Chitosan-DNA nanoparticles as gene carriers: Synthesis, characterization and transfection efficiency

Journal of Controlled Release

Volumn 70, Issue 3, 2001, Pages 399-421

  Mao, Hai Quan ^a   Roy, Krishnendu ^a   Troung Le, Vu L ^a   Janes, Kevin A ^a   Lin, Kevin Y ^a   Wang, Yan ^a   August, J Thomas ^a   Leong, Kam W ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Chitosan; Complex coacervation; Gene delivery; Nanoparticles; Receptor mediated

Indexed keywords

BUFFER; CHITOSAN; CHLOROQUINE; KNOB PROTEIN; LIPOFECTAMINE; MACROGOL; NANOPARTICLE; NUCLEASE; PLASMID DNA; PROTOSAN; SODIUM CHLORIDE; SODIUM SULFATE; TRANSFERRIN; UNCLASSIFIED DRUG;

ARTICLE; CELL TYPE; COACERVATION; CONTROLLED STUDY; DRUG ACTIVITY; ELECTROPHORETIC MOBILITY; EMBRYO; ENCAPSULATION; ENZYME DEGRADATION; FIBROBLAST CULTURE; FREEZE DRYING; GENE EXPRESSION; GENETIC TRANSFECTION; HELA CELL; HUMAN; HUMAN CELL; MOLECULAR WEIGHT; PH; PRIORITY JOURNAL; SURFACE CHARGE; SYNTHESIS; TEMPERATURE; ZETA POTENTIAL;

ANIMALS; CELL LINE; CHITIN; CHITOSAN; CHLOROQUINE; DNA; GENE THERAPY; HUMANS; MICE; MICE, INBRED AKR; POLYETHYLENE GLYCOLS; TISSUE DISTRIBUTION; TRANSFECTION;

EID: 0035936972     PISSN: 01683659     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-3659(00)00361-8     Document Type: Article

References (48)

1. The gene as the drug
2. Key issues in non-viral gene delivery
3. Gene-delivery systems using cationic polymers
4. Lactose-poly(ethylene glycol)-grafted poly-L-lysine as hepatoma cell-targeted gene carrier
5. A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: Polyethylenimine
6. Polyamidoamine cascade polymers mediate efficient transfection of cells in culture
7. Biodegradable polyester, poly[alpha-(4-aminobutyl)-L-glycolic acid], as a non-toxic gene carrier
8. Oral gene delivery with chitosan-DNA nanoparticles generates immunologic protection in a murine model of peanut allergy
9. DNA-polycation nanospheres as non-viral gene delivery vehicles
10. Chitosan and depolymerized chitosan oligomers as condensing carriers for in vivo plasmid delivery
11. DNA-chitosan nanospheres: Derivatization and storage stability
12. DNA-chitosan nanospheres: Transfection efficiency and cellular uptake
13. DNA-chitosan nanospheres for gene delivery
14. Potential of low molecular mass chitosan as a DNA delivery system: Biocompatibility, body distribution and ability to complex and protect DNA
15. Biodegradation and distribution of water-soluble chitosan in mice
16. Use of chitosan as a biomaterial: Studies on its safety and hemostatic potential
17. Chitosan as a nasal delivery system: The effect of chitosan solutions on in vitro and in vivo mucociliary transport rates in human turbinates and volunteers
18. In vitro and in vivo degradation of films of chitin and its deacetylated derivatives
19. Pharmaceutical applications of chitosan
20. Trimethylated chitosan as safe transfection agent
21. Preparation of chitosan self-aggregates as a gene delivery system
22. Chitosan-based vector/DNA complexes for gene delivery: Biophysical characteristics and transfection ability
23. Controlled gene delivery by DNA-gelatin nanospheres
24. Gene transfer by DNA-gelatin nanospheres
25. In vitro evaluation of the mucoadhesive properties of chitosan microspheres
26. Quantitative determination of chitosan and the percentage of free amino groups
27. The influence of polymer structure on the interactions of cationic polymers with DNA and morphology of the resulting complexes
28. Characterization of the knob domain of the adenovirus type 5 fiber protein expressed in Escherichia coli
29. Calcium phosphate-mediated dna transfection
30. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
31. Molecular structures and properties of partially N-acetylated chitosans
32. Ion effects on ligand-nucleic acid interactions
33. Studies on chitosan 4. Lysozymic hydrolysis of partially N-acetylated chitosans
34. Properties and action pattern of a chitosanase from Bacillus sp. PI-7S
35. Transfection of folate-polylysine DNA complexes: Evidence for lysosomal delivery
36. Interaction of chloroquine with linear and supercoiled DNAs. Effect on the torsional dynamics, rigidity, and twist energy parameter
37. Receptor-mediated endocytosis: The intracellular journey of transferrin and its receptor
38. Receptor-mediated peptide delivery in pulmonary epithelial monolayers
39. Gene delivery mediated by cationic liposomes: From biophysical aspects to enhancement of transfection
40. Transferrin-polycation conjugates as carriers for DNA uptake into cells
41. Receptor ligand-facilitated gene transfer: Enhancement of liposome-mediated gene transfer and expression by transferrin
42. Peroxidase-labelling of human serum transferrin by conjugation to oligosaccharide moieties
43. Stabilized plasmid-lipid particles: Construction and characterization
44. PEGylated DNA/transferrin-PEI complexes: Reduced interaction with blood components, extended circulation in blood and potential for systemic gene delivery
45. Evidence for the role of proteoglycans in cation-mediated gene transfer
46. Non-viral gene therapy: The promise of genes as pharmaceutical products