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Volumn , Issue 6, 2010, Pages

RNAi screens for the identification and validation of novel targets: Current status and challenges

Author keywords

[No Author keywords available]

Indexed keywords

REAGENT;

EID: 79952319821     PISSN: 13608606     EISSN: None     Source Type: Trade Journal    
DOI: None     Document Type: Review
Times cited : (2)

References (77)
  • 1
    • 0037686295 scopus 로고    scopus 로고
    • Killing the messenger: Short RNAs that silence gene expression
    • Dykxhoorn, D. M., Novina, C. D. & Sharp, P. A. Killing the messenger: short RNAs that silence gene expression. Nat Rev Mol Cell Biol 4, 457-467 (2003)
    • (2003) Nat Rev Mol Cell Biol , vol.4 , pp. 457-467
    • Dykxhoorn, D.M.1    Novina, C.D.2    Sharp, P.A.3
  • 2
    • 4644223259 scopus 로고    scopus 로고
    • Mechanisms of gene silencing by double-stranded RNA
    • Meister, G. & Tuschl, T. Mechanisms of gene silencing by double-stranded RNA. Nature 431, 343-349 (2004)
    • (2004) Nature , vol.431 , pp. 343-349
    • Meister, G.1    Tuschl, T.2
  • 3
    • 24644446398 scopus 로고    scopus 로고
    • Ribo-gnome: The big world of small RNAs
    • Zamore, P. D. & Haley, B. Ribo-gnome: the big world of small RNAs. Science 309, 1519-1524 (2005)
    • (2005) Science , vol.309 , pp. 1519-1524
    • Zamore, P.D.1    Haley, B.2
  • 4
    • 35348996554 scopus 로고    scopus 로고
    • Specialization and evolution of endogenous small RNA pathways
    • doi:nrg2179 [pii] 10.1038/nrg2179
    • Chapman, E. J. & Carrington, J. C. Specialization and evolution of endogenous small RNA pathways. Nat Rev Genet 8, 884-896, doi:nrg2179 [pii] 10.1038/nrg2179 (2007)
    • (2007) Nat Rev Genet , vol.8 , pp. 884-896
    • Chapman, E.J.1    Carrington, J.C.2
  • 5
    • 59349116903 scopus 로고    scopus 로고
    • Knocking down barriers: Advances in siRNA delivery
    • doi:nrd2742 [pii] 10.1038/nrd2742
    • Whitehead, K. A., Langer, R. & Anderson, D. G. Knocking down barriers: advances in siRNA delivery. Nat Rev Drug Discov 8, 129-138, doi:nrd2742 [pii] 10.1038/nrd2742 (2009)
    • (2009) Nat Rev Drug Discov , vol.8 , pp. 129-138
    • Whitehead, K.A.1    Langer, R.2    Anderson, D.G.3
  • 6
    • 68549122864 scopus 로고    scopus 로고
    • RNA interference screening for the discovery of oncology targets
    • doi:10.1517/14728220903179338
    • Quon, K. & Kassner, P. D. RNA interference screening for the discovery of oncology targets. Expert Opin Ther Targets 13, 1027-1035, doi:10.1517/14728220903179338 (2009)
    • (2009) Expert Opin Ther Targets , vol.13 , pp. 1027-1035
    • Quon, K.1    Kassner, P.D.2
  • 7
    • 37549040566 scopus 로고    scopus 로고
    • RNA interference-mediated inhibition of Semliki Forest virus replication in mammalian cells
    • doi:10.1089/oli.2007.0079
    • Seyhan, A. A., Alizadeh, B. N., Lundstrom, K. & Johnston, B. H. RNA interference-mediated inhibition of Semliki Forest virus replication in mammalian cells. Oligonucleotides 17, 473-484, doi:10.1089/oli.2007.0079 (2007)
    • (2007) Oligonucleotides , vol.17 , pp. 473-484
    • Seyhan, A.A.1    Alizadeh, B.N.2    Lundstrom, K.3    Johnston, B.H.4
  • 8
    • 17844407907 scopus 로고    scopus 로고
    • Complete, gene-specific siRNA libraries: Production and expression in mammalian cells
    • doi:11/5/837 [pii] 10.1261/rna.7285805
    • Seyhan, A. A. et al. Complete, gene-specific siRNA libraries: production and expression in mammalian cells. RNA 11, 837-846, doi:11/5/837 [pii] 10.1261/rna.7285805 (2005)
    • (2005) RNA , vol.11 , pp. 837-846
    • Seyhan, A.A.1
  • 9
    • 33646187810 scopus 로고    scopus 로고
    • Highthroughput RNAi screening in cultured cells: A user's guide
    • doi:nrg1836 [pii] 10.1038/nrg1836
    • Echeverri, C. J. & Perrimon, N. Highthroughput RNAi screening in cultured cells: a user's guide. Nat Rev Genet 7, 373-384, doi:nrg1836 [pii] 10.1038/nrg1836 (2006)
    • (2006) Nat Rev Genet , vol.7 , pp. 373-384
    • Echeverri, C.J.1    Perrimon, N.2
  • 10
    • 78649286116 scopus 로고    scopus 로고
    • RNAi Screening for the Discovery of Novel Modulators of Human Disease
    • doi:BSP/CPB/EPub/ 0077-11-5 [pii]
    • Seyhan, A. A. & Ryan, T. E. RNAi Screening for the Discovery of Novel Modulators of Human Disease. Curr Pharm Biotechnol, doi:BSP/CPB/EPub/ 0077-11-5 [pii] (2010)
    • (2010) Curr Pharm Biotechnol
    • Seyhan, A.A.1    Ryan, T.E.2
  • 11
    • 34347391035 scopus 로고    scopus 로고
    • Utilizing RNA interference to enhance cancer drug discovery
    • doi:nrd2355 [pii] 10.1038/nrd2355
    • Iorns, E., Lord, C. J., Turner, N. & Ashworth, A. Utilizing RNA interference to enhance cancer drug discovery. Nat Rev Drug Discov 6, 556-568, doi:nrd2355 [pii] 10.1038/nrd2355 (2007)
    • (2007) Nat Rev Drug Discov , vol.6 , pp. 556-568
    • Iorns, E.1    Lord, C.J.2    Turner, N.3    Ashworth, A.4
  • 12
    • 45549089565 scopus 로고    scopus 로고
    • The art and design of genetic screens: RNA interference
    • doi:nrg2364 [pii] 10.1038/nrg2364
    • Boutros, M. & Ahringer, J. The art and design of genetic screens: RNA interference. Nat Rev Genet 9, 554-566, doi:nrg2364 [pii] 10.1038/nrg2364 (2008)
    • (2008) Nat Rev Genet , vol.9 , pp. 554-566
    • Boutros, M.1    Ahringer, J.2
  • 13
    • 77953626646 scopus 로고    scopus 로고
    • Genomic screening with RNAi: Results and challenges
    • doi:10.1146/annurev-biochem-060408- 092949
    • Mohr, S., Bakal, C. & Perrimon, N. Genomic screening with RNAi: results and challenges. Annu Rev Biochem 79, 37-64, doi:10.1146/annurev-biochem- 060408- 092949 (2010)
    • (2010) Annu Rev Biochem , vol.79 , pp. 37-64
    • Mohr, S.1    Bakal, C.2    Perrimon, N.3
  • 14
    • 33646188259 scopus 로고    scopus 로고
    • 3′ UTR seed matches, but not overall identity, are associated with RNAi off-targets
    • doi:nmeth854 [pii] 10.1038/nmeth854
    • Birmingham, A. et al. 3′ UTR seed matches, but not overall identity, are associated with RNAi off-targets. Nat Methods 3, 199-204, doi:nmeth854 [pii] 10.1038/nmeth854 (2006)
    • (2006) Nat Methods , vol.3 , pp. 199-204
    • Birmingham, A.1
  • 15
    • 33745612464 scopus 로고    scopus 로고
    • Widespread siRNA "off-target" transcript silencing mediated by seed region sequence complementarity
    • doi:rna.25706 [pii] 10.1261/rna.25706
    • Jackson, A. L. et al. Widespread siRNA "off-target" transcript silencing mediated by seed region sequence complementarity. RNA 12, 1179-1187, doi:rna.25706 [pii] 10.1261/rna.25706 (2006)
    • (2006) RNA , vol.12 , pp. 1179-1187
    • Jackson, A.L.1
  • 16
    • 22344454846 scopus 로고    scopus 로고
    • Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA
    • doi:nbt1081 [pii] 10.1038/nbt1081
    • Judge, A. D. et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol 23, 457-462, doi:nbt1081 [pii] 10.1038/nbt1081 (2005)
    • (2005) Nat Biotechnol , vol.23 , pp. 457-462
    • Judge, A.D.1
  • 17
    • 23344438420 scopus 로고    scopus 로고
    • siRNA-mediated off-target gene silencing triggered by a 7 nt complementation
    • doi:33/14/4527 [pii] 10.1093/nar/gki762
    • Lin, X. et al. siRNA-mediated off-target gene silencing triggered by a 7 nt complementation. Nucleic Acids Res 33, 4527-4535, doi:33/14/4527 [pii] 10.1093/nar/gki762 (2005)
    • (2005) Nucleic Acids Res , vol.33 , pp. 4527-4535
    • Lin, X.1
  • 18
    • 10644284795 scopus 로고    scopus 로고
    • RNA interference and double-stranded-RNA-activated pathways
    • doi:BST0320952 [pii] 10.1042/BST0320952
    • Sledz, C. A. & Williams, B. R. RNA interference and double-stranded-RNA-activated pathways. Biochem Soc Trans 32, 952-956, doi:BST0320952 [pii] 10.1042/BST0320952 (2004)
    • (2004) Biochem Soc Trans , vol.32 , pp. 952-956
    • Sledz, C.A.1    Williams, B.R.2
  • 19
    • 77950668281 scopus 로고    scopus 로고
    • High-throughput RNAi screening to dissect cellular pathways: A how-to guide
    • doi:10.1002/biot.200900277
    • Falschlehner, C., Steinbrink, S., Erdmann, G. & Boutros, M. High-throughput RNAi screening to dissect cellular pathways: a how-to guide. Biotechnol J 5, 368-376, doi:10.1002/biot.200900277 (2010)
    • (2010) Biotechnol J , vol.5 , pp. 368-376
    • Falschlehner, C.1    Steinbrink, S.2    Erdmann, G.3    Boutros, M.4
  • 20
    • 33747776448 scopus 로고    scopus 로고
    • shRNA libraries and their use in cancer genetics
    • doi:nmeth921 [pii] 10.1038/ nmeth921
    • Bernards, R., Brummelkamp, T. R. & Beijersbergen, R. L. shRNA libraries and their use in cancer genetics. Nat Methods 3, 701-706, doi:nmeth921 [pii] 10.1038/ nmeth921 (2006)
    • (2006) Nat Methods , vol.3 , pp. 701-706
    • Bernards, R.1    Brummelkamp, T.R.2    Beijersbergen, R.L.3
  • 21
    • 0141750434 scopus 로고    scopus 로고
    • Identification of modulators of TRAIL-induced apoptosis via RNAi-based phenotypic screening
    • doi:S1097276503003484 [pii]
    • Aza-Blanc, P. et al. Identification of modulators of TRAIL-induced apoptosis via RNAi-based phenotypic screening. Mol Cell 12, 627-637, doi:S1097276503003484 [pii] (2003)
    • (2003) Mol Cell , vol.12 , pp. 627-637
    • Aza-Blanc, P.1
  • 22
    • 77955655940 scopus 로고    scopus 로고
    • Identification of WEE1 as a potential molecular target in cancer cells by RNAi screening of the human tyrosine kinome
    • doi:10.1007/s10549-009-0571-2
    • Murrow, L. M., Garimella, S. V., Jones, T. L., Caplen, N. J. & Lipkowitz, S. Identification of WEE1 as a potential molecular target in cancer cells by RNAi screening of the human tyrosine kinome. Breast Cancer Res Treat, doi:10.1007/s10549-009-0571-2 (2009)
    • (2009) Breast Cancer Res Treat
    • Murrow, L.M.1    Garimella, S.V.2    Jones, T.L.3    Caplen, N.J.4    Lipkowitz, S.5
  • 24
    • 35148885729 scopus 로고    scopus 로고
    • A functional genetic approach identifies the PI3K pathway as a major determinant of trastuzumab resistance in breast cancer
    • doi:S1535-6108(07)00262-0 [pii] 10.1016/j.ccr.2007.08.030
    • Berns, K. et al. A functional genetic approach identifies the PI3K pathway as a major determinant of trastuzumab resistance in breast cancer. Cancer Cell 12, 395-402, doi:S1535-6108(07)00262-0 [pii] 10.1016/j.ccr.2007.08. 030 (2007)
    • (2007) Cancer Cell , vol.12 , pp. 395-402
    • Berns, K.1
  • 25
    • 33646343474 scopus 로고    scopus 로고
    • An shRNA barcode screen provides insight into cancer cell vulnerability to MDM2 inhibitors
    • doi:nchembio774 [pii] 10.1038/nchembio774
    • Brummelkamp, T. R. et al. An shRNA barcode screen provides insight into cancer cell vulnerability to MDM2 inhibitors. Nat Chem Biol 2, 202-206, doi:nchembio774 [pii] 10.1038/nchembio774 (2006)
    • (2006) Nat Chem Biol , vol.2 , pp. 202-206
    • Brummelkamp, T.R.1
  • 26
    • 20444426889 scopus 로고    scopus 로고
    • A genetic screen identifies PITX1 as a suppressor of RAS activity and tumorigenicity
    • doi:S0092-8674(05)00397-1 [pii] 10.1016/j.cell.2005.04.017
    • Kolfschoten, I. G. et al. A genetic screen identifies PITX1 as a suppressor of RAS activity and tumorigenicity. Cell 121, 849-858, doi:S0092-8674(05)00397-1 [pii] 10.1016/j.cell.2005.04.017 (2005)
    • (2005) Cell , vol.121 , pp. 849-858
    • Kolfschoten, I.G.1
  • 27
    • 58149521944 scopus 로고    scopus 로고
    • Highly parallel identification of essential genes in cancer cells
    • doi:0810485105 [pii] 10.1073/pnas.0810485105
    • Luo, B. et al. Highly parallel identification of essential genes in cancer cells. Proc Natl Acad Sci U S A 105, 20380-20385, doi:0810485105 [pii] 10.1073/pnas.0810485105 (2008)
    • (2008) Proc Natl Acad Sci U S A , vol.105 , pp. 20380-20385
    • Luo, B.1
  • 28
    • 33645306041 scopus 로고    scopus 로고
    • A loss-of-function RNA interference screen for molecular targets in cancer
    • doi:nature04687 [pii] 10.1038/nature04687
    • Ngo, V. N. et al. A loss-of-function RNA interference screen for molecular targets in cancer. Nature 441, 106-110, doi:nature04687 [pii] 10.1038/nature04687 (2006).
    • (2006) Nature , vol.441 , pp. 106-110
    • Ngo, V.N.1
  • 29
    • 38849098442 scopus 로고    scopus 로고
    • Cancer proliferation gene discovery through functional genomics
    • doi:319/5863/620 [pii] 10.1126/science.1149200
    • Schlabach, M. R. et al. Cancer proliferation gene discovery through functional genomics. Science 319, 620-624, doi:319/5863/620 [pii] 10.1126/science.1149200 (2008)
    • (2008) Science , vol.319 , pp. 620-624
    • Schlabach, M.R.1
  • 30
    • 38849192515 scopus 로고    scopus 로고
    • Profiling essential genes in human mammary cells by multiplex RNAi screening
    • doi:319/5863/617 [pii] 10.1126/science. 1149185
    • Silva, J. M. et al. Profiling essential genes in human mammary cells by multiplex RNAi screening. Science 319, 617-620, doi:319/5863/617 [pii] 10.1126/science. 1149185 (2008)
    • (2008) Science , vol.319 , pp. 617-620
    • Silva, J.M.1
  • 31
    • 20444376939 scopus 로고    scopus 로고
    • A genetic screen for candidate tumor suppressors identifies REST
    • doi:S0092-8674(05)00350-8 [pii] 10.1016/j.cell.2005.03.033
    • Westbrook, T. F. et al. A genetic screen for candidate tumor suppressors identifies REST. Cell 121, 837-848, doi:S0092-8674(05)00350-8 [pii] 10.1016/j.cell.2005.03.033 (2005)
    • (2005) Cell , vol.121 , pp. 837-848
    • Westbrook, T.F.1
  • 32
    • 33747794587 scopus 로고    scopus 로고
    • Genome-scale lossof- function screening with a lentiviral RNAi library
    • doi:nmeth924 [pii] 10.1038/nmeth924
    • Root, D. E., Hacohen, N., Hahn, W. C., Lander, E. S. & Sabatini, D. M. Genome-scale lossof- function screening with a lentiviral RNAi library. Nat Methods 3, 715-719, doi:nmeth924 [pii] 10.1038/nmeth924 (2006)
    • (2006) Nat Methods , vol.3 , pp. 715-719
    • Root, D.E.1    Hacohen, N.2    Hahn, W.C.3    Lander, E.S.4    Sabatini, D.M.5
  • 33
    • 9144260199 scopus 로고    scopus 로고
    • Use of RNA interference libraries to investigate oncogenic signalling in mammalian cells
    • doi:1208073 [pii] 10.1038/sj.onc.1208073
    • Downward, J. Use of RNA interference libraries to investigate oncogenic signalling in mammalian cells. Oncogene 23, 8376-8383, doi:1208073 [pii] 10.1038/sj.onc.1208073 (2004)
    • (2004) Oncogene , vol.23 , pp. 8376-8383
    • Downward, J.1
  • 35
    • 65849111219 scopus 로고    scopus 로고
    • Synthetic lethal interaction between oncogenic KRAS dependency and STK33 suppression in human cancer cells
    • doi:S0092-8674(09)00316- X [pii] 10.1016/j.cell.2009.03.017
    • Scholl, C. et al. Synthetic lethal interaction between oncogenic KRAS dependency and STK33 suppression in human cancer cells. Cell 137, 821-834, doi:S0092-8674(09)00316- X [pii] 10.1016/j.cell.2009.03.017 (2009)
    • (2009) Cell , vol.137 , pp. 821-834
    • Scholl, C.1
  • 36
    • 34547107098 scopus 로고    scopus 로고
    • RNAi screen identifies UBE2D3 as a mediator of all-trans retinoic acid-induced cell growth arrest in human acute promyelocytic NB4 cells
    • doi:blood-2006-11-059048 [pii] 10.1182/blood-2006-11-059048
    • Hattori, H. et al. RNAi screen identifies UBE2D3 as a mediator of all-trans retinoic acid-induced cell growth arrest in human acute promyelocytic NB4 cells. Blood 110, 640-650, doi:blood-2006-11-059048 [pii] 10.1182/blood-2006-11-059048 (2007)
    • (2007) Blood , vol.110 , pp. 640-650
    • Hattori, H.1
  • 37
    • 34147198467 scopus 로고    scopus 로고
    • Synthetic lethal screen identification of chemosensitizer loci in cancer cells
    • doi:nature05697 [pii] 10.1038/nature05697
    • Whitehurst, A. W. et al. Synthetic lethal screen identification of chemosensitizer loci in cancer cells. Nature 446, 815-819, doi:nature05697 [pii] 10.1038/nature05697 (2007)
    • (2007) Nature , vol.446 , pp. 815-819
    • Whitehurst, A.W.1
  • 38
    • 45749102122 scopus 로고    scopus 로고
    • Cancer: Deconstructing oncogenesis
    • doi:453995a [pii] 10.1038/453995a
    • Luo, J. & Elledge, S. J. Cancer: Deconstructing oncogenesis. Nature 453, 995-996, doi:453995a [pii] 10.1038/453995a (2008)
    • (2008) Nature , vol.453 , pp. 995-996
    • Luo, J.1    Elledge, S.J.2
  • 39
    • 39349097864 scopus 로고    scopus 로고
    • Identification of host proteins required for HIV infection through a functional genomic screen
    • doi:1152725 [pii] 10.1126/science.1152725
    • Brass, A. L. et al. Identification of host proteins required for HIV infection through a functional genomic screen. Science 319, 921-926, doi:1152725 [pii] 10.1126/science.1152725 (2008)
    • (2008) Science , vol.319 , pp. 921-926
    • Brass, A.L.1
  • 40
    • 55249088255 scopus 로고    scopus 로고
    • Genome-scale RNAi screen for host factors required for HIV replication
    • doi:S1931-3128(08)00330-2 [pii] 10.1016/j.chom.2008.10.004
    • Zhou, H. et al. Genome-scale RNAi screen for host factors required for HIV replication. Cell Host Microbe 4, 495-504, doi:S1931-3128(08)00330-2 [pii] 10.1016/j.chom.2008.10.004 (2008)
    • (2008) Cell Host Microbe , vol.4 , pp. 495-504
    • Zhou, H.1
  • 41
    • 70349513508 scopus 로고    scopus 로고
    • A genome-wide genetic screen for host factors required for hepatitis C virus propagation
    • doi:0907439106 [pii] 10.1073/pnas.0907439106
    • Li, Q. et al. A genome-wide genetic screen for host factors required for hepatitis C virus propagation. Proc Natl Acad Sci U S A 106, 16410-16415, doi:0907439106 [pii] 10.1073/pnas.0907439106 (2009)
    • (2009) Proc Natl Acad Sci U S A , vol.106 , pp. 16410-16415
    • Li, Q.1
  • 42
    • 61849160363 scopus 로고    scopus 로고
    • A functional genomic screen identifies cellular cofactors of hepatitis C virus replication
    • doi:S1931-3128(09)00061-4 [pii] 10.1016/j.chom.2009.02.001
    • Tai, A. W. et al. A functional genomic screen identifies cellular cofactors of hepatitis C virus replication. Cell Host Microbe 5, 298-307, doi:S1931-3128(09)00061-4 [pii] 10.1016/j.chom.2009.02.001 (2009)
    • (2009) Cell Host Microbe , vol.5 , pp. 298-307
    • Tai, A.W.1
  • 43
    • 34547913440 scopus 로고    scopus 로고
    • Cellular cofactors affecting hepatitis C virus infection and replication
    • doi:0704894104 [pii] 10.1073/pnas.0704894104
    • Randall, G. et al. Cellular cofactors affecting hepatitis C virus infection and replication. Proc Natl Acad Sci U S A 104, 12884-12889, doi:0704894104 [pii] 10.1073/pnas.0704894104 (2007)
    • (2007) Proc Natl Acad Sci U S A , vol.104 , pp. 12884-12889
    • Randall, G.1
  • 44
    • 49649127852 scopus 로고    scopus 로고
    • Drosophila RNAi screen identifies host genes important for influenza virus replication
    • doi:nature07151 [pii] 10.1038/nature07151
    • Hao, L. et al. Drosophila RNAi screen identifies host genes important for influenza virus replication. Nature 454, 890-893, doi:nature07151 [pii] 10.1038/nature07151 (2008)
    • (2008) Nature , vol.454 , pp. 890-893
    • Hao, L.1
  • 45
    • 51649095289 scopus 로고    scopus 로고
    • RNA interference screen for human genes associated with West Nile virus infection
    • doi:nature07207 [pii] 10.1038/nature07207
    • Krishnan, M. N. et al. RNA interference screen for human genes associated with West Nile virus infection. Nature 455, 242-245, doi:nature07207 [pii] 10.1038/nature07207 (2008)
    • (2008) Nature , vol.455 , pp. 242-245
    • Krishnan, M.N.1
  • 46
    • 48249132855 scopus 로고    scopus 로고
    • RNAi screen of endoplasmic reticulum-associated host factors reveals a role for IRE1alpha in supporting Brucella replication
    • doi:10.1371/journal.ppat.1000110
    • Qin, Q. M. et al. RNAi screen of endoplasmic reticulum-associated host factors reveals a role for IRE1alpha in supporting Brucella replication. PLoS Pathog 4, e1000110, doi:10.1371/journal.ppat.1000110 (2008)
    • (2008) PLoS Pathog , vol.4
    • Qin, Q.M.1
  • 47
    • 33644519040 scopus 로고    scopus 로고
    • Building mammalian signalling pathways with RNAi screens
    • doi:nrm1860 [pii] 10.1038/nrm1860
    • Moffat, J. & Sabatini, D. M. Building mammalian signalling pathways with RNAi screens. Nat Rev Mol Cell Biol 7, 177-187, doi:nrm1860 [pii] 10.1038/nrm1860 (2006)
    • (2006) Nat Rev Mol Cell Biol , vol.7 , pp. 177-187
    • Moffat, J.1    Sabatini, D.M.2
  • 48
    • 33846466430 scopus 로고    scopus 로고
    • Applications of high-throughput RNA interference screens to problems in cell and developmental biology
    • doi:175/1/7 [pii] 10.1534/genetics.106.069963
    • Perrimon, N. & Mathey-Prevot, B. Applications of high-throughput RNA interference screens to problems in cell and developmental biology. Genetics 175, 7-16, doi:175/1/7 [pii] 10.1534/genetics.106.069963 (2007)
    • (2007) Genetics , vol.175 , pp. 7-16
    • Perrimon, N.1    Mathey-Prevot, B.2
  • 49
    • 30944440768 scopus 로고    scopus 로고
    • High-throughput screening of effective siRNAs from RNAi libraries delivered via bacterial invasion
    • doi:nmeth812 [pii] 10.1038/nmeth812
    • Zhao, H. F. et al. High-throughput screening of effective siRNAs from RNAi libraries delivered via bacterial invasion. Nat Methods 2, 967-973, doi:nmeth812 [pii] 10.1038/nmeth812 (2005)
    • (2005) Nat Methods , vol.2 , pp. 967-973
    • Zhao, H.F.1
  • 50
    • 0042972933 scopus 로고    scopus 로고
    • Activation of the interferon system by short-interfering RNAs
    • DOI 10.1038/ncb1038
    • Sledz, C. A., Holko, M., de Veer, M. J., Silverman, R. H. & Williams, B. R. Activation of the interferon system by short-interfering RNAs. Nat Cell Biol 5, 834-839, doi:10.1038/ncb1038 ncb1038 [pii] (2003) (Pubitemid 37087172)
    • (2003) Nature Cell Biology , vol.5 , Issue.9 , pp. 834-839
    • Sledz, C.A.1    Holko, M.2    De Veer, M.J.3    Silverman, R.H.4    Williams, B.R.G.5
  • 51
    • 11144282529 scopus 로고    scopus 로고
    • Role of Toll-like receptors in antisense and siRNA
    • corrected doi:nbt1042 [pii] 10.1038/nbt1042
    • Agrawal, S. & Kandimalla, E. R. Role of Toll-like receptors in antisense and siRNA [corrected]. Nat Biotechnol 22, 1533-1537, doi:nbt1042 [pii] 10.1038/nbt1042 (2004)
    • (2004) Nat Biotechnol , vol.22 , pp. 1533-1537
    • Agrawal, S.1    Kandimalla, E.R.2
  • 52
    • 67651154351 scopus 로고    scopus 로고
    • RNAi screening: Tips and techniques
    • doi:ni0809-799 [pii] 10.1038/ni0809-799
    • Sharma, S. & Rao, A. RNAi screening: tips and techniques. Nat Immunol 10, 799-804, doi:ni0809-799 [pii] 10.1038/ni0809-799 (2009)
    • (2009) Nat Immunol , vol.10 , pp. 799-804
    • Sharma, S.1    Rao, A.2
  • 53
    • 0033003760 scopus 로고    scopus 로고
    • A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays
    • Zhang, J. H., Chung, T. D. & Oldenburg, K. R. A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays. J Biomol Screen 4, 67-73 (1999)
    • (1999) J Biomol Screen , vol.4 , pp. 67-73
    • Zhang, J.H.1    Chung, T.D.2    Oldenburg, K.R.3
  • 54
    • 68349112682 scopus 로고    scopus 로고
    • Statistical methods for analysis of high-throughput RNA interference screens
    • doi:nmeth.1351 [pii] 10.1038/nmeth.1351
    • Birmingham, A. et al. Statistical methods for analysis of high-throughput RNA interference screens. Nat Methods 6, 569-575, doi:nmeth.1351 [pii] 10.1038/nmeth.1351 (2009)
    • (2009) Nat Methods , vol.6 , pp. 569-575
    • Birmingham, A.1
  • 55
    • 33749381471 scopus 로고    scopus 로고
    • Analysis of cell-based RNAi screens
    • doi:gb-2006-7-7-R66 [pii] 10.1186/ gb-2006-7-7-R66
    • Boutros, M., Bras, L. P. & Huber, W. Analysis of cell-based RNAi screens. Genome Biol 7, R66, doi:gb-2006-7-7-R66 [pii] 10.1186/ gb-2006-7-7-R66 (2006)
    • (2006) Genome Biol , vol.7
    • Boutros, M.1    Bras, L.P.2    Huber, W.3
  • 56
    • 33749023011 scopus 로고    scopus 로고
    • Minimizing the risk of reporting false positives in large-scale RNAi screens
    • doi:nmeth1006-777 [pii] 10.1038/nmeth 1006-777
    • Echeverri, C. J. et al. Minimizing the risk of reporting false positives in large-scale RNAi screens. Nat Methods 3, 777-779, doi:nmeth1006-777 [pii] 10.1038/nmeth 1006-777 (2006)
    • (2006) Nat Methods , vol.3 , pp. 777-779
    • Echeverri, C.J.1
  • 57
    • 61849127843 scopus 로고    scopus 로고
    • RNA interference screening demystified
    • doi:jcp.2008.058735 [pii] 10.1136/jcp.2008.058735
    • Lord, C. J., Martin, S. A. & Ashworth, A. RNA interference screening demystified. J Clin Pathol 62, 195-200, doi:jcp.2008.058735 [pii] 10.1136/jcp.2008.058735 (2009).
    • (2009) J Clin Pathol , vol.62 , pp. 195-200
    • Lord, C.J.1    Martin, S.A.2    Ashworth, A.3
  • 58
    • 34548740266 scopus 로고    scopus 로고
    • Increasing the robustness and validity of RNAi screens
    • DOI 10.2217/14622416.8.8.1037
    • Haney, S. A. Increasing the robustness and validity of RNAi screens. Pharmacogenomics 8, 1037-1049, doi:10.2217/14622416.8.8.1037 (2007) (Pubitemid 47423691)
    • (2007) Pharmacogenomics , vol.8 , Issue.8 , pp. 1037-1049
    • Haney, S.A.1
  • 59
    • 0033055583 scopus 로고    scopus 로고
    • Miniaturization of a Mammalian Cell- Based Assay: Luciferase Reporter Gene Readout in a 3 Microliter 1536-Well Plate
    • Maffia, A. M., 3rd, Kariv, I. I. & Oldenburg, K. R. Miniaturization of a Mammalian Cell- Based Assay: Luciferase Reporter Gene Readout in a 3 Microliter 1536-Well Plate. J Biomol Screen 4, 137-142 (1999)
    • (1999) J Biomol Screen , vol.4 , pp. 137-142
    • Maffia III, A.M.1    Kariv, I.I.2    Oldenburg, K.R.3
  • 60
    • 67449099369 scopus 로고    scopus 로고
    • Long-term storage of compound solutions for high-throughput screening by using a novel 1536-well microplate
    • doi:1087057109335261 [pii] 10.1177/1087057109335261
    • Pfeifer, M. J. & Scheel, G. Long-term storage of compound solutions for high-throughput screening by using a novel 1536-well microplate. J Biomol Screen 14, 492-498, doi:1087057109335261 [pii] 10.1177/1087057109335261 (2009)
    • (2009) J Biomol Screen , vol.14 , pp. 492-498
    • Pfeifer, M.J.1    Scheel, G.2
  • 61
    • 79952325296 scopus 로고    scopus 로고
    • The evolution of RNAi technologies in the drug discovery business
    • Borawski, J. & Gaither, A. The evolution of RNAi technologies in the drug discovery business. European Pharmaceutical Review 5, 35-39 (2010)
    • (2010) European Pharmaceutical Review , vol.5 , pp. 35-39
    • Borawski, J.1    Gaither, A.2
  • 63
    • 66649137944 scopus 로고    scopus 로고
    • RNAi screen for rapid therapeutic target identification in leukemia patients
    • doi:0903233106 [pii] 10.1073/pnas.0903233106
    • Tyner, J. W. et al. RNAi screen for rapid therapeutic target identification in leukemia patients. Proc Natl Acad Sci U S A 106, 8695-8700, doi:0903233106 [pii] 10.1073/pnas.0903233106 (2009)
    • (2009) Proc Natl Acad Sci U S A , vol.106 , pp. 8695-8700
    • Tyner, J.W.1
  • 64
    • 0016318441 scopus 로고
    • Cellular tumorigenicity in nude mice: Correlation with cell growth in semi-solid medium
    • doi:0092-8674(74)90050-6 [pii]
    • Freedman, V. H. & Shin, S. I. Cellular tumorigenicity in nude mice: correlation with cell growth in semi-solid medium. Cell 3, 355-359, doi:0092-8674(74)90050-6 [pii] (1974)
    • (1974) Cell , vol.3 , pp. 355-359
    • Freedman, V.H.1    Shin, S.I.2
  • 65
    • 35148869637 scopus 로고    scopus 로고
    • A high-throughput soft agar assay for identification of anticancer compound
    • doi:12/7/938 [pii] 10.1177/1087057107306130
    • Anderson, S. N., Towne, D. L., Burns, D. J. & Warrior, U. A high-throughput soft agar assay for identification of anticancer compound. J Biomol Screen 12, 938-945, doi:12/7/938 [pii] 10.1177/1087057107306130 (2007)
    • (2007) J Biomol Screen , vol.12 , pp. 938-945
    • Anderson, S.N.1    Towne, D.L.2    Burns, D.J.3    Warrior, U.4
  • 66
    • 61849180355 scopus 로고    scopus 로고
    • Automated soft agar assay for the high-throughput screening of anticancer compounds
    • doi:S0003-2697(09)00054-2 [pii] 10.1016/j.ab.2009.01.029
    • Thierbach, R. & Steinberg, P. Automated soft agar assay for the high-throughput screening of anticancer compounds. Anal Biochem 387, 318-320, doi:S0003-2697(09)00054-2 [pii] 10.1016/j.ab.2009.01.029 (2009)
    • (2009) Anal Biochem , vol.387 , pp. 318-320
    • Thierbach, R.1    Steinberg, P.2
  • 67
    • 0034676457 scopus 로고    scopus 로고
    • Functional genomic analysis of C. elegans chromosome I by systematic RNA interference
    • doi:10.1038/35042517
    • Fraser, A. G. et al. Functional genomic analysis of C. elegans chromosome I by systematic RNA interference. Nature 408, 325-330, doi:10.1038/35042517 (2000).
    • (2000) Nature , vol.408 , pp. 325-330
    • Fraser, A.G.1
  • 68
    • 0034676448 scopus 로고    scopus 로고
    • Functional genomic analysis of cell division in C. elegans using RNAi of genes on chromosome III
    • doi:10.1038/35042526
    • Gonczy, P. et al. Functional genomic analysis of cell division in C. elegans using RNAi of genes on chromosome III. Nature 408, 331-336, doi:10.1038/35042526 (2000)
    • (2000) Nature , vol.408 , pp. 331-336
    • Gonczy, P.1
  • 69
    • 33646123992 scopus 로고    scopus 로고
    • Secretion of Wnt ligands requires Evi, a conserved transmembrane protein
    • doi:S0092- 8674(06)00451-X [pii] 10.1016/j.cell.2006.04.009
    • Bartscherer, K., Pelte, N., Ingelfinger, D. & Boutros, M. Secretion of Wnt ligands requires Evi, a conserved transmembrane protein. Cell 125, 523-533, doi:S0092- 8674(06)00451-X [pii] 10.1016/j.cell.2006.04.009 (2006)
    • (2006) Cell , vol.125 , pp. 523-533
    • Bartscherer, K.1    Pelte, N.2    Ingelfinger, D.3    Boutros, M.4
  • 71
    • 34447530305 scopus 로고    scopus 로고
    • A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila
    • doi:nature05954 [pii] 10.1038/nature05954
    • Dietzl, G. et al. A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila. Nature 448, 151-156, doi:nature05954 [pii] 10.1038/nature05954 (2007)
    • (2007) Nature , vol.448 , pp. 151-156
    • Dietzl, G.1
  • 72
    • 33646037067 scopus 로고    scopus 로고
    • Drosophila immunity: A large-scale in vivo RNAi screen identifies five serine proteases required for Toll activation
    • doi:S0960-9822(06)01276-0 [pii] 10.1016/j.cub.2006.03.020
    • Kambris, Z. et al. Drosophila immunity: a large-scale in vivo RNAi screen identifies five serine proteases required for Toll activation. Curr Biol 16, 808-813, doi:S0960-9822(06)01276-0 [pii] 10.1016/j.cub.2006.03.020 (2006)
    • (2006) Curr Biol , vol.16 , pp. 808-813
    • Kambris, Z.1
  • 73
    • 34347344980 scopus 로고    scopus 로고
    • Tissue-specific and reversible RNA interference in transgenic mice
    • doi:ng2045 [pii] 10.1038/ng2045
    • Dickins, R. A. et al. Tissue-specific and reversible RNA interference in transgenic mice. Nat Genet 39, 914-921, doi:ng2045 [pii] 10.1038/ng2045 (2007)
    • (2007) Nat Genet , vol.39 , pp. 914-921
    • Dickins, R.A.1
  • 74
    • 56349121125 scopus 로고    scopus 로고
    • An oncogenomics-based in vivo RNAi screen identifies tumor suppressors in liver cancer
    • doi:S0092- 8674(08)01302-0 [pii] 10.1016/j.cell.2008.09.061
    • Zender, L. et al. An oncogenomics-based in vivo RNAi screen identifies tumor suppressors in liver cancer. Cell 135, 852-864, doi:S0092- 8674(08)01302-0 [pii] 10.1016/j.cell.2008.09.061 (2008)
    • (2008) Cell , vol.135 , pp. 852-864
    • Zender, L.1
  • 75
    • 70349454058 scopus 로고    scopus 로고
    • Functional identification of tumor-suppressor genes through an in vivo RNA interference screen in a mouse lymphoma model
    • doi:S1535-6108(09)00262-1 [pii] 10.1016/j.ccr.2009.08.015
    • Bric, A. et al. Functional identification of tumor-suppressor genes through an in vivo RNA interference screen in a mouse lymphoma model. Cancer Cell 16, 324-335, doi:S1535-6108(09)00262-1 [pii] 10.1016/j.ccr.2009.08.015 (2009)
    • (2009) Cancer Cell , vol.16 , pp. 324-335
    • Bric, A.1
  • 76
    • 44849097747 scopus 로고    scopus 로고
    • DLC1 is a chromosome 8p tumor suppressor whose loss promotes hepatocellular carcinoma
    • doi:22/11/1439 [pii] 10.1101/gad.1672608
    • Xue, W. et al. DLC1 is a chromosome 8p tumor suppressor whose loss promotes hepatocellular carcinoma. Genes Dev 22, 1439-1444, doi:22/11/1439 [pii] 10.1101/gad.1672608 (2008)
    • (2008) Genes Dev , vol.22 , pp. 1439-1444
    • Xue, W.1
  • 77
    • 33750824739 scopus 로고    scopus 로고
    • Modeling CTLA4-linked autoimmunity with RNA interference in mice
    • doi:0607854103 [pii] 10.1073/pnas.0607854103
    • Chen, Z., Stockton, J., Mathis, D. & Benoist, C. Modeling CTLA4-linked autoimmunity with RNA interference in mice. Proc Natl Acad Sci U S A 103, 16400-16405, doi:0607854103 [pii] 10.1073/pnas.0607854103 (2006)
    • (2006) Proc Natl Acad Sci U S A , vol.103 , pp. 16400-16405
    • Chen, Z.1    Stockton, J.2    Mathis, D.3    Benoist, C.4


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