Overcoming the metabolic burden of protein secretion in Schizosaccharomyces pombe - A quantitative approach using 13C-based metabolic flux analysis

Metabolic Engineering

Volumn 21, Issue , 2014, Pages 34-45

  Klein, Tobias ^a   Lange, Sabrina ^a   Wilhelm, Nadine ^b   Bureik, Matthias ^b   Yang, Tae Hoon ^c   Heinzle, Elmar ^a   Schneider, Konstantin ^a  

^a SAARLAND UNIVERSITY   (Germany)

^b PomBioTech GmbH   (Germany)

^c GENOMATICA INC   (United States)

Author keywords

Macromolecular composition; Metabolic burden; Protein secretion; Schizosaccharomyces pombe; Substrate mixtures

Indexed keywords

MACROMOLECULES; MITOCHONDRIA; PROTEINS;

BIOMASS COMPOSITIONS; MACROMOLECULAR COMPOSITION; METABOLIC BURDENS; METABOLIC FLUX ANALYSIS; PENTOSE PHOSPHATE PATHWAY; PROTEIN SECRETION; QUANTITATIVE APPROACH; SCHIZOSACCHAROMYCES POMBE;

METABOLISM;

ACETIC ACID; ALPHA GLUCOSIDASE; CARBON; CARBON 13; GLUCOSE; GLYCEROL; LIPID; PYRUVIC ACID; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE;

ARTICLE; BIOMASS; CELL METABOLISM; CHEMOSTAT; CITRIC ACID CYCLE; FUNGAL STRAIN; NONHUMAN; PENTOSE PHOSPHATE CYCLE; PRIORITY JOURNAL; PROTEIN SECRETION; SCHIZOSACCHAROMYCES POMBE;

SCHIZOSACCHAROMYCES POMBE;

(13)C METABOLIC FLUX ANALYSIS; MACROMOLECULAR COMPOSITION; METABOLIC BURDEN; PROTEIN SECRETION; SCHIZOSACCHAROMYCES POMBE; SUBSTRATE MIXTURES;

ALPHA-GLUCOSIDASES; CARBON ISOTOPES; ISOTOPE LABELING; METABOLOME; RECOMBINANT PROTEINS; SCHIZOSACCHAROMYCES;

EID: 84888807683     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2013.11.001     Document Type: Article

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