Expression, purification, and characterization of a recombinant stem bromelain from Ananas comosus

Process Biochemistry

Volumn 46, Issue 12, 2011, Pages 2232-2239

  Amid, Azura ^a   Ismail, Nurul Azira ^a   Yusof, Faridah ^a   Salleh, Hamzah Mohd ^a  

^a INTERNATIONAL ISLAMIC UNIVERSITY MALAYSIA   (Malaysia)

Author keywords

Bromelain; Cloning; Protease; Protein expression; Recombinant enzyme

Indexed keywords

ANANAS COMOSUS; BROMELAIN; DNA SEQUENCING; EXPRESSION VECTORS; HYDROLYTIC ACTIVITIES; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; OPTIMUM ACTIVITY; PROTEASE; PROTEIN EXPRESSION; PROTEIN EXPRESSIONS; PUBLIC DATABASE; PURIFICATION METHOD; PURIFIED ENZYME; RECOMBINANT ENZYMES; SINGLE-STEP; SPIN COLUMN; SYNTHETIC SUBSTRATES; WESTERN BLOTTING;

AFFINITY CHROMATOGRAPHY; CLONING; COLUMN CHROMATOGRAPHY; DNA SEQUENCES; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE ENCODING; POLYMERASE CHAIN REACTION; PURIFICATION;

GENE EXPRESSION;

ANANAS COMOSUS; ESCHERICHIA COLI;

EID: 80054968109     PISSN: 13595113     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.procbio.2011.08.018     Document Type: Article

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