Chitosans: New vectors for gene therapy

Handbook of Carbohydrate Polymers: Development, Properties and Applications

Volumn , Issue , 2011, Pages 583-604

  Muzzarelli, Riccardo A A ^a  

^a Muzzarelli Consulting   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCOMPATIBILITY; CELLS; CHITOSAN; CYTOLOGY; DNA; GENE THERAPY; GENES; MULTILAYERS; OLIGONUCLEOTIDES; POLYETHYLENES; VECTORS;

CELL-SPECIFIC LIGANDS; GREEN FLUORESCENT PROTEIN; MODIFIED CHITOSAN; PHYSIOLOGICAL MEDIA; PLASMID VECTORS; POLYETHYLENEIMINE; TIGHT JUNCTIONS; WOUND DRESSING MATERIALS;

CHITIN;

EID: 84897197744     PISSN: None     EISSN: None     Source Type: Book    
DOI: None     Document Type: Chapter

References (115)

1. Ahmed, SR; Kelly, AB; Barbari, TA. Controlling the orientation of immobilized proteins on an affinity membrane through chelation of a histidine tag to a chitosan-Nickel(II) surface. Journal of Membrane Science, 280(1-2), 2006, 553-559.
2. Andersen, MO; Howard, KA; Paludan, SR; Besenbacher, F; Kjems, J. Delivery of siRNA from lyophilized polymeric surfaces. Biomaterials, 29(4), 2008, 506-512.
3. Austin, CP. The impact of the completed human genome sequence on the development of novel therapeutics for human disease. Annual Reviews of Medicine, 2004, 55, 1-13.
4. Baty, AM III; Eastburn, CC; Diwu, Z; Techkarnjanaruk, S; Goodman, AE; Geesey, GG. Differentiation of chitinase-active and non-chitinase-active subpopulations of a marine bacterium during chitin degradation. Applied and Environmental Microbiology, 66(8), 2000, 3566-3573.
5. Chan, P; Kurisawa, M; Chung, JE; Yang, YY. Synthesis and characterization of chitosan-gpoly( ethylene glycol)-folate as a non-viral carrier for tumor-targeted gene delivery. Biomaterials 28, 2007, 540-549.
6. Chen, J; Tian, B; Yin, X; Zhang, Y; Hu, D; Hu, Z; Liu, MZ; Pan, YF; Zhao, JF; Li, H; Hou, C; Wang, JW; Zhang, YD. Preparation, characterization and transfection efficiency of cationic PEGylated PLA nanoparticles as gene delivery systems. Journal of Biotechnology, 130 (2), 15 2007, 107-113
7. Chen, TH; Small, DA; Wu, LQ; Rubloff, GW; Ghodssi, R; Vazquez-Duhalt, R; Bentley, WE; Payne, GF. Nature-inspired creation of protein-polysaccharide conjugate and its subsequent assembly onto a patterned surface. Langmuir, 19(22), 2003, 9382-9386.
8. Colonna, C; Conti, B; Genta, I; Alpar, OH. Non-viral dried powders for respiratory gene delivery prepared by cationic and chitosan loaded liposomes. International Journal of Pharmaceutics, 364(1), 2008, 108-118.
9. Cullen, R; Marshall, S. Genetic research and genetic information: a health information professional's perspective on the benefits and risks. Health Information and Libraries Journal, 2006, 23, 275-82.
10. Dana, MD; Limon, MC; Mejias, R; Mach, RL; Benitez, T; Pintor-Toro, JA; Kubicek, CP. Regulation of chitinase 33 (chit33) gene expression in Trichoderma harzianum. Current Genetics, 38(6), 2001, 335-342.
11. Dang, JM; Leong, KW. Current status of polymeric gene delivery systems. Advances in Drug Delivery Reviews, 58, 2006, 467-486.
12. Dass, CR; Contreras, KG; Dunstan, DE; Choong, PFM. Chitosan microparticles encapsulating PEDF plasmid demonstrate efficacy in an orthotopic metastatic model of osteosarcoma. Biomaterials, 28(19), 2007, 3026-3033.
13. Davis SS. Biomedical applications of nanotechnology: implications for drug targeting and gene therapy. Trends in Biotechnology, 15, 1997, 217-224.
14. De Britto, D; Assis, OBG. A novel method for obtaining a quaternary salt of chitosan. Carbohydrate Polymers 69 (2), 2007, 305-310.
15. Di Colo, G; Zambito, Y; Zaino, C. Polymeric enhancers of mucosal epithelia permeability: Synthesis, transepithelial penetration-enhancing properties, mechanism of action, safety issues. Journal of Pharmaceutical Sciences, 97(5), 2008, 1652-1680.
16. Dorsett, Y; Tuschl, T. Applications of siRNAs in functional genomics and potential as therapeutics. Nature Reviews-Drug Discovery, 2004, 3, 318-29.
17. Eguchi, A; Akuta, T; Okuyama, H; Senda, T; Yokoi, H; Inokuchi, H; Fujita, S; El-Aneed, A. An overview of current delivery systems in cancer gene therapy. Journal of Controlled Release, 94, 2004, 1-14.
18. Elbashir, SM; Harborth, J; Lendeckel, W; Yalcin, A; Weber, K; Tuschl, T. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature, 2001, 411, 494-8.
19. Engel, PC. Enzyme cofactors. In P. C. Engel (ed.), Enzymology LabFax, Academic, California, 1996, 244-245.
20. Furuhata, M., Kawakami, H., Toma, K., Hattori, Y., Maitani, Y., 2006. Design, synthesis and gene delivery efficiency of novel oligo-arginine-linked PEG-lipids: effect of oligoarginine length. International Journal of Pharmacy, 316, 109-116.
21. Futaki, S., 2005. Membrane-permeable arginine-rich peptides and the translocation mechanisms. Advances in Drug Delivery Reviews, 57, 547-558.
22. Gao, S; Chen, J; Xu, X; Ding, Z; Yang, Y.H; Hua, Z; Zhang, J. Galactosylated low molecular weight chitosan as DNA carrier for hepatocyte-targeting, International Journal of Pharmaceutics, 255, 2003, 57-68.
23. Gao, Y; Xu, ZH; Chen, SW; Gu, WW; Chen, LL; Li, YP (2008). Arginine-chitosan/DNA self-assemble nanoparticles for gene delivery: In vitro characteristics and transfection efficiency. International Journal of Pharmaceutics, 359(1-2), 2008, 241-246.
24. Ge, ZG; Baguenard, S; Lim, LY; Wee, A; Khor, E. Hydroxyapatite-chitin materials as potential tissue engineered bone substitutes. Biomaterials, 25(6), 2004, 1049-1058.
25. Germershaus, O; Mao, SR; Sitterberg, J; Bakowsky, U; Kissel, T. Gene delivery using chitosan, trimethyl chitosan or polyethylenglycol-graft-trimethyl chitosan block copolymers: Establishment of structure-activity relationships in vitro. Journal of Controlled Release, 125(2), 2008, 145-154.
26. Hannon, GJ; Rossi, JJ. Unlocking the potential of the human genome with RNA interference. Nature, 2004, 431, 371-378.
27. Hardt, M; Laine, RA. Mutation of active site residues in the chitin-binding domain ChBD(ChiAl) from chitinase A1 of Bacillus circulans alters substrate specificity: Use of a green fluorescent protein binding assay. Archives of Biochemistry and Biophysics, 426(2), 2004, 286-297.
28. Hejazi, R; Amiji, M. Chitosan-based gastrointestinal delivery systems. Journal of Controlled Release, 89, 2003, 151-165.
29. Howard, KA; Rahbek, UL; Liu, XD; Damgaard, CK; Glud, SZ; Andersen, MO; Hovgaard, MB; Schmitz, A; Nyengaard, JR; Besenbacher, F; Kjems, J. RNA interference in vitro and in vivo using a chitosan/siRNA nanoparticle system. Molecular Therapy, 14(4), 2006, 476-484.
30. Hu, FQ; Meng, P; Dai, YQ; Du, YZ; You, J; Wei, XH; Yuan, H. PEGylated chitosan-based polymer micelle as an intracellular delivery carrier for anti-tumor targeting therapy. European Journal of Pharmaceutics and Biopharmaceutics, 70(3), 2008, 749-757.
31. Ishii, T; Okahata, Y; Sato, T. Mechanism of cell transfection with plasmid/chitosan complexes. Biochimica et Biophysica Acta-Biomembranes, 1514(1), 2001, 51-64.
32. Jiang, HL; Kwon, JT; Kim, EM; Kim, YK; Arote, R; Jere, D; Jeong, HJ; Jang, MK; Nah, JW; Xu, CX; Park, IK; Cho, MH; Cho, CS. Galactosylated poly(ethylene glycol)-chitosangraft-polyethylenimine as a gene carrier for hepatocyte-targeting. Journal of Controlled Release, 131(2), 2008, 150-157.
33. Jiang, HL; Park, IK; Kang, ML; Yoo, HS; Choi, YJ; Akaike, T; Cho, CS. Immune stimulating activity of an atrophic rhinitis vaccine associated to pegylated chitosan microspheres in vitro. Polymers for Advances Technologies, 18(3), 2007, 220-225.
34. Jin, H; Xu, CX; Kim, HW; Chung, YS; Shin, JY; Chang, SH; Park, SJ; Lee, ES; Hwang, SK; Kwon, JT; Minai-Tehrani, A; Woo, M; Noh, MS; Youn, HJ; Kim, DY; Yoon, BI; Lee, KH; Kim, TH; Cho, S; Cho, MH. Urocanic acid-modified chitosan-mediated PTEN delivery via aerosol suppressed lung tumorigenesis in K-ras(LA1) mice. Cancer Therapy, 15(5), 2008, 275-283.
35. Katas, H; Alpar, HO. Development and characterisation of chitosan nanoparticles for siRNA delivery. Journal of Controlled Release, 115, 2006, 216-225.
36. Kim, TH; Ihm, JE; Choi, YJ; Nah, JW; Cho, CS. Efficient gene delivery by urocanic acidmodified chitosan. Journal of Controlled Release, 93, 2003, 389-402.
37. Kim, TH; Kim, SI; Akaike, T; Cho, CS. Synergistic effect of poly(ethylenimine) on the transfection efficiency of galactosylated chitosan-DNA complexes. Journal of Controlled Release, 105 (2005) 354-366.
38. Kim, TH; Nah, JW; Cho, MH; Park, TG; Cho, CS. Receptor-mediated gene delivery into antigen presenting cells using mannosylated chitosan-DNA nanoparticles. Journal of Nanoscience and Nanotechnology, 6, 2006, 2796-2803.
39. Kim, TH; Park, IK; Nah, JW; Choi, YJ; Cho, CS. Galactosylated chitosan-DNA nanoparticles prepared using water-soluble chitosan as a gene carrier. Biomaterials, 25, 2004, 3783-3792.
40. Kim, D.H., Rossi, J.J., 2007. Strategies for silencing human disease using RNA interference. Nat. Rev. Genet. 8, 173-184.
41. Kim, YH; Gihm, SH; Park, CR; Lee, KY; Kim, TW; Kwon, IC; Chung, H; Jeong, SY. Structural characteristics of size-controlled self-aggregates of deoxycholic acid-modified chitosan and their application as a DNA delivery carrier. Bioconjugate Chemistry, 12, 2001, 932-938.
42. Kneuer, C; Sameti, M; Bakowsky, U; Schiestel, T; Schirra, H; Schmidt, H. A nonviral DNA delivery system based on surface modified silica nanoparticles can efficiently transfect cells in vitro. Bioconjugate Chemistry, 2000, 11, 926-932.
43. Kumar, RMNV; Muzzarelli, RAA; Muzzarelli, C; Sashiwa, H; Domb, AJ. Chitosan chemistry and pharmaceutical perspectives. Chemical Reviews, 104, 2004, 6017-6084.
44. Lee, D; Zhang, W; Shirley, SA; Kong, X; Hellermann, GR; Lockey, RF; Mohapatra, SS Thiolated chitosan/DNA nanocomplexes exhibit enhanced and sustained gene delivery. Pharmaceutical Research, 24 (1), 2007, 157-167.
45. Lee, JI; Choi, JH; Park, BC; Park, YH; Lee, MY; Park, HM; Maeng, PJ. Differential expression of the chitin synthase genes of Aspergillus nidulans, chsA, chsB and chsC, in response to developmental status and environmental factors. Fungal Genetics and Biology, 41(6), 2004, 635-646.
46. Lee, JI; Ha, KS; Yoo, HS. Quantum-dot-assisted fluorescence resonance energy transfer approach for intracellular trafficking of chitosan/DNA complex. Acta Biomaterialia 4 (4), 2008, 791-798.
47. Lewandowski, AT; Small, DA; Chen, TH; Payne, GF; Bentley, WE. Tyrosine-based "activatable pro-tag": Enzyme-catalyzed protein capture and release. Biotechnology and Bioengineering, 93(6), 2006, 1207-1215.
48. Li, XW; Lee, DKL; Chan, ASC; Alpar, HO. Sustained expression in mammalian cells with DNA complexed with chitosan nanoparticles. Biochimica et Biophysica Acta-GSE, 1630(1), 2003, 7-18.
49. Li, Z; Zhang, M. Chitosan-alginate as scaffolding material for cartilage tissue engineering. Journal of Biomedical Material Research, A-75, 2005, 485-493.
50. Liu, WG; Zhang, JR; Cao, ZQ; Xu, FY; Yao, KD. A chitosan-arginine conjugate as a novel anticoagulation biomaterial. Journal of Material Science, Materials in Medicine, 15, 2004, 1199-1203.
51. Liu, WG; Yao, KD. Chitosan and its derivatives-a promising non-viral vector for gene transfection. Journal of Controlled Release, 83 (1), 2002, 1-11
52. Liu, XD; Howard, KA; Dong, MD; Andersen, MO; Rahbek, UL; Johnsen, MG; Hansen, OC; Besenbacher, F; Kjems, J. The influence of polymeric properties on chitosan/siRNA nanoparticle formulation and gene silencing. Biomaterials, 28(6), 2007, 1280-1288.
53. Lu, B; Xu, XD; Zhang, XZ; Cheng, SX; Zhuo, RX. Low molecular weight polyethylenimine grafted N-maleated chitosan for gene delivery: properties and in vitro transfection studies. Biomacromolecules, 9(10), 2008, 2594-2600.
54. Mansouri, S; Cuie, Y; Winnik, F; Shi, Q; Lavigne, P; Benderdour, M; Beaumont, E; Fernandes, JC. Characterization of folate-chitosan-DNA nanoparticles for gene therapy. Biomaterials, 27, 2006, 2060-2065.
55. Mao, HQ; Roy, K; Troung-Le, VL; Janes, KA; Lin, KY; Wang, Y; August, JT; Leong, KW. Chitosan-DNA nanoparticles as gene carriers: synthesis, characterization and transfection efficiency. Journal of Controlled Release, 70, 2001, 399-421.
56. Martin, H; Dagkessamanskaia, A; Satchanska, G; Dallies, N; Francois, J. KNR4, a suppressor of Saccharomyces cerevisiae cwh mutants, is involved in the transcriptional control of chitin synthase genes. Microbiology-UK, 145 (Pt 1), 1999, 249-258.
57. Medina-Kauwe, LK; Xie, J; Hamm-Alvarez, S. Intracellular trafficking of nonviral vectors. Gene Therapy, 12, 2005, 1734-51.
58. Milhavet, O; Gary, DS; Mattson, MP. RNA interference in biology and medicine. Pharmacology Reviews, 55(4), 2003, 629-48.
59. Mintzer, MA; Simanek, EE. Nonviral vectors for gene delivery. Chemical Reviews, 109(2), 2009, 259-302.
60. Mounkes, LC; Zhong, W; Cipres-Palacin, G; Heath, TD; Debs, RJ. Proteoglycans mediate cationic liposome-DNA complex-based gene delivery in vitro and in vivo. Journal of Biological Chemistry, 273, 1998, 26164-26170.
61. Muzzarelli, RAA. Chitin and chitosan hydrogels. In GO Philips & A Williams (Eds.), Handbook of hydrocolloids (2nd ed.). Cambridge, UK: Woodhead Publishing Ltd. (2009a)
62. Muzzarelli, RAA. Chitin chemistry and biochemistry. In M. Paoletti & S. Musumeci (Eds.), Binomium chitin-chitinase: Emerging issues. Hauppauge, NY: Nova Science. (2009b)
63. Muzzarelli, RAA. Chitin nanostructures in living organisms. In S. N. Gupta & D. Briggs (Eds.), Chitin in the fossil record. New York: Springer. (2009c)
64. Muzzarelli, RAA. Chitin, Oxford: Pergamon Press. (1977).
65. Muzzarelli, RAA. Chitins and chitosans for the repair of wounded skin, nerve, cartilage and bone. Carbohydrate Polymers, 76 (2) 2009d.
66. Muzzarelli, RAA. Genipin-chitosan hydrogels as biomedical and pharmaceutical aids. Carbohydrate Polymers, 76 (4) 2009e.
67. Muzzarelli, RAA; Muzzarelli, C. Chitosan chemistry: relevance to the biomedical sciences. In Advances in Polymer Science, T. Heinze, ed. 186, 151-209. Springer Verlag, Berlin, 2005.
68. Muzzarelli, RAA; Muzzarelli, C. Chitosan, a dietary supplement and a food technology commodity. In Biliaderis CG and Izydorczyk MS, Eds. Functional Food Carbohydrates. Orlando USA, Francis and Taylor. (2006)
69. Muzzarelli, RAA; Orlandini, F; Boselli, E; Frega, NG; Tosi, G; Muzzarelli, C. Chitosan taurocholate capacity to bind lipids and to undergo enzymatic hydrolysis: An in vitro model. Carbohydrate Polymers, 66(3), 2006, 363-371.
70. Nakanishi, M; Eguchi, A; Akuta, T; Nagoshi, E; Fujita, S; Okabe, J; Senda, T; Hasegawa, M. Basic peptides as functional components of non-viral gene transfer vehicles. Current Protein and Peptide Science, 4, 2003, 141-150.
71. Nie, HM; Lee, LY; Tong, H; Wang, CH. PLGA/chitosan composites from a combination of spray drying and supercritical fluid foaming techniques: New carriers for DNA delivery. Journal of Controlled Release, 129(3), 2008, 207-214.
72. Nishikawa, M; Huang, L. Nonviral vectors in the new millennium: delivery barriers in gene transfer. Human Gene Therapy, 12, 2001, 861-70.
73. Nomura, H; Baladie, B; Katayama, Y; Morshead, CM; Shoichet, MS; Tator, CH. Delayed implantation of intramedullary chitosan channels containing nerve grafts promotes extensive axonal regeneration after spinal cord injury. Neurosurgery, 63(1), 2008, 127-141.
74. O'Connor, TP; Crystal, RG. Genetic medicines: treatment strategies for hereditary disorders. Nature Reviews-Genetics, 7, 2006, 261-276.
75. Opanasopit, P; Petchsangsai, M; Rojanarata, T; Ngawhirunpat, T; Sajomsang, W; Ruktanonchai, U. Methylated N-(4-N,N-dimethylaminobenzyl) chitosan as effective gene carriers: Effect of degree of substitution. Carbohydrate Polymers, 75(1), 2009, 143-149.
76. Opanasopit, P; Sajomsang, W; Ruktanonchai, U; Mayen, V; Rojanarata, T; Ngawhirunpat, T. Methylated N-(4-pyridinylmethyl) chitosan as a novel effective safe gene carrier. International Journal of Pharmaceutics, 364 (1), 2008, 127-134.
77. Pang, HT; Chen, XG; Park, HJ; Cha, DS; Kennedy, JF. Preparation and rheological properties of deoxycholate-chitosan and carboxymethyl-chitosan in aqueous systems. Carbohydrate Polymers, 69(3), 2007, 419-425.
78. Park, IK; Kim, TH; Park, YH; Shin, BA; Choi, ES; Chowdhury, EH; Akaike, T; Cho, CS. Galactosylated chitosan-graft-poly(ethylene glycol) as hepatocyte-targeting DNA carrier, Journal of Controlled Release, 76, 2001, 349-362.
79. Pasanphan, W; Buettner, GR; Chirachanchai, S (2008). Chitosan conjugated with deoxycholic acid and gallic acid: A novel biopolymer-based additive antioxidant for polyethylene. Journal of Applied Polymer Science, 109(1), 2008, 38-46.
80. Pasanphan, W; Chirachanchai, S. Conjugation of gallic acid onto chitosan: An approach for green and water-based antioxidant. Carbohydrate Polymers 72 (1), 2008, 169-177.
81. Pille, JY; Li, H; Blot, E; Bertrand, JR; Pritchard, LL; Opolon, P; Maksimenko, A; Lu, H; Vannier, JP; Soria, J; Malvy, C; Soria, C. Intravenous delivery of anti-RhoA small interfering RNA loaded in nanoparticles of chitosan in mice: safety and efficacy in xenografted aggressive breast cancer. Human Gene Therapy, 17, 2006, 1019-1026.
82. Plasterk, RH. RNA silencing: the genome's immune system. Science, 296, 2002, 1263-5.
83. Riquelme, M; Bartnicki-Garcia, S; Gonzalez-Prieto, JM; Sanchez-Leon, E; Verdin-Ramos, JA; Beltran-Aguilar, A; Freitag, M. Spitzenkorper localization and intracellular traffic of green fluorescent protein-labeled CHS-3 and CHS-6 chitin synthases in living hyphae of Neurospora crassa. Eukaryotic Cell, 6 (10), 2007, 1853-1864.
84. Rojanarata, T; Opanasopit, P; Techaarpornkul, S; Ngawhirunpat, T; Ruktanonchai, U. Chitosan-thiamine pyrophosphate as a novel carrier for siRNA delivery. Pharmaceutical Research, 25(12), 2008, 2807-2814.
85. Rojanarata, T; Petchsangsai, M; Opanasopit, P; Ngawhirunpat, T; Ruktanonchai, U; Sajomsang, W; Tantayanon, S. Methylated N-(4-N,N-dimethylaminobenzyl) chitosan for novel effective gene carriers. European Journal of Pharmaceutics and Biopharmaceutics, 70(1), 2008, 207-214.
86. Rolland A. Nuclear gene delivery: the Trojan horse approach. Expert Opinion in Drug Delivery, 3, 2006, 1-10.
87. Romoren, K; Pedersen, S; Smistad, G; Evensen, O; Thu, BJ. The influence of formulation variables on in vitro transfection efficiency and physicochemical properties of chitosanbased polyplexes. International Journal of Pharmaceutics, 261(1-2), 2003, 115-127.
88. Roy, I; Mitra, S; Maitra, A; Mozumdar, S. Calcium phosphate nanoparticles as novel nonviral vectors for targeted gene delivery. International Journal of Pharmaceutics, 250, 2003, 25-33.
89. Roy, I; Ohulchanskyy, TY; Bharali, DJ; Pudavar, HE; Mistretta, RA; Kaur, N. Optical tracking of organically modified silica nanoparticles as DNA carriers: a nonviral, nanomedicine approach for gene delivery. Proceedings of the National Academy of Science USA, 102, 2005, 279-84.
90. Roy, I; Stachowiak, MK; Bergey, EJ. Nonviral gene transfection nanoparticles: function and applications in the brain. Nanomedicine, 4, 2008, 89-97.
91. Sadeghi, AMM; Dorkoosh, FA; Avadi, MR; Weinhold, M; Bayat, A; Delie, F; Gurny, R; Larijani, B; Rafiee-Tehrani, M; Junginger, HE. Permeation enhancer effect of chitosan and chitosan derivatives: Comparison of formulations as soluble polymers and nanoparticulate systems on insulin absorption in Caco-2 cells. European Journal of Pharmaceutics and Biopharmaceutics, 70(1), 2008, 270-278.
92. Sanpui, P; Murugadoss, A; Prasad, PVD; Ghosh, SS; Chattopadhyay, A. The antibacterial properties of a novel chitosan-Ag nanoparticle composite. International Journal of Food Microbiology, 124(2), 2008, 142-146.
93. Sato, T; Ishii, T; Okahata, Y. In vitro gene delivery mediated by chitosan. Effect of pH, serum, and molecular mass of chitosan on the transfection efficiency Biomaterials, 22(15), 2001, 2075-2080
94. Strand, SP; Issa, MM; Christensen, BE; Varum, KM; Artursson, P. Tailoring of Chitosans for Gene Delivery: Novel Self-Branched Glycosylated Chitosan Oligomers with Improved Functional Properties. Biomacromolecules, 9(11), 2008, 3268-3276.
95. Tan, WB; Jiang, S; Zhang Y. Quantum-dot based nanoparticles for targeted silencing of HER2/neu gene via RNA interference. Biomaterials, 28, 2007, 1565-1571.
96. Thanou, M; Florea, BI; Geldof, M; Junginger, HE; Borchard, G. Quaternized chitosan oligomers as novel gene delivery vectors in epithelial cell lines. Biomaterials, 23, 2002, 153-159.
97. Tong, JC; Yao, SL. Novel scaffold containing transforming growth factor-beta1 DNA for cartilage tissue engineering. Journal of Bioactive and Compatible Polymers, 22(2), 2007, 232-244.
98. Tung, CH; Weissleder, R. Arginine containing peptides as delivery vectors. Advances in Drug Delivery Reviews, 55, 2003, 281-294.
99. Verma, IM; Somia, N. Gene therapy: promises, problems and prospects. Nature, 389, 1997, 239-242.
100. Vogelstein, B; Kinzler, KW. Cancer genes and the pathways they control. Nature Medicine, 10, 2004, 789-799.
101. Wan, A; Sun, Y; Li, HL. Characterization of folate-graft-chitosan as a scaffold for nitric oxide release. International Journal of Biological Macromolecules, 43(5), 2008, 415-421.
102. Wang, W; Yao, J; Zhou, JP; Lu, Y; Wang, Y; Tao, L; Li, YP. Urocanic acid-modified chitosan-mediated p53 gene delivery inducing apoptosis of human hepatocellular carcinoma cell line HepG(2) is involved in its antitumor effect in vitro and in vivo. Biochemical and Biophysical Research Communications, 377(2), 2008, 567-572.
103. Weecharangsan, W; Opanasopit, P; Ngawhirunpat, T; Apirakaramwong, A; Rojanarata, T; Ruktanonchai, U; Lee, RJ. Evaluation of chitosan salts as non-viral gene vectors in CHOK1 cells. International Journal of Pharmaceutics, 348(1-2), 2008, 161-168.
104. Weecharangsan, W; Opanasopit, P; Ngawhirunpat, T; Rojanarata, T; Apirakaramwong, A. Chitosan lactate as a nonviral gene delivery vector in COS-1 cells. AAPS Pharmscitech, 7(3), 2006, 254-259.
105. Wu, YD; Liu, CB; Zhao, XY; Xiang, JN. A new biodegradable polymer: PEGylated chitosang-PEI possessing a hydroxyl group at the PEG end. Journal of Polymer Research, 15(3), 2008, 181-185.
106. Xu, XM; Capito, RM; Spector, M. Plasmid size influences chitosan nanoparticle mediated gene transfer to chondrocytes Journal of Biomedical Materials Research Part A, 84A (4), 2008, 1038-1048.
107. Yang, Q; Li, SA; Pan, XJ. Synthesis of fluorescent chitosan and its application in noncovalent functionalization of carbon nanotubes. Biomacromolecules, 9(12), 2008, 3422-3426.
108. Yao, Z; Zhang, C; Ping, QN; Yu, LLL. A series of novel chitosan derivatives: Synthesis, characterization and micellar solubilization of paclitaxel. Carbohydrate Polymers, 68(4), 2007, 781-792.
109. Zambito, Y; Zaino, C; Burchielli, S; Carelli, V; Serafini, MF; Di Colo, G. Novel quaternary ammonium chitosan derivatives for the promotion of intraocular drug absorption. Journal of Drug Delivery Science and Technology, 17(1), 2007, 19-24.
110. Zeilinger, S; Galhaup, C; Payer, K; Woo, SL; Mach, RL; Fekete, C; Lorito, M; Kubicek, CP. Chitinase gene expression during mycoparasitic interaction of Trichoderma harzianum with its host. Fungal Genetics and Biology, 26(2), 1999, 131-140.
111. Zhang, H; Mardyani, S; Chan, WC; Kumacheva, E. Design of biocompatible chitosan microgels for targeted pH-mediated intracellular release of cancer therapeutics. Biomacromolecules, 7, 2006, 1568-1572.
112. Zhang, YF; Cheng, XR; Chen, Y; Shi, B; Chen, XH; Xu, DX; Ke, J. Three-dimensional nanohydroxyapatite/chitosan scaffolds as potential tissue engineered periodontal tissue. Journal of Biomaterials Applications, 21(4), 2007, 333-349.
113. Zhang, YQ; Chen, JJ; Zhang, YD; Pan, YF; Zhao, JF; Ren, LF; Liao, MM; Hu, ZY; Kong, L; Wang, JW. A novel PEGylation of chitosan nanoparticles for gene delivery. Biotechnology and Applied Biochemistry, 46(Pt 3-4), 2007, 197-204.
114. Zhao, X: Yu, SB; Wu, FL; Mao, ZB; Yu, CL. Transfection of primary chondrocytes using chitosan-pEGFP nanoparticles. Journal of Controlled Release, 112, 2006, 223-228.
115. Zheng, F; Shi, XW; Yang, GF; Gong, LL; Yuan, HY; Cui, YJ; Wang, Y; Du, YM; Li, Y. Chitosan nanoparticle as gene therapy vector via gastrointestinal mucosa administration: Results of an in vitro and in vivo study. Life Sciences, 80(4), 2007, 388-396.