|
|
Volumn 426, Issue 2, 2004, Pages 286-297
|
|
Mutation of active site residues in the chitin-binding domain ChBD ChiA1 from chitinase A1 of Bacillus circulans alters substrate specificity: Use of a green fluorescent protein binding assay
|
|
Author keywords
Bacillus circulans; Chitin binding domain; Green fluorescent protein; Ligand binding; Site directed mutagenesis; Substrate specificity
|
|
Indexed keywords
CHITIN DERIVATIVE;
CHITINASE;
CHITOSAN;
CURDLAN;
GREEN FLUORESCENT PROTEIN;
HYBRID PROTEIN;
POLYHISTIDINE;
TRYPTOPHAN;
AMINO ACID SUBSTITUTION;
AMINO TERMINAL SEQUENCE;
ANALYTIC METHOD;
ARTICLE;
BACILLUS CIRCULANS;
BINDING AFFINITY;
BINDING SITE;
ENZYME ACTIVE SITE;
ENZYME SPECIFICITY;
ENZYME SUBSTRATE;
ESCHERICHIA COLI;
EXPRESSION VECTOR;
GENE FUSION;
GENE MUTATION;
IMMUNOELECTROPHORESIS;
ISOTHERM;
MATHEMATICAL MODEL;
MUTAGENESIS;
NONHUMAN;
NONLINEAR SYSTEM;
PRIORITY JOURNAL;
PROTEIN BINDING;
PROTEIN DEPLETION;
PROTEIN DOMAIN;
PROTEIN EXPRESSION;
WILD TYPE;
AMINO ACID SEQUENCE;
AMINO ACID SUBSTITUTION;
BACILLUS;
BINDING SITES;
CHITIN;
CHITINASE;
COMPUTER SIMULATION;
ENZYME ACTIVATION;
GREEN FLUORESCENT PROTEINS;
LUMINESCENT PROTEINS;
MICROSCOPY, FLUORESCENCE;
MODELS, MOLECULAR;
MOLECULAR SEQUENCE DATA;
MUTAGENESIS, SITE-DIRECTED;
MUTATION;
PROTEIN BINDING;
PROTEIN STRUCTURE, TERTIARY;
RECOMBINANT FUSION PROTEINS;
SEQUENCE HOMOLOGY, AMINO ACID;
SPECTROMETRY, FLUORESCENCE;
STRUCTURE-ACTIVITY RELATIONSHIP;
SUBSTRATE SPECIFICITY;
TISSUE DISTRIBUTION;
BACILLUS CIRCULANS;
ESCHERICHIA COLI;
FUNGI;
|
|
EID: 2542475295
PISSN: 00039861
EISSN: None
Source Type: Journal
DOI: 10.1016/j.abb.2004.03.017 Document Type: Article |
|
Times cited : (66)
|
|
References (60)
|
