A versatile ligation-independent cloning method suitable for high-throughput expression screening applications

Nucleic Acids Research

Volumn 35, Issue 6, 2007, Pages

  Berrow, Nick S ^a   Alderton, David ^a   Sainsbury, Sarah ^a   Nettleship, Joanne ^a   Assenberg, Rene ^a   Rahman, Nahid ^a   Stuart, David I ^a   Owens, Raymond J ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; ENZYME; HISTIDINE; INHIBITORY GUANINE NUCLEOTIDE BINDING PROTEIN; BACTERIAL PROTEIN; HYBRID PROTEIN; KIAA0319 PROTEIN, HUMAN; NERVE PROTEIN; TRANSCRIPTION FACTOR; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; AUTOMATION; CELL STRAIN HEK293; CLONING; CONTROLLED STUDY; ESCHERICHIA COLI; EUKARYOTE; EXPRESSION VECTOR; GENE CONSTRUCT; GENE INSERTION; GENETIC TRANSFECTION; HIGH THROUGHPUT SCREENING; HOST; HOST CELL; HUMAN; HUMAN CELL; INSECT CELL; MAMMAL CELL; MOLECULAR CLONING; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN PURIFICATION; ANIMAL; BIOSYNTHESIS; CELL LINE; CHEMISTRY; GENE VECTOR; GENETICS; METABOLISM; METHODOLOGY; NEISSERIA; VALIDATION STUDY; VIRUS GENE;

EUKARYOTA; HEXAPODA; MAMMALIA;

ANIMALS; BACTERIAL PROTEINS; CELL LINE; CLONING, MOLECULAR; ESCHERICHIA COLI; GENES, VIRAL; GENETIC VECTORS; HUMANS; NEISSERIA; NERVE TISSUE PROTEINS; RECOMBINANT FUSION PROTEINS; TRANSCRIPTION FACTORS;

EID: 34247846396     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/gkm047     Document Type: Article

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