit D, Turgeman G, Kelley P, Wang E, Jalenak M, Zilberman Y, Moutsatsos I. Engineered pluripotent mesenchymal cells integrate and differentiate in regenerating bone: a novel cell-mediated gene therapy. J Gene Med 1999, 1:121-133. 10.1002/(SICI)1521-2254(199903/04)1:2<121::AID-JGM26>3.0.CO;2-J, 10738576.
32. Wang YH, Ho ML, Chang JK, Chu HC, Lai SC, Wang GJ. Microporation is a valuable transfection method for gene expression in human adipose tissue-derived stem cells. Mol Ther 2009, 17:302-308. 10.1038/mt.2008.267, 2835049, 19066595.
33. Jeong JA, Gang EJ, Hong SH, Hwang SH, Kim SW, Yang IH, Ahn C, Han H, Kim H. Rapid neural differentiation of human cord blood-derived mesenchymal stem cells. Neuroreport 2004, 15:1731-1734. 10.1097/01.wnr.0000134846.79002.5c, 15257137.
34. Zigova T, Song S, Willing AE, Hudson JE, Newman MB, Saporta S, Sanchez-Ramos J, Sanberg PR. Human umbilical cord blood cells express neural antigens after transplantation into the developing rat brain. Cell Transplant 2002, 11:265-274.
35. Kuh SU, Cho YE, Yoon DH, Kim KN, Ha Y. Functional recovery after human umbilical cord blood cells transplantation with brain-derived neutrophic factor into the spinal cord injured rat. Acta Neurochir 2005, 147:985-992.
36. Yang SE, Ha CW, Jung M, Jin HJ, Lee M, Song H, Choi S, Oh W, Yang YS. Mesenchymal stem/progenitor cells developed in cultures from UC blood. Cytotherapy 2004, 6:476-486. 10.1080/14653240410005041, 15512914.