13C-metabolic flux analysis in heterologous cellulase production by Bacillus subtilis genome-reduced strain

Journal of Biotechnology

Volumn 179, Issue 1, 2014, Pages 42-49

  Toya, Yoshihiro ^a,b   Hirasawa, Takashi ^a,b,c   Morimoto, Takuya ^b,d   Masuda, Kenta ^b,d   Kageyama, Yasushi ^b,d   Ozaki, Katsuya ^b,d   Ogasawara, Naotake ^b,e   Shimizu, Hiroshi ^a,b  

^a OSAKA UNIVERSITY   (Japan)

^b JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

^c TOKYO INSTITUTE OF TECHNOLOGY   (Japan)

^d KAO CORPORATION   (Japan)

^e NARA INSTITUTE OF SCIENCE AND TECHNOLOGY   (Japan)

Author keywords

Bacillus subtilis; Cellulase production; Genome reduced strain

Indexed keywords

BIOLOGICAL MATERIALS; GLUCOSE; METABOLISM; PHYSIOLOGY;

BACILLUS SUBTILIS; CELLULASE PRODUCTION; CITRATE SYNTHASE; FLUX ANALYSIS; GLYCOLYTIC INTERMEDIATE; PENTOSE PHOSPHATE PATHWAY; SYNTHETIC MEDIUM; WILD-TYPE STRAIN;

GENES;

2 OXOGLUTARIC ACID; ACETYL COENZYME A; CARBON 13; CELLULASE; CITRATE SYNTHASE; GLUCOSE; GLUTAMIC ACID; PENTOSE PHOSPHATE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; BACTERIAL PROTEIN; CARBON; CULTURE MEDIUM; EGL-237 PROTEIN, BACILLUS;

ARTICLE; BACILLUS SUBTILIS; BACTERIAL GENOME; BACTERIAL STRAIN; CARBON METABOLISM; CARBON SOURCE; CELL GROWTH; CELL METABOLISM; CONTROLLED STUDY; GENE DELETION; GLYCOLYSIS; METABOLIC ENGINEERING; METABOLIC FLUX ANALYSIS; NONHUMAN; PRIORITY JOURNAL; BIOSYNTHESIS; CHEMISTRY; CLASSIFICATION; CULTURE MEDIUM; ESSENTIAL GENE; GENE EXPRESSION REGULATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLISM; PENTOSE PHOSPHATE CYCLE;

BACILLUS SUBTILIS; BACTERIAL PROTEINS; CARBON ISOTOPES; CELLULASE; CITRATE (SI)-SYNTHASE; CULTURE MEDIA; GENE DELETION; GENE EXPRESSION REGULATION, BACTERIAL; GENES, ESSENTIAL; GENOME, BACTERIAL; GLUCOSE; GLUTAMIC ACID; GLYCOLYSIS; PENTOSE PHOSPHATE PATHWAY;

EID: 84897433019     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2014.03.025     Document Type: Article

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