Stable transfection of MSCs by electroporation

Gene Therapy

Volumn 11, Issue 2, 2004, Pages 224-228

  Peister, A ^a   Mellad, J A ^a   Wang, M ^b   Tucker, H A ^a   Prockop, D J ^a  

^a TULANE UNIVERSITY HEALTH SCIENCES CENTER   (United States)

^b OSLO UNIVERSITY HOSPITAL   (Norway)

Author keywords

Human adult stem cells; Marrow stromal cells; Mesenchymal stem cells

Indexed keywords

ANTIBIOTIC G 418; CHLOROQUINE; DNA; EPIDERMAL GROWTH FACTOR RECEPTOR; GREEN FLUORESCENT PROTEIN; KANAMYCIN KINASE; PHOTOPROTEIN; PLASMID DNA; PLASTIC;

ADIPOCYTE; ANIMAL; ANIMAL CELL; ARTICLE; AUTOLOGOUS HEMATOPOIETIC STEM CELL TRANSPLANTATION; BONE MARROW CELL; CELL ADHESION; CELL COUNT; CELL CULTURE; CELL DIFFERENTIATION; CELL DIVISION; CELL ISOLATION; CELL LINE; CONTROLLED STUDY; CYTOLOGY; DNA INTEGRATION; ELECTROPORATION; FLOW CYTOMETRY; GENE EXPRESSION; GENE TARGETING; GENE THERAPY; GENETIC TRANSFECTION; GENETICS; HEMATOPOIETIC STEM CELL; HUMAN; HUMAN CELL; MALE; METHODOLOGY; NONHUMAN; OSTEOBLAST; PLASMID; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; RAT STRAIN; STROMA CELL; TRANSGENE;

ADIPOCYTES; ANIMALS; BONE MARROW CELLS; CELL DIFFERENTIATION; CELL DIVISION; CELL LINE; CHLOROQUINE; DNA; ELECTROPORATION; FLOW CYTOMETRY; GENE EXPRESSION; GENE THERAPY; GREEN FLUORESCENT PROTEINS; HUMANS; LUMINESCENT PROTEINS; MALE; OSTEOBLASTS; RATS; RATS, INBRED LEW; TRANSFECTION; TRANSGENES;

ANIMALIA; MUS;

EID: 0742289459     PISSN: 09697128     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.gt.3302163     Document Type: Article

References (16)

1. Piersma AH, Ploemacher RE, Brockbank KG. Radiation damage to femoral hemopoietic stroma measured by implant regeneration and quantitation of fibroblastic progenitors. Exp Hematol 1983; 11: 884-890.
2. Owen M, Freidenstein AJ. Stromal stem cells: marrow-derived osteogenic precursors. Cell and Molecular Biology of Vertebrate Hard Tissues, CIBA Foundation Symposium, Vol 136 1988, pp 42-60.
3. Caplan AI. Mesenchymal stem cells. J Orthop Res 1991; 9: 641-650.
4. Freidenstein AJ, Gorskaja U, Kalugina NN. Fibroblast precursors in normal and irradiated mouse hematopoietic organs. Exp Hematol 1976; 4: 267-274.
5. Wakitani S, Saito T, Caplan AI. Myogenic cells derived from rat bone marrow mesenchymal stem cells exposed to 5-azacytidine. Muscle Nerve 1995; 18: 1417-1426.
6. Bednarik DP. DNA methylation and retrovirus expression. EXS 1993; 64: 300-329.
7. Svoboda J et al. Retroviruses in foreign species and the problem of provirus silencing. Gene 2000; 261: 181-188.
8. Tsukiyama T, Niwa O, Yokoro K. Mechanism of suppression of the long terminal repeat of Moloney leukemia virus in mouse embryonal carcinoma cells. Mol Cell Biol 1989; 9: 4670-4676.
9. Keating A, Horsfall W, Hawley RG, Toneguzzo F. Effect of different promoters on expression of genes introduced into hematopoietic and marrow stromal cells by electroporation. Exp Hematol 1990; 18: 99-102.
10. Matthews KE, Dev SB, Toneguzzo F, Keating A. Electroporation for gene therapy. Methods Mol Biol 1995; 48: 273-280.
11. Colter DC, Class R, DiGirolamo CM, Prockop DJ. Rapid expansion of recycling stem cells in cultures of plastic-adherent cells from human bone marrow. Proc Natl Acad Sci USA 2000; 97: 3213-3218.
12. Digirolamo CM et al. Propagation and senescence of human marrow stromal cells in culture: a simple colony-forming assay identifies samples with the greatest potential to propagate and differentiate. Br J Haematol 1999; 107: 275-281.
13. Javazon EH, Colter DC, Schwarz EJ, Prockop DJ. Rat marrow stromal cells are more sensitive to plating density and expand more rapidly from single-cell-derived colonies than human marrow stromal cells. Stem Cells 2001; 19: 219-225.
14. Erbacher P, Roche AC, Monsigny M, Midoux P. Putative role for chloroquine in gene transfer into human hepatoma cell line by DNA/lactosylated polylysine complexes. Exp Cell Res 1996; 225: 186-194.
15. Hasan MT, Subbaroyan R, Chang TY. High efficiency stable gene transfection ushing chloroquine-treated Chinese hamster ovary cells. Somat Cell Mol Genet 1991; 17: 513-517.
16. Luthman H, Magnusson G. High efficiency polyoma DNA transfection of chloroquine treated cells. Nucleic Acids Res 1983; 11: 1295-1308.