Effect of polyols on polyethylene glycol (PEG)-induced precipitation of proteins: Impact on solubility, stability and conformation

International Journal of Pharmaceutics

Volumn 366, Issue 1-2, 2009, Pages 38-43

  Kumar, Vineet ^a   Sharma, Vikas K ^b   Kalonia, Devendra S ^a  

^a UNIVERSITY OF CONNECTICUT   (United States)

^b GENENTECH INC   (United States)

Author keywords

Polyethylene glycol (PEG); Polyol; Precipitation; Proteins; Secondary structure

Indexed keywords

BOVINE SERUM ALBUMIN; ETHYLENE GLYCOL; GLUCOSE; GLYCEROL; MACROGOL; MACROGOL 400; MACROGOL 8000; POLYOL; RAFFINOSE; SORBITOL; SUCROSE;

ARTICLE; PRECIPITATION; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN STABILITY; SOLUBILITY; THERMOSTABILITY;

ANIMALS; CALORIMETRY, DIFFERENTIAL SCANNING; CATTLE; CIRCULAR DICHROISM; DRUG STABILITY; MOLECULAR WEIGHT; POLYETHYLENE GLYCOLS; PRECIPITATION; PROTEIN STRUCTURE, SECONDARY; SERUM ALBUMIN, BOVINE; SOLUBILITY; SUGAR ALCOHOLS; THERMODYNAMICS;

EID: 57849148494     PISSN: 03785173     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ijpharm.2008.08.037     Document Type: Article

References (19)

1. Arakawa T., and Timasheff S.N. Mechanism of poly(ethyelene glycol) interaction of proteins. Biochemistry 24 (1985) 6756-6762
2. Atha D.H., and Ingham K.C. Mechanism of precipitation of proteins by polyethylene glycols: analysis in terms of excluded volume. J. Biol. Chem. 256 (1981) 12108-12117
3. Back J.F., Oakenfull D., and Smith M.B. Increased thermal stability of proteins in the presence of sugars and polyols. Biochemistry 18 (1979) 5191-5196
4. Bhat R., and Timasheff S.N. Steric exclusion is the principal source of the preferential hydration of proteins in the presence of polyethylene glycols. Protein Sci. 1 (1992) 1133-1143
5. Farrugia B., Nerli B., DiNuci H., Rigatusso R., and Pico G. Thermal features of bovine serum albumin unfolding by polyethylene glycols. Int. J. Biol. Macromol. 26 (1999) 23-33
6. Farrugia B., Garcia G., D'Angelo C., and Pico G. Destabilization of human serum albumin by polyethylene glycols as studied by thermodynamic equilibrium and kinetic approaches. Int. J. Biol. Macromol. 20 (1997) 43-51
7. Francis G.E., Fisher D., Delgado C., Malik F., Gardiner A., and Neale D. PEGylation of cytokines and other therapeutic proteins and peptides. Int. J. Pharm. 68 (1998) 1-18
8. Gekko K., and Timasheff S.N. Thermodynamic and kinetic examination of protein stabilization by glycerol. Biochemistry 20 (1981) 4677-4686
9. Gekko K., and Timasheff S.N. Mechanism of protein stabilization by glycerol: preferential hydration of glycerol-water mixtures. Biochemistry 20 (1981) 4667-4676
10. Gilliland G.F., and Davies D.R. Protein crystallization: the growth of large scale single crystals. Methods Enzymol. 104 (1984) 370-380
11. Kendrick B.S., Chang B.S., Arakawa T., Peterson B., Randolph T.W., Manning M., and Carpenter J.F. Preferential exclusion of sucrose from interleukin-1 receptor antagonist: role in restricted conformational mobility and compaction of native state. Proc. Natl. Acad. Sci. 94 (1997) 11917-11922
12. Lee J.C., and Timasheff S.N. The stabilization of proteins by sucrose. J. Biol. Chem. 256 (1981) 7193-7201
13. McClements J.D. Modulation of globular protein functionality by weakly interacting co-solvents. Crit. Rev. Food Sci. 42 (2002) 417-471
14. McPherson A. Use of polyethylene glycol in the crystallization of macromolecules. Methods Enzymol. 114 (1985) 120-124
15. Paleg L.G., Stewart G.R., and Bradbeer J.W. Proline and glycine betaine influence protein solvation. Plant Physiol. 75 (1984) 974-978
16. Sharma V.K., and Kalonia D.S. Polyethylene glycol-induced precipitation of interferon alpha-2a followed by vacuum drying: development of a novel process for obtaining a dry stable powder. AAPS PharmSci. 6 (2004) 1-14 (Artilce-4)
17. Winholz M., Budavari S., Stroumatsos L.Y., and Fertig M.N. Polyethylene glycol. The Merck index (1976), Merck and Co. Inc., Whitehouse Station, NJ pp. 983-984
18. Winzor C.L., Winzor D.J., Paleg L.G., Jones G.P., and Naidu B.P. Rationalization of the effect of compatible solutes on protein stability in terms of thermodynamic non ideality. Arch. Biochem. Biophys. 296 (1992) 102-107
19. Xie G., and Timasheff S.N. Mechanism of the stabilization of ribonuclease A by sorbitol: preferential hydration is greater for the unfolded state than for the native protein. Protein Sci. 6 (1997) 211-221