Extraction of bromelain from pineapple core and purification by RME and precipitation methods

Separation and Purification Technology

Volumn 111, Issue , 2013, Pages 90-97

  Chaurasiya, Ram Saran ^a   Umesh Hebbar, H ^a  

^a ACADEMY OF SCIENTIFIC AND INNOVATIVE RESEARCH ACSIR   (India)

Author keywords

Bromelain; ineapple core; Precipitation; Purification; RME

Indexed keywords

EXTRACTION CONDITIONS; INEAPPLE CORE; LIQUID-LIQUID EXTRACTION; PRECIPITATION METHODS; PRECIPITATION TECHNIQUES; REVERSE MICELLAR EXTRACTIONS; RME; SEPARATION AND PURIFICATION;

ACETONE; EXTRACTION; FRUITS; LASERS; OPTIMIZATION; PRECIPITATION (CHEMICAL); PURIFICATION;

BROMELAIN;

ANANAS COMOSUS;

EID: 84876245391     PISSN: 13835866     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.seppur.2013.03.029     Document Type: Article

References (46)

1. A.D. Rowan, D.J. Buttle, and A.J. Barrett The cysteine proteinases of the pineapple plant Biochem. J. 266 1990 869 875
2. A. Jeung Encyclopedeia of Common Natural Ingredients Used in Foods, Drugs, and Cosmetics 1980 John Wiley & Sons New York pp. 74-76
3. B.K. Bhattacharyya Bromelain - an overview Nat. Prod. Radian. 7 2008 359 363
4. J. Choe, A.A. Vandernoot, R.J. Linhardt, and J.S. Dordick Resolution of glycoproteins by affinity-based reversed micellar extraction and separation AIChE J. 44 1998 2542 2548
5. H.R. Maurer Bromelain: biochemistry, pharmacology and medical use Cell. Mol. Life Sci. 58 2001 1231 1245
6. W.W. Eckenfelder, J.P. Lawler, J.T. Walsh, Study of fruit and vegetable processing waste disposal methods in eastern regions, US Department of Agriculture, final report, New York state canners associates, 1958.
7. A.D. Diamond, and J.T. Hsu Aqueous two phase systems for biomolecule separation Adv. Biochem. Eng. Biotechnol. 47 1992 89 135
8. K.S.M.S. Raghavarao, M.R. Guinn, and P.W. Todd Recent developments in aqueous two-phase extraction in bioprocessing Sep. Purif. Methods 27 1998 1 50
9. E.N. Lightfoot, Protein purification from molecular mechanisms to large scale processes, In: M.R. Ladisch, R.C. Willson, C.C. Painton, S.E. Builder (Eds.), Separation in biotechnology - the key role of adsorptive separations, ACS Publications, New York, vol. 427, 1990, pp. 35-51.
10. K.E. Goklen, and T.A. Hatton Liquid-liquid extraction of low molecular weight proteins by selective solubilization in reversed micelles Sep. Sci. Technol. 22 1987 831 841
11. M. Dekker, K.V. Riet, B.H. Bijsterbosch, R.B.G. Wolbert, and R. Hilhorst Modeling and optimization of the reversed micellar extraction of α - amylase AIChE J. 35 1989 321 324
12. U.H. Hebbar, A.B. Hemavathi, B. Sumana, and K.S.M.S. Raghavrao Reverse micellar extraction of bromelain from pineapple (Ananas comosus L. Merryl) waste: scale-up, reverse micelles characterization and mass transfer studies Sep. Sci. Technol. 46 2011 1656 1664
13. U.H. Hebbar, B. Sumana, A.B. Hemavathi, and K.S.M.S. Raghavarao Separation and purification of bromelain by reverse micellar extraction coupled ultrafiltration and comparative studies with other methods Food Bioprocess Technol. 5 2010 1010 1018
14. U.H. Hebbar, B. Sumana, and K.S.M.S. Raghavarao Use of reverse micellar systems for the extraction and purification of bromelain from pineapple wastes Bioresour. Technol. 99 2008 4896 4902
15. U.H. Hebbar, and K.S.M.S. Raghavarao Extraction of bovine serum albumin using nanoparticulate reverse micelles Process Biochem. 42 2007 1602 1608
16. A.B. Hemavathi, U.H. Hebbar, and K.S.M.S. Raghavarao Mixed reverse micellar systems for extraction and purification of β-glucosidase Sep. Purif. Technol. 71 2010 263 268
17. A.B. Hemavathi, U.H. Hebbar, and K.S.M.S. Raghavarao Reverse micellar extraction of β-galactosidase from barley (Hordeum vulgare) Appl. Biochem. Biotechnol. 151 2008 522 531
18. A.B. Hemavathi, U.H. Hebbar, and K.S.M.S. Raghavarao Reverse micellar extraction of bromelain from Ananas comosus L. Merryl J. Chem. Technol. Biotechnol. 82 2007 985 992
19. J.G. Liu, J.M. Xing, R. Shen, C.L. Yang, and H.Z. Liu Reverse micellar extraction of nattokinase from fermentation broth Biochem. Eng. J. 21 2004 273 278
20. M.E. Raikar, D. Hayes, and J.M. Harris Solubilization of enzymes in water-in-oil microemulsions and their rapid and efficient release through use of a pH-degradable surfactant Biotechnol. Lett. 29 2007 767 771
21. Y.O. Shin, M.E. Webber, and J.H. Vera Effect of salt and volume ratio on the reverse micellar extraction of lysozyme using DODMAC Fluid Phase Equilib. 207 2003 155 165
22. W. Zhang, H. Liu, and J. Chen Forward and backward extraction of BSA using mixed reverse micellar system of CTAB and alkyl halides Biochem. Eng. J. 12 2002 1 5
23. S. Englard, and S. Seifter Precipitation Techniques M.P. Deutscher, Guide to Protein Purification 1990 Academic Press San Diego, USA
24. S. Ota, M. Stanford, and W.H. Stein Preparation and chemical properties of purified stem and fruit bromelains Biochemistry 3 1964 180 185
25. E. Layne Spectrophotometric and turbidimetric methods for measuring proteins Methods Enzymol. 3 1957 447 455
26. T. Murachi Bromelain Enzymes L. Lorand, Methods in Enzymology 1976 Academic Press New York, XLV 475 485
27. J.R. Das, S.G. Bhat, and L.R. Gowda Purification and characterization of polyphenol oxidase from the Kew cultivar of Indian pineapple fruit J. Agric. Food Chem. 45 1997 2031 2035
28. C.P. Malik, and M.B. Singh Plant Enzymology and Histoenzymology 1980 Kalyani Publishers New Delhi
29. J. Putter Methods of Enzymatic Analysis second ed. 1974 Academic Press New York, Bergmeyer pp. 685-689
30. AOAC, Fruits and fruits products. In: K. Helrich (Ed.), Official Methods of Analysis, Association of Official Analytical Chemists, Arlington, USA, 1990, pp. 910-928.
31. C.S. James, Analytical Chemistry of Foods, Chapman and Hall, New York, vol. 138, 1995, pp. 96-97.
32. M. Dubois, K.A. Gilles, J.K. Hamilton, P.A. Rebers, and F. Smith Colorimetric method for determination of sugars and related substances Anal. Chem. 28 1956 350 354
33. U.K. Laemmli Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227 1970 680 685
34. B.R. Oakley, D.R. Kirsch, and N.R. Morris A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels Anal. Biochem. 105 1980 361 363
35. A. Gordon, and S. Barbut Effect of chloride salts on protein extraction and interfacial protein film formation in meat batters J. Sci. Food Agric. 58 2006 227 238
36. T. Murachi, and M. Yamazaki Changes in conformation and enzymatic activity of stem bromelain in alkaline media Biochemistry 9 1970 1935 1938
37. J.R. Whitaker, Principles of Enzymology for the Food Sciences, Fennema, O.R. Marcel dekkar, Inc., New York, vol. 79, 1972.
38. J.F. Morton Pineapple J.F. Morton, Fruits of Warm Climate 1987 Florida Flair Books Miami, FL 18 28
39. Y.M. Chen, and H.Y. Liu Studies on stem bromelain and stem starch from pineapple plants Taiwania 17 1972 266 276
40. A.M.F. Fileti, G.A. Fischer, J.C.C. Santana, and A.B. Tambourgi Batch and continuous extraction of bromelain enzyme by reversed micelles Braz. Arch. Biol. Technol. 52 2009 1225 1234
41. S.Y. Huang, and Y.C. Lee Separation and purification of horseradish peroxidase from Armoracia rusticana root using reversed micellar extraction Bioseparation 4 1994 1 5
42. V.M. Paradkar, and J.S. Dordick Affinity based reverse micellar extraction and separation (ARMES): a facile technique for the purification of peroxidase from soybean hulls Biotechnol. Prog. 9 1993 199 203
43. A. Narayan, M. Madhusudhan, and K. Raghavarao Extraction and purification of ipomoea peroxidase employing three-phase partitioning Appl. Biochem. Biotechnol. 151 2008 263 272
44. R.V. Devakate, V.V. Patil, S.S. Waje, and B.N. Thorat Purification and drying of bromelain Sep. Purif. Technol. 64 2009 259 264
45. M.B. Doko, V. Bassani, J. Casadebaig, L. Cavailles, and M. Jacob Preparation of proteolytic enzyme extracts from Ananas comosus L., Merr. Fruit juice using semipermeable membrane, ammonium sulfate extraction, centrifugation and freeze-drying processes Int. J. Pharm. 76 1991 199 206
46. A.P.B. Rabelo, E.B. Tambourgi, and J.A. Pessoa Bromelain partitioning in two phase aqueous system consisting PEO-PPO-PEO block copolymers J. Chromatogr. B 807 2004 61 68