Bromelain partitioning in two-phase aqueous systems containing PEO-PPO-PEO block copolymers

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volumn 807, Issue 1, 2004, Pages 61-68

  Rabelo, Ana Paula Brescancini ^a   Tambourgi, Elias Basile ^b   Pessoa Jr , Adalberto ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

^b UNIVERSITY OF CAMPINAS   (Brazil)

Author keywords

Aqueous two phase systems; Bromelain; Enzyme purification; Partitioning; Thermoseparation

Indexed keywords

CONCENTRATION (PROCESS); ENZYMES; PH EFFECTS; PURIFICATION; SEPARATION; SOLUTIONS;

BUFFER CONCENTRATION; MOLECULAR MASS; PARTITION COEFFICIENT; THERMOSEPARATION;

COPOLYMERS;

BROMELAIN; COPOLYMER; MACROGOL; POLYPROPYLENE; SODIUM CHLORIDE; VEGETABLE PROTEIN;

ALKALINITY; AQUEOUS SOLUTION; CONCENTRATION PROCESS; CONFERENCE PAPER; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME ISOLATION; ENZYME PURIFICATION; HIGH TEMPERATURE; MOLECULAR WEIGHT; PARTITION CHROMATOGRAPHY; PARTITION COEFFICIENT; PINEAPPLE; PRIORITY JOURNAL; TEMPERATURE DEPENDENCE; THERMODYNAMICS;

BROMELAINS; HYDROGEN-ION CONCENTRATION; MOLECULAR WEIGHT; POLYETHYLENE GLYCOLS; POLYMERS; POLYPROPYLENES; TEMPERATURE;

ANANAS COMOSUS;

EID: 2642511370     PISSN: 15700232     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jchromb.2004.03.029     Document Type: Conference Paper

References (21)

1. D.E. Brooks, H. Walter, D. Fisher, Partitioning in Aqueous Two Phase Systems, Academic Press, Orlando, 1985.
2. P.A. Albertsson, Partition of Cell Particles and Macromolecules, third ed., Wiley/Interscience, New York, 1986, 323 pp.
3. H. Walter, G. Johansson, Methods in Enzymology, vol. 228, Academic Press, Londres, 1994, pp. 600-608.
4. Alred P.A., Tjerneld F., Koslowski A., Harris J.M. Bioseparation. 2:1992;363
5. Franco T.T., Galaev Y.U., Hatti-Kaul R., Holberg N., Bülow L., Mattiasson B. Biotechnol. Tech. 11:1997;231
6. Persson J., Johansson H.-O., Tjerneld F. J. Chromatogr. A. 864:1999;31
7. Persson J., Nystrom L., Ageland H., Tjerneld F. J. Chem. Technol. Biotechnol. 74:1999;238
8. Alexandridis P., Hatton T.A. Colloids Surf. A: Physicochem. Eng. Aspects. 96:1995;1
9. Hvidt S. Colloids Surf. A: Physicochem. Eng. Aspects. 112:1995;201
10. Persson J., Kaul A., Tjerneld F. J. Chromatogr. B. 743:2000;115
11. Berggren K., Johansson H.-O., Tjerneld F. J. Chromatogr. A. 718:1995;67
12. Saitoh T., Tani H., Kamidate T., Watanabe H. Trends Anal. Chem. 14:1995;213
13. Cunha M.T., Tjerneld F., Cabral J.M.S., Aires-Barros M.R. J. Chromatogr. B. 711:1998;53
14. Feinstein G., Whitaker J.R. Biochemistry. 3:1964;1050
15. T. Murachi, Bromelain enzymes, in: L. Lorand (Ed.), Methods in Enzymology, vol. XLV, Academic Press, New York, 1976, pp. 475-485.
16. Arroyo-Reyna A., Hernández-Arana A. Biochim. Biophys. Acta. 1248:1995;123
17. Bradford M.M. Anal. Biochem. 72:1976;248
18. A.C.W. Cesar, R. Silva, A.C. Lucarini, RIC, São Carlos, SP, Brazil 01 (1999) 47-54.
19. Baldini V.L.S., Iaderoza M., Ferreira E.A.H., Sales A.M., Draetta I.S., Giacomelli E.J. Colet. ITAL, Campinas. 23:1993;44
20. Karlstron G. J. Phys. Chem. 89:1985;4962
21. Alred P.A., Koslowski A., Harris J.M., Tjerneld F. J. Chromatogr. A. 659:1994;289