High-efficiency DNA injection into a single human mesenchymal stem cell using a nanoneedle and atomic force microscopy

Nanomedicine: Nanotechnology, Biology, and Medicine

Volumn 4, Issue 3, 2008, Pages 215-225

  Han, Sung Woong ^a,b   Nakamura, Chikashi ^a,c   Kotobuki, Noriko ^a   Obataya, Ikuo ^a   Ohgushi, Hajime ^a   Nagamune, Teruyuki ^b   Miyake, Jun ^a,b,c  

^a NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

^b UNIVERSITY OF TOKYO   (Japan)

^c TOKYO UNIVERSITY OF AGRICULTURE AND TECHNOLOGY   (Japan)

Author keywords

Atomic force microscopy (AFM); Confocal microscopy; Gene expression; Gene transfer; Mesenchymal stem cells; Nanoindentation

Indexed keywords

AMINO ACIDS; ANIMAL CELL CULTURE; ATOMIC FORCE MICROSCOPY; ATOMIC PHYSICS; ATOMS; BIOTECHNOLOGY; CELL CULTURE; CELLS; COMMINUTION; CYTOLOGY; DNA; FLOWCHARTING; FOOD PRESERVATION; GENE TRANSFER; GENES; MICROCIRCULATION; MICROSCOPIC EXAMINATION; NANONEEDLES; NANOSTRUCTURES; NUCLEIC ACIDS; ORGANIC ACIDS; SCANNING PROBE MICROSCOPY; TISSUE ENGINEERING;

ATOMIC FORCE MICROSCOPY (AFM); CELL DAMAGES; CELL NUCLEUS; CELLULAR DAMAGE; CONFOCAL MICROSCOPY; GENE DELIVERY; GENE EXPRESSION; GREEN FLUORESCENT PROTEIN; HIGH-EFFICIENCY; HIGHLY EFFICIENT; HUMAN EMBRYONIC KIDNEY CELLS; HUMAN MESENCHYMAL STEM CELLS; LIPOFECTION; MESENCHYMAL STEM CELLS; MICRO INJECTION; NANOINDENTATION; NON-VIRAL GENE DELIVERY; POLY-L-LYSINE; SINGLE CELLS;

IMAGING TECHNIQUES;

DNA; GREEN FLUORESCENT PROTEIN; LIPOFECTIN; POLYLYSINE;

ADULT; AGED; ARTICLE; ATOMIC FORCE MICROSCOPY; CELL DAMAGE; CELL NUCLEUS; CELL STRAIN HEK293; EMBRYO; GENE; GENE TARGETING; HUMAN; HUMAN CELL; MESENCHYMAL STEM CELL; MICROINJECTION; NANODEVICE; NANONEEDLE; NONVIRAL GENE DELIVERY SYSTEM; PLASMID;

CELL LINE; CELL NUCLEUS; DNA; GREEN FLUORESCENT PROTEINS; HUMANS; MESENCHYMAL STEM CELLS; MICROINJECTIONS; MICROSCOPY, ATOMIC FORCE; NANOTECHNOLOGY; PLASMIDS; TRANSFECTION;

EID: 49649127358     PISSN: 15499634     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.nano.2008.03.005     Document Type: Article

References (29)

1. Hahn W., Ho S.H., Jeong J.G., Hahn E.Y., Kim S., Yu S.S., et al. Viral vector-mediated transduction of a modified thrombospondin-2 cDNA inhibits tumor growth and angiogenesis. Gene Ther 11 (2004) 739-745
2. Selkirk S.M., Green S.J., Plunkett R.J., Baron T.A., Lis A., and Spence P.O. Syngeneic central nervous system transplantation of genetically transduced mature, adult astrocytes. Gene Ther 9 (2002) 432-443
3. Kang Y., Stein C.S., Heth J.A., Sinn P.L., Penisten A.K., Staber P.D., et al. In vivo gene transfer using a nonprimate lentiviral vector pseudotyped with Ross river virus glycoproteins. J Virol 76 (2002) 9378-9388
4. Modlich U., Bohne J., Schmidt M., von Kalle C., Knöss S., Schambach A., et al. Cell-culture assays reveal the importance of retroviral vector design for insertional genotoxicity. Blood 108 (2006) 2545-2553
5. Mathis J.M., Williams B.J., Sibley D.A., Carroll J.L., Li J., Odaka Y., et al. Cancer-specific targeting of an adenovirus-delivered herpes simplex virus thymidine kinase suicide gene using translational control. J Gene Med 8 (2006) 1105-1120
6. Bruder J.T., and Kovesdi I. Adenovirus infection stimulates the Raf/MAPK signaling pathway and induces interleukin-8 expression. J Virol 71 (1997) 398-404
7. Shifrin A.L., Chirmule N., Gao G.P., Wilson J.M., and Raper S.E. Innate immune responses to adenoviral vector-mediated acute pancreatitis. Pancreas 30 (2005) 122-129
8. Wang S., Baum B.J., Yamano S., Mankani M.H., Sun D., Jonsson M., et al. Adenoviral-mediated gene transfer to mouse salivary glands. J Dent Res 79 (2000) 701-708
9. Bieber T., Meissner W., Kostin S., Niemann A., and Elsasser H.P. Intracellular route and transcriptional competence of polyethylenimine-DNA complexes. J Control Release 82 (2002) 441-454
10. Hoelters J., Ciccarella M., Drechsel M., Geissler C., Gulkan H., Bocker W., et al. Nonviral genetic modification mediates effective transgene expression and functional RNA interference in human mesenchymal stem cells. J Gene Med 7 (2005) 718-728
11. Pollard H., Remy J.S., Loussouarn G., Demolombe S., Behr J.P., and Escande D. Polyethylenimine but not cationic lipids promotes transgene delivery to the nucleus in mammalian cells. J Biol Chem 273 (1998) 7507-7511
12. Vijayanathan V., Thomas T., and Thomas T.J. DNA nanoparticles and development of DNA delivery vehicles for gene therapy. Biochemistry 41 (2002) 14085-14094
13. Jeschke M.G., Barrow R.E., Hawkins H.K., Yang K., Hayes R.L., Lichtenbelt B.J., et al. IGF-I gene transfer in thermally injured rats. Gene Ther 6 (1999) 1015-1020
14. Babiuk S., van Drunen Littel-van den Hurk S., and Babiuk L.A. Delivery of DNA vaccines using electroporation. Methods Mol Med 127 (2006) 73-82
15. Knoblauch M., Hibberd J.M., Gray J.C., and van Bell A.J. A galinstan expansion femtosyringe for microinjection of eukaryotic organelles and prokaryotes. Nat Biotechnol 17 (1999) 906-909
16. Tsulaia T.V., Prokopishyn N.L., Yao A., Carsrud N.D.V., Carou M.C., Brown D.B., et al. Glass needle-mediated microinjection of macromolecules and transgenes into primary human mesenchymal stem cells. J Biomed Sci 10 (2003) 328-336
17. Bruckbauer A., Ying L., Rothery A.M., Zhou D., Shevchuk A.I., Abell C., et al. Writing with DNA and protein using a nanopipet for controlled delivery. J Am Chem Soc 124 (2002) 8810-8811
18. Agarwal G., Naik R.R., and Stone M.O. Immobilization of histidine-tagged proteins on nickel by electrochemical dip pen nanolithography. J Am Chem Soc 125 (2003) 7408-7412
19. Demers L.M., Ginger D.S., Park S.J., Li Z., Chung S.W., and Mirkin C.A. Direct patterning of modified oligonucleotides on metals and insulators by dip-pen nanolithography. Science 296 (2002) 1836-1838
20. Obataya I., Nakamura C., Han S., Nakamura N., and Miyake J. Nanoscale operation of a living cell using an atomic force microscope with a nanoneedle. Nano Lett 5 (2005) 27-30
21. Han S., Nakamura C., Obataya I., Nakamura N., and Miyake J. A molecular delivery system by using AFM and nanoneedle. Biosens Bioelectron 20 (2005) 2120-2125
22. Obataya I., Nakamura C., Han S., Nakamura N., and Miyake J. Mechanical sensing of the penetration of various nanoneedles into a living cell using atomic force microscopy. Biosens Bioelectron 20 (2005) 1652-1655
23. Han S., Nakamura C., Obataya I., Nakamura N., and Miyake J. Gene expression using an ultra thin needle enabling accurate displacement and low invasiveness. Biochem Biophys Res Commun 332 (2005) 633-639
24. Kotobuki N., Hirose M., Takakura Y., and Ohgushi H. Cultured autologous human cells for hard tissue regeneration: preparation and characterization of mesenchymal stem cells from bone marrow. Artif Organs 28 (2004) 33-39
25. Kotobuki N., Hirose M., Machida H., Katou Y., Muraki K., Takakura Y., et al. Viability and osteogenic potential of cryopreserved human bone marrow-derived mesenchymal cells. Tissue Eng 11 (2005) 663-673
26. Boron W.F. Regulation of intracellular pH. Adv Physiol Educ 28 (2004) 160-179
27. Cai D., Mataraza J.M., Qin Z.H., Huang Z., Huang J., Chiles T.C., et al. Highly efficient molecular delivery into mammalian cells using carbon nanotube spearing. Nat Methods 2 (2005) 449-454
28. Cuerrier C.M., Lebel R., and Grandbois M. Single cell transfection using plasmid decorated AFM probes. Biochem Biophys Res Commun 355 (2007) 632-636
29. Ryoji M., and Worcel A. Chromatin assembly in Xenopus oocytes: in vivo studies. Cell 37 (1984) 21-32