Facile, reagentless and in situ release of Escherichia coli intracellular enzymes by heat-inducible autolytic vector for high-throughput screening

Protein Engineering, Design and Selection

Volumn 21, Issue 11, 2008, Pages 681-687

  Cai, Zhen ^a   Xu, Wanghui ^a   Xue, Rui ^a   Lin, Zhanglin ^a  

^a TSINGHUA UNIVERSITY   (China)

Author keywords

Cell lysis; E.coli; Heat inducible autolytic vector; High throughput screening

Indexed keywords

APPLICATIONS; CATALYSTS; CELL MEMBRANES; CELLS; CYTOLOGY; DIAGNOSIS; ENZYMES; ESCHERICHIA COLI; GROWTH KINETICS; NITROCELLULOSE; POLYSACCHARIDES; PROTEINS; THROUGHPUT; VECTORS; WATER POLLUTION;

AFTER HEATS; AGAR PLATES; AMADORIASE; BIOANALYTICAL; CELL LYSIS; CLINICAL DIAGNOSES; CONSISTENT; DIRECTED EVOLUTIONS; E.COLI; EFFICIENT; HIGH-THROUGHPUT SCREENING; HIGH-THROUGHPUT SCREENINGS; IN SITU CELLS; IN-SITU; INDUCTION TEMPERATURES; INDUCTION TIMES; INTRACELLULAR ENZYMES; NITROCELLULOSE MEMBRANES; REAGENTLESS; RECOMBINANT ENZYMES; WELL PLATES;

CELL GROWTH;

AGAR; CELL ENZYME; ISOPROPYL THIOGALACTOSIDE; PYROXYLIN;

ARTICLE; CELL GROWTH; CYTOLYSIS; ELECTRONICS; ENZYME INDUCTION; ESCHERICHIA COLI; EVOLUTION; GENE CASSETTE; HIGH THROUGHPUT SCREENING; MICROARRAY ANALYSIS; NONHUMAN; PRIORITY JOURNAL; TEMPERATURE; THERMOSTABILITY;

AMINO ACID OXIDOREDUCTASES; ANIMALS; ASPERGILLUS FUMIGATUS; BACTERIOLYSIS; CELLS, CULTURED; DIRECTED MOLECULAR EVOLUTION; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENETIC TECHNIQUES; GENETIC VECTORS; HOT TEMPERATURE; ISOPROPYL THIOGALACTOSIDE; TEMPERATURE; TIME FACTORS;

ESCHERICHIA COLI; PISUM SATIVUM;

EID: 54249140045     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzn049     Document Type: Article

References (35)

1. Aharoni,A., Griffiths,A.D. and Tawfik,D.S. (2005) Curr. Opin. Chem. Biol., 9, 210-216.
2. Asenjo,J.A. and Andrews,B.A. (1990). In Asenjo,J.A. (ed.), Separation Processes in Biotechnology. CRC Press, New York, pp. 177-208.
3. Chien,L.J. and Lee,C.K. (2006) Biochem. Eng. J., 28, 17-22.
4. Cull,M. and McHenry,C. (1990) Methods Enzymol., 182, 147-153.
5. Dartois,V., Baulard,A., Schanck,K. and Colson,C. (1992) Biochim. Biophys. Acta, 1131, 253-260.
6. Dhawan,M.D., Wise,F. and Baeumner,A.J. (2002) Anal. Bioanal. Chem., 374, 421-426.
7. Di Carlo,D., Jeong,K.H. and Lee,L.P. (2003) Lab. Chip, 3, 287-291.
8. Foster,D. (1992) Biotechnology, 10, 1539-1541.
9. Glieder,A., Farinas,E.T. and Arnold,F.H. (2002) Nat. Biotechnol., 20, 1135-1139.
10. Han,F.T., Wang,Y., Sims,C.E., Bachman,M., Chang,R.S., Li,G.P. and Allbritton,N.L. (2003) Anal. Chem., 75, 3688-3696.
11. Jechlinger,W., Szostak,M.P., Witte,A. and Lubitz,W. (1999) FEMS Microbiol. Lett., 173, 347-352.
12. Jechlinger,W., Glocker,J., Haidinger,W., Matis,A., Szostak,M.P. and Lubitz,W. (2005) J. Biotechnol., 116, 11-20.
13. Joo,H., Lin,Z. and Arnold,F.H. (1999) Nature, 399, 670-673.
14. Kloos,D.U., Stratz,M., Guttler,A., Steffan,R.J. and Timmis,K.N. (1994) J. Bacteriol., 176, 7352-7361.
15. Kouker,G. and Jaeger,K.-E. (1987) Appl. Environ. Microbiol., 53, 211-213.
16. Li,S., Xu,L.H., Hua,H., Ren,C.A. and Lin,Z.L. (2007) J. Biotechnol., 127, 647-652.
17. Madou,M., Zoval,J., Jia,G.Y., Kido,H., Kim,J. and Kim,N. (2006) Annu. Rev. Biomed. Eng., 8, 601-628.
18. Miller,J.H. (ed.) (1972) Experiments in Molecular Genetics. Cold Spring Harbor Press, New York.
19. Morita,M., Asami,K., Tanji,Y. and Unno,H. (2001) Biotechnol. Prog., 17, 573-576.
20. Mossine,V.V., Glinsky,G.V. and Feather,M.S. (1994) Carbohydr. Res., 262, 257-270.
21. Nonaka,G., Blankschien,M., Herman,C., Gross,C.A. and Rhodius,V.A. (2006) Genes Dev., 20, 1776-1789.
22. Pohn,B., Gerlach,J., Scheideler,M., Katz,H., Uray,M., Bischof,H., Klimant,I. and Schwab,H. (2007) J. Biotechnol., 129, 162-170.
23. Prim,N., Blanco,A., Martinez,J., Pastor,F.I.J. and Diaz,P. (2000) Res. Microbiol., 151, 303-312.
24. Ptashne,M., Jeffrey,A., Johnson,A.D., Maurer,R., Meyer,B.J., Pabo,C.O., Roberts,T.M. and Sauer,R.T. (1980) Cell, 19, 1-11.
25. Sakaue,R. and Kajiyama,N. (2003) Appl. Environ. Microbiol., 69, 139-145.
26. Sambrook,J. and Russell,D.W. (eds) (2001) Molecular Cloning: A Laboratory Manual, 3rd edn. Cold Spring Harbor Laboratory Press, New York.
27. Schein,C. (1989) BioTechnology, 7, 1141-1149.
28. Takahashi,M., Pischetsrieder,M. and Monnier,V.M. (1997) J. Biol. Chem., 272, 12505-12507.
29. Tanji,Y., Asami,K., Xing,X.H. and Unno,H. (1998) J. Ferment. Bioeng., 85, 74-78.
30. Turner,N.J. (2006) In Reymond,J.L. (ed.), Enzyme Assays: High-Throughput Screening, Genetic Selection and Fingerprinting. WILEY-VCH Press, Weinheim, p. 142.
31. Vasala,A., Isomaki,R., Myllykoski,L. and Alatossava,T. (1999) J. Ind. Microbiol. Biotechnol., 22, 39-43.
32. Witte,A. and Lubitz,W. (1989) Eur. J. Biochem., 180, 393-398.
33. Wong,T.S., Wu,N., Roccatano,D., Zacharias,M. and Schwaneberg,U. (2005) J. Biomol. Screen., 10, 246-252.
34. Wu,X.L., Takahashi,M., Chen,S.G. and Monnier,V.M. (2000) Biochemistry, 39, 1515-1521.
35. Xu,L.H., Li,S., Ren,C., Cai,Z. and Lin,Z.L. (2006) BioTechniques, 41, 319-322.