Identification of enzymes and quantification of metabolic fluxes in the wild type and in a recombinant Aspergillus oryzae strain

Applied and Environmental Microbiology

Volumn 65, Issue 1, 1999, Pages 11-19

  Pedersen, Henrik ^a   Carlsen, Morten ^a   Nielsen, Jens ^a  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

AMYLASE; ASPARTATE AMINOTRANSFERASE; CARBOHYDRATE; CHITIN; CITRATE SYNTHASE; FUNGAL RNA; GLUCOSE 6 PHOSPHATE DEHYDROGENASE; GLUTAMATE DEHYDROGENASE; ISOCITRATE DEHYDROGENASE; MALATE DEHYDROGENASE; MANNITOL DEHYDROGENASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE;

ARTICLE; ASPERGILLUS ORYZAE; BIOMASS; CONTROLLED STUDY; CYTOSOL; ENZYME ACTIVITY; ENZYME LOCALIZATION; ENZYME SYNTHESIS; FUNGUS GROWTH; METABOLISM; MITOCHONDRION; NONHUMAN; PENTOSE PHOSPHATE CYCLE;

ASPERGILLUS ORYZAE; FUNGI;

EID: 0032931265     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.65.1.11-19.1999     Document Type: Article

References (50)

1. Armitt, S., W. McCullough, and C. F. Roberts. 1976. Analysis of acetate non-utilizing (acu) mutants in Aspergillus nidulans. J. Gen. Microbiol. 92: 263-282.
2. Barkholt, V., and A. L. Jensen. 1989. Amino acid analysis: determination of cysteine plus half-cystine in proteins after hydrochloric acid hydrolysis with a disulfide compound as additive. Anal. Biochem. 177:318-322.
3. Benthin, S., J. Nielsen, and J. Villadsen. 1991. A simple and reliable method for the determination of cellular RNA content. Biotechnol. Tech. 5:39-42.
4. Bercovitz, A., Y. Peleg, E. Battat, J. S. Rokem, and I. Goldberg. 1990. Localization of pyruvate carboxylase in organic acid-producing Aspergillus strains. Appl. Environ. Microbiol. 56:1594-1597.
5. Blumenthal, H. J. 1976. Reserve carbohydrates in fungi, p. 292-307. In J. E. Smith and D. R. Berry (ed.), The filamentous fungi, vol. II. Edward Arnold, London, United Kingdom.
6. Boehringer GmbH. Analysis kit for determination of glutamate oxaloacetate transaminase activity. Boehringer, Mannheim, Germany.
7. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
8. Brown, C. E., and A. H. Romano. 1969. Evidence against necessary phosphorylation during hexose transport in Aspergillus nidulans. J. Bacteriol. 100:1198-1203.
9. Cabib, E. 1987. The synthesis and degradation of chitin, p. 59-99. In A. Meister (ed.), Advances in enzymology. John Wiley & Sons, New York, N. Y.
10. Carlsen, M., J. Marcher, and J. Nielsen. 1994. An improved FIA-system for measuring α-amylase in cultivation media. Biotechnol. Tech. 8:479-482.
11. Carlsen, M., J. Nielsen, and J. Villadsen. 1996. Growth and α-amylase production by Aspergillus oryzae during continuous cultivations. J. Biotechnol. 45:81-93.
12. Cartwright, C. P., A. H. Rose, J. Calderbank, and M. H. J. Keenan. 1989. Solute transport, p. 5-56. In A. H. Rose and J. S. Harrison (ed.), The yeasts, vol. 3. Academic Press, London, United Kingdom.
13. 2. Can. J. Microbiol. 31:352-355.
14. Christensen, T., H. Woeldike, E. Boel, S. B. Mortensen, K. Hjortshoej, L. Thim, and M. T. Hansen. 1988. High level expression of recombinant genes in Aspergillus oryzae. Bio/Technology 6:1419-1422.
15. Cook, R. A., and B. D. Sanwal. 1969. Isocitrate dehydrogenase (NAD-specific) from Neurospora crassa. Methods Enzymol. 13:42-47.
16. Folch, J., M. Lees, and G. H. S. Stanley. 1957. A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226:497-509.
17. Harding, R. W., D. F. Caroline, and R. P. Wagner. 1970. The pyruvate dehydrogenase complex from the mitochondrial fraction of Neurospora crassa. Arch. Biochem. Biophys. 138:653-661.
18. Henriksen, C. M., L. H. Christensen, J. Nielsen, and J. Villadsen. 1996. Growth energetics and metabolic fluxes in continuous cultures of Penicillium chrysogenum. J. Biotechnol. 45:149-164.
19. Herbert, D., P. J. Phipps, and R. E. Strange. 1971. Chemical analysis of microbial cells. Methods Microbiol. 5B:209-344.
20. Hersbach, G. J. M., C. P. van den Beek, and P. W. M. Dijk. 1984. The penicillins: properties, biosynthesis and fermentation, p. 66-145. In E. J. Vandamme (ed.), Biotechnology of industrial antibiotics. Marcel Dekker, New York, N.Y.
21. Hondmann, D. H. A., and J. Visser. 1994. Carbon metabolism, p. 61-139. In S. D. Martinelli and J. R. Kinghorn (ed.), Aspergillus: 50 years on. Progress in industrial microbiology, vol. 29. Elsevier, Amsterdam, The Netherlands.
22. Horikoshi, K., S. Iida, and Y. Ikeda. 1965. Mannitol and mannitol dehydrogenase in conidia of Aspergillus oryzae. J. Bacteriol. 89:326-330.
23. Hult, K., and S. Gatenbeck. 1978. Production of NADPH in the mannitol cycle and its relation to polyketide formation in Alternaria alternata. Eur. J. Biochem, 88:607-612.
24. Hult, K., A. Veide, and S. Gatenbeck. 1980. The distribution of the NADPH regenerating mannitol cycle among fungal species. Arch. Microbiol. 128:253-255.
25. Hunter, K., and A. H. Rose. 1971. Yeast lipids and membranes, p. 211-270. In A. H. Rose and J. S. Harrison (ed.). The yeasts. Physiology and biochemistry of yeasts, vol. II. Academic Press, London, United Kingdom.
26. Jaklitsch, W. M., C. P. Kubicek, and M. C. Scrutton. 1991. Intracellular location of enzymes involved in citrate production by Aspergillus niger. Can. J. Microbiol. 37:823-827.
27. Jones, E. W., and G. R. Fink. 1982. Regulation of amino acid and nucleotide biosynthesis in yeast, p. 181-299. In J. N. Strathern, E. W. Jones, and J. R. Broach (ed.), The molecular biology of the yeast Saccharomyces. Metabolism and gene expression. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
28. Jørgensen, H., J. Nielsen, and J. Villadsen. 1995. Metabolic flux distributions in Penicillium chrysogenum during fed-batch cultivations. Biotechnol. Bioeng. 46:117-131.
29. Lara, M., L. Blanco, M. Campomanes, E. Calva, R. Palacios, and J. Mora. 1982. Physiology of ammonium assimilation in Neurospora crassa. J. Bacteriol. 150:105-112.
30. Mark, C. G., and A. H. Romano. 1971. Properties of the hexose transport systems of Aspergillus nidulans. Biochim. Biophys. Acta 249:215-226.
31. Marx, A., A. A. de Graaf, W. Wiechert, L. Eggeling, and H. Sahm. 1996. Determination of the fluxes in the central metabolism of Corynebacterium glutamicum by nuclear magnetic resonance spectroscopy combined with metabolite balancing. Biotechnol. Bioeng. 49:111-129.
32. McCorkindale, N. J. 1976. The biosynthesis of terpenes and steroids, p. 369-422. In J. E. Smith and D. R. Berry (ed.), The filamentous fungi, vol. II. Edward Arnold, London, United Kingdom.
33. McCullough, W., and A. Shanks. 1993. Properties of genes involved in the control of isocitrate lyase production in Aspergillus nidulans. J. Gen. Microbiol. 139:509-511.
34. Meixner-Monori, B., C. P. Kubicek, W. Harrer, G. Schreferl, and M. Rohr. 1986. NADP-specific isocitrate dehydrogenase from the citric acid accumulating fungus Aspergillus niger. Biochem. J. 236:549-557.
35. Nielsen, J. 1997. Physiological engineering aspects of Penicillium chrysogenum. World Scientific Publishing, Singapore.
36. Nissen, T. L., U. Schulze, J. Nielsen, and J. Villadsen. 1997. Flux distributions in anaerobic, glucose-limited continuous cultures of Saccharomyces cerevisiae. Microbiology 143:203-218.
37. Ochoa, S. 1955. Malic dehydrogenase from pig heart. Methods Enzymol. 1:735-745.
38. Osmani, S. A., and M. C. Scrutton. 1983. The sub-cellular localisation of pyruvate carboxylase and of some other enzymes in Aspergillus nidulans. Eur. J. Biochem. 133:551-560.
39. Paszewski, A., and J. Grabski. 1975. Enzymatic lesions in methionine mutants of Aspergillus nidulans: role and regulation of an alternative pathway for cysteine and methionine synthesis. J. Bacteriol. 124:893-904.
40. Pateman, J. A., and J. R. Kinghorn. 1976. Nitrogen metabolism, p. 159-237. In J. E. Smith and D. R. Berry (ed.). The filamentous fungi, vol. II. Edward Arnold, London, United Kingdom.
41. Pronk, J. T., H. Y. Steensma, and J. P. van Dijken. 1996. Pyruvate metabolism in Saccharomyces cerevisiae. Yeast 12:1607-1633.
42. Rosenberger, R. F. 1976. The cell wall, p. 328-344. In J. E. Smith and D. R. Berry (ed.), The filamentous fungi, vol. II. Edward Arnold, London, United Kingdom.
43. Sauer, U., V. Hatzimanikatis, H. P. Hohmann, M. Manneberg, A. P. G. M. van Loon, and J. E. Bailey. 1996. Physiology and metabolic fluxes of wild-type and riboflavin-producing Bacillus subtilis. Appl. Environ. Microbiol. 62:3687-3696.
44. Schwitzguebel, J. P., I. M. Möller, and J. M. Palmer. 1981. Changes in density of mitochondria and glyoxysomes from Neurospora crassa: a reevaluation using silica sol gradient centrifugation. J. Gen. Microbiol. 126: 289-295.
45. Singh, M., N. S. Scrutton, and M. C. Scrutton. 1988. NADPH generation in Aspergillus nidulans: is the mannitol cycle involved? J. Gen. Microbiol. 134: 543-654.
46. Spohr, A., M. Carlsen J. Nielsen, and J. Villadsen. 1998. α-Amylase production in recombinant Aspergillus oryzae during fed-batch and continuous cultivations. J. Ferment. Bioeng. 86:49-56.
47. Stephanopolus, G., and J. J. Vallino. 1991. Network rigidity and metabolic engineering in metabolite overproduction. Science 252:1675-1681.
48. Tuite, M. F. 1989. Protein synthesis, p. 161-204. In A. H. Rose and J. S. Harrison (ed.). The yeasts. Academic Press, London, United Kingdom.
49. Walker, P., and M. Woodbine. 1976. The biosynthesis of fatty acids, p. 137-158. In J. E. Smith and D. R. Berry (ed.), The filamentous fungi, vol. II. Edward Arnold, London, United Kingdom.
50. Wennekes, L. M. J., T. Goosen, P. J. M. van den Broek, and H. W. J. van den Broek. 1993. Purification and characterization of glucose-6-phosphate dehydrogenase from Aspergillus nidulans. J. Gen. Microbiol. 139:2793-2800.