Bromelain: An overview of industrial application and purification strategies

Applied Microbiology and Biotechnology

Volumn 98, Issue 17, 2014, Pages 7283-7297

  Arshad, Zatul Iffah Mohd ^a,b   Amid, Azura ^a   Yusof, Faridah ^a   Jaswir, Irwandi ^a   Ahmad, Kausar ^a   Loke, Show Pau ^c  

^a INTERNATIONAL ISLAMIC UNIVERSITY MALAYSIA   (Malaysia)

^b UNIVERSITY MALAYSIA PAHANG   (Malaysia)

^c UNIVERSITY OF NOTTINGHAM MALAYSIA CAMPUS   (Malaysia)

Author keywords

Bromelain; Cysteine protease; Purification; Recombinant bromelain

Indexed keywords

AMINO ACIDS; PURIFICATION; TEXTILE INDUSTRY;

CYSTEINE PROTEASE; DOWNSTREAM-PROCESSING; LARGE SCALE PRODUCTIONS; NOVEL APPLICATIONS; PINEAPPLE FRUIT; TEMPERATURE CONDITIONS;

BROMELAIN;

BROMELAIN; PROTEIN HYDROLYSATE; TOOTH BLEACHING AGENT;

DNA; FRUIT; INDUSTRIAL DEVELOPMENT; PLANT EXTRACT; PURIFICATION; TECHNOLOGICAL DEVELOPMENT;

CHROMATOGRAPHY; COSMETIC INDUSTRY; HYDROPHOBICITY; LARGE SCALE PRODUCTION; PH; PINEAPPLE; PINEAPPLE JUICE; PURIFICATION; RECOMBINANT DNA TECHNOLOGY; REVERSE MICELLE; SHORT SURVEY; TEMPERATURE; TEXTILE INDUSTRY; ULTRAFILTRATION; ANANAS; BIOTECHNOLOGY; ENZYMOLOGY; ISOLATION AND PURIFICATION; METABOLISM; PROCEDURES;

ANANAS COMOSUS;

ANANAS; BIOTECHNOLOGY; BROMELAINS;

EID: 84906948417     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-014-5889-y     Document Type: Short Survey

References (121)

1. Abdelgadir IEO, Cochran RC, Titgemeyer EC, Vanzant ES (2013) In vitro determination of ruminal protein degradability of alfafa and prairie hay via a commercial protease in the presence or absence of cellulase or driselase. J Anim Sci 75:2215-2222
2. Amid A, Ismail NA, Yusof F, Salleh HM(2011) Expression, purification, and characterization of a recombinant stem bromelain from Ananas comosus. Process Biochem 46(12):2232-2239
3. Azevedo AM, Rosa PAJ, Ferreira IF, Aires-Barros MR (2009) Chromatography-free recovery of biopharmaceuticals through aqueous two-phase processing. Trends Biotechnol 27(4):240-247. doi:10.1016/j.tibtech.2009.01.004
4. Babu BR, Rastogi NK, Raghavarao KSMS (2008) Liquid-liquid extraction of bromelain and polyphenol oxidase using aqueous two-phase system. Chem Eng Process Process Intensif 47(1):83-89. doi:10.1016/j.cep.2007.08.006 (Pubitemid 47625691)
5. Barrett AJ, Kembhavi AA, Brown MA, Kirschke H, Knight CG, Tamai M, Hanada K (1982) L-trans-Epoxysuccinyl-leucylamido(4-guanidino)butane (E-64) and its analogues as inhibitors of cysteine proteinases including cathepsins B, H and L. Biochem J 201(1):189-198 (Pubitemid 12038477)
6. Benucci I, Liburdi K, Garzillo AMV, Esti M (2011) Bromelain from pineapple stem in alcoholic-acidic buffers for wine application. Food Chem 124(4):1349-1353. doi:10.1016/j.foodchem.2010.07.087
7. Bhambure R, Sharma R, Gupta D, Rathore AS (2013) A novel aqueous two phase assisted platform for efficient removal of process related impurities associated with E. coli based biotherapeutic protein products. J Chromatogr A. doi:10.1016/j.chroma.2013.07.085
8. Bhattacharya R, Bhattacharyya D (2009) Preservation of natural stability of fruit "bromelain" from Ananas comosus (pineapple). J Food Biochem 33(1):1-19
9. Bruno M, Trejo S, Avilés X, Caffini N, López L (2006) Isolation and characterization of hieronymain II, another peptidase isolated from fruits of Bromelia hieronymi Mez (Bromeliaceae). Protein J 25(3):224-231 (Pubitemid 44174987)
10. Chaisakdanugull C, Chockchai T, Wrolstad RE (2007) Pineapple juice and its fractions in enzymatic browning inhibition of banana [ Musa (AAA group) Gros Michel]. J Agric Food Chem 55:4252-4257 (Pubitemid 46867442)
11. Chaiwut P, Nitsawang S, Shank L, Kanasawud P (2007) A comparative study on properties and proteolytic components of papaya peel and latex proteases. Chiang Mai J Sci 34(1):109-118
12. Chakravarthy PK, Acharya S (2012) Efficacy of extrinsic stain removal by novel dentifrice containing papain and bromelain extracts. J Young Pharm 4(4):245-249. doi:10.4103/0975-1483.104368
13. Chalamaiah M, Dinesh Kumar B, Hemalatha R, Jyothirmayi T (2012) Fish protein hydrolysates: proximate composition, amino acid composition, antioxidant activities and applications: a review. Food Chem 135(4):3020-3038. doi:10.1016/j.foodchem.2012.06.100
14. Chaurasiya RS, Umesh Hebbar H (2013) Extraction of bromelain from pineapple core and purification by RME and precipitation methods. Sep Purif Technol 111(0):90-97. doi:10.1016/j.seppur.2013.03.029
15. Chen D-H, Huang S-H (2004) Fast separation of bromelain by polyacrylic acid-bound iron oxide magnetic nanoparticles. Process Biochem 39(12):2207-2211. doi:10.1016/j.procbio.2003.11.014 (Pubitemid 39123618)
16. Cheung IWY, Li-Chan ECY (2014) Application of taste sensing system for characterisation of enzymatic hydrolysates from shrimp processing by-products. Food Chem 145(0):1076-1085. doi:10.1016/j.foodchem.2013.09.004
17. Choe Y, Leonetti F, Greenbaum DC, Lecaille F, Bogyo M, Brömme D, Ellman JA, Craik CS (2006) Substrate profiling of cysteine proteases using a combinatorial peptide library identifies functionally unique specificities. J Biol Chem 281(18):12824-12832. doi:10.1074/jbc.M513331200 (Pubitemid 43855375)
18. Chuapoehuk P, Raksakulthai N (1992) Use of papain and bromelain in the production of oyster sauce. ASEAN Food J 7(4):196-199
19. Coelho DF, Silveira E, Pessoa Junior A, Tambourgi EB (2013) Bromelain purification through unconventional aqueous two-phase system (PEG/ammonium sulphate). Bioprocess Biosyst Eng 36(2):185-192. doi:10.1007/s00449-012-0774-5
20. Corzo CA, Waliszewski KN, Welti-Chanes J (2012) Pineapple fruit bromelain affinity to different protein substrates. Food Chem 133(3):631-635. doi:10.1016/j.foodchem.2011.05.119
21. Devakate RV, Patil VV, Waje SS, Thorat BN (2009) Purification and drying of bromelain. Sep Purif Technol 64(3):259-264
22. Devi RY (2012) Biotechnological application of proteolytic enzymes in post cocoon technology. Int J Sci Nat 3(2):237-240
23. Doko MB, Bassani V, Casadebaig J, Cavailles L, Jacob M (1991) Preparation of proteolytic enzyme extracts from Ananas comosus L., Merr. fruit juice using semipermeable membrane, ammonium sulfate extraction, centrifugation and freeze-drying processes. Int J Pharm 76(3):199-206. doi:10.1016/0378-5173(91) 90272-P
24. Elavarasan K, Naveen Kumar V, Shamasundar BA (2013) Antioxidant and functional properties of fish protein hydrolysates from fresh water carp (Catla catla) as influenced by the nature of enzyme. J Food Process Preserv:n/a-n/a. doi:10.1111/jfpp.12081
25. Espitia-Saloma E, Vázquez-Villegas P, Aguilar O, Rito-Palomares M (2013) Continuous aqueous two-phase systems devices for the recovery of biological products. Food Bioprod Process. doi:10.1016/j.fbp.2013.05.006
26. Feijoo-Siota L, Villa T (2011) Native and biotechnologically engineered plant proteases with industrial applications. Food Bioprocess Technol 4(6):1066-1088
27. Ferreira JF, Bresolin IRP, Silveira E, Tambourgi EB (2011) Purification of bromelain from Ananas comosus by PEG/phosphate ATPS. Chem Eng Trans 24:931-936
28. Fileti AMF, Fischer GA, Santana JCC, Tambourgi EB (2009) Batch and continuous extraction of bromelain enzyme by reversed micelles. Braz Arch Biol Techn 52:1225-1234
29. Gautam SS, Mishra SK, Dash V, Goyal AK, Rath G (2010) Comparative study of extraction, purification and estimation of bromelain from stem and fruit of pineapple plant. Thai J Pharm Sci 34:67-76
30. Gerelt B, Ikeuchi Y, Suzuki A (2000) Meat tenderization by proteolytic enzymes after osmotic dehydration. Meat Sci 56(3):311-318. doi:10.1016/S0309- 1740(00)00060-7
31. Ghanbari R, Ebrahimpour A, Hamid AA, Ismail A, Saari N (2012) Actinopyga lecanora hydrolysates as natural antibacterial agents. Int J Mol Sci 13:16796-16811. doi:10.3390/ijms131216796
32. Gimeno C, Seguí L, Fito P (2010) Valorisation of industrial pineapple residues: bromelain separation followed by bioethanol fermentation. In: Int Conf Food Innov Food Innova 2010, p 1-4
33. Givens DI, Owen E, Axford RFE, Omed HM(2002) Forage evaluation in ruminant nutrition. CABI Publishing
34. Gräslund S, Nordlund P, Weigelt J, Hallberg BM, Bray J, Gileadi O, Knapp S, Oppermann U, Arrowsmith C, Hui R, Ming J, dhe-Paganon S, Park H-w, Savchenko A, Yee A, Edwards A, Vincentelli R, Cambillau C, Kim R, Kim S-H, Rao Z, Shi Y, Terwilliger TC, Kim C-Y, Hung L-W, Waldo GS, Peleg Y, Albeck S, Unger T, Dym O, Prilusky J, Sussman JL, Stevens RC, Lesley SA, Wilson IA, Joachimiak A, Collart F, Dementieva I, Donnelly MI, Eschenfeldt WH, Kim Y, Stols L, Wu R, Zhou M, Burley SK, Emtage JS, Sauder JM, Thompson D, Bain K, Luz J, Gheyi T, Zhang F, Atwell S, Almo SC, Bonanno JB, Fiser A, Swaminathan S, William F, Studier, Chance MR, Sali A, Acton TB, Xiao R, Zhao L, Ma LC, Hunt JF, Tong L, Cunningham K, Inouye M, Anderson S, Janjua H, Shastry R, Ho CK, Wang D, Wang H, Jiang M, Montelione GT, Stuart DI, Owens RJ, Daenke S, Schütz A, Heinemann U, Yokoyama S, Büssow K, Gunsalus KC (2008) Protein production and purification. Nat Meth 5(2):135-146
35. Gupta P, Saleemuddin M (2006) Bioaffinity based oriented immobilization of stem bromelain. Biotechnol Lett 28(12):917-922 (Pubitemid 43926919)
36. Hage DS, Anguizola JA, Bi C, Li R, Matsuda R, Papastavros E, Pfaunmiller E, Vargas J, Zheng X (2012) Pharmaceutical and biomedical applications of affinity chromatography: recent trends and developments. J Pharm Biomed Anal:1-13
37. Hale LP, Greer PK, Trinh CT, James CL (2005) Proteinase activity and stability of natural bromelain preparations. Int Immunopharmacol 5(4):783-793 (Pubitemid 40248332)
38. Hannig C, Hannig M, Attin T (2005) Enzymes in the acquired enamel pellicle. Eur J Oral Sci 113(1):2-13. doi:10.1111/j.1600-0722.2004.00180.x (Pubitemid 40313833)
39. Hannig C, Spitzmüller B, Lux HC, Altenburger M, Al-Ahmad A, Hannig M (2010) Efficacy of enzymatic toothpastes for immobilisation of protective enzymes in the in situ pellicle. Arch Oral Biol 55(7):463-469. doi:10.1016/j.archoralbio.2010.03.020
40. Harrach T, Eckert K, Schulze-Forster K, Nuck R, Grunow D, Maurer HR (1995) Isolation and partial characterization of basic proteinases from stem bromelain. J Protein Chem 14(1):41-52. doi:10.1007/bf01902843
41. Harrach T, Eckert K, Maurer HR, Machleidt I, Machleidt W, Nuck R (1998) Isolation and characterization of two forms of an acidic bromelain stem proteinase. J Protein Chem 17(4):351-361. doi:10.1023/a:1022507316434 (Pubitemid 28242305)
42. Hebbar U, Sumana B, Hemavathi AB, Raghavarao KSMS (2012) Separation and purification of bromelain by reverse micellar extraction coupled ultrafiltration and comparative studies with other methods. Food Bioprocess Tech 5(3):1010-1018. doi:10.1007/s11947-010-0395-4
43. Heinicke R, Gortner W (1957) Stem bromelain - a new protease preparation from pineapple plants. Econ Bot 11(3):225-234
44. Hevia P, Olcott HS (1977) Flavor of enzyme-solubilized fish protein concentrate fractions. J Agric Food Chem 25(4):772-775. doi:10.1021/jf60212a044
45. Iversen SL, Jørgensen MH (1995) Azocasein assay for alkaline protease in complex fermentation broth. Biotechnol Tech 9(8):573-576. doi:10.1007/bf00152446
46. Janson J-C (2011) Protein purification: principles, high resolution methods, and applications, 3rd edition. John Wiley & Sons
47. Joiner A (2010) Whitening toothpastes: a review of the literature. J Dent 38:e17-e24
48. Kalyana P, Shashidhar A, Meghashyam B, SreeVidya KR, Sweta S (2011) Stain removal efficacy of a novel dentifrice containing papain and bromelain extracts - an in vitro study. Int J Dent Hyg 9(3):229-233. doi:10.1111/j.1601-5037.2010. 00473.x
49. Ketnawa S, Rawdkuen S (2011) Application of bromelain extract for muscle foods tenderization. Food Nutr Sci 2:393-401. doi:10.4236/fns.2011.25055
50. Ketnawa S, Rawdkuen S, Chaiwut P (2010) Two phase partitioning and collagen hydrolysis of bromelain from pineapple peel Nang Lae cultivar. Biochem Eng J 52(2-3):205-211. doi:10.1016/j.bej.2010.08.012
51. Ketnawa S, Chaiwut P, Rawdkuen S (2012) Pineapple wastes: a potential source for bromelain extraction. Food Bioprod Process 90(3):385-391
52. Khan R, Rasheedi S, Haq S (2003) Effect of pH, temperature and alcohols on the stability of glycosylated and deglycosylated stem bromelain. J Biosci 28(6):709-714. doi:10.1007/bf02708431 (Pubitemid 37522777)
53. Ko B-S, Hwang Y-I, Lee S-C (1996) Simple purification of bromelain from pineapple. J Food Sci Nutr 1(1):106-110
54. Koh J, Kang S-M, Kim S-J, Cha M-K, Kwon Y-J (2006) Effect of pineapple protease on the characteristics of protein fibers. Fibers Polym 7(2):180-185. doi:10.1007/bf02908264 (Pubitemid 44126351)
55. Kong X, Zhou H, Qian H (2007) Enzymatic hydrolysis of wheat gluten by proteases and properties of the resulting hydrolysates. Food Chem 102(3):759-763. doi:10.1016/j.foodchem.2006.06.062 (Pubitemid 46108278)
56. Koohmaraie M, Geesink GH (2006) Contribution of postmortem muscle biochemistry to the delivery of consistent meat quality with particular focus on the calpain system. Meat Sci 74(1):34-43. doi:10.1016/j.meatsci.2006.04.025 (Pubitemid 43975225)
57. Kumar V, Sharma VK, Kalonia DS (2009) Effect of polyols on polyethylene glycol (PEG)-induced precipitation of proteins: impact on solubility, stability and conformation. Int J Pharm 366(1-2):38-43. doi:10.1016/j.ijpharm.2008.08.037
58. Kumar S, Hemavathi AB, Hebbar HU (2011) Affinity based reverse micellar extraction and purification of bromelain from pineapple (Ananas comosus L. Merryl) waste. Process Biochem 46(5):1216-1220. doi:10.1016/j.procbio.2011.02. 008
59. Lamsal BP, Jung S, Johnson LA (2007) Rheological properties of soy protein hydrolysates obtained from limited enzymatic hydrolysis. LWT - Food SciTechnol 40(7):1215-1223. doi:10.1016/j.lwt.2006.08.021
60. Lan JC-W, Yeh C-Y, Wang C-C, Yang Y-H, Wu H-S (2013) Partition separation and characterization of the polyhydroxyalkanoates synthase produced fromrecombinant Escherichia coli using an aqueous two-phase system. J Biosci Bioeng 116(4):499-505. doi:10.1016/j.jbiosc.2013.04.010
61. Laohakunjit N, Selamassakul O, Kerdchoechuen O (2014) Seafood-like flavour obtained from the enzymatic hydrolysis of the protein byproducts of seaweed (Gracilaria sp.). Food Chem. doi:10.1016/j.foodchem.2014.02.101
62. Lee KL, Albee KL, Bernasconi RJ, Edmunds T (1997) Complete amino acid sequence of ananain and a comparison with stem bromelain and other plant cysteine proteases. Biochem J 327:199-202 (Pubitemid 27455479)
63. Levy LL, Emer JJ (2012) Complications of minimally invasive cosmetic procedures: prevention andmanagement. J Cutan Aesthet Surg 5(2):121-132. doi:10.4103/0974-2077.99451
64. Lizuka K, Aishima T (1999) Tenderization of beef with pineapple juice monitored by fourier transform infrared spectroscopy and chemometric analysis. J Food Sci 64(6):973-977. doi:10.1111/j.1365-2621.1999.tb12262.x
65. Lopes FLG, Severo Júnior JB, Souza RR, Ehrhardt DD, Santana JCC, Tambourgi EB (2009) Concentration by membrane separation processes of a medicinal product obtained from pineapple pulp. Braz Arch Biol Technol 52:457-464
66. Lozano-De-Gonzalez PG, Barrett DM, Wrolstad RE, Durst RW (1993) Enzymatic browning inhibited in fresh and dried apple rings by pineapple juice. J Food Sci 58(2):399-404
67. Melendo JA, Beltrán JA, Roncalés P (1997) Tenderization of squid (Loligo vulgaris and Illex coindetii) with bromelain and a bovine spleen lysosomal-enriched extract. Food Res Int 30(5):335-341. doi:10.1016/s0963- 9969(97)00057-4 (Pubitemid 28126521)
68. Murachi T (1964)Amino acid composition of stem bromelain*. Biochem 3(7):932-934. doi:10.1021/bi00895a014
69. Murachi T, Neurath H (1960) Fractionation and specificity studies on stem bromelain. J Biol Chem 235(1):99-107
70. Murachi T, Yasui M, Yasuda Y (1964) Purification and physical characterization of stem bromelain*. Biochem 3(1):48-55
71. Murachi T, Suzuki A, Takahashi N (1967) Evidence for glycoprotein nature of stem bromelain. Isolation of a glycopeptide*. Biochem 6(12):3730-3736. doi:10.1021/bi00864a015
72. Mutalib SRA, Samicho Z, Abdullah N, Zaman NK, Hajar N Effect of maturity of pineapple variety N36 on its waste physico-chemical properties. In: 2012 I.E. Colloq Hum Sci Eng Res 3-4 Dec. 2012 2012. p 432-436
73. Nadzirah KZ, Zainal S, Noriham A, Normah I, Roha AMS (2012) Physico-chemical properties of pineapple crown extract variety N36 and bromelain activity in different forms. APCBEE Procedia 4(0):130-134. doi:10.1016/j. apcbee.2012.11.022
74. Napper AD, Bennett SP, Borowski M, Holdridge MB, Leonard MJ, Rogers EE, Duan Y, Laursen RA, Reinhold B, Shames SL (1994) Purification and characterization of multiple forms of the pineapple-stem-derived cysteine proteinases ananain and comosain. Biochem J 301:727-735 (Pubitemid 24239305)
75. Nowak D (2011) Enzymes in tenderization of meat - the system of calpains and other systems - a review. Pol J Food and Nutr Sci 61(4):231-237. doi:10.2478/v10222-011-0025-5
76. Omidinia E, Shahbaz Mohamadi H, Dinarvand R, Taherkhani H-A (2010) Investigation of chromatography and polymer/salt aqueous two-phase processes for downstream processing development of recombinant phenylalanine dehydrogenase. Bioprocess and Biosyst Eng 33(3):317-329. doi:10.1007/s00449-009-0327-8
77. Ota S, Moore S, Stein WH (1964) Preparation and chemical properties of purified stem and fruit bromelains*. Biochem 3(2):180-185. doi:10.1021/bi00890a007
78. Ota S, Muta E, Katahira Y, Okamoto Y (1985) Reinvestigation of fractionation and some properties of the proteolytically active components of stem and fruit bromelains. J Biochem 98(1):219-228
79. Ozlen SN, Chatsworth C (1995) Cosmetic composition containing alpha hydroxyacids, salicyclic acid, and enzyme mixture of bromelain and papain. United States Patent 5:441,740
80. Ozoǧlu H, Bayindirli A (2002) Inhibition of enzymic browning in cloudy apple juice with selected antibrowning agents. Food Control 13(4-5):213-221. doi:10.1016/S0956-7135(02)00011-7
81. Pasupuleti VK, Braun S (2010) State of the art manufacturing of protein hydrolysates. In: Demain AL, Pasupuleti VK (eds) Protein hydrolysates in biotechnology. Springer, Netherlands, pp 11-32
82. Pietrasik Z, Shand PJ (2011) Effects of moisture enhancement, enzyme treatment, and blade tenderization on the processing characteristics and tenderness of beef semimembranosus steaks. Meat Sci 88(1):8-13. doi:10.1016/j.meatsci.2010.11.024
83. Pietrasik Z, Aalhus JL, Gibson LL, Shand PJ (2010) Influence of blade tenderization, moisture enhancement and pancreatin enzyme treatment on the processing characteristics and tenderness of beef semitendinosus muscle. Meat Sci 84(3):512-517
84. Polaina J, MacCabe AP (2007) Industrial enzymes: structure, function and applications. Springer, Netherland
85. Rabelo APB, Tambourgi EB, Pessoa A Jr (2004) Bromelain partitioning in two-phase aqueous systems containing PEO-PPO-PEO block copolymers. J Chromatogr, B 807(1):61-68. doi:10.1016/j.jchromb.2004.03.029 (Pubitemid 38721227)
86. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases. Microbiology and Molecular Biology Reviews 62(3):597-635 (Pubitemid 28415546)
87. Ren J, Zhao M, Shi J, Wang J, Jiang Y, Cui C, Kakuda Y, Xue SJ (2008) Purification and identification of antioxidant peptides from grass carp muscle hydrolysates by consecutive chromatography and electrospray ionization-mass spectrometry. Food Chem 108(2):727-736. doi:10.1016/j.foodchem.2007.11.010
88. Ritonja A, Rowan AD, Buttle DJ, Rawlings ND, Turk V, Barrett AJ (1989) Stem bromelain: amino acid sequence and implications for weak binding of cystatin. FEBS Lett 247(2):419-424. doi:10.1016/0014-5793(89)81383-3 (Pubitemid 19129290)
89. Rowan AD, Buttlet DJ, Barrett AJ (1990) The cysteine proteinases of the pineapple plant. Biochem J 266:869-875 (Pubitemid 20103724)
90. Sai-Nan S, Yu-Ting Z, Hua-Li N, Li-Min Z, Branford-White CJ (2009) Isotherm, Kinetic and thermodynamic analysis of bromelain adsorption on reactive blue 4 immobilized composite membranes. In: 3rd Int Conf Bioinf Biomed Eng ICBBE 2009 11-13 June 2009 p 1-4
91. Salampessy J, Phillips M, Seneweera S, Kailasapathy K (2010) Release of antimicrobial peptides through bromelain hydrolysis of leatherjacket (Meuchenia sp.) insoluble proteins. Food Chem 120(2):556-560. doi:10.1016/j.foodchem.2009. 10.054
92. Sangjindavong M, Mookdasanit J, Wilaipun P, Chuapoehuk P, Akkanvanitch C (2009) Using pineapple to produce fish sauce from surimi waste. Kasetsart J (Nat Sci) 43:791-795
93. Scocca J, Lee YC (1969) The composition and structure of the carbohydrate of pineapple stem bromelain. J Biol Chem244(18):4852-4863
94. Seo WH, Lee HG, Baek HH (2008) Evaluation of bitterness in enzymatic hydrolysates of soy protein isolate by taste dilution analysis. J Food Sci 73(1):41-46. doi:10.1111/j.1750-3841.2007.00610.x
95. Shian MLP, Baharil NAS, Yu TL, Taher M, Majid FAA Pilot scale extraction of proteolytic enzyme bromelain from pineapple (Ananas comosus). In: 2nd Int Conf Chem Bioprocess Eng in conjunction with (SOMChE 2005), Kota Kinabalu, Sabah., 2005
96. Silva FVD, Santos RLA, Fujiki TL, Leite MS, Fileti AMF (2010) Design of automatic control system for the precipitation of bromelain from the extract of pineapple wastes. Food Sci Technol (Campinas) 30:1033-1040
97. Silveira E, Souza-Jr ME, Santana JCC, Chaves AC, Porto ALF, Tambourgi EB (2009) Expanded bed adsorption of bromelain (E.C.3.4.22.33) from Ananas comosus crude extract. Braz J Chem Eng 26(01):149-157
98. Singh LR, Devi YR, Devi SK (2003) Enzymological characterization of pineapple extract for potential application in oak tasar (Antheraea proylei J.) silk cocoon cooking and reeling. Electron J Biotechnol 6(3):198-207
99. Soares PAG, Coelho D, Mazzola P, Silveira E, Carneiro-da-Cunha MG, Pessoa AJ, Tambourgi E (2011) Studies on bromelain precipitation by ethanol, poly (ethylene glycol) and ammonium sulphate. Chem Eng Trans 24(5):979-984. doi:10.3303/CET1124164
100. Soares PAG, Vaz AFM, Correia MTS, Pessoa A Jr, Carneiro-da-Cunha MG (2012) Purification of bromelain from pineapple wastes by ethanol precipitation. Sep Purif Technol 98:389-395. doi:10.1016/j.seppur.2012.06.042
101. Song M-M, Nie H-L, Zhou Y-T, Zhu L-M, Bao J-Y (2011) Affinity adsorption of bromelain on Reactive Red 120 immobilizedmagnetic composite particles. Sep Sci Technol 46(3):473-482. doi:10.1080/01496395.2010.517594
102. Sonklin C, Laohakunjit N, Kerdchoechuen O (2011) Physicochemical and flavor characteristics of flavoring agent from mungbean protein hydrolyzed by bromelain. J Agric Food Chem 59(15):8475-8483. doi:10.1021/jf202006a
103. Srinath R, Ramalingam C, Nasimun Islam N (2012) Isolation and characterization of bromelain from pineapple (Ananas comosus) and comparing its anti-browning activity on apple juicewith commercial antibrowning agents. Elixir Food Sci 45:7822-7826
104. Staub A, Guillarme D, Schappler J, Veuthey J-L, Rudaz S (2011) Intact protein analysis in the biopharmaceutical field. J Pharm Biomed Anal 55(4):810-822. doi:10.1016/j.jpba.2011.01.031
105. Streiter PJ, Campbell CP, Mandell IB (2012) The effects of skeletal separation and moisture enhancement for improving the eating quality of cull cow beef. Meat Sci 92(4):400-408. doi:10.1016/j.meatsci.2012.05.002
106. Sullivan GA, Calkins CR (2010) Application of exogenous enzymes to beef muscle of high and low-connective tissue. Meat Sci 85(4):730-734. doi:10.1016/j.meatsci.2010.03.033
107. Tanabe S, Arai S, Watanabe M (1996) Modification of wheat flour with bromelain and baking hypoallergenic bread with added ingredients. Biosci, Biotechnol, Biochem 60(8):1269-1272 (Pubitemid 26368091)
108. Tanuja S, Viji P, Zynudheen AA, Joshy CG (2012) Composition, functional properties and antioxidative activity of hydrolysates prepared from the frame meat of Striped catfish (Pangasianodon hypophthalmus). Egypt J Biol 14:27-35
109. Tochi BN, Wang Z, Xu SY, Zhang W (2009) Effect of stem bromelain on the browning of apple juice. Am J Food Technol:1-8
110. Tománková O, Kopečný J (1995) Prediction of feed protein degradation in the rumen with bromelain. Anim Feed Sci Technol 53(1):71-80. doi:10.1016/0377-8401(94)00735-R
111. Umesh Hebbar H, Sumana B, Raghavarao KSMS, Umesh Hebbar H, Sumana B, Raghavarao KSMS (2008) Use of reverse micellar systems for the extraction and purification of bromelain from pineapple wastes. Bioresour Technol 99:4896-4902. doi:10.1016/j.biortech.2007.09.038 (Pubitemid 351503828)
112. Walsh G (2002) Proteins: biochemistry and biotechnology. John Wiley & Sons Ltd, England
113. Watanabe M, Watanabe J, Sonoyama K, Tanabe S (2000) Novel method for producing hypoallergenic wheat flour by enzymatic fragmentation of the constituent allergens and its application to food processing. Biosci, Biotechnol, Biochem 64(12):2663-2667
114. Watts A, Addy M (2001) Tooth discolouration and staining: tooth discolouration and staining: a review of the literature. Br Dent J 190(6):309-316. doi:10.1038/sj.bdj.4800959 (Pubitemid 33755351)
115. Wharton CW (1974) The structure and mechanism of stem bromelain. Biochem J 143:575-586
116. Wheelwright SM (1991) Protein purification. Design and scale up of downstream processing. John Wiley & Sons, Inc
117. Yamada F, Takahashi N, Murachi T (1976) Purification and characterization of a proteinase from pineapple fruit, fruit bromelain FA2. J Biochem 79(6):1223-1234
118. Yasuda Y, Takahashi N, Murachi T (1970) Composition and structure of carbohydrate moiety of stem bromelain. Biochem 9(1):25-32. doi:10.1021/ bi00803a004
119. Yin L, Sun CK, Han X, Xu L, Xu Y, Qi Y, Peng J (2011) Preparative purification of bromelain (EC 3.4.22.33) from pineapple fruit by high-speed counter-current chromatography using a reverse-micelle solvent system. Food Chem 129(3):925-932. doi:10.1016/j.foodchem.2011.05.048
120. Yoshioka S, Izutsu K-i, Aso Y, Takeda Y (1991) Inactivation kinetics of enzyme pharmaceuticals in aqueous solution. Pharm Res 8(4):480-484. doi:10.1023/a:1015899011324
121. Zhang H, Nie H, Yu D, Wu C, Zhang Y, White CJB, Zhu L (2010) Surface modification of electrospun polyacrylonitrile nanofiber towards developing an affinity membrane for bromelain adsorption. Desalin 256(1-3):141-147. doi:10.1016/j.desal.2010.01.026