Carbon metabolism limits recombinant protein production in Pichia pastoris

Biotechnology and Bioengineering

Volumn 108, Issue 8, 2011, Pages 1942-1953

  Heyland, Jan ^a   Fu, Jianan ^a   Blank, Lars M ^a   Schmid, Andreas ^a,b  

^a TU DORTMUND UNIVERSITY   (Germany)

^b LEIBNIZ INSTITUT FÜR ANALYTISCHE WISSENSCHAFTEN ISAS E V   (Germany)

Author keywords

13C flux analysis; Amino acid biosynthetic cost; Crabtree; Metabolic network analysis; Off gas analysis; Yeast

Indexed keywords

AMINO PEPTIDASE; BY-PRODUCT FORMATION; CARBON METABOLISM; CELL PHYSIOLOGY; CELLULAR METABOLISM; CRABTREE; ENERGY GENERATIONS; FERMENTATION MEDIUM; FLUX ANALYSIS; GROWTH MEDIUM; LIMITING FACTORS; LOW-TO-HIGH; METABOLIC NETWORK; METABOLIC NETWORK ANALYSIS; MODEL PROTEINS; MOLECULAR TOOLS; OFF-GAS ANALYSIS; P. PASTORIS; PICHIA PASTORIS; PRODUCTION YIELD; PROTEIN PRODUCTION; PROTEINOGENIC AMINO ACIDS; RECOMBINANT PROTEIN; RECOMBINANT PROTEIN PRODUCTIONS; REDUCED GROWTH RATE; TCA CYCLE; UPPER LIMITS;

AMINO ACIDS; BIOSYNTHESIS; CYTOLOGY; ELECTRIC NETWORK ANALYSIS; FERMENTATION; GENES; METABOLISM; OPTIMIZATION; PHYSIOLOGY; TRANSCRIPTION; YEAST;

PROTEINS;

AMINO ACID; FUNGAL PROTEIN; FUNGAL PROTEIN BAPA; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

AMINO ACID SYNTHESIS; ARTICLE; CARBON METABOLISM; CELL FUNCTION; CELL METABOLISM; CITRIC ACID CYCLE; ENERGY METABOLISM; FERMENTATION; FUNGAL BIOMASS; FUNGAL STRAIN; FUNGUS; FUNGUS GROWTH; GROWTH RATE; NONHUMAN; PICHIA PASTORIS; PROTEIN SYNTHESIS; SPHINGOSINICELLA XENOPEPTIDILYTICA;

AMINO ACIDS; AMINOPEPTIDASES; CARBON; CULTURE MEDIA; ENERGY METABOLISM; PICHIA; RECOMBINANT PROTEINS; SPHINGOMONADACEAE;

PICHIA PASTORIS; SPHINGOSINICELLA;

EID: 79959227594     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.23114     Document Type: Article

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