Response of fluxome and metabolome to temperature-induced recombinant protein synthesis in Escherichia coli

Journal of Biotechnology

Volumn 132, Issue 4, 2007, Pages 375-384

  Wittmann, Christoph ^a   Weber, Jan ^b   Betiku, Eriola ^b   Krömer, Jens ^a   Böhm, Daniela ^b   Rinas, Ursula ^b  

^a SAARLAND UNIVERSITY   (Germany)

^b HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

Author keywords

13C metabolic flux; Adenylate energy charge; Cellular stress; Human basic fibroblast growth factor

Indexed keywords

BIOSYNTHESIS; ESCHERICHIA COLI; FIBROBLASTS; GROWTH KINETICS;

ADENYLATE ENERGY CHARGE; CELLULAR STRESS; HUMAN BASIC FIBROBLAST GROWTH FACTORS; METABOLIC FLUX;

CELL CULTURE;

6 PHOSPHOFRUCTOKINASE; ACETIC ACID; ACETYL COENZYME A; ADENOSINE PHOSPHATE; ADENOSINE TRIPHOSPHATE; FIBROBLAST GROWTH FACTOR 2; PYRUVATE DEHYDROGENASE; PYRUVATE OXIDASE; SYNTHETASE;

ARTICLE; BACTERIAL METABOLISM; BACTERIUM CULTURE; BIOMASS PRODUCTION; CARBON METABOLISM; CITRIC ACID CYCLE; ENERGY CONSUMPTION; ESCHERICHIA COLI; GENETIC CODE; GLYCOLYSIS; GROWTH RATE; NONHUMAN; PRIORITY JOURNAL; PROTEIN SYNTHESIS; TEMPERATURE SENSITIVITY;

ADENOSINE TRIPHOSPHATE; BIOREACTORS; CARBON; CARBON ISOTOPES; CITRIC ACID CYCLE; ESCHERICHIA COLI; FIBROBLAST GROWTH FACTOR 2; HEAT; RECOMBINANT PROTEINS;

ESCHERICHIA COLI;

EID: 35748959651     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2007.07.495     Document Type: Article

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