Tuning different expression parameters to achieve soluble recombinant proteins in E. coli: Advantages of high-throughput screening

Biotechnology Journal

Volumn 6, Issue 6, 2011, Pages 715-730

  Correa, Agustín ^a   Oppezzo, Pablo ^a  

^a INSTITUT PASTEUR DE MONTEVIDEO   (Uruguay)

Author keywords

Directed evolution; High throughout screening; Protein folding; Recombinant protein; Soluble protein expression

Indexed keywords

DIRECTED EVOLUTION; E. COLI; HIGH-THROUGHOUT SCREENING; HIGH-THROUGHPUT SCREENING; HIGH-THROUGHPUT SCREENING SYSTEMS; PROMOTER STRENGTH; PROTEIN SOLUBILITY; RECOMBINANT PROTEIN; SOLUBLE PROTEINS; SPECIAL CULTURE; STABLE STATE; TARGET PROTEINS;

ESCHERICHIA COLI; PROTEIN FOLDING; SOLUBILITY;

PROTEINS;

RECOMBINANT PROTEIN;

BACTERIAL STRAIN; CODON USAGE; DISULFIDE BOND; ESCHERICHIA COLI; GENE EXPRESSION; HIGH THROUGHPUT SCREENING; MOLECULAR CLONING; NONHUMAN; PARAMETER; PLASMID; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN FOLDING; REVIEW; SOLUBILITY;

CLONING, MOLECULAR; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC VECTORS; HIGH-THROUGHPUT SCREENING ASSAYS; PROTEIN FOLDING; RECOMBINANT FUSION PROTEINS; SOLUBILITY;

ENTEROBACTERIACEAE; ESCHERICHIA COLI;

EID: 79957875229     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201100025     Document Type: Review

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