A pair of ligation-independent escherichia coli expression vectors for rapid addition of a polyhistidine affinity tag to the N- or C-termini of recombinant proteins

Journal of Biomolecular Techniques

Volumn 20, Issue 5, 2009, Pages 241-248

  Dan, Hanhong ^a   Balachandran, Aru ^a   Lin, Min ^a  

^a CANADIAN FOOD INSPECTION AGENCY   (Canada)

Author keywords

Cloning; His tagged fusion protein; Protein expression

Indexed keywords

BACTERIAL PROTEIN; GLYCINE DERIVATIVE; HISTIDINE DERIVATIVE; METHIONYLHISTIDYLHISTIDYLHISTIDYLHISTIDYLHISTIDYLHISTIDYLGLYCINE; POLYHISTIDINE TAG; PRION PROTEIN; RECOMBINANT PROTEIN; THREONYLHISTIDYLHISTIDYLHISTIDYLHISTIDYLHISTIDYLHISTIDINE; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; BASE PAIRING; CARBOXY TERMINAL SEQUENCE; CLONING VECTOR; CONTROLLED STUDY; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE EXPRESSION; GENE TARGETING; LIGATION INDEPENDENT CLONING; MAMMAL; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; PROTEIN PURIFICATION;

ANIMALS; BACTERIAL PROTEINS; BASE SEQUENCE; CLONING, MOLECULAR; CODON; DNA LIGASES; DNA PRIMERS; ESCHERICHIA COLI; GENETIC VECTORS; HISTIDINE; HUMANS; MOLECULAR SEQUENCE DATA; PRIONS; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; MAMMALIAN PRION;

EID: 77953655218     PISSN: 15240215     EISSN: 19434731     Source Type: Journal    
DOI: None     Document Type: Article

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