Constitutive versus thermoinducible expression of heterologous proteins in Escherichia coli based on strong PR,PL promoters from phage lambda

Biotechnology and Bioengineering

Volumn 83, Issue 2, 2003, Pages 181-190

  Menart, Viktor ^a,c   Jevševar, Simona ^a   Vilar, Mateja ^a   Trobiš, Andreja ^b   Pavko, Aleksander ^b  

^a Lek dd   (Slovenia)

^b UNIVERSITY OF LJUBLJANA   (Slovenia)

^c NATIONAL INSTITUTE OF CHEMISTRY   (Slovenia)

Author keywords

Bacteriophage lambda repressor cl857; Constitutive expression; E. coli; Tandem promoter PR,PL; Thermoinducible expression; TNF alpha analogs

Indexed keywords

ESCHERICHIA COLI; FERMENTATION; TOXIC MATERIALS;

THERMOINDUCIBLE EXPRESSION;

PROTEINS;

HETEROLOGOUS PROTEIN; PROTEIN; TOXIC SUBSTANCE; TUMOR NECROSIS FACTOR ALPHA; UNCLASSIFIED DRUG;

ARTICLE; BACTERIOPHAGE LAMBDA; BIOREACTOR; C1857 REPRESSOR GENE; CONSTITUTIVE EXPRESSION; CYTOKINE PRODUCTION; ESCHERICHIA COLI; GENE EXPRESSION SYSTEM; GENE SEGREGATION; GENETIC SELECTION; LOW TEMPERATURE; NONHUMAN; PLASMID; PROMOTER REGION; PROTEIN DEGRADATION; PROTEIN EXPRESSION; REPRESSOR GENE; TANDEM PROMOTER PR PL; THERMOINDUCIBLE EXPRESSION;

BACTERIOPHAGE LAMBDA; CLONING, MOLECULAR; ESCHERICHIA COLI; GENES, BACTERIAL; MUTAGENESIS; PLASMIDS; PROMOTER REGIONS (GENETICS); PROTEIN BIOSYNTHESIS; RECOMBINANT PROTEINS; REPRESSOR PROTEINS; RESTRICTION MAPPING; RNA, MESSENGER; TRANSCRIPTION, GENETIC; TUMOR NECROSIS FACTOR-ALPHA;

BACTERIOPHAGE LAMBDA; ESCHERICHIA COLI;

EID: 0038038942     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10660     Document Type: Article

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