Tyrosine-based "activatable pro-tag": Enzyme-catalyzed protein capture and release

Biotechnology and Bioengineering

Volumn 93, Issue 6, 2006, Pages 1207-1215

  Lewandowski, Angela T ^a,b   Small, David A ^a,b   Chen, Tianhong ^a,c,d   Payne, Gregory F ^a,c   Bentley, William E ^a,b  

^a UNIVERSITY OF MARYLAND BIOTECHNOLOGY INSTITUTE   (United States)

^b UNIVERSITY OF MARYLAND   (United States)

^c University of Marylandw Baltimore County   (United States)

^d TA INSTRUMENTS   (United States)

Author keywords

Capture; Chitosan conjugation; Pro tag; Protein purification; Release; Tyrosinase; Tyrosine tag

Indexed keywords

CATALYSIS; CHROMATOGRAPHY; ENZYMES; PH EFFECTS; POLYSACCHARIDES; PURIFICATION;

CAPTURE; CHITOSAN CONJUGATION; PRO-TAG; PROTEIN PURIFICATION; RELEASE; TYROSINASE; TYROSINE TAG;

PROTEINS;

CELL EXTRACT; CHITOSAN; ENTEROPEPTIDASE; GREEN FLUORESCENT PROTEIN; TYROSINE;

ARTICLE; ENZYME ACTIVATION; NONHUMAN; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ENGINEERING; PROTEIN PURIFICATION; PROTEIN SECRETION; WESTERN BLOTTING;

BIOTECHNOLOGY; CHITOSAN; CHROMATOGRAPHY, AFFINITY; ENTEROPEPTIDASE; ENZYMES; ESCHERICHIA COLI; GLYCOSIDE HYDROLASES; GREEN FLUORESCENT PROTEINS; HISTIDINE; MONOPHENOL MONOOXYGENASE; NICKEL; OLIGOPEPTIDES; PEPTIDES; PRECIPITATION; RECOMBINANT FUSION PROTEINS;

EID: 33646030628     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.20840     Document Type: Article

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