Enabling IMAC purification of low abundance recombinant proteins from E. coli lysates

Nature Methods

Volumn 6, Issue 7, 2009, Pages 477-478

  Magnusdottir, Audur ^a,b   Johansson, Ida ^a   Dahlgren, Lars Göran ^a   Nordlund, Pär ^a   Berglund, Helena ^a  

^a KAROLINSKA INSTITUTE   (Sweden)

^b STOCKHOLM UNIVERSITY   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN; METAL CHELATE; NICKEL; POLYHISTIDINE TAG; RECOMBINANT PROTEIN;

BACTERIAL CELL; BINDING SITE; CELL LYSATE; CONTROLLED STUDY; ESCHERICHIA COLI; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; LETTER; METAL BINDING; MOLECULAR WEIGHT; NONHUMAN; OSMOTIC STRESS; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SEPARATION TECHNIQUE;

CHELATING AGENTS; CHROMATOGRAPHY, AFFINITY; ESCHERICHIA COLI; GREEN FLUORESCENT PROTEINS; HISTIDINE; MOLECULAR WEIGHT; NICKEL; OLIGOPEPTIDES; OSMOTIC PRESSURE; RECOMBINANT PROTEINS;

EID: 67649625535     PISSN: 15487091     EISSN: 15491676     Source Type: Journal    
DOI: 10.1038/nmeth0709-477     Document Type: Letter

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