Model based engineering of Pichia pastoris central metabolism enhances recombinant protein production

Metabolic Engineering

Volumn 24, Issue , 2014, Pages 129-138

  Nocon, Justyna ^a   Steiger, Matthias G ^a,d   Pfeffer, Martin ^a,e   Sohn, Seung Bum ^b   Kim, Tae Yong ^b   Maurer, Michael ^c,d   Rußmayer, Hannes ^a,d   Pflügl, Stefan ^a,d   Ask, Magnus ^a,d   Haberhauer Troyer, Christina ^a,d   Ortmayr, Karin ^a   Hann, Stephan ^a,d   Koellensperger, Gunda ^a,d   Gasser, Brigitte ^a,d   Lee, Sang Yup ^b   Mattanovich, Diethard ^a,d  

^a UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

^b Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

^c Fachhochschule Campus Wien   (Austria)

^d AUSTRIAN CENTRE OF INDUSTRIAL BIOTECHNOLOGY   (Austria)

^e ERBERAG   (Austria)

Author keywords

Flux analysis; GEM; Metabolic modeling; NADPH; Recombinant protein; Yeast

Indexed keywords

COMPUTER SIMULATION; GEMS; GENES; OXYGEN; RECOMBINANT PROTEINS; TRANSCRIPTION; YEAST;

FLUX ANALYSIS; GENOME SCALE METABOLIC MODEL; HETEROLOGOUS PROTEINS; METABOLIC MODELING; MODEL-BASED ENGINEERING; NADPH; PENTOSE PHOSPHATE PATHWAY; RECOMBINANT PROTEIN PRODUCTIONS;

METABOLISM;

BETA GLUCOSIDASE; RECOMBINANT PROTEIN; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; SUPEROXIDE DISMUTASE; COPPER ZINC SUPEROXIDE DISMUTASE; NICOTINAMIDE ADENINE DINUCLEOTIDE;

ARTICLE; BIOENGINEERING; CITRIC ACID CYCLE; CONTROLLED STUDY; CYTOSOL; DELETION MUTANT; DNA MODIFICATION; FUNGAL METABOLISM; GENE OVEREXPRESSION; GLYCOLYSIS; IN VIVO STUDY; NONHUMAN; PENTOSE PHOSPHATE CYCLE; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SECRETION; BIOLOGICAL MODEL; BIOSYNTHESIS; GENE EXPRESSION; GENETICS; HUMAN; METABOLIC ENGINEERING; METABOLISM; METABOLOME; PICHIA;

CITRIC ACID CYCLE; GENE EXPRESSION; GLYCOLYSIS; HUMANS; METABOLIC ENGINEERING; METABOLOME; MODELS, BIOLOGICAL; NAD; PICHIA; RECOMBINANT PROTEINS; SUPEROXIDE DISMUTASE;

EID: 84901621407     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2014.05.011     Document Type: Article

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