High-level production of a candidacidal peptide lactoferrampin in Escherichia coli by fusion expression

Journal of Biotechnology

Volumn 139, Issue 4, 2009, Pages 326-331

  Yang, Yalin ^a   Tian, Zigang ^a   Teng, Da ^a   Zhang, Jun ^a   Wang, Jiarong ^a   Wang, Jianhua ^a  

^a INSTITUTE OF PLANT PROTECTION   (China)

Author keywords

Candidacidal peptide; Escherichia coli; Fusion expression; Lactoferrampin; On column cleavage

Indexed keywords

ANTI-MICROBIAL ACTIVITIES; ANTI-MICROBIAL PEPTIDES; CANDIDACIDAL PEPTIDE; CELLULAR PROTEINS; CODON USAGE BIAS; DNA FRAGMENTS; E. COLI; EXPRESSION LEVELS; FACTOR XA; FUSION EXPRESSION; FUSION PARTNERS; FUSION PROTEINS; HEXAHISTIDINE TAGS; HIGH-LEVEL PRODUCTIONS; LACTOFERRAMPIN; LACTOFERRIN; MALDI-TOF; MATRIX ASSISTED LASER DESORPTION IONIZATIONS; ON-COLUMN CLEAVAGE; RECOMBINANT PLASMIDS; SPECIFIC SITES; TARGET PROTEINS; THIOREDOXIN; TIME OF FLIGHTS; TOTAL SOLUBLE PROTEINS;

AFFINITY CHROMATOGRAPHY; AMINES; AMINO ACIDS; CHROMATOGRAPHIC ANALYSIS; DESORPTION; NUCLEIC ACIDS; ORGANIC ACIDS; PROTEINS;

ESCHERICHIA COLI;

BLOOD CLOTTING FACTOR 10A; DNA FRAGMENT; LACTOFERRAMPIN[265-284]; POLYPEPTIDE ANTIBIOTIC AGENT; THIOREDOXIN; UNCLASSIFIED DRUG;

ANTIFUNGAL ACTIVITY; ARTICLE; CANDIDA ALBICANS; CODON USAGE; CONTROLLED STUDY; DRUG SYNTHESIS; ESCHERICHIA COLI; GENE EXPRESSION; GENE FUSION; MOLECULAR WEIGHT; NONHUMAN; PEPTIDE SYNTHESIS; PRIORITY JOURNAL;

ANTIFUNGAL AGENTS; BASE SEQUENCE; CANDIDA ALBICANS; CHROMATOGRAPHY, AFFINITY; ESCHERICHIA COLI; LACTOFERRIN; MOLECULAR SEQUENCE DATA; PEPTIDE FRAGMENTS; RECOMBINANT FUSION PROTEINS; SEQUENCE ALIGNMENT; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

ESCHERICHIA COLI;

EID: 60849113984     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2009.01.003     Document Type: Article

References (32)

1. Arnau J., Lauritzen C., Petersen G.E., and Pedersen J. Current strategies for the use of affinity tags and tag removal for the purification of recombinant proteins. Protein Expr. Purif. 48 (2006) 1-13
2. Ausubel, F.M., Roger, B., Robert, E.K., David, D.M. (Eds.), 2005. Short Protocols in Molecular Biology, 4th ed. China Science Press, Beijing (translated by Ma, X.J., Shu, Y.L., et al., in Chinese).
3. Baneyx F. Recombinant protein expression in Escherichia coli. Curr. Opin. Biotechnol. 10 (1999) 411-421
4. Burgess-Brown N.A., Sharma S., Sobott F., Loenarz C., Oppermann U., and Gileadi O. Codon optimization can improve expression of human genes in Escherichia coli: a multi-gene study. Protein Expr. Purif. 59 (2008) 94-102
5. Cao W., Zhou Y., Ma Y., Luo Q., and Wei D. Expression and purification of antimicrobial peptide adenoregulin with C-amidated terminus in Escherichia coli. Protein Expr. Purif. 40 (2005) 404-410
6. Dian C., Eshaghi S., Urbig T., McSweeney S., Heijbel A., Salbert G., and Birse D. Strategies for the purification and on-column cleavage of glutathione-S-transferase fusion target proteins. J. Chromatogr. B: Analyt. Technol. Biomed. Life Sci. 769 (2002) 133-144
7. Einhauer A., and Jungbauer A. The FLAG™ peptide, a versatile fusion tag for the purification of recombinant proteins. J. Biochem. Biophys. Methods 49 (2001) 455-465
8. Feng X.J., Wang J.H., Shan A.S., Teng D., Yang Y.L., Yao Y., Yang G.P., Shao Y.C., Liu S., and Zhang F. Fusion expression of bovine lactoferricin in Escherichia coli. Protein Expr. Purif. 47 (2006) 110-117
9. Hannig G., and Makrides S.C. Strategies for optimizing heterologous protein expression in Escherichia coli. Trends Biotechnol. 16 (1998) 54-60
10. Huang L., Ching C.B., Jiang R., and Leong S.S. Production of bioactive human beta-defensin 5 and 6 in Escherichia coli by soluble fusion expression. Protein Expr. Purif. 61 (2008) 168-174
11. Jenny R.J., Mann K.G., and Lundblad R.L. A critical review of the methods for cleavage of fusion proteins with thrombin and factor Xa. Protein Expr. Purif. 31 (2003) 1-11
12. Kheyar A., Martin S., St-Laurent G., Timoney P.J., McCollum W.H., and Archambault D. Expression cloning and humoral immune response to the nucleocapsid and membrane proteins of equine arteritis virus. Clin. Diagn. Lab. Immunol. 4 (1997) 648-652
13. Lai Y.P., Peng Y.F., Zuo Y., Li J., Huang J., Wang L.F., and Wu Z.R. Functional and structural characterization of recombinant dermcidin-1L, a human antimicrobial peptide. Biochem. Biophys. Res. Commun. 328 (2005) 243-250
14. LaVallie E.R., DiBlasio E.A., Kovacic S., Grant K.L., Schendel P.F., and McCoy J.M. A thioredoxin gene fusion expression system that circumvents inclusion body formation in the Escherichia coli cytoplasm. Biotechnology (New York) 11 (1993) 187-193
15. Li D.H., Jian G.L., Zhang Y.T., and Ai T.M. Bacterial expression of a Trichosanthes kirilowii defensin (TDEF1) and its antifungal activity on Fusarium oxysporum. Appl. Microbiol. Biotechnol. 74 (2007) 146-151
16. Liew O.W., Ching Chong J.P., Yandle T.G., and Brennan S.O. Preparation of recombinant thioredoxin fused N-terminal proCNP: analysis of enterokinase cleavage products reveals new enterokinase cleavage sites. Protein Expr. Purif. 41 (2005) 332-340
17. Lupetti A., Brouwer C.P., Dogterom-Ballering H.E., Senesi S., Campa M., Van Dissel J.T., and Nibbering P.H. Release of calcium from intracellular stores and subsequent uptake by mitochondria are essential for the candidacidal activity of an N-terminal peptide of human lactoferrin. J. Antimicrob. Chemother. 54 (2004) 603-608
18. Miura-Ohnuma J., Nonaka T., Katoh S., Murata K., Kita A., Miki K., and Katoh E. Improved expression, purification and crystallization of a putative N-acetyl-gamma-glutamyl-phosphate reductase from rice (Oryza sativa). Acta Crystallogr. Sect. F: Struct. Biol. Cryst. Commun. 61 (2005) 1058-1061
19. Moon J.Y., Henzler-Wildman K.A., and Ramamoorthy A. Expression and purification of a recombinant LL-37 from Escherichia coli. Biochim. Biophys. Acta 1758 (2006) 1351-1358
20. 1 integrin. J. Biol. Chem. 280 (2005) 40714-40722
21. Schägger H. Tricine-SDS-PAGE. Nat. protoc. 1 (2006) 16-22
22. Shi J.H., Zhao Y.T., Zhang Y.Q., Yan Z., Han W., and Wang J.L. Study on analyzing methods of low molecular peptides with SDS-polyacrylamide gel electrophoresis. Prog. Biotech. 21 (2001) 38-41 (in Chinese)
23. Shlyapnikov Y.M., Andreev Y.A., Kozlov S.A., Vassilevski A.A., and Grishin E.V. Bacterial production of latarcin 2a, a potent antimicrobial peptide from spider venom. Protein Expr. Purif. 60 (2008) 89-95
24. Sørensen H.P., and Mortensen K.K. Advanced genetic strategies for recombinant protein expression in Escherichia coli. J. Biotechnol. 115 (2005) 113-128
25. Srinivasulu B., Syvitski R., Seo J.K., Mattatall N.R., Knickle L.C., and Douglas S.E. Expression, purification and structural characterization of recombinant hepcidin, an antimicrobial peptide identified in Japanese flounder, Paralichthys olivaceus. Protein Expr. Purif. 61 (2008) 36-44
26. Tenno T., Goda N., Tateishi Y., Tochio H., Mishima M., Hayashi H., Shirakawa M., and Hiroaki H. High-throughput construction method for expression vector of peptides for NMR study suited for isotopic labeling. Protein Eng. Des. Sel. 17 (2004) 305-314
27. van der Kraan M.I., Groenink J., Nazmi K., Veerman E.C., Bolscher J.G., and Nieuw Amerongen A.V. Lactoferrampin: a novel antimicrobial peptide in the N1-domain of bovine lactoferrin. Peptides 25 (2004) 177-183
28. van der Kraan M.I., Nazmi K., Teeken A., Groenink J., van't Hof W., Veerman E.C., Bolscher J.G., and Nieuw Amerongen A.V. Lactoferrampin, an antimicrobial peptide of bovine lactoferrin, exerts its candidacidal activity by a cluster of positively charged residues at the C-terminus in combination with a helix-facilitating N-terminal part. Biol. Chem. 386 (2005) 137-142
29. Xu Z., Zhong Z., Huang L., Peng L., Wang F., and Cen P. High-level production of bioactive human beta-defensin-4 in Escherichia coli by soluble fusion expression. Appl. Microbiol. Biotechnol. 72 (2006) 471-479
30. Yang Y.L., Luo J., Wang J.H., Teng D., and Tian Z.G. Expression and characterization of dextransucrase gene dsrX from Leuconostoc mesenteroides in Escherichia coli. J. Biotechnol. 133 (2008) 505-512
31. Zheng W., Yang F., Wu F., Lu C., and Hua Z. Purification of cadmium ion binding metallothionein-3 by proteinase digestion on affinity chromatographic column. Chin. J. Chromatogr. 24 (2006) 279-283
32. Zhou Q.F., Luo X.G., Ye L., and Xi T. High-level production of a novel antimicrobial peptide perinerin in Escherichia coli by fusion expression. Curr. Microbiol. 54 (2007) 366-370