Metabolic adaptation of Escherichia coli during temperature-induced recombinant protein production: 2. Redirection of metabolic fluxes

Biotechnology and Bioengineering

Volumn 80, Issue 3, 2002, Pages 320-330

  Weber, Jan ^a,b   Hoffmann, Frank ^a,c   Rinas, Ursula ^a  

^a HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

^b F HOFFMANN LA ROCHE LTD   (Switzerland)

^c MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

Author keywords

Escherichia coli; Metabolic burden; Metabolic flux analysis; Recombinant protein

Indexed keywords

ADENOSINETRIPHOSPHATE; ESCHERICHIA COLI; LINEAR PROGRAMMING; METABOLISM; STOICHIOMETRY;

METABOLIC FLUXES;

BIOTECHNOLOGY;

BASIC FIBROBLAST GROWTH FACTOR; HEAT SHOCK PROTEIN; NICOTINAMIDE ADENINE DINUCLEOTIDE (PHOSPHATE) TRANSHYDROGENASE; RECOMBINANT PROTEIN; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE;

ARTICLE; BACTERIAL METABOLISM; BIOSYNTHESIS; CALCULATION; CATABOLISM; CELL CULTURE; CITRIC ACID CYCLE; ENERGY; ESCHERICHIA COLI; GLUCOSE METABOLISM; PENTOSE PHOSPHATE CYCLE; PROTEIN SYNTHESIS; RESPIRATORY CHAIN; STOICHIOMETRY; SYSTEM ANALYSIS; TEMPERATURE;

ADAPTATION, BIOLOGICAL; CITRIC ACID CYCLE; COMPUTER SIMULATION; ESCHERICHIA COLI; FIBROBLAST GROWTH FACTOR 2; GENE EXPRESSION; GENE EXPRESSION REGULATION, BACTERIAL; GLUCOSE; HUMANS; MODELS, BIOLOGICAL; MODELS, CHEMICAL; NADP; OXIDOREDUCTASES; OXYGEN; PLASMIDS; PROGRAMMING, LINEAR; RECOMBINANT PROTEINS; REFERENCE VALUES; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; TEMPERATURE;

ESCHERICHIA COLI; VECTORS;

EID: 0037027392     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10380     Document Type: Article

References (39)

1. Andersen KB, von Meyenburg K. 1977. Charges of nicotinamide adenine nucleotides and adenylate energy charge as regulatory parameters of the metabolism in Escherichia coli. J Biol Chem 252:4151-4156.
2. Benthin S, Nielsen J, Villadsen J. 1991. A simple and reliable method for the determination of cellular RNA content. Biotechnol Tech 5:39-42.
3. Bonarius HPJ, Hatzimanikatis V, Meesters KPH, de Gooijer CD, Schmid G, Tramper J. 1996. Metabolic flux analysis of hybridoma cells in different culture media using mass balances. Biotechnol Bioeng 50:299-318.
4. 13C-tracer experiments to determine metabolic fluxes in hybridoma cells. Biotechnol Bioeng 58:258-262.
5. Bonsignore A, de Flora A. 1972. Regulatory properties of glucose-6-phosphate dehydrogenase. Curr Top Cell Reg 6:21-36.
6. Boonstra B, French CE, Wainwright I, Bruce NC. 1999. The udhA gene of Escherichia coli encodes a soluble pyridine nucleotide transhydrogenase. J Bacteriol 181:1030-1034.
7. Bragg PD, Davies PL, Hou C. 1972. Function of energy-dependent transhydrogenase in Escherichia coli. Biochem Biophys Res Commun 47:1248-1255.
8. Calik P, Calik G, Takac S, Ozdamar TH. 1999. Metabolic flux analysis for serine alkaline protease fermentation by Bacillus licheniformis in a defined medium: Effects of the oxygen transfer rate. Biotechnol Bioeng 64:151-167.
9. Daae EB, Ison AP. 1999. Classification and sensitivity analysis of a proposed primary metabolic reaction network for Streptomyces lividans. Metabol Eng 1:153-165.
10. Dave E, Guest JR, Attwood MM. 1995. Metabolic engineering in Escherichia coli: Lowering the lipoyl domain content of the pyruvate dehydrogenase complex adversely affects the growth rate and yield. Microbiology 141:1839-1849.
11. Dong H, Nilsson L; Kurland CG. 1995. Gratuitous overexpression of genes in Escherichia coli leads to growth inhibition and ribosome destruction. J Bacteriol 177:1497-1504.
12. George HA, Powell AL, Dahlgren ME, Herber WK, Maigetter RZ, Burgess BW, Stirdivant SM, Greasham RL. 1992. Physiological effects of TGFα-PE40 expression in recombinant Escherichia coli JM109. Biotechnol Bioeng 40:437-445.
13. Gschaedler A, Boudrant J. 1994. Amino acid utilization during batch and continuous cultures of Escherichia coli on a semi-synthetic medium. J Biotechnol 37:235-251.
14. Herbert D, Phipps PJ, Strange RE. 1971. Chemical analysis of microbial cells. In: Norris JR, Ribbons DW, editors. Methods in Microbiology. London: Academic Press, 5b. p 209-344.
15. Hoffmann F, Rinas U. 2000. Kinetics of heat-shock response and inclusion body formation during temperature-induced production of basic fibroblast growth factor in high-cell density cultures of recombinant Escherichia coli. Biotechnol Prog 16:1000-1007.
16. Hoffmann F, Rinas U. 2001. On-line estimation of the metabolic burden resulting from synthesis of plasmid-encoded and heat-shock proteins by monitoring respiratory energy generation. Biotechnol Bioeng 76:333-340.
17. Hoffman F, Weber J, Rinas U. 2002. Metabolic adaptation of Escherichia coli during temperature-induced recombinant protein production: 1. Readjustment of metabolic enzyme synthesis. Biotechnol Bioeng 80:313-319.
18. Holms WH. 1986. The central metabolic pathways of Escherichia coli: Relationship between flux and control at a branch point, efficiency of conversion to biomass, and excretion of acetate. Curr Top Cell Reg 28:69-105.
19. Ingraham JL, Maaløe O, Neidhardt FC. 1983. Growth of the bacterial cell. In: Composition, Organization, and Structure of the Bacterial Cell. Sunderland, MA: Sinauer. p 1-48.
20. Karp PD, Riley M, Paley S, Pellegrini-Toole A, Krummenacker M. 1999. EcoCyc: Encyclopedia of Escherichia coli genes and metabolism. Nucl Acids Res 27:55-58.
21. Kayser A. 1999. Stoffflüsse in Escherichia Coli TGI Unter Aeroben, Glucoselimitierten Bedingungen. Dissertation, TU Braunschweig.
22. Parsell DA, Sauer RT. 1989. Induction of a heat shock-like response by unfolded protein in Escherichia coli: Dependence on protein level not protein degradation. Genes Dev 3:1226-1232.
23. Pramanik J, Keasling JD. 1997. Stoichiometric model of Escherichia coli metabolism: Incorporation of growth-rate dependent biomass composition and mechanistic energy requirements. Biotechnol Bioeng 56:398-421.
24. Ramirez DM, Bentley WE. 1993. Enhancement of recombinant protein synthesis and stability via coordinated amino acid addition. Biotechnol Bioeng 41:557-565.
25. Rydström J. 1977. Energy-linked nicotinamide nucleotide transhydrogenases. Biochim Biophys Acta 463:155-184.
26. Schilling CH, Edwards JS, Palsson BO. 1999. Toward metabolic phenomics: Analysis of genomic data using flux balances. Biotechnol Prog 15:288-295.
27. Stouthamer AH. 1973. A theoretical study on the amount of ATP required for synthesis of microbial cell material. Antonie Van Leeuwenhoek 39:545-565.
28. Stouthamer AH, Bettenhaussen C. 1973. Utilization of energy for growth and maintenance in continuous and batch cultures of microorganisms. A reevaluation of the method for the determination of the ATP production by measuring molar growth yields. Biochim Biophys Acta 301:53-70.
29. Vallino JJ, Stephanopoulos G. 1990. Flux determination in cellular bioreaction networks: Applications to lysine fermentations. In: Sikdar SK, Bier M, Todd P, editors. Frontiers in Bioprocessing. Boca Raton, FL: CRC Press. p 205-219.
30. Vallino JJ, Stephanopoulos G. 1993. Metabolic flux distributions in Corynebacterium glutamicum during growth and lysine over-production. Biotechnol Bioeng 41:633-646.
31. Vallino JJ, Stephanopoulos G. 1994. Carbon flux distributions at the glucose-6-phosphate branch point in Corynebacterium glutamicum during lysine overproduction. Biotechnol Prog 10:327-334.
32. van Gulik WM, Heijnen JJ. 1995. A metabolic network stoichiometry analysis of microbial growth and product formation. Biotechnol Bioeng 48:681-698.
33. Varma A, Palsson BO. 1993. Metabolic capabilities of Escherichia coli II. Optimal growth patterns. J Theor Biol 165:503-522.
34. Varma A, Palsson BO. 1994. Stoichiometric flux balance models quantitatively predict growth and metabolic by-product secretion in wild-type Escherichia coli W3110. Appl Environ Microbiol 60:3724-3731.
35. Viaplana E, Villaverde A. 1996. Polylinker-encoded peptides can confer toxicity to recombinant proteins produced in Escherichia coli. Biotechnol Prog 12:723-727.
36. Voordouw G, van der Vies SM, Themmen APN. 1983. Why are two different types of pyridine nucleotide transhydrogenase found in living organisms? Eur J Biochem 131:527-533.
37. Wallace RJ, Holms WH. 1986. Maintenance coefficients and rates of turn-over of cell material in Escherichia coli ML 308 at different growth temperatures. FEMS Microbiol Lett 37:317-320.
38. Zahl KJ, Rose C, Hanson RL. 1978. Isolation and partial characterization of a mutant of Escherichia coli lacking pyridine nucleotide transhydrogenase. Arch Biochem Biophys 190:598-602.
39. Zeng AP, Ross A, Deckwer W-D. 1990. A method to estimate the efficiency of oxidative phosphorylation and biomass yield from ATP of a facultative anaerobe in continuous culture. Biotechnol Bioeng 36:965-969.