Reduced background expression and improved plasmid stability with pET vectors in BL21 (DE3)

BioTechniques

Volumn 29, Issue 6, 2000, Pages 1234-1238

  Pan, S H ^a   Malcolm, B A ^a  

^a MERCK RESEARCH LABORATORIES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GENE PRODUCT; PLASMID VECTOR;

ARTICLE; CELL GROWTH; GENE EXPRESSION; GENE INDUCTION; GENE REPRESSION; GENETIC STABILITY; MOLECULAR CLONING; NONHUMAN;

PROKARYOTA;

EID: 0034528503     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/00296st03     Document Type: Article

References (10)

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3. Equilibrium studies of the cyclic AMP receptor protein-DNA interaction
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5. Lactose operon transcription from wild-type and L8-UV5 lac promoters in Escherichia coli treated with chloramphenicol
6. Green fluorescent protein as a quantitative reporter of relative promoter activity in E. coli
7. Use of bacteriophage T7 lysozyme to improve an inducible T7 expression system
8. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes
9. Direct selection of mutations reducing transcription or translation of the recA gene of Escherichia coli with a recA-lacZ protein fusion