Soluble expression in Escherichia coli of active human cyclic nucleotide phosphodiesterase isoform 4B2 in fusion with maltose-binding protein

Bioscience, Biotechnology and Biochemistry

Volumn 73, Issue 4, 2009, Pages 968-970

  Zhu, Sha ^a   Yang, Genqing ^a,b   Yang, Xiaolan ^a   Zhao, Yunsheng ^a   Li, Xiang ^a   Deng, Ping ^a   Xie, Yanling ^a   Gan, Zhiyong ^a   Liu, Yin ^a   Li, Zhirong ^a   Liao, Juan ^a   Yu, Ming'an ^a   Liao, Fei ^a  

^a CHONGQING MEDICAL UNIVERSITY   (China)

^b XINXIANG MEDICAL UNIVERSITY   (China)

Author keywords

Cyclic nucleotide phosphodiesterase 4; Kinetic properties; Maltose binding protein; Recombinant expression; Rolipram

Indexed keywords

ACTIVE PROTEINS; CYCLIC NUCLEOTIDE; CYCLIC NUCLEOTIDE PHOSPHODIESTERASE 4; DISSOCIATION CONSTANT; E. COLI; ISOFORM; KINETIC PROPERTIES; MALTOSE BINDING PROTEINS; MICHAELIS-MENTEN CONSTANT; PHOSPHODIESTERASE; PHOSPHODIESTERASE-4; POTENT INHIBITOR; RECOMBINANT EXPRESSION; ROLIPRAM; SOLUBLE EXPRESSION; U937 CELL; ZINC IONS;

CHROMATOGRAPHIC ANALYSIS; DISSOCIATION; ESCHERICHIA COLI; MALTOSE; NUCLEIC ACIDS; RESINS; ZINC;

NUCLEOTIDES;

CARRIER PROTEIN; CELL EXTRACT; HYBRID PROTEIN; ISOENZYME; ISOPROPYL THIOGALACTOSIDE; MALTOSE BINDING PROTEIN; MALTOSE-BINDING PROTEIN; PHOSPHODIESTERASE IV;

ANIMAL; ARTICLE; BIOSYNTHESIS; CHEMISTRY; DRUG EFFECT; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; HUMAN; ISOLATION AND PURIFICATION; SOLUBILITY; TUMOR CELL LINE;

ANIMALS; CARRIER PROTEINS; CELL EXTRACTS; CELL LINE, TUMOR; CYCLIC NUCLEOTIDE PHOSPHODIESTERASES, TYPE 4; ESCHERICHIA COLI; GENE EXPRESSION; HUMANS; ISOENZYMES; ISOPROPYL THIOGALACTOSIDE; RECOMBINANT FUSION PROTEINS; SOLUBILITY;

ESCHERICHIA COLI;

EID: 67149131561     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.90131     Document Type: Article

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