Quantification of metabolic limitations during recombinant protein production in Escherichia coli

Journal of Biotechnology

Volumn 155, Issue 2, 2011, Pages 178-184

  Heyland, Jan ^a   Blank, Lars M ^a   Schmid, Andreas ^a,b  

^a TU DORTMUND UNIVERSITY   (Germany)

^b LEIBNIZ INSTITUT FÜR ANALYTISCHE WISSENSCHAFTEN ISAS E V   (Germany)

Author keywords

13C flux analysis; Flux probe; Metabolic network analysis; MFA; Recombinant protein production

Indexed keywords

ATP GENERATION; BATCH CULTIVATION; BIOMASS YIELD; CELLULAR ENERGY; E. COLI; ELECTRON TRANSPORT CHAIN; ENERGY DEMANDS; EXPRESSION VECTORS; FLUX ANALYSIS; FLUX DISTRIBUTIONS; GENERATION RATE; METABOLIC FLUX ANALYSIS; METABOLIC NETWORK ANALYSIS; MFA; MOLECULAR GENETICS; RECOMBINANT PROTEIN; RECOMBINANT PROTEIN PRODUCTIONS; REFERENCE STRAINS; SOLUBLE TRANSHYDROGENASE; TCA CYCLE;

BIOSYNTHESIS; CELL CULTURE; CHARGE DENSITY WAVES; ELECTRIC NETWORK ANALYSIS; ENERGY MANAGEMENT; ESCHERICHIA COLI; GENE EXPRESSION; METABOLISM; MOLECULAR BIOLOGY; PHYSIOLOGY; PROBES; STRAIN;

PROTEINS;

ADENOSINE TRIPHOSPHATE; NICOTINAMIDE ADENINE DINUCLEOTIDE (PHOSPHATE) TRANSHYDROGENASE; RECOMBINANT PROTEIN; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; TRACER;

ARTICLE; BACTERIAL STRAIN; BIOMASS PRODUCTION; CELLULAR DISTRIBUTION; CITRIC ACID CYCLE; CONTROLLED STUDY; CULTURE TECHNIQUE; ELECTRON TRANSPORT; ENERGY; ESCHERICHIA COLI; GENE EXPRESSION; NONHUMAN; PRIORITY JOURNAL; PROTEIN METABOLISM; PROTEIN SYNTHESIS; QUANTITATIVE ANALYSIS;

ANILINE COMPOUNDS; BIOTECHNOLOGY; CARBON ISOTOPES; CELL CULTURE TECHNIQUES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; METABOLIC NETWORKS AND PATHWAYS; RECOMBINANT PROTEINS; SPECTROPHOTOMETRY;

ESCHERICHIA COLI;

EID: 80051474345     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2011.06.016     Document Type: Article

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