Impacts of high β-galactosidase expression on central metabolism of recombinant Pichia pastoris GS115 using glucose as sole carbon source via 13C metabolic flux analysis

Journal of Biotechnology

Volumn 187, Issue , 2014, Pages 124-134

  Nie, Yongsheng ^a   Huang, Mingzhi ^a   Lu, Junjie ^a   Qian, Jiangchao ^a   Lin, Weilu ^a   Chu, Ju ^a   Zhuang, Yingping ^a   Zhang, Siliang ^a  

^a EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Central metabolism; Heterologous protein expression; P. pastoris

Indexed keywords

CARBON; GLUCOSE; OPTIMIZATION; OXIDATION; PHYSIOLOGY; PROTEINS; YEAST;

CENTRAL METABOLISMS; CONSTITUTIVE PROMOTERS; HETEROLOGOUS PROTEINS; METABOLIC FLUX ANALYSIS; MICROBIAL CELL FACTORIES; P. PASTORIS; PENTOSE PHOSPHATE PATHWAY; PICHIA PASTORIS GS115; GALACTOSIDASES; HIGH BETA; SOLE CARBON SOURCE;

METABOLISM;

BETA GALACTOSIDASE; CARBON 13; GLUCOSE; ADENOSINE TRIPHOSPHATE; CARBON; NICOTINAMIDE ADENINE DINUCLEOTIDE; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; RECOMBINANT PROTEIN;

ARTICLE; CARBON SOURCE; CITRIC ACID CYCLE; ENERGY METABOLISM; FUNGAL STRAIN; GLYCOLYSIS; GROWTH RATE; METABOLIC FLUX ANALYSIS; NONHUMAN; PENTOSE PHOSPHATE CYCLE; PICHIA PASTORIS; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROMOTER REGION; PROTEIN EXPRESSION; SECRETORY PATHWAY; ENZYMOLOGY; GENETICS; METABOLIC ENGINEERING; METABOLISM; PICHIA; PROCEDURES; FUNGAL METABOLISM;

ADENOSINE TRIPHOSPHATE; BETA-GALACTOSIDASE; CARBON ISOTOPES; GLUCOSE; METABOLIC ENGINEERING; METABOLIC FLUX ANALYSIS; NAD; NADP; PICHIA; RECOMBINANT PROTEINS;

EID: 84906352291     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2014.07.011     Document Type: Article

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