Central metabolic responses to the overproduction of fatty acids in Escherichia coli based on 13C-metabolic flux analysis

Biotechnology and Bioengineering

Volumn 111, Issue 3, 2014, Pages 575-585

  He, Lian ^a   Xiao, Yi ^a   Gebreselassie, Nikodimos ^b   Zhang, Fuzhong ^a   Antoniewicz, Maciek R ^b   Tang, Yinjie J ^a   Peng, Lifeng ^a,b,c  

^a WASHINGTON UNIVERSITY IN ST LOUIS   (United States)

^b UNIVERSITY OF DELAWARE   (United States)

^c VICTORIA UNIVERSITY OF WELLINGTON   (New Zealand)

Author keywords

Cofactor balance; Energy balance; Isotope labeling; Maintenance energy; Mass spectrometry; Metabolic engineering

Indexed keywords

COFACTOR BALANCES; ENTNER-DOUDOROFF PATHWAYS; EXPONENTIAL GROWTH PHASE; FATTY ACID PRODUCTIONS; ISOTOPE LABELING; PENTOSE PHOSPHATE PATHWAY; REAL-TIME QUANTITATIVE RT-PCR; SOLUBLE TRANSHYDROGENASE;

BATCH CELL CULTURE; CARBOXYLATION; ENCODING (SYMBOLS); ENERGY BALANCE; ESCHERICHIA COLI; FATTY ACIDS; GLUCOSE; ISOTOPES; MAINTENANCE; MASS SPECTROMETRY; METABOLISM; STRAIN;

METABOLIC ENGINEERING;

ACETIC ACID; ADENOSINE TRIPHOSPHATE; BACTERIAL PROTEIN; CARBON 13; FADR PROTEIN; FATTY ACID; GLUCOSE; NICOTINAMIDE ADENINE DINUCLEOTIDE (PHOSPHATE) TRANSHYDROGENASE; PENTOSE PHOSPHATE; PHOSPHOENOLPYRUVATE; PNTA PROTEIN; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; TESA PROTEIN; UDHA PROTEIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIUM CULTURE; BASAL METABOLIC RATE; BIOENGINEERING; CARBOXYLATION; CONTROLLED STUDY; ENERGY BALANCE; ENTNER DOUDOROFF PATHWAY; ESCHERICHIA COLI; FATTY ACID METABOLISM; FATTY ACID SYNTHESIS; GENE OVEREXPRESSION; HYDROGENATION; METABOLIC FLUX ANALYSIS; METABOLIC PARAMETERS; MICROBIAL BIOMASS; NONHUMAN; PENTOSE PHOSPHATE CYCLE; PROTEIN FOLDING; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION;

ESCHERICHIA COLI;

ADENOSINE TRIPHOSPHATE; AEROBIOSIS; ENERGY METABOLISM; ESCHERICHIA COLI; FATTY ACIDS; GENE DELETION; GENE EXPRESSION; GENE EXPRESSION PROFILING; GLUCOSE; METABOLIC ENGINEERING; METABOLIC FLUX ANALYSIS; METABOLIC NETWORKS AND PATHWAYS; NADP; REAL-TIME POLYMERASE CHAIN REACTION; TRANSCRIPTION, GENETIC;

EID: 84892799105     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.25124     Document Type: Article

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