A quantitative study of methanol/sorbitol co-feeding process of a Pichia pastoris Mut+/pAOX1-lacZ strain

Microbial Cell Factories

Volumn 12, Issue 1, 2013, Pages

  Niu, Hongxing ^a   Jost, Laurent ^b   Pirlot, Nathalie ^b   Sassi, Hosni ^a   Daukandt, Marc ^b   Rodriguez, Christian ^b   Fickers, Patrick ^a  

^a UNIVERSITÉ LIBRE DE BRUXELLES   (Belgium)

^b EUROGENTEC S A   (Belgium)

Author keywords

galactosidase; Fed batch culture; Metabolic flux analysis; Methanol sorbitol co feeding; pAOX1; Pichia pastoris; Transient continuous culture

Indexed keywords

AOX1 PROTEIN; BACTERIAL PROTEIN; BETA GALACTOSIDASE; CARBON DIOXIDE; METHANOL; SORBITOL; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL STRAIN; BACTERIUM CULTURE; CELL GROWTH; CELL METABOLISM; CONTROLLED STUDY; ENZYME SUBSTRATE; FED BATCH CULTURE; NONHUMAN; OXYGEN CONSUMPTION; PICHIA PASTORIS; QUANTITATIVE STUDY; REPORTER GENE; BATCH CELL CULTURE; BIOMASS; GROWTH, DEVELOPMENT AND AGING; METABOLIC FLUX ANALYSIS; METABOLISM; PICHIA;

PICHIA PASTORIS;

BATCH CELL CULTURE TECHNIQUES; BETA-GALACTOSIDASE; BIOMASS; METABOLIC FLUX ANALYSIS; METHANOL; PICHIA; SORBITOL;

EID: 84875870645     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/1475-2859-12-33     Document Type: Article

References (31)

1. Bollok M, Resina D, Valero F, Ferrer P. Recent patents on the Pichia pastoris expression system: expanding the toolbox for recombinant protein production. Recent Pat Biotechnol 2009, 3(3):192-201.
2. Cereghino JL, Cregg JM. Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol Rev 2000, 24(1):45-66. 10.1111/j.1574-6976.2000.tb00532.x, 10640598.
3. Daly R, Hearn MT. Expression of heterologous proteins in Pichia pastoris: a useful experimental tool in protein engineering and production. J Mol Recognit 2005, 18(2):119-138. 10.1002/jmr.687, 15565717.
4. De Schutter K, Lin YC, Tiels P, Van Hecke A, Glinka S, Weber-Lehmann J, Rouze P, Van de Peer Y, Callewaert N. Genome sequence of the recombinant protein production host Pichia pastoris. Nat Biotechnol 2009, 27(6):561-566. 10.1038/nbt.1544, 19465926.
5. Hamilton SR, Davidson RC, Sethuraman N, Nett JH, Jiang Y, Rios S, Bobrowicz P, Stadheim TA, Li H, Choi BK, Hopkins D, Wischnewski H, Roser J, Mitchell T, Strawbridge RR, Hoopes J, Wildt S, Gerngross TU. Humanization of yeast to produce complex terminally sialylated glycoproteins. Science 2006, 313(5792):1441-1443. 10.1126/science.1130256, 16960007.
6. Cos O, Ramon R, Montesinos JL, Valero F. Operational strategies, monitoring and control of heterologous protein production in the methylotrophic yeast Pichia pastoris under different promoters: a review. Microb Cell Fact 2006, 5:17. 10.1186/1475-2859-5-17, 1564409, 16600031.
7. Jungo C. Quantitative characterization of a recombinant Pichia pastoris Mut + strain secreting avidin using transient continuous cultures 2007, PhD thesis. EPFL, Laboratory of Chemical and Biological Engineering.
8. Nielsen JH, Villadsen J, Liden G. Bioreaction engineering principles 2003, New York: Kluwer Academic/Plenum Publishers, 2.
9. Zhang W, Hywood Potter KJ, Plantz BA, Schlegel VL, Smith LA, Meagher MM. Pichia pastoris fermentation with mixed-feeds of glycerol and methanol: growth kinetics and production improvement. J Ind Microbiol Biotechnol 2003, 30(4):210-215.
10. Jungo C, Marison I, von Stockar U. Mixed feeds of glycerol and methanol can improve the performance of pichia pastoris cultures: a quantitative study based on concentration gradients in transient continuous cultures. J Biotechnol 2007, 128(4):824-837. 10.1016/j.jbiotec.2006.12.024, 17303281.
11. Zalai D, Dietzsch C, Herwig C, Spadiut O. A dynamic fed batch strategy for a Pichia pastoris mixed feed system to increase process understanding. Biotechnol Prog 2012, 28(3):878-886. 10.1002/btpr.1551, 22505140.
12. Paulova L, Hyka P, Branska B, Melzoch K, Kovar K. Use of a mixture of glucose and methanol as substrates for the production of recombinant trypsinogen in continuous cultures with Pichia pastoris Mut+. J Biotechnol 2012, 157(1):180-188. 10.1016/j.jbiotec.2011.10.010, 22123532.
13. Jorda J, Jouhten P, Camara E, Maaheimo H, Albiol J, Ferrer P. Metabolic flux profiling of recombinant protein secreting Pichia pastoris growing on glucose:methanol mixtures. Microb Cell Fact 2012, 11:57. 10.1186/1475-2859-11-57, 3443025, 22569166.
14. Inan M, Meagher MM. Non-repressing carbon sources for alcohol oxidase (AOX1) promoter of Pichia pastoris. J Biosci Bioeng 2001, 92(6):585-589.
15. Ramon R, Ferrer P, Valero F. Sorbitol co-feeding reduces metabolic burden caused by the overexpression of a Rhizopus oryzae lipase in Pichia pastoris. J Biotechnol 2007, 130(1):39-46. 10.1016/j.jbiotec.2007.02.025, 17399833.
16. Celik E, Calik P, Oliver SG. Fed-batch methanol feeding strategy for recombinant protein production by Pichia pastoris in the presence of co-substrate sorbitol. Yeast 2009, 26(9):473-484. 10.1002/yea.1679, 19575480.
17. Wang Z, Wang Y, Zhang D, Li J, Hua Z, Du G, Chen J. Enhancement of cell viability and alkaline polygalacturonate lyase production by sorbitol co-feeding with methanol in Pichia pastoris fermentation. Bioresour Technol 2010, 101(4):1318-1323. 10.1016/j.biortech.2009.09.025, 19800218.
18. Zhu T, You L, Gong F, Xie M, Xue Y, Li Y, Ma Y. Combinatorial strategy of sorbitol feeding and low-temperature induction leads to high-level production of alkaline beta-mannanase in Pichia pastoris. Enzyme Microb Technol 2011, 49(4):407-412. 10.1016/j.enzmictec.2011.06.022, 22112568.
19. Gao MJ, Li Z, Yu RS, Wu JR, Zheng ZY, Shi ZP, Zhan XB, Lin CC. Methanol/sorbitol co-feeding induction enhanced porcine interferon-alpha production by P. pastoris associated with energy metabolism shift. Bioprocess Biosyst Eng 2012, 35(7):1125-1136. 10.1007/s00449-012-0697-1, 22349926.
20. Jungo C, Schenk J, Pasquier M, Marison IW, von Stockar U. A quantitative analysis of the benefits of mixed feeds of sorbitol and methanol for the production of recombinant avidin with Pichia pastoris. J Biotechnol 2007, 131(1):57-66. 10.1016/j.jbiotec.2007.05.019, 17614154.
21. Celik E, Calik P, Oliver SG. Metabolic flux analysis for recombinant protein production by pichia pastoris using dual carbon sources: effects of methanol feeding rate. Biotech Bioeng 2010, 105(2):317-329. 9.
22. Jungo C, Marison I, von Stockar U. Regulation of alcohol oxidase of a recombinant Pichia pastoris Mut + strain in transient continuous cultures. J Biotechnol 2007, 130(3):236-246. 10.1016/j.jbiotec.2007.04.004, 17566583.
23. Carnicer M, Baumann K, Toplitz I, Sanchez-Ferrando F, Mattanovich D, Ferrer P, Albiol J. Macromolecular and elemental composition analysis and extracellular metabolite balances of Pichia pastoris growing at different oxygen levels. Microb Cell Fact 2009, 8:65. 10.1186/1475-2859-8-65, 2799386, 20003217.
24. Hartner FS, Glieder A. Regulation of methanol utilisation pathway genes in yeasts. Microb Cell Fact 2006, 5:39. 10.1186/1475-2859-5-39, 1781073, 17169150.
25. Resina D, Bollok M, Khatri NK, Valero F, Neubauer P, Ferrer P. Transcriptional response of P. pastoris in fed-batch cultivations to Rhizopus oryzae lipase production reveals UPR induction. Microb Cell Fact 2007, 6:21. 10.1186/1475-2859-6-21, 1950523, 17634115.
26. Krainer FW, Dietzsch C, Hajek T, Herwig C, Spadiut O, Glieder A. Recombinant protein expression in Pichia pastoris strains with an engineered methanol utilization pathway. Microb Cell Fact 2012, 11:22. 10.1186/1475-2859-11-22, 3295664, 22330134.
27. Cregg JM, Barringer KJ, Hessler AY, Madden KR. Pichia pastoris as a host system for transformations. Mol Cell Biol 1985, 5(12):3376-3385. 369166, 3915774.
28. Tschopp JF, Brust PF, Cregg JM, Stillman CA, Gingeras TR. Expression of the lacZ gene from two methanol-regulated promoters in Pichia pastoris. Nucleic Acids Res 1987, 15(9):3859-3876. 10.1093/nar/15.9.3859, 340787, 3108861.
29. Solórzano L. Determination of ammonia in natural waters by the phenolhypochlorite methods. Limnol Oceanogr 1969, 14:799-801.
30. Zhang X, Bremer H. Control of the Escherichia coli rrnB P1 promoter strength by ppGpp. J Biol Chem 1995, 270(19):11181-11189. 10.1074/jbc.270.19.11181, 7538113.
31. Stephanopoulos G, Aristidou AA, Nielsen J. Metabolic Engineering: Principles and Methodologies 1998, San Diego: Academic.