Promoted adipogenesis of rat mesenchymal stem cells by transfection of small interfering rna complexed with a cationized dextran

Tissue Engineering - Part A

Volumn 16, Issue 1, 2010, Pages 21-31

  Nagane, Kentaro ^a   Jo, Jun Ichiro ^a   Tabata, Yasuhiko ^a  

^a KYOTO UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BIOACTIVITY; CELL CULTURE; DEXTRAN; FLOWCHARTING; NUCLEIC ACIDS; PHOSPHORUS; RNA; STEM CELLS; TRANSCRIPTION;

ADIPOGENESIS; ADIPOGENIC; BINDING MOTIF; BIOCHEMICAL EXPERIMENTS; BONE MARROW-DERIVED MESENCHYMAL STEM CELLS; CATIONIZED; CELL DIFFERENTIATION; COACTIVATORS; EXPRESSION LEVELS; GENE EXPRESSION LEVELS; GREEN FLUORESCENCE PROTEINS; HYDROXYL GROUPS; MESENCHYMAL STEM CELL; MOLAR PERCENTAGE; MOLAR RATIO; NITROGEN MOLECULE; NONVIRAL; OSTEOGENIC DIFFERENTIATION; PHYSICOCHEMICAL PROPERTY; SMALL INTERFERING RNA; SMALL INTERFERING RNA (SIRNA);

GLUCOSE;

RATTUS;

DEXTRAN; SIGNAL PEPTIDE; SMALL INTERFERING RNA; SPERMINE;

ADIPOGENESIS; ANIMAL; ARTICLE; BIOSYNTHESIS; CHEMISTRY; CYTOLOGY; DRUG EFFECT; GENE EXPRESSION REGULATION; GENETIC TRANSFECTION; GENETICS; MALE; MESENCHYMAL STEM CELL; METABOLISM; METHODOLOGY; PHYSIOLOGY; PROTEIN MOTIF; RAT; WISTAR RAT;

ADIPOGENESIS; AMINO ACID MOTIFS; ANIMALS; DEXTRANS; GENE EXPRESSION REGULATION; INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS; MALE; MESENCHYMAL STEM CELLS; RATS; RATS, WISTAR; RNA, SMALL INTERFERING; SPERMINE; TRANSFECTION;

EID: 77049122091     PISSN: 19373341     EISSN: 1937335X     Source Type: Journal    
DOI: 10.1089/ten.tea.2009.0170     Document Type: Article

References (82)

1. Ratko, T.A., Cummings, J.P., Blebea, J., and Matuszewski, K.A. Clinical gene therapy for nonmalignant disease. Am J Med 115, 560, 2003.
2. Mountain, A. Gene therapy: the first decade. Trends Bio-technol 18, 119, 2000.
3. Nemunaitis, J., Fong, T., Robbins, J.M., Edelman, G., Edwards, W., Paulson, R.S., Bruce, J., Ognoskie, N., Wynne, D., Pike, M., Kowal, K., Merritt, J., and Ando, D. Phase I trial of interferon-gamma (IFN-gamma) retroviral vector administered intratumorally to patients with metastatic melanoma. Cancer Gene Ther 6, 322, 1999.
4. Vecil, G.G., and Lang, F.F. Clinical trials of adenoviruses in brain tumors: a review of Ad-p53 and oncolytic adeno-viruses. J Neurooncol 65, 237, 2003.
5. Riddell, S.R., Elliott, M., Lewinsohn, D.A., Gilbert, M.J., Wilson, L., Manley, S.A., Lupton, S.D., Overell, R.W., Reynolds, T.C., Corey, L., and Greenberg, P.D. T-cell mediated rejection of gene-modified HIV-specific cytotoxic T lymphocytes in HIV-infected patients. Nat Med 2, 216, 1996.
6. Blaese, R.M., Culver, K.W., Miller, A.D., Carter, C.S., Fleisher, T., Clerici, M., Shearer, G., Chang, L., Chiang, Y., Tolstoshev, P., Greenblatt, J.J., Rosenberg, S.A., Klein, H., Berger, M., Mullen, C.A., Ramsey, W.J., Muul, L., Morgan, R.A., and Anderson, W.F. T lymphocyte-directed gene therapy for ADA-SCID: initial trial results after 4 years. Science 270, 475, 1995.
7. Elbashir, S.M., Harborth, J., Lendeckel, W., Yalcin, A., Weber, K., and Tuschl, T. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 411, 494, 2001.
8. Gilmore, I.R., Fox, S.P., Hollins, A.J., and Akhtar, S. Delivery strategies for siRNA-mediated gene silencing. Curr Drug Deliv 3, 147, 2006.
9. Li, C.X., Parker, A., Menocal, E., Xiang, S., Borodyansky, L., and Fruehauf, J.H. Delivery of RNA interference. Cell Cycle 5, 2103, 2006.
10. Tan, F.L., and Yin, J.Q. Application of RNAi to cancer research and therapy. Front Biosci 10, 1946, 2005.
11. Tuschl, T., and Borkhardt, A. Small interfering RNAs: a revolutionary tool for the analysis of gene function and gene therapy. Mol Interv 2, 158, 2002.
12. Audouy, S.A., de Leij, L.F., Hoekstra, D., and Molema, G. In vivo characteristics of cationic liposomes as delivery vectors for gene therapy. Pharm Res 19, 1599, 2002.
13. Voinea, M., and Simionescu, M. Designing of "intelligent" liposomes for efficient delivery of drugs. J Cell Mol Med 6, 465, 2002.
14. Pedroso de Lima, M.C., Neves, S., Filipe, A., Duzgunes, N., and Simoes, S. Cationic liposomes for gene delivery: from biophysics to biological applications. Curr Med Chem 10, 1221, 2003.
15. Nah, J.W., Yu, L., Han, S.O., Ahn, C.H., and Kim, S.W. Artery wall binding peptide-poly(ethylene glycol)-grafted-poly(L-lysine)-based gene delivery to artery wall cells. J Control Release 78, 273, 2002.
16. Lee, H., Jeong, J.H., and Park, T.G. PEG grafted polylysine with fusogenic peptide for gene delivery: high transfection efficiency with low cytotoxicity. J Control Release 79, 283, 2002.
17. Molas, M., Bartrons, R., and Perales, J.C. Single-stranded DNA condensed with poly-L-lysine results in nanometric particles that are significantly smaller, more stable in physiological ionic strength fluids and afford higher efficiency of gene delivery than their double-stranded counterparts. Bio-chim Biophys Acta 1572, 37, 2002.
18. Jeon, E., Kim, H.D., and Kim, J.S. Pluronic-grafted poly-(L)-lysine as a new synthetic gene carrier. J Biomed Mater Res 66, 854, 2003.
19. Remy-Kristensen, A., Clamme, J.P., Vuilleumier, C., Kuhry, J.G., and Mely, Y. Role of endocytosis in the transfection of L929 fibroblasts by polyethylenimine=DNA complexes. Biochim Biophys Acta 1514, 21, 2001.
20. Wightman, L., Kircheis, R., Rossler, V., Carotta, S., Ruzicka, R., Kursa, M., and Wagner, E. Different behavior of branched and linear polyethylenimine for gene delivery in vitro and in vivo. J Gene Med 3, 362, 2001.
21. Benns, J.M., Maheshwari, A., Furgeson, D.Y., Mahato, R.I., and Kim, S.W. Folate-PEG-folate-graft-polyethylenimine-based gene delivery. J Drug Target 9, 123, 2001.
22. Sagara, K., and Kim, S.W. A new synthesis of galactose-poly(ethylene glycol)-polyethylenimine for gene delivery to hepatocytes. J Control Release 79, 271, 2002.
23. Lee, H., Kim, T.H., and Park, T.G. A receptor-mediated gene delivery system using streptavidin and biotin-derivatized, pegylated epidermal growth factor. J Control Release 83, 109, 2002.
24. Kursa, M., Walker, G.F., Roessler, V., Ogris, M., Roedl, W., Kircheis, R., and Wagner, E. Novel shielded transferrin-polyethylene glycol- polyethylenimine=DNA complexes for systemic tumor-targeted gene transfer. Bioconjug Chem 14, 222, 2003.
25. Pittenger, M.F., Mackay, A.M., Beck, S.C., Jaiswal, R.K., Douglas, R., Mosca, J.D., Moorman, M.A., Simonetti, D.W., Craig, S., and Marshak, D.R. Multilineage potential of adult human mesenchymal stem cells. Science 284, 143, 1999.
26. Niemeyer, P., Krause, U., Kasten, P., Kreuz, P.C., Henle, P., Sudkam, N.P., and Mehlhorn, A. Mesenchymal stem cell-based HLA-independent cell therapy for tissue engineering of bone and cartilage. Curr Stem Cell Res Ther 1, 21, 2006.
27. Gimble, J.M., Katz, A.J., and Bunnell, B.A. Adipose-derived stem cells for regenerative medicine. Circ Res 100, 1249, 2007.
28. Tapp, H., Hanley, E.N., Jr., Patt, J.C., and Gruber, H.E. Adipose-derived stem cells: characterization and current application in orthopaedic tissue repair. Exp Biol Med 234, 1, 2009.
29. Jo, J., Nagaya, N., Miyahara, Y., Kataoka, M., Harada-Shiba, M., Kangawa, K., and Tabata, Y. Transplantation of genetically engineered mesenchymal stem cells improves cardiac function in rats with myocardial infarction: benefit of a novel nonviral vector, cationized dextran. Tissue Eng 13, 313, 2007.
30. Asahara, T., Kalka, C., and Isner, J.M. Stem cell therapy and gene transfer for regeneration. Gene Ther 7, 451, 2000.
31. Dumitriu, S., ed. Polysaccharides in Medical Applications. New York: Marcel Dekker Inc., 1996.
32. Mehvar, R. Recent trends in the use of polysaccharides for improved delivery of therapeutic agents: pharmacokinetic and pharmacodynamic perspectives. Curr Pharm Biotechnol 4, 283, 2003.
33. Mehvar, R., and Shepard, T.L. Molecular-weight-dependent pharmacokinetics of fluorescein-labeled dextrans in rats. J Pharm Sci 81, 908, 1992.
34. Mehvar, R., Robinson, M.A., and Reynolds, J.M. Molecular weight dependent tissue accumulation of dextrans: in vivo studies in rats. J Pharm Sci 83, 1495, 1994.
35. Harada, M., Murata, J., Sakamura, Y., Sakakibara, H., Okuno, S., and Suzuki, T. Carrier and dose effects on the pharmacokinetics of T-0128, a camptothecin analogue-carboxymethyl dextran conjugate, in non-tumor-and tumor-bearing rats. J Control Release 71, 71, 2001.
36. Masuda, K., Sakagami, M., Horie, K., Nogusa, H., Hamana, H., and Hirano, K. Evaluation of carboxymethylpullulan as a novel carrier for targeting immune tissues. Pharm Res 18, 217, 2001.
37. Guu, J.A., Hsiue, G.H., and Juang, T.M. Synthesis and biological properties of antitumor-active conjugates of ADR with dextran. J Biomater Sci Polym Ed 13, 1135, 2002.
38. Nogusa, H., Yamamoto, K., Yano, T., Kajiki, M., Hamana, H., and Okuno, S. Distribution characteristics of carbox-ymethylpullulan-peptide-doxorubicin conjugates in tumor-bearing rats: different sequence of peptide spacers and doxorubicin contents. Biol Pharm Bull 23, 621, 2000.
39. Nogusa, H., Yano, T., Kajiki, M., Gonsho, A., Hamana, H., and Okuno, S. Antitumor effects and toxicities of carbox-ymethylpullulan-peptide-doxorubicin conjugates. Biol Pharm Bull 20, 1061, 1997.
40. Nogusa, H., Yano, T., Okuno, S., Hamana, H., and Inoue, K. Synthesis of carboxymethylpullulan-peptide-doxorubicin conjugates and their properties. Chem Pharm Bull 43, 1931, 1995.
41. Ohya, Y., Oue, H., Nagatomi, K., and Ouchi, T. Design of macromolecular prodrug of cisplatin using dextran with branched galactose units as targeting moieties to hepatoma cells. Biomacromolecules 2, 927, 2001.
42. Yamaoka, T., Tabata, Y., and Ikada, Y. Body distribution profile of polysaccharide after intravenous administration. Drug Deliv 1, 75, 1993.
43. Kaneo, Y., Tanaka, T., Nakano, T., and Yamaguchi, Y. Evidence for receptor-mediated hepatic uptake of pullulan in rats. J Control Release 70, 365, 2001.
44. Dansereau, R.N., and Line, B.R. Preparation of dextran 70 injection labeled with technetium 99m for use as a cardiac blood-pool imaging agent. Am J Hosp Pharm 51, 2797, 1994.
45. Bhatnagar, A., Singh, A.K., Babbar, A., Soni, N.L., and Singh, T. Renal imaging with 99Tc(m)-dextran. Nucl Med Commun 18, 562, 1997.
46. Neubauer, S., Mena, I., Iglesis, R., Schwartz, R., Acevedo, J.C., Leon, A., and Gomez, L. Sentinel lymph node mapping in melanoma with technetium-99m dextran. Cancer Biother Radiopharm 16, 265, 2001.
47. Sivaratnam, D.A., Pitman, A.G., Giles, E., and Lichtenstein, M. The utility of Tc-99m dextran in the diagnosis and identification of melanoma metastases responsible for protein-losing enteropathy. Clin Nucl Med 27, 243, 2002.
48. Wang, S.C., Wikstrom, M.G., White, D.L., Klaveness, J., Holtz, E., Rongved, P., Moseley, M.E., and Brasch, R.C. Evaluation of Gd-DTPA-labeled dextran as an intravascular MR contrast agent: imaging characteristics in normal rat tissues. Radiology 175, 483, 1990.
49. Meyer, D., Schaefer, M., Chambon, C., and Beaute, S. Paramagnetic dextrans as magnetic resonance blood pool tracers. Invest Radiol 29 Suppl 2, S90, 1994.
50. Loubeyre, P., Canet, E., Zhao, S., Benderbous, S., Amiel, M., and Revel, D. Carboxymethyl-dextran-gadolinium-DTPA as a blood-pool contrast agent for magnetic resonance angi-ography. Experimental study in rabbits. Invest Radiol 31, 288, 1996.
51. Rebizak, R., Schaefer, M., and Dellacherie, E. Polymeric conjugates of Gd(3\+)-diethylenetriaminepentaacetic acid and dextran. 2. Influence of spacer arm length and conjugate molecular mass on the paramagnetic properties and some biological parameters. Bioconjug Chem 9, 94, 1998.
52. Rebizak, R., Schaefer, M., and Dellacherie, E. Polymeric conjugates of Gd(3\+)-diethylenetriaminepentaacetic acid and dextran. 1. Synthesis, characterization, and paramagnetic properties. Bioconjug Chem 8, 605, 1997.
53. Jo, J., and Tabata, Y. Non-viral gene transfection technologies for genetic engineering of stem cells. Eur J Pharm Bio-pharm 68, 90, 2008.
54. Hong, J.H., Hwang, E.S., McManus, M.T., Amsterdam, A., Tian, Y., Kalmukova, R., Mueller, E., Benjamin, T., Spiegel-man, B.M., Sharp, P.A., Hopkins, N., and Yaffe, M.B. TAZ, a transcriptional modulator of mesenchymal stem cell differentiation. Science 309, 1074, 2005.
55. Hong, J.H., and Yaffe, M.B. TAZ: a beta-catenin-like molecule that regulates mesenchymal stem cell differentiation. Cell Cycle 5, 176, 2006.
56. Kanai, F., Marignani, P.A., Sarbassova, D., Yagi, R., Hall, R.A., Donowitz, M., Hisaminato, A., Fujiwara, T., Ito, Y., Cantley, L.C., and Yaffe, M.B. TAZ: a novel transcriptional co-activator regulated by interactions with 14-3-3 and PDZ domain proteins. EMBO J 19, 6778, 2000.
57. Hermanson, G.T. Bioconjugate Techniques. San Diego, CA: Academic Press, 1996.
58. Hakamata, Y., Tahara, K., Uchida, H., Sakuma, Y., Naka-mura, M., Kume, A., Murakami, T., Takahashi, M., Taka-hashi, R., Hirabayashi, M., Ueda, M., Miyoshi, I., Kasai, N., and Kobayashi, E. Green fluorescent protein-transgenic rat: a tool for organ transplantation research. Biochem Biophys Res Commun 286, 779, 2001.
59. Manunta, M., Tan, P.H., Sagoo, P., Kashefi, K., and George, A.J. Gene delivery by dendrimers operates via a cholesterol dependent pathway. Nucleic Acids Res 32, 2730, 2004.
60. Lampariello, F. Evaluation of the number of positive cells from flow cytometric immunoassays by mathematical modeling of cellular autofluorescence. Cytometry 15, 294, 1994.
61. Hauner, H., Entenmann, G., Wabitsch, M., Gaillard, D., Ailhaud, G., Negrel, R., and Pfeiffer, E.F. Promoting effect of glucocorticoids on the differentiation of human adipocyte precursor cells cultured in a chemically defined medium. J Clin Invest 84, 1663, 1989.
62. Inoue, S., Hori, Y., Hirano, Y., Inamoto, T., and Tabata, Y. Effect of culture substrate and fibroblast growth factor addition on the proliferation and differentiation of human adipo-stromal cells. J Biomater Sci 16, 57, 2005.
63. Vaheri, A., and Pagano, J.S. Infectious poliovirus RNA: a sensitive method of assay. Virology 27, 434, 1965.
64. Janes, K.A., Calvo, P., and Alonso, M.J. Polysaccharide colloidal particles as delivery systems for macromolecules. Adv Drug Deliv Rev 47, 83, 2001.
65. Borchard, G. Chitosans for gene delivery. Adv Drug Deliv Rev 52, 145, 2001.
66. Ishii, T., Okahata, Y., and Sato, T. Mechanism of cell trans-fection with plasmid=chitosan complexes. Biochim Biophys Acta 1514, 51, 2001.
67. Huang, M., Fong, C.W., Khor, E., and Lim, L.Y. Transfection efficiency of chitosan vectors: effect of polymer molecular weight and degree of deacetylation. J Control Release 106, 391, 2005.
68. Guang Liu, W., and de Yao, K. Chitosan and its derivatives-a promising non-viral vector for gene transfection. J Control Release 83, 1, 2002.
69. Kim, Y.H., Gihm, S.H., Park, C.R., Lee, K.Y., Kim, T.W., Kwon, I.C., Chung, H., and Jeong, S.Y. Structural characteristics of size-controlled self-aggregates of deoxycholic acid-modified chitosan and their application as a DNA delivery carrier. Bioconjug Chem 12, 932, 2001.
70. Lee, K.Y., Kwon, I.C., Kim, Y.H., Jo, W.H., and Jeong, S.Y. Preparation of chitosan self-aggregates as a gene delivery system. J Control Release 51, 213, 1998.
71. Nagasaki, T., Hojo, M., Uno, A., Satoh, T., Koumoto, K., Mizu, M., Sakurai, K., and Shinkai, S. Long-term expression with a cationic polymer derived from a natural polysac-charide: schizophyllan. Bioconjug Chem 15, 249, 2004.
72. Matsumoto, T., Numata, M., Anada, T., Mizu, M., Koumoto, K., Sakurai, K., Nagasaki, T., and Shinkai, S. Chemically modified polysaccharide schizophyllan for antisense oligo-nucleotides delivery to enhance the cellular uptake efficiency. Biochim Biophys Acta 1670, 91, 2004.
73. Azzam, T., Eliyahu, H., Shapira, L., Linial, M., Barenholz, Y., and Domb, A.J. Polysaccharide-oligoamine based conjugates for gene delivery. J Med Chem 45, 1817, 2002.
74. Azzam, T., Raskin, A., Makovitzki, A., Brem, H., Vierling, P., Lineal, M., and Domb, A.J. Cationic polysaccharides for gene delivery. Macromolecules 35, 9947, 2002.
75. Azzam, T., Eliyahu, H., Makovitzki, A., Linial, M., and Domb, A.J. Hydrophobized dextran-spermine conjugate as potential vector for in vitro gene transfection. J Control Release 96, 309, 2004.
76. Jo, J., Ikai, T., Okazaki, A., Yamamoto, M., Hirano, Y., and Tabata, Y. Expression profile of plasmid DNA by spermine derivatives of pullulan with different extents of spermine introduced. J Control Release 118, 389, 2007.
77. Jo, J., Ikai, T., Okazaki, A., Nagane, K., Yamamoto, M., Hirano, Y., and Tabata, Y. Expression profile of plas-mid DNA obtained using spermine derivatives of pullulan with different molecular weights. J Biomater Sci 18, 883, 2007.
78. Son, K.K., Tkach, D., and Patel, D.H. Zeta potential of transfection complexes formed in serum-free medium can predict in vitro gene transfer efficiency of transfection reagent. Biochim Biophys Acta 1468, 11, 2000.
79. Nishikawa, M., and Huang, L. Nonviral vectors in the new millennium: delivery barriers in gene transfer. Hum Gene Ther 12, 861, 2001.
80. Zhou, X., and Huang, L. DNA transfection mediated by cationic liposomes containing lipopolylysine: characterization and mechanism of action. Biochim Biophys Acta 1189, 195, 1994.
81. Hosseinkhani, H., Aoyama, T., Yamamoto, S., Ogawa, O., and Tabata, Y. In vitro transfection of plasmid DNA by amine derivatives of gelatin accompanied with ultrasound irradiation. Pharm Res 19, 1471, 2002.
82. Kuwabara, T., Hsieh, J., Nakashima, K., Taira, K., and Gage, F.H. A small modulatory dsRNA specifies the fate of adult neural stem cells. Cell 116, 779, 2004.