Hypoxic fed-batch cultivation of Pichia pastoris increases specific and volumetric productivity of recombinant proteins

Biotechnology and Bioengineering

Volumn 100, Issue 1, 2008, Pages 177-183

  Baumann, Kristin ^a   Maurer, Michael ^b,c   Dragosits, Martin ^b   Cos, Oriol ^a   Ferrer, Pau ^a   Mattanovich, Diethard ^b,c  

^a UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

^b UNIVERSITY OF AGRICULTURAL SCIENCES   (Austria)

^c Fachhochschule Campus Wien   (Austria)

Author keywords

Antibody fragment; Oxygen limitation; Pichia pastoris; Productivity; Trypsinogen

Indexed keywords

CELL CULTURE; CHEMOSTATS; ETHANOL; OXYGEN; PROTEINS;

ANTIBODY FRAGMENT; OXYGEN LIMITATION; PICHIA PASTORIS; TRYPSINOGEN;

ENZYME KINETICS;

ALCOHOL; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; IMMUNOGLOBULIN F(AB) FRAGMENT; RECOMBINANT PROTEIN; TRYPSINOGEN;

ARTICLE; CELL DENSITY; CHEMOSTAT; CONTROLLED STUDY; FED BATCH CULTURE; FEEDBACK SYSTEM; FUNGAL BIOMASS; FUNGUS CULTURE; HYPOXIA; NONHUMAN; OXIDATION; PICHIA PASTORIS; PROTEIN SYNTHESIS; VOLUMETRY;

BIOREACTORS; CELL CULTURE TECHNIQUES; CELL HYPOXIA; CELL PROLIFERATION; CELL SURVIVAL; OXYGEN; PICHIA; PROTEIN ENGINEERING; RECOMBINANT PROTEINS;

PICHIA PASTORIS;

EID: 42549132542     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.21763     Document Type: Article

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