Single-step affinity purification of recombinant proteins using the silica-binding Si-tag as a fusion partner

Protein Expression and Purification

Volumn 71, Issue 1, 2010, Pages 91-95

  Ikeda, Takeshi ^a   Ninomiya, Ken ichi ^a   Hirota, Ryuichi ^a   Kuroda, Akio ^a  

^a HIROSHIMA UNIVERSITY   (Japan)

Author keywords

Affinity purification; Affinity tag; Disordered protein; Si tag; Silanol group; Silica binding protein

Indexed keywords

HIS HIS HIS HIS HIS HIS; HIS-HIS-HIS-HIS-HIS-HIS; HISTIDINE; HYBRID PROTEIN; IMMUNOGLOBULIN G; OLIGOPEPTIDE; SILICON DIOXIDE; STAPHYLOCOCCUS PROTEIN A;

ADSORPTION; AFFINITY CHROMATOGRAPHY; ARTICLE; BIOASSAY; CHEMISTRY; ESCHERICHIA COLI; IMMUNOLOGY; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; POLYACRYLAMIDE GEL ELECTROPHORESIS;

ADSORPTION; BIOLOGICAL ASSAY; CHROMATOGRAPHY, AFFINITY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; HISTIDINE; IMMUNOGLOBULIN G; OLIGOPEPTIDES; RECOMBINANT FUSION PROTEINS; SILICON DIOXIDE; STAPHYLOCOCCAL PROTEIN A;

ESCHERICHIA COLI; STAPHYLOCOCCUS;

EID: 77049094645     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2009.12.009     Document Type: Article

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