In vitro gene delivery mediated by chitosan. Effect of pH, serum, and molecular mass of chitosan on the transfection efficiency

Biomaterials

Volumn 22, Issue 15, 2001, Pages 2075-2080

  Sato, Toshinori ^a,b   Ishii, Tsuyoshi ^a   Okahata, Yoshio ^a  

^a TOKYO INSTITUTE OF TECHNOLOGY   (Japan)

^b KEIO UNIVERSITY   (Japan)

Author keywords

Chitosan; Gene transfer; Lipofectin; Luciferase plasmid; Polygalactosamine; Transfection

Indexed keywords

ENZYMES; PH EFFECTS; POLYSACCHARIDES; STOICHIOMETRY; TUMORS;

GENE DELIVERY;

GENES;

AMINOPOLYSACCHARIDE; CARBOHYDRATE DERIVATIVE; CATION; CHITOSAN; LIPOFECTIN; LIPOSOME; LUCIFERASE; PLASMID DNA; POLYGALACTOSAMINE; POLYMER; RECOMBINANT DNA; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CARCINOMA CELL; CELL PH; COMPLEX FORMATION; CONTROLLED STUDY; CULTURE MEDIUM; DNA TRANSFECTION; ENZYME ACTIVITY; GENE EXPRESSION; GENE TARGETING; HUMAN; HUMAN CELL; MOLECULAR WEIGHT; MOUSE; NONHUMAN; PRIORITY JOURNAL; SERUM; STOICHIOMETRY;

ANIMALS; CHITIN; CHITOSAN; GALACTOSAMINE; GENE TRANSFER TECHNIQUES; GENETIC VECTORS; HELA CELLS; HUMANS; HYDROGEN-ION CONCENTRATION; LUCIFERASES; MICE; PLASMA; PLASMIDS; POLYSACCHARIDES; PROTEIN BINDING; TRANSFECTION; TUMOR CELLS, CULTURED;

EID: 0035424424     PISSN: 01429612     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0142-9612(00)00385-9     Document Type: Article

References (25)

1. Virosome: Cationic liposomes enhance retroviral transduction
2. PH-sensitive, cationic liposomes: A new synthetic virus-like vector
3. Factors influencing the efficiency of cationic liposome-mediated intravenous gene delivery
4. Improved DNA: Liposome complexes for increased systemic delivery and gene expression
5. Characterization, cell uptake, and subcellular distribution of DNA complexes with lipoglutamides having tetraethylene glycol tails
6. Preparation and characterization of DNA-lipoglutamate complexes
7. Organization and recognition function of gangliosides in lipid monolayer
8. In vitro and in vivo gene delivery mediated by a synthetic polycationic amino polymer
9. Degradation of partially N-acetylated chitosans with hen egg white and human lysozyme
10. Chitosan as nasal delivery system: Evolution of insulin absorption enhancement and effect on nasal membrane integrity using rat model
11. Interaction between lisomes and chitosan II: Effect of selected parameters on aggregation and leakage
12. Interaction of indomethacin with low molecular weight chitosan, and improvements of some pharmaceutical properties of indomethacin by low molecular weight of chitosan
13. Formation of a DNA/polygalactosamine complex and its interaction with cells
14. Chitosan-mediated transfection of Hela Cells
15. Chitosan-based vector/DNA complexes for gene delivery: Biophysical characteristics and transfection ability
16. Chitosan and depolymerized chitosan oligomers as condensing carriers for in vivo plasmid delivery
17. Oral gene delivery with chitosan-DNA nanoparticles generates immunologic protection in a murine model of penut allergy
18. pH response of human dental plaque to chewing gum supplemented with low molecular chitosan
19. Rheological properties of aqueous suspension of chitin crystallites
20. Fusion of cationic liposomes with mammalian cells occurs after endocytosis
21. Mechanism of DNA release from cationic liposome/DNA complexes used in cell transfection
22. Evaluation of the biological properties of soluble chitosan and chitosan microsphere
23. Potential of low molecular mass chitosan as a DNA delivery system: Biocompatibility, body distribution and ability to complex and protect DNA
24. Chitin and Chitosan activate complement via the alternative pathway
25. Cellular pH gradient in tumor versus normal tissue: Potential exploitation for the treatment of cancer