Partition separation and characterization of the polyhydroxyalkanoates synthase produced from recombinant Escherichia coli using an aqueous two-phase system

Journal of Bioscience and Bioengineering

Volumn 116, Issue 4, 2013, Pages 499-505

  Lan, John Chi Wei ^a   Yeh, Chun Yi ^a   Wang, Chih Chi ^a   Yang, Yu Hsuan ^a   Wu, Ho Shing ^a  

^a YUAN ZE UNIVERSITY   (Taiwan)

Author keywords

Aqueous two phase systems; PHA synthase; Polyhydroxyalkanoates; Recombinant Escherichia coli; Recovery

Indexed keywords

AQUEOUS TWO PHASE SYSTEM; BIODEGRADABLE POLYESTERS; PARTITION COEFFICIENT; PHA SYNTHASES; POLYHYDROXYALKANOATES; PURIFICATION PROCESS; RECOMBINANT ESCHERICHIA COLI; SEPARATION AND CHARACTERIZATIONS;

BACTERIA; ENZYME ACTIVITY; ESCHERICHIA COLI; METAL IONS; RECOVERY; SOLVENT EXTRACTION;

PURIFICATION;

ADENOSINE TRIPHOSPHATE; MACROGOL 6000; METAL ION; POLYHYDROXYALKANOATE SYNTHASE; POTASSIUM DIHYDROGEN PHOSPHATE; SODIUM CHLORIDE; TRACE METAL;

ARTICLE; ENZYME ACTIVITY; ENZYME PURIFICATION; ENZYME SYNTHESIS; ESCHERICHIA COLI; IN VITRO STUDY; NONHUMAN; PH; PROTEIN CONTENT; PROTEIN EXPRESSION;

CUPRIAVIDUS NECATOR; ESCHERICHIA COLI;

AQUEOUS TWO-PHASE SYSTEMS; PHA SYNTHASE; POLYHYDROXYALKANOATES; RECOMBINANT ESCHERICHIA COLI; RECOVERY;

ACYLTRANSFERASES; CHEMICAL FRACTIONATION; CUPRIAVIDUS NECATOR; ESCHERICHIA COLI; HYDROGEN-ION CONCENTRATION; METALS; MOLECULAR WEIGHT; POLYETHYLENE GLYCOLS; POLYHYDROXYALKANOATES; RECOMBINANT PROTEINS; TEMPERATURE; WATER;

EID: 84883279129     PISSN: 13891723     EISSN: 13474421     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2013.04.010     Document Type: Article

References (35)

1. Hazer B., Steinbüchel A. Increased diversification of polyhydroxyalkanoates by modification reactions for industrial and medical applications. Appl. Microbiol. Biotechnol. 2007, 74:1-12.
2. Anderson A.J., Dawes E.A. Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates. Microbiol. Mol. Biol. Rev. 1990, 54:450-472.
3. Sudesh K., Abe H., Doi Y. Synthesis, structure and properties of polyhydroxyalkanoates: biological polyesters. Prog. Polym. Sci. 2000, 25:1503-1555.
4. Qi Q., Steinbüchel A., Rehm B.H.A. In vitro synthesis of poly(3-hydroxydecanoate): purification and enzymatic characterization of type II polyhydroxyalkanoate synthases PhaC1 and PhaC2 from Pseudomonas aeruginosa. Appl. Microbiol. Biotechnol. 2000, 54:37-43.
5. Rehm B.H.A., Qi Q., Beermann B.B., Hinz H.J., Steinbuchel A. Matrix-assisted in vitro refolding of Pseudomonas aeruginosa class II polyhydroxyalkanoate synthase from inclusion bodies produced in recombinant Escherichia coli. Biol. Chem. 2001, 358:263-268.
6. Kichise T., Fukui T., Yoshida Y., Doi Y. Biosynthesis of polyhydroxyalkanoates (PHA) by recombinant Ralstonia eutropha and effects of PHA synthase activity on in vivo PHA biosynthesis. Int. J. Biol. Macromol. 1999, 25:69-77.
7. Jossek R., Reichelt R., Steinbuchel A. In vitro biosynthesis of poly(3-hydroxybutyric acid) by using purified poly(hydroxyalkanoic acid) synthase of Chromatium vinosum. Appl. Microbiol. Biotechnol. 1998, 49:258-266.
8. Rehm B.H.A. Polyester synthases: natural catalysts for plastics. Biol. Chem. 2003, 376:15-33.
9. Thomson N., Roy I., Summers D., Sivaniah E. In vitro production of polyhydroxyalkanoates: achievements and applications. J.Chem. Technol. Biotechnol. 2010, 85:760-767.
10. Liebergesell M., Sonomoto K., Madkour M., Mayer F., Steinbuchel A. purification and characterization of the poly(hydroxyalkanoic acid) synthase from Chromatium vinosum and localization of the enzyme at the surface of poly(hydroxyalkanoic acid) granules. FEBS J. 1994, 226:71-80.
11. Gerngross T.U., Snell K.D., Peoples O.P., Sinskey A.J., Csuhai E., Masamune S., Stubbe J. Overexpression and purification of the soluble polyhydroxyalkanoate synthase from Alcaligenes eutrophus: evidence for a required posttranslational modification for catalytic activity. Biochemistry 1994, 33:9311-9320.
12. Song J.J., Zhang S., Lenz R.W., Goodwin S. In vitro polymerization and copolymerization of 3-hydroxypropionyl-CoA with the PHB synthase from Ralstonia eutropha. Biomacromolecules 2000, 1:433-439.
13. Zhang S., Kolvek S., Goodwin S., Lenz R.W. Poly(hydroxyalkanoic acid) biosynthesis in Ectothiorhodospira shaposhnikovii: characterization and reactivity of a type III PHA synthase. Biomacromolecules 2004, 5:40-48.
14. Tajima K., Tannai H., Satoh Y., Munekata M. Synthesis of poly(3-hydroxybutyrate) by immobilized poly(3-hydroxybutyrate) synthase. Polym. J. 2003, 35:407-410.
15. Tsuge T., Fukui T., Matsusaki H., Taguchi S., Kobayashi G., Ishizaki A., Doi Y. Molecular cloning of two (R)-specific enoyl-CoA hydratase genes from Pseudomonas aeruginosa and their use for polyhydroxyalkanoate synthesis. FEMS Microbiol. Lett. 2000, 184:193-198.
16. Albertsson P.A. partition of cell particles and macromolecules 1986, Wiley, New York. 3rd ed.
17. Ooi C.W., Tey B.T., Hii S.L., Kamal S.M.M., Lan J.C.W., Ariff A., Ling T.C. Purification of lipase derived from Burkholderia pseudomallei with alcohol/salt-based aqueous two-phase systems. Process Biochem. 2009, 44:1083-1087.
18. Ooi C.W., Hii S.L., Kamal S.M.M., Ariff A., Ling T.C. Extractive fermentation using aqueous two-phase systems for integrated production and purification of extracellular lipase derived from Burkholderia pseudomallei. Process Biochem. 2011, 46:68-73.
19. Hatti-Kaul R. Aqueous two-phase systems, methods in biotechnology 2000, Humana Press Inc., Totowa, New York.
20. Rito-Palomares M. Practical application of aqueous two-phase partition to process development for the recovery of biological products. J.Chromatogr. B 2004, 807:3-11.
21. Hiraishi T., Kikkawa Y., Fujita M., Normi Y.M., Kanesato M., Tsuge T., Sudesh K., Maeda M., Doi Y. Atomic force microscopic observation of in vitro polymerized poly[(R)-3-hydroxybutyrate]: insight into possible mechanism of granule formation. Biomacromolecules 2005, 6:2671-2677.
22. Spiekermann P., Rehm B.H.A., Kalscheuer R., Baumeister D., Steinbüchel A. Asensitive, viable-colony staining method using Nile red for direct screening of bacteria that accumulate polyhydroxyalkanoic acids and other lipid storage compounds. Arch. Microbiol. 1999, 171:73-80.
23. Satoh Y., Tajima K., Tannai H., Munekata M. Enzyme-catalyzed poly(3-hydroxybutyrate) synthesis from acetate with CoA recycling and NADPH regeneration in vitro. J.Biosci. Bioeng. 2003, 95:335-341.
24. Bradford M.M. Arapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976, 72:248-254.
25. Lan C.W., Ling T.C., O'Sullivan D.A., Lyddiatt A. Comparison of two matrices for selective recovery of C595 diabody fragment (dbFv) from Escherichia coli lysates. Process Biochem. 2007, 42:335-343.
26. Porto T.S., Medeiros-Silva G.M., Porto C.S., Cavalcanti M.T.H., Neto B.B., Lima-Filho J.L., Converti A., Porto A.L.F., Pessoa A. Liquid-liquid extraction of proteases from fermented broth by PEG/citrate aqueous two-phase system. Chem. Eng. Process. 2008, 47:716-721.
27. Antonio R.V., Steinbüchel A., Rehm B.H.A. Analysis of in vivo substrate specificity of PHA synthase from Ralstonia eutropha: formation of novel copolyesters in recombinant Escherichia coli. FEMS Microbiol. Lett. 2000, 182:111-117.
28. Huddleston J.G., Ottomar K.W., Ngonyani D.M., Lyddiatt A. Influence of system and molecular parameters upon fractionation of intracellular proteins from Saccharomyces by aqueous two-phase partition. Enzyme Microb. Technol. 1991, 13:24-32.
29. Tanuja S., Srinivas N.D., Raghava Rao K.S.M.S., Gowthaman M.K. Aqueous two-phase extraction for downstream processing of amyloglucosidase. Process Biochem. 1997, 32:635-641.
30. Lima A.S., Alegre R.M., Meirelles A.J.A. Partitioning of pectinolytic enzymes in polyethylene glycol/potassium phosphate aqueous two-phase systems. Carbohydr. Polym. 2002, 50:63-68.
31. Johansson G. Partition of salts and their effects on partition of proteins in a dextran-poly(ethylene glycol)-water two-phase system. Biochim. Biophys. Acta 1970, 221:387-390.
32. De Sourza R.L., Barbosa J.M.P., Zanin G.M., Lobão M.W., Soares C.M.F., Lima A.S. Partitioning of porcine pancreatic lipase in a two-phase systems of polyethylene glycol/potassium phosphate aqueous. Appl. Biochem. Biotechnol. 2010, 161:288-300.
33. Forciniti D. Studying the influence of salts on partitioning of proteins. Methods in biotechnology, vol. 11. Aqueous two phase system: methods and protocols 2000, 23-33. Humana Press Inc., Totowa, NJ, USA. R.H. Kaul (Ed.).
34. Nucci H.D., Nerli B., Pico G. Comparison between the thermodynamic features of α 1-antitrypsin and human albumin partitioning in aqueous two-phase systems of polyethyleneglycol-dextran. Biophys. Chem. 2001, 89:219-229.
35. Zhang S., Yauso T., Lenz R.W., Goodwin S. Kinetic and mechanistic characterization of the polyhydroxybutyrate synthase from Ralstonia eutropha. Biomacromolecules 2000, 1:244-251.