Mechanism of cell transfection with plasmid/chitosan complexes

Biochimica et Biophysica Acta - Biomembranes

Volumn 1514, Issue 1, 2001, Pages 51-64

  Ishii, Tsuyoshi ^a   Okahata, Yoshio ^a   Sato, Toshinori ^b  

^a TOKYO INSTITUTE OF TECHNOLOGY   (Japan)

^b KEIO UNIVERSITY   (Japan)

Author keywords

Chitosan; DNA complex; Plasmid; Subcellular distribution; Transfection

Indexed keywords

CHITOSAN; DNA; FLUORESCEIN ISOTHIOCYANATE; NITROGEN; PHOSPHATE;

ARTICLE; BLOOD LEVEL; CELL ORGANELLE; CELL PH; CELL SURFACE; CELL SWELLING; CELL TRANSPORT; CONCENTRATION RESPONSE; CONFOCAL LASER MICROSCOPY; CULTURE MEDIUM; ENZYME MECHANISM; GENETIC TRANSFECTION; MOLECULAR WEIGHT; PLASMID; PRIORITY JOURNAL; PROTEIN EXPRESSION; STOICHIOMETRY;

BLOOD PROTEINS; CELL LINE; CHITIN; CHITOSAN; DOWN-REGULATION; FLUORESCEIN-5-ISOTHIOCYANATE; FLUORESCENT DYES; HYDROGEN-ION CONCENTRATION; MICROSCOPY, CONFOCAL; MOLECULAR WEIGHT; NITROGEN; PARTICLE SIZE; PHOSPHATES; PLASMIDS; PROTONS; SURFACE-ACTIVE AGENTS; TRANSFECTION; XANTHENES;

EID: 0035801903     PISSN: 00052736     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0005-2736(01)00362-5     Document Type: Article

References (41)

1. Methods of gene delivery
2. Gene therapy promises, problems and prospects
3. Cationic lipid-based gene delivery systems: Pharmaceutical perspectives
4. Interaction of polymeric and liposomal gene delivery systems with extracelluar glycosaminoglycans: Physicochemical and transfection studies
5. Mechanism of DNA release from cationic liposome/DNA complexes used in cell transfection
6. Polyethylene glycol enhances lipoplex-cell association and lipofection
7. Lipoplex-mediated gene delivery to the lung occurs within 60 minutes of intravenous administration
8. Characterization, cell uptake, and subcellular distribution of DNA complexes with lipoglutamides having tetraethylene glycol tails
9. Preparation and characterization of DNA-lipogutamate complexes
10. Analysis for the subcellular distribution of oligonucleotide/lipid complexes
11. Polyethylene glycol-grafted poly-L-lysine as polymeric gene carrier
12. Efficient gene transfer into mammalian primary endocrine cells with lipopolyamine-coated DNA
13. Potential of low molecular mass chitosan as a DNA delivery system: Biocompatibility, body distribution and ability to complex and protect DNA
14. Chitosan-based vector/DNA complexes for gene delivery: Biophysical characteristics and transfection ability
15. Polynucleotide-chitosan complex, an insoluble but reactive form of polynucleotide
16. Preparation of chitosan self-aggregates as a gene delivery system
17. DNA-polycation nanospheres as non-viral gene delivery vehicles
18. Chitosan and depolymerized chitosan oligomers as condensing carriers for in vivo plasmid delivery
19. Possibility of application of quaternary chitosan having pendant galactose residues as gene delivery tool
20. Oral gene delivery with chitosan-DNA nanoparticles generates immunologic protection in a murine model of peanut allergy
21. Formation of a DNA/polygalactosamine complex and its interaction with cells
22. In vitro gene delivery mediated by chitosan
23. Chitosan-mediated transfection of HeLa cells
24. Chitosan-membrane interactions and their probable role in chitosan-mediated transfection
25. In vitro delivery mediated by chitosan. Effect of pH, serum, and molecular weight of chitosan on transfection efficiency
26. Size reduction of galactosylated PEI/DNA complexes improves lectin-mediated gene transfer into hepatocytes
27. A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: Polyethylenimine
28. Polyethylenimine shows properties of interest for cystic fibrosis gene therapy
29. Polyethylenimine but not cationic lipids promotes transgene delivery to the nucleus in mammalian cells
30. In vitro gene delivery to hepatocytes with galactosylated polyethylenimine
31. Facial preparation of a fluorescence-labeled plasmid
32. Mechanism of cellular uptake of modified oligodeoxynucleotides containing methylphosphonate linkages
33. Structure and pharmacology of the proton-ATPases
34. Weak bases and ionophores rapidly and reversibly raise the pH of endocytic vesicles in cultured mouse fibroblasts
35. The influences of the buffer capacity of various substances on pH changes in dental plaque
36. Design of microencapsulated chitosan microspheres for colonic drug delivery
37. Diffusion and concentration profiles of drugs in gels
38. Chitosan-EDTA conjugate: A novel polymer for topical gels
39. Properties and swelling characteristics of cross-linked poly (vinyl alcohol)/chitosan blend membrane
40. Investigation of pH-sensitive drug delivery system of chitosan/gelation hybrid polymer network
41. Localization of fungal components in the pea-fusarium interaction detected immunochemically with anti-chitosan and anti-fungal cell wall antisera