Collisional fragmentation of central carbon metabolites in LC-MS/MS increases precision of 13C metabolic flux analysis

Biotechnology and Bioengineering

Volumn 109, Issue 3, 2012, Pages 763-771

  Rühl, Martin ^a,b   Rupp, Beat ^a   Nöh, Katharina ^c   Wiechert, Wolfgang ^c   Sauer, Uwe ^a   Zamboni, Nicola ^a  

^a ETH ZURICH   (Switzerland)

^b Life Science Zurich   (Switzerland)

^c FORSCHUNGSZENTRUM JÜLICH   (Germany)

Author keywords

13C; Flux analysis; Isotopomer balancing; LC MS

Indexed keywords

13C; ANALYTICAL STRATEGY; BACILLUS SUBTILIS; CARBON METABOLISM; EXPERIMENTAL DETERMINATION; FLUX ANALYSIS; FLUX ESTIMATION; IN-DEPTH ANALYSIS; IN-SILICO; ISOTOPOLOGUES; LC-MS; LC-MS/MS; METABOLIC FLUX ANALYSIS; PENTOSE PHOSPHATE PATHWAY; POSITIONAL INFORMATION; POTENTIAL BENEFITS; SENSITIVE MEASUREMENT; TANDEM MS; TEMPORAL RESOLUTION; TRICARBOXYLIC ACID CYCLE;

BACTERIOLOGY; ESTIMATION; GLUCOSE; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; METABOLITES;

METABOLISM;

CARBON 13; GLUCOSE; PENTOSE PHOSPHATE; TRICARBOXYLIC ACID;

ACCURACY; ARTICLE; BACILLUS SUBTILIS; CARBON METABOLISM; CITRIC ACID CYCLE; FRAGMENTATION REACTION; GLYCOLYSIS; ISOTOPE ANALYSIS; LIQUID CHROMATOGRAPHY; METABOLITE; NONHUMAN; PENTOSE PHOSPHATE CYCLE; TANDEM MASS SPECTROMETRY;

BACILLUS SUBTILIS; CARBON; CARBON ISOTOPES; CHROMATOGRAPHY, LIQUID; CULTURE MEDIA; ISOTOPE LABELING; ORGANIC CHEMICALS; SPECIMEN HANDLING; TANDEM MASS SPECTROMETRY;

BACILLUS SUBTILIS;

EID: 84855977005     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24344     Document Type: Article

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