Over-expression of proteins using a modified pBAD24 vector in E. coli expression system

Biotechnology Letters

Volumn 31, Issue 7, 2009, Pages 1031-1036

  Banerjee, Sampali ^a   Salunkhe, Shardul S ^a   Apte Deshpande, Anjali D ^a   Mandi, Naganath S ^a   Mandal, Goutam ^a   Padmanabhan, Sriram ^a  

^a LUPIN LIMITED   (India)

Author keywords

Granulocyte colony stimulating factor; PBAD24; Shine Dalgarno sequence; Staphylokinase; T7g10 sequence

Indexed keywords

GRANULOCYTE COLONY STIMULATING FACTOR; PBAD24; SHINE-DALGARNO SEQUENCE; STAPHYLOKINASE; T7G10 SEQUENCE;

ESCHERICHIA COLI; PROTEINS;

VECTORS;

RECOMBINANT PROTEIN;

ARTICLE; BACTERIAL GENE; BACTERIOPHAGE; BIOSYNTHESIS; ESCHERICHIA COLI; GENE EXPRESSION; GENE VECTOR; GENETICS; METABOLISM; PROMOTER REGION; VIRUS GENE;

ESCHERICHIA COLI; GENE EXPRESSION; GENES, BACTERIAL; GENES, VIRAL; GENETIC VECTORS; PODOVIRIDAE; PROMOTER REGIONS, GENETIC; RECOMBINANT PROTEINS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; PROKARYOTA;

EID: 67649211351     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-009-9976-6     Document Type: Article

References (14)

1. JE Cronan 2006 A family of arabinose-inducible Escherichia coli expression vectors having pBR322 copy control Plasmid 55 152 157 (Pubitemid 43202106)
2. FC Golich M Han MW Crowder 2006 Over-expression, purification, and characterization of aminopeptidase N from Escherichia coli Protein Expr Purif 47 634 639 (Pubitemid 43729023)
3. LM Guzman D Belin MJ Carson J Beckwith 1995 Tight regulation, modulation, and high-level expression by vectors containing the arabinose pBAD promoter J Bacteriol 177 4121 4130
4. WF Jacob M Santer AE Dahlberg 1987 A single-base change in the Shine-Dalgarno region of 16S rRNA of Escherichia coli affects translation of many proteins Proc Natl Acad Sci USA 84 4757 4761
5. H Jin Q Zhao V de Gonzalez DH Ardell M Stenstrom LA Isaksson 2006 Influences on gene expression in vivo by a Shine-Dalgarno sequence Mol Microbiol 60 480 492
6. N Mandi S Soorapaneni S Rewanwar P Kotwal B Prasad G Mandal S Padmanabhan 2009 High yielding recombinant staphylokinase in bacterial expression system-cloning, expression, purification and activity studies Protein Expr Purif 64 69 75
7. JR Newman C Fuqua 1999 Broad-host-range expression vectors that carry the l-arabinose-inducible Escherichia coli araBAD promoter and the araC regulator Gene 227 197 203 (Pubitemid 29104855)
8. M O'Connor AE Dhalberg 2001 Enhancement of translation by the epsilon element is independent of the sequence of the 460 region of 16 s rRNA Nucleic Acids Res 29 1420 1425 (Pubitemid 32288487)
9. Y Oku K Kurokawa N Ichihashi K Sekimizu 2004 Characterization of the Staphylococcus aureus mprF gene, involved in lysinylation of phosphatidylglycerol Microbiology 150 45 51 (Pubitemid 38111469)
10. PO Olins SH Rangwala 1989 A novel sequence element derived from bacteriophage T7 mRNA acts as an enhancer of translation of the lacZ gene in Escherichia coli J Biol Chem 264 16973 16976 (Pubitemid 19255522)
11. J Shine L Dalgarno 1974 The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites Proc Natl Acad Sci USA 71 1342 1346
12. R Sukhchawalit P Vattanaviboon R Sallabhan S Mongkolsuk 1999 Construction and characterization of regulated l-arabinose-inducible broad host range expression vectors in Xanthomonas FEMS Microbiol Lett 181 217 223
13. R Walia J Deb KJ Mukherjee 2008 Stability studies with different vector backbones utilizing the T7 expression system in Escherichia coli J Chem Technol Biotechnol 83 1120 1125
14. X Wu H Jörnvall KD Berndt U Oppermann 2004 Codon optimization reveals critical factors for high level expression of two rare codon genes in Escherichia coli: RNA stability and secondary structure but not tRNA abundance Biochem Biophys Res Commun 313 89 96 (Pubitemid 37524029)